Ruminal degradation kinetics, intake, digestibility, and feeding behavior of beef steers offered annual or perennial grass-hay with or without supplementation

2021 ◽  
Author(s):  
J D Sugg ◽  
J O Sarturi ◽  
D D Henry ◽  
F M Ciriaco ◽  
C P West ◽  
...  
Keyword(s):  
Feeding Behavior ◽  
Degradation Kinetics ◽  
Total Concentration ◽  
Perennial Grass ◽  
Latin Square ◽  
Eragrostis Tef ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Grass Hay ◽  
Ruminal Degradation

Abstract Effects of dried distillers grains plus solubles (DDGS) on ruminal fermentation, degradation kinetics, and feeding behavior of steers offered annual (Eragrostis tef; TEFF) or perennial (Bothriochloa bladhii; OWB) grass-hay were evaluated. Ruminally-cannulated Angus crossbred steers [n = 6; body weight (BW) = 304 ± 11 kg] were assigned to a 4 × 6 unbalanced Latin square design with 4 treatments arranged as a 2 × 2 factorial: hay type (OWB or TEFF) and DDGS supplementation {0 or 0.5% BW [dry matter (DM) basis]}. Steers had ad libitum access to hay. Periods consisted of a 14-d adaptation followed by 7 d of collection. Residues from the in situ incubations (0, 3, 6, 12, 24, 36, 48, 72, and 96 h post-feeding) were fitted to a first-order kinetics model using the NLIN procedure of SAS. The DDGS decreased (P < 0.01) TEFF DM intake (DMI) by 11.3%, while not affecting DMI of OWB. The greatest DMI was observed for steers supplemented with DDGS, regardless of forage, and least in steers consuming OWB without DDGS (hay type × DDGS; P = 0.03). Non-supplemented steers spent more (P < 0.01) time eating hay. Digestibility of DM tended (P = 0.06) to increase with DDGS supplementation. A hay type × DDGS interaction was observed (P ≤ 0.05) on ruminal effective degradable fractions. The rate of degradation, soluble fraction, and the potentially degradable fraction of OM, NDF, and ADF increased (P ≤ 0.05), while the undegradable fraction of all components decreased (P ≤ 0.01) when steers were offered TEFF compared to OWB. Ruminal DM, OM, and ADF degradation lag-time increased (P ≤ 0.02) in steers offered OWB. Ruminal degradation kinetics were not (P ≥ 0.17) independently affected by DDGS supplementation. Average ruminal pH of steers offered TEFF (P < 0.01) and those offered DDGS (P < 0.01) were lower than OWB and non-supplemented steers. Total concentration of VFA tended (P = 0.09) to increase when DDGS was provided with OWB, while decreasing when TEFF was offered. The acetate:propionate increased (P < 0.01) with DDGS supplementation due to a decrease (P = 0.03) in propionate. Ruminal NH3-N was greater (P = 0.03) in steers offered TEFF compared to OWB, and those supplemented with DDGS (P = 0.03). An annual, in place of a conventional, perennial hay improved intake and digestion of nutrients, without affecting feeding behavior. The supplementation with DDGS appears to affect forage intake, ruminal degradation, and feeding behavior, although not independent of forage quality.

Ruminal Degradation Kinetics of Beef Steers Fed Annual or Perennial Grass-hay with or Without Supplementation

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 29-30
Author(s):  
Kaue T Nardi ◽  
Jhones O Sarturi ◽  
Joel D Sugg ◽  
Darren D Henry ◽  
Francine M Ciriaco ◽  
...  
Keyword(s):  
Degradation Kinetics ◽  
Perennial Grass ◽  
Latin Square ◽  
Beef Steers ◽  
Dried Distillers Grains ◽  
First Order Kinetics ◽  
Grass Hay ◽  
Ruminal Degradation ◽  
Mean Differences ◽  
Kinetics Of

Abstract The effects of dried distillers grains plus solubles (DDGS) supplementation on ruminal degradation kinetics of steers fed annual [‘Tiffany’Eragrostis tef (Zucc.) Trotter; TEFF] or perennial [‘WW-B Dahl’ Old World bluestem, Bothriochloa bladhii (Retz).T. Blake; OWB] grass hay were evaluated. Ruminally cannulated crossbred-Angus steers (n = 6; BW = 304 ± 11 kg) were assigned to a 4 × 6 unbalanced Latin square design with 4 treatments. Treatments were arranged as a 2 × 2 factorial, with factors being hay type (OWB or TEFF) and DDGS supplementation [0 or 0.5% BW (DM basis)]. Steers had ad libitum access to hay and received 110 g of a mineral/vitamin packet daily. Periods consisted of a 14-d adaptation followed by 7 d of collection. Hay types were reverse-incubated at 0, 3, 6, 12, 24, 36, 48, 72, and 96 h post-feeding. Residues from the in situ incubations were fitted to a first-order kinetics model using the non-linear procedure of SAS. The GLIMMIX procedures of SAS were used for the statistical analysis. A hay type × DDGS interaction was observed (P ≤ 0.05) for the estimation of substrate effective degradable fraction of OM, NDF, ADF, and hemicellulose; however, after adjusting with Tukey’s, there were no treatment mean differences (P ≥ 0.08). The rate of digestion, soluble fraction, and the potentially degradable fraction of OM, NDF, ADF, and hemicellulose increased (P ≤ 0.05), while the undegradable fraction decreased (P ≤ 0.01) when steers were fed TEFF, regardless of DDGS supplementation. Ruminal degradation lag-time increased (P ≤ 0.02) by 1.5, 1.45, and 2.6 h for DM, OM, and ADF, respectively in steers fed OWB, regardless of DDGS supplementation. Ruminal degradation kinetics were not (P ≥ 0.17) independently affected by DDGS supplementation. Future investigations should elucidate the interaction between supplementation and forage quality.

scholarly journals PSXIV-41 Warm-season annual or perennial hay fed to growing beef steers with or without dried distillers grains supplementation: ruminal fermentation characteristics, digestion, and feeding behavior

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 438-439
Author(s):  
Joel D Sugg ◽  
Carly A Hoffmann ◽  
Lucas B Kondratovich
Keyword(s):  
Feeding Behavior ◽  
Rumen Fluid ◽  
Latin Square ◽  
Protein Supplementation ◽  
Eragrostis Tef ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Ruminal Ph ◽  
Ruminal Digestion

Abstract The effects of hay type and protein supplementation on intake, feeding behavior, nutrient digestion, and ruminal digestion characteristics were evaluated. Ruminally cannulated Angus beef steers (n = 6; BW = 304 kg ± 11 kg) were randomly assigned within a sequence of treatments using a 4 × 6 unbalanced Latin square design (6 steers; and 4 diets; fed once-daily). A 2 x 2 factorial treatment arrangement was used as follows: 1) ‘WW-B. Dahl’ Old World bluestem [Bothriochloa bladhii (Retz) S.T. Blake; WWBD or Eragrostis tef (Zucc.) Trotter; TEFF); and 2) dried distillers grain (DDGS) at 0 or 0.5% BW. Each period consisted of a 14-d of adaptation and 7-d collection. Steers were observed (5-min intervals, 24 h) for behavioral assessment; while ruminal pH was continuously measured (wireless pH probe), and ruminal fluid collected at 0, 2, 4, 8, and 16 h after-feeding. Steers fed TEFF hay and those fed DDGS (both, P = 0.04) had greater DMI compared to WWBD and not supplemented. Chewing activity did not differ (P ≥ 0.54). Non-supplemented steers spent more time eating hay (P < 0.01) than supplemented steers. Average ruminal pH of TEFF (6.32) was lower (P > 0.01) than WWBD (6.56). Non-supplemented steers produced less in vitro total gas and methane (both, P = 0.02) per g rumen fluid DM. The VFA profile was not affected (P ≥ 0.45) by treatments. Apparent total-tract digestibility and ruminal degradation (P ≤ 0.01) were greater with TEFF fed steers than WWBD. Hemicellulose digestion was reduced by 6.95% (P = 0.03) with DDGS supplementation. An annual hay in place of a conventional perennial hay improved intake, ruminal digestion of nutrients, without affecting feeding behavior, while supplementation with DDGS reduced forage intake time and quantity, without negatively affecting ruminal fiber digestion.

Effects of roughage type on particle separation, rumination, fiber mat characteristics, in situ degradation, and ruminal fermentation parameters in beef steers

2021 ◽  
Author(s):  
Catherine L Lockard ◽  
Caleb G Lockard ◽  
Wyatt N Smith ◽  
Kendall J Karr ◽  
Ben P Holland ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Fluid Collection ◽  
Latin Square ◽  
Experimental Unit ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
In Situ Degradation ◽  
Fermentation Parameters

Abstract Six ruminally cannulated steers (average BW = 791 + 71 kg) were used in a replicated 3 × 3 Latin square experiment to determine the effects of roughage type on rumination, fiber mat characteristics, and rumen fermentation variables. Three roughages were included at 7% (DM basis) in a steam flaked corn-based diet; cotton burrs (CB), wheat silage (WS), or corn stalks (CS). Steers were fitted with a sensory collar to record rumination behaviors in 2-h intervals at the beginning of the experiment. Each 30-d period consisted of a 7-d of recovery, 14-d of diet adaptation, 7-d of rumination data collection (daily and bi-hourly average rumination), 1-d of rumen fluid collection, and 1-d of rumen evacuations. In situ degradation of individual roughages was determined for 4-d after period 3 evacuations. During rumen evacuations, ruminal contents were removed; the rumen fiber mat (RF) was separated from the liquid portion with a 2 mm sieve, weighed, and a subsample was dried. Data were analyzed using the MIXED procedure of SAS with steer as the experimental unit and roughage (CB, WS, and CS) as the main effect. Dry matter intake (DMI) was not different for CB and WS (P = 0.25) and greatest for steers consuming CS diet (P  < 0.01). Roughage type did not influence the weight of the RF dry matter (%; DM; P = 0.92), RF weight (P = 0.69), or RF:DMI ratio (P = 0.29). Daily rumination (min/d) did not differ among roughages (P = 0.40), but min of rumination/kg of DMI was greatest for CS (18.0 min), min/kg of NDF was greatest for WS (89.8 min; P = 0.02), and min/kg of peNDF was greatest for CS (132.4 min; P  < 0.01). Wheat silage had the greatest percentage of soluble DM and CB-R and CS-R (P  < 0.01) had the greatest ruminal degraded DM fraction. Rumen fiber mat did not differ for roughages, although rumination min/kg of DMI and peNDF was greatest for steers consuming CS and WS. In situ degradation determined that CB-R and CS-R had the greatest percentage of ruminal degraded DM. Based on the objective of the experiment, roughage type did not influence daily rumination or fiber mat characteristics.

Effect of leucaena supplementation level, and provision of urea, on utilisation by sheep of Rhodes grass hay

2001 ◽  
Vol 41 (2) ◽  
pp. 155 ◽  
Author(s):  
I. W. Karda ◽  
G. McL. Dryden
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rhodes Grass ◽  
Microbial Nitrogen ◽  
Grass Hay ◽  
Digestible Organic Matter ◽  
Ruminal Degradation ◽  
Chloris Gayana ◽  
Dietary Treatments

Tarramba leucaena (Leucaena leucocephala cv. Tarramba) foliage had per kilogram dry matter, 169 g protein and 29.8 g condensed tannins. Its value as a supplement, given either with or without urea, to sheep given a low-quality Callide Rhodes grass (Chloris gayana cv. Callide) hay was studied. Six rumen fistulated sheep (mean s.d. liveweight, 34 1.4 kg) were used to compare 6 dietary treatments in an incomplete latin square design. Rhodes grass hay was given ad libitum either alone, or with urea 7 g/day (U), or with leucaena 150 g/day (L150), or leucaena with urea (L150U), or leucaena 300 g/day (L300), or leucaena with urea (L300U). Digestible organic matter intake was increased significantly by leucaena supplementation although digestibility of the whole diet did not alter. Rumen fluid ammonia-N was not altered by leucaena supplementation, but was increased by urea. This suggests that Tarramba foliage protein has some resistance to ruminal degradation. Liquid and solids passage rates were not affected by the treatments. Microbial nitrogen supply to the intestine (g/day), and the efficiency of microbial nitrogen synthesis (g/kg organic matter apparently digested in the rumen), were increased by leucaena supplementation (P<0.01). Microbial protein synthesis was the only response in which 300 g/day air-dry Tarramba foliage gave improved results over 150 g/day.

scholarly journals Methane Emission of Cattle Fed Urochloa brizantha Hay Harvested at Different Stages

2015 ◽  
Vol 8 (1) ◽  
pp. 163
Author(s):  
C. F. M. Nascimento ◽  
A. Berndt ◽  
L. A. Romero Solorzano ◽  
P. M. Meyer ◽  
R. T. S. Frighetto ◽  
...  
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Sulfur Hexafluoride ◽  
Total Concentration ◽  
Latin Square ◽  
Short Chain Fatty Acids ◽  
Growth Stages ◽  
Ruminal Fermentation ◽  
Linear Effect ◽  
Urochloa Brizantha

<p>The objective of this trial was to assess the methane emission rate, as well as the digestibility and ruminal fermentation parameters in animals fed <em>Urochloa brizantha</em> hay harvested at different stages of growth. Six Nellore rumen-cannulated steers (402.0 ± 51.62 kg) were assigned to a replicated 3×3 Latin square (18 experimental units). Treatments differed according to three growth stages of the forage: 15 - hay with 15 days of growth, 45 - hay with 45 days of growth and 90 - hay with 90 days of growth. Each experimental period lasted 21 days. From the 8<sup>th</sup> day, feces were sampled during 5 days to determine apparent total digestibility of dry matter and its fractions. Beginning on the 13<sup>th</sup> day and running for 7 days, methane production was evaluated using the sulfur hexafluoride (SF<sub>6</sub>) tracer technique. On the 20<sup>th</sup> day, ruminal fluid was sampled prior to and after 2, 4, 6, 8 and 10 hours post-morning feeding for evaluating pH, ammonia-N and short-chain fatty acids (SCFA). As the stage of forage growth for harvesting advanced in days, the digestibility coefficient of crude protein decreased and non-fibrous carbohydrate values increased. Methane production was affected not only by the stage of forage growth, but also by quality of its conservation after harvesting. There were significant differences among treatments when methane was expressed in g kg<sup>-1</sup> DMI, g kg<sup>-1</sup> OMI, % GE and % DE, with non-linear effect. The stage of forage growth did not influence ruminal pH, total concentration or molar proportion of SCFA, but decreased NH<sub>3</sub>-N concentration.</p>

scholarly journals Effects of Hybrid and Grain Maturity Stage on the Ruminal Degradation and the Nutritive Value of Maize Forage for Silage

Agriculture ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 251
Author(s):  
Egon Henrique Horst ◽  
Secundino López ◽  
Mikael Neumann ◽  
Francisco Javier Giráldez ◽  
Valter Harry Bumbieris Junior
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Degradation Kinetics ◽  
Gas Production ◽  
Maturity Stage ◽  
Ruminal Fermentation ◽  
Maturity Stages ◽  
Whole Plant ◽  
Ruminal Degradation

The study aimed to examine the effects of harvesting three maize hybrids at different maturity stages on the ruminal fermentation kinetics, fermentation end-products (volatile fatty acid, ammonia and methane) output, and digestibility of maize plant fractions, as well as the degradability of the resulting silage. Three hybrids were compared (Maximus VIP3, Defender VIP and Feroz VIP) harvested at three grain maturity stages (milk (R3), dough (R4) and dent (R5) grain), while silage samples were collected only at the dent grain stage (R5). Grain digestibility tended to decrease as the maturity stage progressed (p < 0.05), while the whole-plant digestibility increased with increasing maturity for the three evaluated hybrids (p < 0.05). The gas production of whole-plant at 24 h of incubation was higher for the Maximus hybrid than for the others (p < 0.05), with average values of 188, 196 and 207 mL g−1 dry matter at stages R3, R4 and R5, respectively. For the in situ ruminal degradation kinetics of silage, instantly degradable dry matter and fiber potential degradability were greater with Maximus maize forage than with the other two hybrids. From the perspective of in vitro results, the Maximus VIP3 hybrid seems to be the most suitable for silage production when harvested between the dough and the dent grain stage of maturity.

Canola screenings as a fiber source in barley-based feedlot diets: effects on rumen fermentation and performance of steers

2000 ◽  
Vol 80 (1) ◽  
pp. 161-168 ◽  
Author(s):  
S. J. Pylot ◽  
J. J. McKinnon ◽  
T. A. McAllister ◽  
A. F. Mustafa ◽  
J. Popp ◽  
...  
Keyword(s):  
Control Diet ◽  
Latin Square ◽  
Dietary Treatment ◽  
Barley Grain ◽  
Feedlot Cattle ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Finishing Diets ◽  
And Performance ◽  
Barley Silage

Two experiments were conducted to determine the feeding value of canola screenings in combination with barley grain for beef steers. Four dietary treatments were used. These included canola screenings:barley grain ratios of 100:0; 75:25, 50:50, and 25:75 (as-fed basis). In a metabolic trial, the effects of dietary treatment on ruminal fermentation parameters were determined in a 4 × 4 Latin square design experiment using four ruminally fistulated steers. In a production trial, 66 individually fed steers were used in an 83-d finishing trial to determine the performance and carcass characteristics of feedlot cattle fed different levels of canola screenings. A barley grain/barley silage-based control diet was also fed for comparison purposes. Ruminal pH decreased (P < 0.05) while total volatile fatty acid concentrations increased (P < 0.05) as the level of barley grain in the diet increased. Ruminal NH3–N followed a pattern (P < 0.05) similar to that of pH. Inclusion level of canola screenings had no effect on DM intake. However, ADG and feed efficiency increased (P < 0.05) as the level of barley grain in the diet increased. Increasing the level of barley grain in the finishing diet decreased (P < 0.05) lean meat yield and increased (P < 0.05) carcass fat. It was concluded that canola screenings can be included as a source of fiber in barley-based diets. However, levels in excess of 500 g kg−1 reduced the performance of feedlot steers. Performance and cost of gain with 250 and 500 g kg−1 canola screenings in barley-based finishing diets were comparable with those fed an 800 g kg−1 barley grain and 200 g kg−1 barley silage diet. Key words: Canola screenings, ruminal fermentation, feedlot steer performance

Ruminal fermentation, methanogenesis and nitrogen utilization of sheep receiving tropical grass hay-concentrate diets offered with Sapindus saponaria fruits and Cratylia argentea foliage

2004 ◽  
Vol 79 (1) ◽  
pp. 177-189 ◽  
Author(s):  
H. D. Hess ◽  
R. A. Beuret ◽  
M. Lötscher ◽  
I. K. Hindrichsen ◽  
A. Machmüller ◽  
...  
Keyword(s):  
Latin Square ◽  
Basal Diet ◽  
Open Circuit ◽  
Ruminal Fermentation ◽  
Body Protein ◽  
Protein Retention ◽  
Methane Release ◽  
Grass Hay ◽  
Sapindus Saponaria ◽  
Tropical Grass

AbstractThe effects of supplementing a tropical, low-quality grass hay (Brachiaria dictyoneura) with legume foliage (Cratylia argentea) or fruits of the multipurpose tree Sapindus saponaria on ruminal fermentation, methane release and nitrogen (N) utilization were evaluated. Six Swiss White Hill lambs were used in a 6 ✕ 6 Latin-square design with a 3 ✕ 2 factorial arrangement of treatments with measurements of energy metabolism being conducted using open-circuit respiratory chambers. Treatments consisted of three basal diets, either grass alone or legume: grass ratios of 1: 2 or 2: 1. These basal diets were supplemented (1: 3) with a control concentrate or with a concentrate containing 250 g/kg dry matter of S. saponaria fruits. The apparent total tract digestibilities of organic matter (OM) and neutral-detergent fibre (NDF) were reduced and the proportionate crude protein (CP) losses through faeces were increased (P 0·01) by supplementation with S. saponaria, and digestibilities of OM and NDF were linearly reduced (P 0·001) with increasing legume proportion. Body energy retention, however, was similar in all diets. Along with CP intake, the proportionate CP losses through faeces decreased (P 0·001) with increasing legume proportion which was associated with improved (P 0·001) body protein retention and reduced (P 0·1) fat retention. Ruminal fluid ammonia concentration was not significantly affected (P > 0·1) by the inclusion of S. saponaria in the concentrate, but increased linearly (P 0·001) as dietary legume proportion was elevated. Supplementation with fruits of S. saponaria increased (P 0·01) total bacteria count, and decreased (P 0·001) total ciliate protozoa count by more than proportionately 0·50. Daily methane release was reduced (P 0·01) by S. saponaria supplementation in all basal diet types. Although being not clearly affected on a daily basis, methane release relative to body protein retention decreased linearly (P 0·05) with increasing legume proportion. The fact that interactions were mostly non-significant (P > 0·05) indicates that supplementation with S. saponaria fruits is a useful means to reduce methane emission from sheep given both tropical grass-based and grass-legume-based diets. Likewise, including legumes in N-limited tropical diets seems to represent an environmentally friendly way to improve animal productivity.

EFFECT OF CHOLINE IN ALL-CONCENTRATE DIETS OF FEEDLOT STEERS AND ON RUMINAL ACIDOSIS

1985 ◽  
Vol 65 (1) ◽  
pp. 135-146 ◽  
Author(s):  
T. S. RUMSEY
Keyword(s):  
Lactic Acid ◽  
Lactic Acidosis ◽  
Latin Square ◽  
Serum Glucose ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Plasma Urea ◽  
Ruminal Acidosis ◽  
Feedlot Steers ◽  
Made In

Four trials with beef steers were conducted to determine if choline supplementation of an all-concentrate diet improved performance and affected ruminal lactic acidosis. Trial 1 was a preliminary feeding trial with 12 individually fed steers averaging 356 kg initial body weight. Trials 2 and 3 were feedlot trials with 64 and 80 steers averaging 359 and 320 kg initial body weights, respectively. Trial 4 was an intensive trial with three ruminal-fistulated steers in a 3 × 3 Latin square design in which subacute ruminal acidosis was produced. Choline supplementation neither consistently affected feedlot performance and carcass merit measurements in the feeding trials, nor consistently affected end products of ruminal fermentation. Choline appeared to reduce total plasma amino acids and increase plasma urea (measurements made in Trials 2 and 3) and reduce serum glucose, triglycerides and glutamic pyruvic transaminase while increasing serum total lipids and cholesterol (measurements made in Trial 3). Choline did not affect ruminal volatile fatty acid, pH, ammonia and lactic acid isomer changes associated with subacute lactic acidosis. The results indicate that the levels of choline supplementation of an all-concentrate diet for beef steers used in this study did not affect ruminal acidosis and did not improve performance of feedlot steers. Key words: Choline, acidosis, lactic acid, rumen, steer, beef cattle

scholarly journals PSII-6 Comparative effects of two multi-species direct-fed microbial products on rumen fermentation, bacterial community and metabolome of beef steers

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 398-398
Author(s):  
Ibukun M Ogunade ◽  
Devan M Compart ◽  
Andres A Pech-Cervantes
Keyword(s):  
Bacillus Subtilis ◽  
Bacterial Community ◽  
Relative Abundance ◽  
Isotope Labeling ◽  
Latin Square ◽  
Basal Diet ◽  
Beef Steers ◽  
Ruminal Fermentation ◽  
Metabolome Analysis ◽  
Fermentation Products

Abstract We examined the effects of two direct-fed microbials (DFM) containing multiple microbial species and their fermentation products on ruminal fermentation, bacterial community and metabolome of beef steers. Nine rumen-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) CON (basal diet without additive), (2) PROB (basal diet plus 19 g/d of Commence), and (3) SYNB (basal diet plus 28 g/d of RX3). Commence is a blend of active S. cerevisiae, Enterococcus lactis, Bacillus subtilis, Enterococcus faecium, and L. casei, and their fermentation products. RX3 is a blend of active S. cerevisiae and the fermentation products of S. cerevisiae, Enterococcus lactis, Bacillus licheniformis, and Bacillus subtilis. Daily dry matter intake was calculated. On d 21 of each period, ruminal contents were sampled at 3, 6, 12, and 18 h after feeding for analysis of VFA, bacterial community via 16S rRNA sequencing, and metabolome via chemical isotope labeling liquid chromatography mass spectrometry. There was no effect on DMI. Compared to CON, supplementation of either PROB or SYNB increased or tended to increase (P ≤ 0.10) total VFA, propionate, and valerate concentrations. Compared to CON, PROB reduced (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, but increased those of Rikenellaceae RC9, Succinivibrionaceae UCG-001, Succiniclasticum, Ruminococcaceae UCG-014, and Ruminococcaceae UCG-002, whereas SYNB decreased (P ≤ 0.05) the relative abundance of Prevotella 1 and Prevotellaceae UCG-001, and increased those of Prevotella 7, Succinivibrio, Succiniclasticum, and Ruminococcaceae UCG-014. Metabolome analysis revealed that 8 ruminal metabolites, including some amino acids, were increased (P ≤ 0.05) by PROB, whereas no differences were found for SYNB. This study demonstrated that supplemental PROB or SYNB altered the ruminal bacterial community and metabolome differently to achieve a similar ruminal fermentation pattern.

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