PSXI-22 Study of biosorption mechanisms of movable forms of lead by soil isolates of the genus Bacillus in experiments in vitro

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 348-349
Author(s):  
Nadezhda Terekhova ◽  
Lyudmila Galaktionova ◽  
Alexey Sizentsov ◽  
Olga Davydova ◽  
Hike Nikiyan
Keyword(s):  
Stationary Growth Phase ◽  
Genus Bacillus ◽  
Scientific Center ◽  
Agricultural Technologies ◽  
Scientific Equipment ◽  
Significant Area ◽  
Lead Levels ◽  
Scientific Results ◽  
Grazing Lands

Abstract The research was supported by the Ministry of Science and Higher Education in accordance with the state assignment for Ural State Mining University No. 0833-2020-0008 ‘Development and environmental and economic substantiation of the technology for reclamation of land disturbed by the mining and metallurgical complex based on reclamation materials and fertilizers of a new type’. We obtain the scientific results by using funds of the Center for the collective use of scientific equipment of the Federal Scientific Center of biological systems and agricultural technologies of RAS as well (No Ross RU.0001.21 PF59, the Unified Russian Register of Centers for Collective Use - http://www.ckp-rf.ru/ckp/77384).The search for natural bioremediators to restore crop and grazing lands in conditions of the increased anthropogenic load is a relevant and promisingly significant area of research. Thus, we were tasked to assess the prospect of using the physiological and adaptive characteristics of microorganisms of the genus Bacillus as an active biosorbent of movable forms of lead from the substrate on the in vitro model.Soil isolates of microorganisms of the genus Bacillus extracted from samples from areas with increased lead levels were used to achieve our aim. Pb(NO3)2 solutions in the concentration range from 1 to 0.063M were used as the factor regulating the growth. Methods used in the work were: the method of agar basins, the nephelometric method, atomic-absorption spectroscopy, and atomic force microscopy. B. subtilis and B. cereus were isolated from soil samples. The maximum resistance to different concentrations of Pb(NO3)2 was revealed for B. subtilis. The presence of lead cations in the nutrient substrate significantly increased the population density to 26.0%. At the same time, the percentage of lead sorption in the initial stage of the stationary growth phase was 65.3% with the maximum localization on the surface structures of the cell membrane.

scholarly journals Estimation of the biosorption potential of certain representatives of the genus Bacillus in interaction with lead cation in vitro

2021 ◽  
Author(s):  
Lyudmila Galaktionova ◽  
Alexey Nikolaevich Sizentsov ◽  
Olga Konstantinovna Davydova ◽  
Hike Nikolaevich Nikiyan ◽  
Yaroslav Alekseevich Sizentsov
Keyword(s):  
Optical Density ◽  
Anthropogenic Pollution ◽  
Atomic Absorption Spectrophotometry ◽  
Stationary Growth Phase ◽  
Inhibitory Effect ◽  
The Body ◽  
Genus Bacillus ◽  
Test Organisms ◽  
High Sorption

The article presents data on the physicochemical and metabolically dependent mechanisms of detoxification by microorganisms of heavy metals that enter the environment during anthropogenic pollution. The taxonomic and physiological-biochemical diversity of microorganisms capable of neutralizing toxicants has been demonstrated. In the experimental part of the study, the combination of the methods used made it possible to fully assess the degree of toxicity and the effect of lead cations on the growth of bacteria of the genus Bacillus in a model experiment. Thus, the use of atomic absorption spectrophotometry and atomic force microscopy gave an idea of the level of biosorption of a given xenobiotic element from a substrate with localization of inactive forms of lead. The data obtained indicate the presence of an inhibitory effect of Pb (NO3) 2 and Pb (CH3COO) 2 in concentrations from 1 mM to 0.063 mM about the test organisms under study. The presence of cations with a nutrient substrate gives a false-positive idea of the degree of influence of lead on the growth of the studied microorganisms, since an increase in optical density in test samples is due to high sorption characteristics and, as a consequence, is characterized by an increase in relative optical density. An assessment of the detoxification mechanisms, which is expressed by active bioaccumulation of lead on the surface elements of the microbial cell up to 65% at the point of entering the stationary growth phase, indicates the promising use of representatives of this group of microorganisms as microbial bioremediation and correctors of excess content of this element in the body or ecosystem as a whole.

scholarly journals Люминесценция кристаллов BaBrI, активированных ионами Ce-=SUP=-3+-=/SUP=-

2019 ◽  
Vol 61 (5) ◽  
pp. 930
Author(s):  
Р.Ю. Шендрик ◽  
И.И. Ковалев ◽  
А.И. Русаков ◽  
Ю.В. Сокольникова ◽  
А.А. Шалаев
Keyword(s):  
Luminescence Spectra ◽  
Russian Science ◽  
Scientific Center ◽  
X Ray ◽  
Trivalent Lanthanides ◽  
Scientific Equipment ◽  
The Government ◽  
Geochemical Studies ◽  
Transfer Mechanisms

The optical properties of BaBrI crystals doped with cerium ions were studied. The possible types of cerium-containing luminescence centers are established. Based on the experimental data obtained for trivalent lanthanides, a Dorenbos diagram was constructed. According to the results of the study of the luminescence spectra measured under X-ray excitation, possible energy transfer mechanisms are assumed. This work was supported by the Russian Science Foundation, a grant of the Russian Science Foundation 17-72-10084: spectroscopy of cerium and cerium-oxygen centers, determination of cerium concentration in crystals, analysis of the results obtained. Some of the crystals investigated in the article were grown as part of the government assignment 0350-2016-0024. The data in the work were obtained using the scientific equipment of the Collective Scientific Center "Isotope-geochemical studies" of the IHP SB RAS.

scholarly journals A Review on in vitro and in vivo Bioremediation Potential of Environmental and Probiotic Species of Bacillus and other Probiotic Microorganisms for Two Heavy Metals, Cadmium and Nickel

2019 ◽  
Vol 16 (1) ◽  
pp. 01-13 ◽  
Author(s):  
Pragya Goyal ◽  
Pranoti Belapurkar ◽  
Anand Kar
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Industrial Effluents ◽  
Eukaryotic Cell ◽  
Genus Bacillus ◽  
Form Of Life ◽  
Heavy Metal Remediation ◽  
Remediation Strategies

Microbial assisted remediation is the ray of hope in the current scenario of tremendous heavy metal pollution. The indiscriminate release of heavy metal laden industrial effluents in the water bodies and soil is now manifesting itself in the form of life threatening health hazards to humans. The conventional heavy metal remediation strategies are not only expensive but are ineffective in low metal concentrations. Microbial assisted remediation of heavy metals has come forward as the cheap and easy alternative. Amongst the various bacterial genera actively involved in bioremediation of cadmium and nickel in the environment, genus Bacillus has shown remarkable ability in this respect owing to its various biochemical and genetic pathways. It can perform bioremediation using multiple mechanisms including biosorption and bioaccumulation. This genus has also been able to reduce toxicity caused by cadmium and nickel in eukaryotic cell lines and in mice, a property also found in probiotic genera like Lactobacillus and Bifidobacterium. This paper reviews the role of environmentally present and known probiotic species of genus Bacillus along with different probiotic genera for their various mechanisms involved for remediation of cadmium and nickel.

Activity of Bacillus amyloliquefaciens MBI600 against soybean bacterioses

2021 ◽  
Author(s):  
R.I. Tarakanov ◽  
◽  
P.A. Vasilyev ◽  
K.S. Troshin ◽  
F.-S. U. Dzhalilov
Keyword(s):  
High Activity ◽  
Bacillus Amyloliquefaciens ◽  
Bacterial Blight ◽  
Genus Bacillus

the article presents data on the effectiveness of biological preparations based on bacteria of the genus Bacillus against pathogens of soybean bacteriosis. The results show high activity of the biofungicide based on Bacillus amyloliquefaciens MBI600 both in vitro and in the vegetative experiment against bacterial blight.

scholarly journals A Novel Point Mutation Promotes Growth Phase-Dependent Daptomycin Tolerance in Staphylococcus aureus

2015 ◽  
Vol 59 (9) ◽  
pp. 5366-5376 ◽  
Author(s):  
Lukas Mechler ◽  
Alexander Herbig ◽  
Kerstin Paprotka ◽  
Martin Fraunholz ◽  
Kay Nieselt ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Point Mutation ◽  
Growth Phase ◽  
Drug Tolerance ◽  
Stationary Growth Phase ◽  
Cellular Functions ◽  
Upstream Gene ◽  
Content Type ◽  
High Degree

ABSTRACTRecalcitrance of genetically susceptible bacteria to antibiotic killing is a hallmark of bacterial drug tolerance. This phenomenon is prevalent in biofilms, persisters, and also planktonic cells and is associated with chronic or relapsing infections with pathogens such asStaphylococcus aureus. Here we report thein vitroevolution of anS. aureusstrain that exhibits a high degree of nonsusceptibility to daptomycin as a result of cyclic challenges with bactericidal concentrations of the drug. This phenotype was attributed to stationary growth phase-dependent drug tolerance and was clearly distinguished from resistance. The underlying genetic basis was revealed to be an adaptive point mutation in the putative inorganic phosphate (Pi) transporter genepitA. Drug tolerance caused by this allele, termedpitA6, was abrogated when the upstream genepitRwas inactivated. Enhanced tolerance toward daptomycin, as well as the acyldepsipeptide antibiotic ADEP4 and various combinations of other drugs, was accompanied by elevated intracellular concentrations of Piand polyphosphate, which may reversibly interfere with critical cellular functions. The evolved strain displayed increased rates of survival within human endothelial cells, demonstrating the correlation of intracellular persistence and drug tolerance. These findings will be useful for further investigations ofS. aureusdrug tolerance, toward the development of additional antipersister compounds and strategies.

Integrative Approach to the Evaluation of Implementation Research Projects: Siberian Branch of the Russian Academy of Sciences

2019 ◽  
Vol 6 (6) ◽  
pp. 28-41
Author(s):  
Галина Унтура ◽  
Galina Untura ◽  
Татьяна Новикова ◽  
Tatyana Novikova ◽  
Наталья Горбачева ◽  
...  
Keyword(s):  
New Technologies ◽  
Basic Research ◽  
Integrative Approach ◽  
Research Projects ◽  
Scientific Center ◽  
Education Production ◽  
Project Analysis ◽  
Integrative Assessment ◽  
Scientific Results ◽  
Interdisciplinary Projects

The article is devoted to the methodological foundations of an integrative approach to managing and evaluating the effectiveness of interdisciplinary projects with many participants. The aim of the study is to assess the effectiveness of interdisciplinary projects of the Novosibirsk Scientific Center in accordance with the methodology of the integrative approach, which takes into account the multi-purpose use of the results of research and development of a specific scientific direction in different fields (science, education, production). The essence of the integrative assessment of projects consists in the integrated application of the methods of the typology of scientific results and methods of project analysis, which allows to take into account the totality of the obtained results of an interdisciplinary project, starting from the stage of basic research and ending with the development stages of product prototypes or new technologies of high readiness. The analysis of the characteristics and effects of the projects that we use in the assessment methodology is carried out from the standpoint of different criteria: the attainability of world-class scientific results; increase the level of technology readiness, the use of scientific results in education and production. Innovative opportunities of projects can be additionally economically evaluated by methods of project analysis. The examples of the success of projects in terms of their high applicability for the development of priority areas of science and technology, improving the quality of education are shown. The findings can be used to develop a strategy for the development of regional research centers where research institutes of different profiles are located and in the management of research projects with many participants.

Neutrophil Functions in Lead-Exposed Workers

1990 ◽  
Vol 9 (4) ◽  
pp. 231-233 ◽  
Author(s):  
A. Bergeret ◽  
E. pouget ◽  
R. Tedone ◽  
T. Meygret ◽  
R. Cadot ◽  
...  
Keyword(s):  
Blood Lead ◽  
Ethnic Origin ◽  
Drug Intake ◽  
Smoking Habits ◽  
Blood Lead Levels ◽  
Exposed Workers ◽  
Clinical Consequences ◽  
Lead Levels

Neutrophil functions were studied in 38 lead-exposed workers compared to 34 controls. Both groups were matched according to age, sex, drinking and smoking habits, ethnic origin and drug intake. Blood lead levels were found to be seven times higher in exposed workers than in controls. Phagocytosis assayed by chemiluminescence was found to be slightly but not significantly altered in exposed workers. In contrast, chemotaxis using the agarose technique was significantly depressed. These results are in agreement with previous in-vitro findings. A further assessment of clinical consequences is warranted.

scholarly journals Evaluación in vitro de la formación de biopelículas de bacterias marinas del Caribe colombiano

2019 ◽  
Vol 48 (2) ◽  
Author(s):  
Sofia López Pérez ◽  
Sven Zea ◽  
Javier Gómez
Keyword(s):  
Bacterial Communities ◽  
Biofilm Formation ◽  
Important Process ◽  
Genus Bacillus ◽  
Violet Crystal ◽  
16S Rna ◽  
Different Temperatures ◽  
Environmental Variations ◽  
Biofilm Quantification

Biofilm formation is an important process for marine bacterial communities because this mechanism favors adaptation to variations in environmental conditions. The objective of this study was to evaluate the biofilm formation of bacteria isolated from marine sediments under in vitro conditions. For this, biofilm quantification assays were performed using two methods, violet crystal and reduction of the XTT dye; In addition, the viability of the bacteria was evaluated by Live / Dead staining. Subsequently, the strains evaluated were identified using the 16S RNA marker. The results showed that the isolates belong to the genus Bacillus, all in different ranges were able to form filmsand strains B. safensis 64181 and Bacillus sp 64186 were selected which indicated greater production of this. Tests at different temperatures showed that for the strains selected the best temperature was 28 ° C. In addition, a mixed culture was carried out with these isolates, resultingin differences in the density of the biofilm and less changes in its metabolic activity in temperature variation experiments. From the results we can infer that bacterial consortiums can favor resistance to environmental variations in biofilms formed by bacteria of the genus Bacillus.

scholarly journals Evolutionary variations in the biofilm-associated protein BslA from the genus Bacillus

2016 ◽  
Author(s):  
Ryan J. Morris ◽  
Marieke Schor ◽  
Rachel M.C. Gillespie ◽  
Ana Sofia Ferreira ◽  
Keith M. Bromley ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Self Assembly ◽  
Molecular Basis ◽  
Bacillus Amyloliquefaciens ◽  
Bacillus Pumilus ◽  
Water Repellent ◽  
Biophysical Properties ◽  
Genus Bacillus ◽  
Degree Of Protection

AbstractBslA is a protein secreted by Bacillus subtilis which forms a hydrophobic film that coats the biofilm surface and renders it water-repellent. We have characterised three orthologues of BslA from Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus as well as a paralogue from B. subtilis called YweA. We find that the three orthologous proteins can substitute for BslA in B. subtilis and confer a degree of protection, whereas YweA cannot. The degree to which the proteins functionally substitute for native BslA correlates with their in vitro biophysical properties. Our results demonstrate the use of naturally-evolved variants to provide a framework for teasing out the molecular basis of interfacial self-assembly.

scholarly journals Two Novel Semisynthetic Lipoglycopeptides Active against Staphylococcus aureus Biofilms and Cells in Late Stationary Growth Phase

2021 ◽  
Vol 14 (11) ◽  
pp. 1182
Author(s):  
Vladimir Vimberg ◽  
Leona Zieglerova ◽  
Aninda Mazumdar ◽  
Zsolt Szűcs ◽  
Aniko Borbás ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Growth Phase ◽  
Stationary Growth Phase ◽  
Glycopeptide Antibiotics ◽  
Stationary Growth ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
New Antibiotics

The increase in antibiotic resistance among Gram-positive bacteria underscores the urgent need to develop new antibiotics. New antibiotics should target actively growing susceptible bacteria that are resistant to clinically accepted antibiotics including bacteria that are not growing or are protected in a biofilm environment. In this paper, we compare the in vitro activities of two new semisynthetic glycopeptide antibiotics, MA79 and ERJ390, with two clinically used glycopeptide antibiotics—vancomycin and teicoplanin. The new antibiotics effectively killed not only exponentially growing cells of Staphylococcus aureus, but also cells in the stationary growth phase and biofilm.

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