scholarly journals 175 Reduce Reuse ReRumen: Variability in nutritive value of paunch manure

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 55-56
Author(s):  
Taylor J Garcia ◽  
Jeffrey A Brady ◽  
Kimberly A Guay ◽  
James P Muir ◽  
William B Smith

Abstract Ruminant animals develop a diverse and complex microbial ecosystem for digesting fibrous feedstuffs, but this material represents a cost to the industry when bound for rendering at harvest. Our objective was to determine the feed value of paunch manure recovered from an abattoir. Twelve paunch samples were collected from slaughtered cattle at the Tarleton State University Meat Laboratory, Stephenville, TX. Samples were dried under forced air at 55°C to a constant weight and ground to pass through 2-mm screen. A subsample was ground to 1-mm and assayed for DM, CP, and sequential NDF and ADF. Physically effective fiber (peNDF) was calculated with 2 L of unground, dried at 55 ˚C, paunch material using the manually-operated Penn State Particle Separator. Paunch peNDF was determined by multiplying the physical effectiveness factor by the total NDF content of the sample. Samples (2-mm) were subject to batch culture in vitro digestibility assays for determination of digestibility coefficients. Data were analysed as a random effects model using PROC GLIMMIX in SAS. Neutral detergent fiber, ADF, ADL, CP, and peNDF values were 68.1, 39.9, 10.9, 15.0, and 38.7%, respectively. Contribution to variance of the individual animal for NDF, ADF, ADL, CP, and peNDF was 97.2, 97.9, 95.4, 19.1, and 97.5%, respectively. In vitro true digestibility (IVTD) and in vitro neutral detergent fiber digestibility (IVNDFD) was recorded as 46.2% and 21.6%, respectively. Contribution to variance of the animal for IVTD and IVNDFD was 99.05 and 98.49%, respectively. Data collected suggest paunch manure could provide valuable nutrients for livestock production, specifically fiber and protein. For full viability of application in a sustainable system, a centralized receiving and compositing system may be useful for development of a consistent product.

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 14-14
Author(s):  
Taylor J Garcia ◽  
Jeffrey A Brady ◽  
Kimberly A Guay ◽  
James P Muir ◽  
William B Smith

Abstract Ruminant animals develop a diverse and complex microbial ecosystem for digesting fibrous feedstuffs, but this material represents a cost to the industry when bound for rendering at harvest. Our objective was to determine the feed value of paunch manure recovered from an abattoir. Twelve paunch samples were collected from slaughtered cattle at the Tarleton State University Meat Laboratory, Stephenville, TX. Samples were dried under forced air at 55°C to a constant weight and ground to pass through 2-mm screen. A subsample was ground to 1-mm and assayed for DM, CP, and sequential NDF and ADF. Physically effective fiber (peNDF) was calculated with 2 L of unground, dried at 55 ˚C, paunch material using the manually-operated Penn State Particle Separator. Paunch peNDF was determined by multiplying the physical effectiveness factor by the total NDF content of the sample. Samples (2-mm) were subject to batch culture in vitro digestibility assays for determination of digestibility coefficients. Data were analysed as a random effects model using PROC GLIMMIX in SAS. Neutral detergent fiber, ADF, ADL, CP, and peNDF values were 68.1, 39.9, 10.9, 15.0, and 38.7%, respectively. Contribution to variance of the individual animal for NDF, ADF, ADL, CP, and peNDF was 97.2, 97.9, 95.4, 19.1, and 97.5%, respectively. In vitro true digestibility (IVTD) and in vitro neutral detergent fiber digestibility (IVNDFD) was recorded as 46.2% and 21.6%, respectively. Contribution to variance of the animal for IVTD and IVNDFD was 99.05 and 98.49%, respectively. Data collected suggest paunch manure could provide valuable nutrients for livestock production, specifically fiber and protein. For full viability of application in a sustainable system, a centralized receiving and compositing system may be useful for development of a consistent product.


Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3573
Author(s):  
Taylor Jacob Garcia ◽  
Nichole Marie Cherry ◽  
Kimberly Ann Guay ◽  
Jeffrey Alan Brady ◽  
James Pierre Muir ◽  
...  

Ruminants, which have multi-compartmented stomachs, are adapted to digest cellulosic materials, which constitute the primary expense on ranches and dairies. Industrial byproducts can be repurposed for livestock diets to decrease these costs. Therefore, finding alternative feedstuffs may benefit the economics of livestock production. The goal of this project was to evaluate the variation in nutritive value of ruminal waste as a potential feedstuff. Twelve paunch samples were collected from individual cattle across multiple harvest dates at the Tarleton State University Meat Laboratory, Stephenville, TX. Samples were dried and assayed for dry matter (DM), crude protein (CP), sequential neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL), and physically-effective fiber (peNDF). Samples were subjected to batch-culture in vitro digestibility assays for the determination of digestibility coefficients. Mean NDF, ADF, ADL, CP and peNDF concentrations were 681, 399, 109, 150, and 387 g kg−1 DM, respectively. Contribution to variance from sample for NDF, ADF, ADL, CP, and peNDF were 75.3, 41.9, 33.0, 51.2, and 71.3%, respectively. In vitro true digestibility (IVTD) and in vitro NDF digestibility (IVNDFD) were recorded as 462 and 216 g kg−1 DM, respectively. Contribution to variation of sample for IVTD and IVNDFD were 31.0 and 30.7%, respectively. Results indicate that rumen waste harvested from abattoirs may be useful for sustainable livestock production, while reducing environmental threats posed by disposal, but the viability of the product is highly dependent on the source animal. For full viability of application in a sustainable system, a centralized receiving and compositing system may be useful for developing a consistent product.


Sensors ◽  
2022 ◽  
Vol 22 (2) ◽  
pp. 658
Author(s):  
Matthew F. Digman ◽  
Jerry H. Cherney ◽  
Debbie J. R. Cherney

Advanced manufacturing techniques have enabled low-cost, on-chip spectrometers. Little research exists, however, on their performance relative to the state of technology systems. The present study compares the utility of a benchtop FOSS NIRSystems 6500 (FOSS) to a handheld NeoSpectra-Scanner (NEO) to develop models that predict the composition of dried and ground grass, and alfalfa forages. Mixed-species prediction models were developed for several forage constituents, and performance was assessed using an independent dataset. Prediction models developed with spectra from the FOSS instrument had a standard error of prediction (SEP, % DM) of 1.4, 1.8, 3.3, 1.0, 0.42, and 1.3, for neutral detergent fiber (NDF), true in vitro digestibility (IVTD), neutral detergent fiber digestibility (NDFD), acid detergent fiber (ADF), acid detergent lignin (ADL), and crude protein (CP), respectively. The R2P for these models ranged from 0.90 to 0.97. Models developed with the NEO resulted in an average increase in SEP of 0.14 and an average decrease in R2P of 0.002.


2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 66-67
Author(s):  
Taylor J Garcia ◽  
Jeffrey A Brady ◽  
Kimberly A Guay ◽  
James P Muir ◽  
William B Smith

Abstract Microbes and chemical constituents in the rumen fluid play an important role when re-establishing the rumen microbial population. Identification of a viable preservation method could have lasting implication of veterinary and research applications. Our objective was to determine the efficacy of preservation methods on rumen liquor. Twelve paunch samples were collected from slaughtered cattle at the Tarleton State University Meat Laboratory, Stephenville, TX. Rumen content was collected from different locations within the rumen, strained through eight layers of cheesecloth, and divided into five 500-mL aliquots. Aliquots were randomly allocated to one of five preservation methods (freezing or lyophilisation) and preservative (glycerol; yes or no). A fresh control was maintained from each sample. Three reference feeds and two roughage sources were used in the batch culture procedure. Reference feeds were dried to a constant weight at 55°C and ground to pass through a 2-mm screen. Ground samples were subjected to batch culture in vitro true digestibility (IVTD) assays using each of the four ruminal fluids (plus the control) for digestibility estimates. Original samples and incubated residues were assayed for DM, CP, NDF, and ADF. Data were analyzed in a 2 × 2 + 1 augmented factorial design using GLIMMIX procedure of SAS. There were no differences (P > 0.05) in IVTD when using protein or energy feeds; however, there were differences in IVTD for all treatments for fiber concentrates and forage reference feeds. Despite lower degradation of feeds when frozen or lyophilized rumen fluid was used, preserved rumen fluid may be a viable option in the absence of fresh inoculum for veterinary and research applications


2017 ◽  
Vol 26 (1) ◽  
pp. 47 ◽  
Author(s):  
Mahmoud F. Seleiman ◽  
Shaimaa Selim ◽  
Seija Jaakkola ◽  
Pirjo S.A. Mäkelä

Maize cultivation for silage could be a sustainable option in Boreal conditions, especially when combined with nutrient recycling. Effects of digestate (sludge from biogas of domestic origin) application in comparison with synthetic fertilizer and two maturity stages on chemical composition and in vitro digestibility of whole-crop maize were investigated. Starch, neutral detergent fiber, water soluble carbohydrate (WSC) and digestible organic matter (DOM) contents of maize did not differ in response to the two fertilizer treatments. However, starch, DOM and metabolizable energy of maize increased, while ash, crude protein and WSC contents decreased with increasing maize maturity. Heavy metals in maize fertilized with digestate remained low. The results indicate that whole-crop maize fertilized with digestate and harvested at 150 days after sowing is a promising feed and has good nutritive value, even in Boreal conditions.


2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 46-47
Author(s):  
Cody Davis ◽  
Russell C Carrell ◽  
Serena Thompson ◽  
Madison Cole ◽  
S Leanne L Dillard

Abstract In vitro digestibility (IVTD) is a useful tool for determining nutritive value of forages. The majority of equine forage digestion occurs in the cecum; however, many studies use fecal inoculum. The current study aimed to determine the optimal buffer:cecal fluid for batch culture fermentation and compare this method with fecal IVTD. Three horses were sourced from the Auburn University Veterinary School. Fecal material was collected via a rectal grab sample, placed in a plastic bag, and into a 39°C water bath. The cecal contents were collected using an abdominal incision within 5 min of termination. Contents were placed in a pre-warmed (39°C) thermos. Cecal fluid was strained and proportioned into 3 treatments (100:0, 75:25, and 50:50 cecal fluid:buffer). Fecal material was weighed and mixed with buffer to achieve a 10:90 by weight. Three samples of alfalfa, bermudagrass, and perennial peanut hay were randomly assigned to a fluid treatment and placed in the respective jar, digested for 48h, then underwent neutral detergent fiber analysis. The experiment was designed as a 4 × 3 factorial with horse considered random (n = 9). Statistical analysis was conducted using Proc Mixed (SAS Inst, Cary, NC) with α = 0.05. There was no effect of fluid treatment on IVTD (P = 0.12). However, a forage effect was observed (P < 0.001). Alfalfa and perennial peanut IVTD were not different (75.6%; P = 0.40), but greater (P < 0.001) than bermudagrass (48.1%). This study showed that no difference in cecal fluid and fecal material occurred when used as microbial inoculant sources for a Daisy-II batch fermentation for determination of equine IVTD.


2018 ◽  
Vol 22 (4) ◽  
pp. 179
Author(s):  
Wisri Puastuti ◽  
Dwi Yulistiani ◽  
Eko Handiwirawan

<p>Corn cob contains high fiber and lignin which causes low nutritive value. The objective of the study was to improve the digestibility of ammoniated corn cob (CC) by supplementation of molasses and branched-chain amino acid (BCAA: valine, leucine and isoleucine). CC was processed by addition 3% urea. The first stage of in vitro test was done with 4 levels of molases 0, 5, 10 and 15% of dry matter (DM) of CC. The experiment was carried out using complete randomized design with 4 treatments and 4 replications. The second stage was also in vitro study of supplementation of two levels each for valine = V, leucine = L and isoleucine = I namely 0.1 and 0.2% of DM of ammoniated CC. There were 8 treatments combination of the BCAA as follow: A = V0.1 L0.1 I0.1; E = V0.2 L0.1 I0.1; B = V0.1 L0.1 I0.2; F = V0.2 L0.1 I0.2; C = V0.1 L0.2 I0.1; G = V0.2 L0.2 I0.1; D = V0.1 L0.2 I0.2; H = V0.2 L0.2 I0.2 and 1 control (V0.0 L0.0 I0.0). The experiments were done using completely randomized design with 9 treatments and 3 replications. The results showed that treated CC with urea was able to increase protein content by 78% (increased from 3.34% to 5.95%) while neutral detergent fiber (NDF) decreased by 15.4%, acid detergent fiber (ADF) by 7.9% and lignin 16.7%. Addition of molasses in ammoniated CC increased DM digestibility (P &lt;0.05) by 7.5% (41.9 vs 43.51-46.26%) and NDF by 17.7% (38.41 vs 43.76 – 46.31%). Supplementation of BCAA resulted in the highest DM, OM and NDF digestibility (P &lt;0.05) in the treatment of A, C, D and G. Compare to treament I, the digestibility of DM, OM and NDF in G treatment increased by 31.4%, 27.5% and 36.5%, respectively and produced the highest total population of rumen bacterial of 12.4 x 109 colonies /ml. It can be concluded that the digestibility of ammoniated CC increased by the supplementation of 5% molases and BCAA combination consisted of 0.2% valine, 0.2% leucine and 0.1% isoleucine.</p><p> </p><p>Kata kunci: Tongkol jagung, amoniasi, molases, asam amino bercabang.</p>


2017 ◽  
Vol 18 (3) ◽  
pp. 417-429 ◽  
Author(s):  
Aline Cardoso OLIVEIRA ◽  
Rasmo GARCIA ◽  
Aureliano José Vieira PIRES ◽  
Hellenn Cardoso OLIVEIRA ◽  
Vitor Visintin Silva de ALMEIDA ◽  
...  

SUMMARY The experiment was developed to evaluate the effects of wilting and inclusion of cassava meal level on nutritive value, fermentative characteristics and losses of elephant grass silage. The 4 x 2 factorial scheme was adopted, with four repetitions, in a completely randomized design. The factors evaluated were wilting (without and with) and cassava meal level (0; 7.5; 15 and 22.5% of natural matter, DN). PVC silos with 50 cm height and 10 cm diameter were used for silages production, which were opened after 60 days. The wilting and cassava meal addition had increasing linear effect on DM content. Silages with cassava meal showed reduction of total nitrogen, ether extract, acid detergent insoluble nitrogen, neutral detergent fiber ash and protein free, acid detergent fiber, celluloses, hemicelluloses and lignin contents and increase of organic matter content, DM in vitro digestibility and total digestible nutrients. Relative to variables inherent to silages’ fermentative characteristics, pH presented quadratic behavior. Concerning to ammonia nitrogen, it was observed quadratic and decreasing linear behavior for silages without and with wilting, respectively. Wilting and cassava meal inclusion provided smaller effluent losses.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 154-155
Author(s):  
Taylor Burrell ◽  
Shelby L Wood ◽  
Nichole M Cherry ◽  
James P Muir ◽  
William B Smith

Abstract It is important for researchers, as progressive producers, to consider human enterprise wastes as one of the next steps in feedstuff investigation. In an effort to reuse a seemingly useless and disposable component of one of America’s most enjoyed aquatic delicacies, our objective was to determine the nutritive value of crawfish and shrimp shells in relation to ruminant nutrition. These discarded items were collected from local events and sorted into four different groups: crawfish heads, crawfish tails, shrimp shells, and shrimp tails. Crawfish and shrimp wastes were tested independent of each other. These groups were dried at 55°C for 72 h and ground to pass through a 2-mm screen. Samples were subjected to a batch-culture in vitro true digestibility (IVTD) assay. A separate set of samples (1 mm) were assayed for dry matter, neutral detergent fiber (NDF), and acid detergent fiber (ADF), and acid detergent lignin (ADL; inclusive of residual ash). Data were analyzed as a completely randomized design using the MIXED procedure of SAS. Crawfish heads were assayed to contain 26.2% NDF, 13.4% ADF, and 1.0% ADL, while crawfish tails contained 23.7% NDF, 16.3% ADF, and 1.1% ADL. Crawfish heads and tails did not differ in IVTD (P = 0.48) or in vitro NDF digestibility (IVNDFD; P = 0.13; 76.7 vs. 77.2% and 86.8 vs. 91.0%, respectively). Shrimp shells contained 36.1% NDF, 26.9% ADF, and 4.1% ADL, whereas shrimp tails contained 29.1% NDF, 17.9% ADF, and 0.7% ADL. Shrimp tails had greater (P &lt; 0.01) IVTD (74.2 vs. 66.4%) but lesser (P = 0.01) IVNDFD than shrimp shells (80.6 vs. 85.0%). Results are interpreted to mean that crustacean waste may represent a suitable prospective feedstuff for further evaluation.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 391-391
Author(s):  
Lauren P Bielamowicz ◽  
James P Muir ◽  
William B Smith

Abstract The use of by- or co-products in livestock production is not a novel concept. However, as adoption of a novel feed ingredient increases, the cost efficiency of its use generally decreases. Therefore, discovery of novel feed ingredients is a worthwhile academic pursuit. Our objective was to determine the storage stability of popcorn (Zea mays L.) in terms of gain or loss in nutritive value. Popcorn was collected on consecutive weekdays in two wk from a local cinema. Samples (n = 10) were subdivided into 9 aliquots and subjected to storage, uncovered, for 0, 1, 2, 3, 7, 35, 70, 105, and 140 d. Following the assigned storage time, samples were dried at 55°C to determine weight loss. Samples were dried at 55°C, ground to pass through a 2-mm screen, and subjected to a batch-culture in vitro assay for digestibility estimates [in vitro true digestibility (IVTD) and in vitro NDF digestibility (IVNDFD)]. Subsamples were ground to 1 mm assayed for neutral detergent fiber and acid detergent fiber. There was a linear increase (P &lt; 0.01) in weight following open storage which was attributed to absorption of ambient moisture. There was a linear decrease (P = 0.03) in NDF, with values decreasing from 20.2% at d 0 to 16.6% at d 140. However, there was no effect of storage on ADF (P = 0.29), IVTD (P = 0.38), or IVNDFD (P = 0.37). Results are interpreted to mean that waste popcorn is relatively shelf stable and may be a viable candidate as a feedstock for diet formulation.


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