PSXIII-4 Species feature of morphometric parameters in poultry semen
Abstract The aim of the research was to study the specific features of morphometric parameters in rooster and guinea fowl spermatozoa. Thirty ejaculate samples from each species were investigated. A macroscopic assessment of the ejaculates obtained was carried out by volume, color and consistency. Microscopic parameters were studied using computerized sperm quality analysis systems. Diff-Quick set was used for staining sperm. Visualization of fixed preparations was performed using a Nikon Eclipse Ni-U microscope and a Nikon DS-Qi2 camera. Measurement of morphometric parameters was carried out in the program NIS-Elements BR 4.30. Evaluation of sperm morphometry was carried out according to the following indicators: total length of spermatozoa, length of head and tail, perimeter and area, head shape in indexes of ellipticity and elongation. The SPSS v.15.0 package was used for statistical analysis. To assess the statistical differences in the distributions of spermatozoa, the χ2-test was used. ANOVA was conducted to determine the effect of species. Roosters surpassed guinea fowls in ejaculate volume by 83.3% (P < 0.001). Statistically significant differences were not established for sperm activity between species. The content of motile spermatozoa in the roosters ejaculate was 92.4 ± 5.3%, in guinea fowls - 88.7 ± 6.54%. The results of the morphometric parameters analysis showed that they have a specific feature. The total length of rooster spermatozoa was 90 ± 2.6 μm, which is 8 μm more than that in guinea fowl (P < 0.05). The length of the sperm head in roosters was 2 μm longer than that in guinea fowls (P < 0.05). The interspecific difference across the perimeters and width of the head was not statistically significant. Rooster ejaculates differed relative homogeneity compared to guinea fowl. The study results showed that morphometric parameters of poultry sperm have a specific feature. Supported by the RSF (16-16-04104).