81 Evaluation of feeding immunoglobulin-Y to Holstein feeder cattle for the control of liver abscesses & reduction of macrolide-resistant bacteria dissemination

Abstract Despite the regular use of macrolide-antibiotics in the diets of feedlot cattle, liver abscesses persist representing a financial burden to both pre- and post-mortem sectors of the beef industry. Alternative strategies, which simultaneously address liver infections and reduce the dissemination of antibiotic-resistant bacteria, are needed. An immunoglobulin-Y (IGY) product, developed to target Fusobacterium necrophorum andTrueperella pyogenes, was evaluated for the control of liver abscesses and its effect on macrolide-resistant Enterococcus growth from feces. Sixty-four Holstein steers (Initial BW= 384±13.5kg) consuming a finishing diet (90% concentrate) for 188 d (Final BW= 660±42 kg) were used in a completely randomized design where treatments included: TYL (Tylosin phosphate fed at 90 mg/hd/day; n = 32) or IGY (fed at 2.5g/hd/day; n = 32). Feed intake was recorded daily and BW was recorded every 28 d until slaughter. After 150 d on treatment fecal samples were randomly obtained from each treatment group four times (n = 40) and cultured on bile-esculine agar enriched with 8 μg/mL of erythromycin. Continuous variables of feedlot growth performance, carcass data and bacterial growth were analyzed using the MIXED procedure of SAS with the fixed effect of treatment and random effects of steer or sampling day, respectively. Ordinal carcass data was analyzed using the FREQ procedure of SAS observing the Cochran-Mantel-Haenszel value. Treatment did not affect DMI (P = 0.21), ADG (P = 0.50) or G:F (P = 0.36). Treatment neither affected hot carcass weight (P = 0.37) nor longissimus muscle area (P = 0.25). However, TYL resulted in greater back fat thickness than IGY (0.32 vs. 0.23 in.; P < 0.02) and consequently greater numerical yield grade (P < 0.02). Quality grade was not affected by treatment (P = 0.65). No treatment difference was observed for liver abscesses prevalence (TYL= 63% vs. IGY= 52%; P = 0.40) or severity (P = 0.23). There was a greater proportion of erythromycin-resistant Enterococcus growth for TYL than IGY (42.6 vs. 21.9%; P < 0.04). Specifically formulated IGY feed additives may be viable alternatives to antibiotics in the diets of feedlot cattle.

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82 Evaluation of feeding immunoglobulin-Y to Holstein feeder cattle for the control of liver abscesses & reduction of macrolide-resistant bacteria dissemination

Journal of Animal Science ◽

10.1093/jas/skz397.116 ◽

2020 ◽

Vol 98 (Supplement_2) ◽

pp. 50-51

Author(s):

Miranda K Stotz

Keyword(s):

Financial Burden ◽

Fusobacterium Necrophorum ◽

Feedlot Cattle ◽

Feed Additives ◽

Resistant Bacteria ◽

Continuous Variables ◽

Immunoglobulin Y ◽

Muscle Area ◽

Feeder Cattle ◽

Liver Abscesses

Abstract Despite the regular use of macrolide-antibiotics in the diets of feedlot cattle, liver abscesses persist representing a financial burden to both pre- and post-mortem sectors of the beef industry. Alternative strategies, which simultaneously address liver infections and reduce the dissemination of antibiotic-resistant bacteria, are needed. An immunoglobulin-Y (IGY) product, developed to target Fusobacterium necrophorum and Trueperella pyogenes, was evaluated for the control of liver abscesses and its effect on macrolide-resistant Enterococcus growth from feces. Sixty-four Holstein steers (Initial BW= 384±13.5kg) consuming a finishing diet (90% concentrate) for 188 days (Final BW= 660±42 kg) were used in a completely randomized design where treatments included: TYL (Tylosin phosphate fed at 90 mg/hd/day; n = 32) or IGY (fed at 2.5g/hd/day; n = 32).Feed intake was recorded daily and BW was recorded every 28 days until slaughter. After 150 d on treatment fecal samples were randomly obtained from each treatment group four times (n = 40) and cultured on bile-esculine agar enriched with 8 μg/mL of erythromycin. Continuous variables of feedlot growth performance, carcass data and bacterial growth were analyzed using the MIXED procedure of SAS with the fixed effect of treatment and random effects of steer or sampling day, respectively. Ordinal carcass data was analyzed using the FREQ procedure of SAS observing the Cochran-Mantel-Haenszel value. Treatment did not affect DMI (P = 0.21), ADG (P = 0.50) or G:F (P = 0.36). Treatment neither affected hot carcass weight (P = 0.37) nor longissimus muscle area (P = 0.25). However, TYL resulted in greater back fat thickness than IGY (0.32 vs. 0.23 in.; P < 0.02) and consequently greater numerical yield grade (P < 0.02). Quality grade was not affected by treatment (P = 0.65). No treatment difference was observed for liver abscesses prevalence (TYL= 63% vs. IGY= 52%; P = 0.40) or severity (P = 0.23). There was a greater proportion of erythromycin-resistant Enterococcus growth for TYL than IGY (42.6 vs. 21.9%; P < 0.04). Specifically formulated IGY feed additives may be viable alternatives to antibiotics in the diets of feedlot cattle.

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83 Evaluation of feeding immunoglobulin-Y to Holstein feeder cattle for the control of liver abscesses & reduction of macrolide-resistant bacteria dissemination

Journal of Animal Science ◽

10.1093/jas/skz397.022 ◽

2020 ◽

Vol 98 (Supplement_2) ◽

pp. 10-10

Author(s):

Miranda K Stotz ◽

Diego Casas Murillo ◽

Darren D Henry ◽

Whitney L Crossland

Keyword(s):

Financial Burden ◽

Feedlot Cattle ◽

Feed Additives ◽

Experimental Unit ◽

Resistant Bacteria ◽

Continuous Variables ◽

Immunoglobulin Y ◽

Muscle Area ◽

Feeder Cattle ◽

Liver Abscesses

Abstract Despite the regular use of macrolide-antibiotics in the diets of feedlot cattle, liver abscesses persist representing a financial burden to both pre- and post-mortem sectors of the beef industry. Alternative strategies, which simultaneously address liver infections and reduce the dissemination of antibiotic-resistant bacteria, are needed. An immunoglobulin-Y (IGY) product, developed to target Fusobacterium necrophorum and Trueperella pyogenes, was evaluated for the control of liver abscesses and its effect on macrolide-resistant Enterococcus growth from feces. Sixty-four Holstein steers (Initial BW= 384± 13.5kg) consuming a finishing diet (90% concentrate) for 188 days (Final BW= 660± 42 kg) were used in a completely randomized design where steer was experimental unit and treatments included: TYL (Tylosin phosphate fed at 90mg/hd/day; n = 32) or IGY (fed at 2.5g/hd/day; n= 32). Feed intake was recorded daily and BW was recorded every 28 days until slaughter. After 150 d on treatment fecal samples were randomly obtained from each treatment group four times (n = 40) and cultured on bile-esculine agar enriched with 8 μg/mL of erythromycin. Continuous variables of feedlot growth performance, carcass data and bacterial growth were analyzed using the MIXED procedure of SAS with the fixed effect of treatment and random effects of steer or sampling day, respectively. Ordinal carcass data were analyzed using the FREQ procedure of SAS observing the Cochran-Mantel-Haenszel value. Treatment did not affect DMI (P= 0.21), ADG (P = 0.50) or G:F (P = 0.36). Treatment neither affected hot carcass weight (P = 0.37) nor longissimus muscle area (P = 0.25). However, TYL resulted in greater back fat thickness than IGY (0.32 vs. 0.23 in.; P < 0.02) and consequently greater numerical yield grade (P < 0.02). Quality grade was not affected by treatment (P = 0.65). No treatment difference was observed for liver abscesses prevalence (TYL=63% vs. IGY=52%; P = 0.40) or severity (P = 0.23). There was a greater proportion of erythromycin-resistant Enterococcus growth for TYL than IGY (42.6 vs. 21.9%; P < 0.04). Specifically formulated IGY feed additives may be viable alternatives to antibiotics in feedlot cattle diets.

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Nutritionally related disorders affecting feedlot cattle

Canadian Journal of Animal Science ◽

10.4141/a02-061 ◽

2003 ◽

Vol 83 (1) ◽

pp. 13-20 ◽

Cited By ~ 32

Author(s):

M. L. Galyean ◽

J. D. Rivera

Keyword(s):

Environmental Factors ◽

Feed Intake ◽

Metabolic Disorders ◽

Microbial Population ◽

Feedlot Cattle ◽

Feed Additives ◽

Nutritional Management ◽

Direct Effects ◽

Liver Abscesses ◽

Digestive Disorders

Digestive disorders account for approximately 25 to 33% of deaths in feedlot cattle and likely contribute to decreased performance and efficiency of production. A variety of nutritional, management, genetic, behavioural, and environmental factors seem to be involved in the development of metabolic disorders in feedlot cattle. Excessive production of acid in the rumen is often either the cause of or a significant contributing factor to metabolic and nutritional disorders, including acute or sub-acute (chronic) acidosis, liver abscesses, and feedlot bloat. Decreasing the percentage of highly fermentable concentrates in feedlot diets by increasing roughage level or limiting feed intake should decrease the incidence of these disorders, but this approach is usually not economically feasible. Careful feed bunk management is often thought to be important for decreasing the incidence of nutritionally related disorders, but research to support its importance is limited and conflicting. Certain feed additives like ionophores seem to be effective for decreasing the incidence of acidosis and feedlot bloat, presumably through decreased total feed intake, smaller and more frequent meals, and direct effects on the ruminal microbial population. The incidence of liver abscesses can be decreased by the feeding of various antibiotics. Polioencephalomalacia (PEM) can result from several causes, including thiamine deficiency; however, recent data suggests that overproduction of H2S in the rumen is an important cause of PEM when intakes (feed and water) of sulfur are high in feedlot cattle. Key words: Beef cattle, acidosis, bloat, liver abscesses, polioencephalomalacia

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PSVII-40 Late-Breaking Abstract: Ultrasonography and bacterial community of naturally occurring liver abscesses and non-abscessed livers in finishing Holstein steers

Journal of Animal Science ◽

10.1093/jas/skaa278.603 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 339-340

Author(s):

Miranda K Stotz ◽

Whitney L Crossland

Keyword(s):

Financial Burden ◽

Feedlot Cattle ◽

Ultrasound Images ◽

Post Mortem ◽

Beef Industry ◽

Feedlot Performance ◽

Finishing Cattle ◽

Liver Abscesses ◽

Rdna Sequencing ◽

Severity Scores

Abstract Liver abscesses persist in feedlot cattle representing a financial burden to both pre- and post-mortem sectors of the beef industry. Pre- and Post-mortem strategies are needed to understand onset, duration, and resolution of abscesses, etiology, and fully quantify their impact on performance. Holstein steers (n = 56; Initial BW=384±13.5 kg) consuming a finishing diet (90% concentrate) for 188 d (Final BW=660±42 kg) were used for live ultrasonography of the liver. Feed intake was recorded daily and BW and liver ultrasound images recorded every 28 d. At harvest, livers were graded and imaged to compare with ultrasound images. Tissue was sampled from both abscessed and non-abscessed livers and 16s rDNA sequencing performed. Steers ranked within feedlot performance variables for 188 days on feed (DOF) and the final 48 DOF were compared with post-mortem liver score using the FREQ procedure of SAS. A contingency table for abscess detection by ultrasound versus harvest was used to evaluate ultrasound accuracy. Abscess prevalence of Holstein steers was 60.7%. No relationship was detected between liver severity score and DMI, ADG and G:F calculated from the total days on feed (P ≥ 0.394). However, in the last 48 DOF, steers with non-abscessed or resolved liver abscesses tended to have greater ADG values (P = 0.099) and G:F (P = 0.049) than steers with mild and severely abscessed livers. Final ultrasound identified 14 of 23 abscessed livers. Abscesses not detected by ultrasound were inactive scars/adhesions, located on the visceral side or covered by lung or intestinal tissue. Non-abscessed and scarred livers had greater OTU richness and evenness while severely abscessed livers had greater dominance (P < 0.001). Beta-diversity indicated community separation of tissue from different severity scores. All 56 liver samples contained F. necrophorum and Bacteroides spp. DNA; abscessed or not. The true prevalence and duration of liver abscesses in finishing cattle requires greater pre-mortem surveillance.

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Evaluation of Immunoglobulin-Y in Place of Tylosin Phosphate in the Diets Fed to Holstein Steers and Preliminary Analysis of Liver Abscess Duration on Animal Growth Performance

Translational Animal Science ◽

10.1093/tas/txaa225 ◽

2020 ◽

Author(s):

Miranda K Stotz ◽

Darren D Henry ◽

Whitney L Crossland

Keyword(s):

Growth Performance ◽

Liver Abscess ◽

Financial Burden ◽

Average Daily Gain ◽

Carcass Characteristics ◽

Immunoglobulin Y ◽

Liver Abscesses ◽

Performance Reduction ◽

Animal Growth ◽

The Impact

Abstract Despite the regular use of feed-grade macrolide-antibiotics, bovine liver abscesses persist, representing a financial burden to pre- and post-mortem sectors of the beef industry. An immunoglobulin-Y (IGY) additive developed to target Fusobacterium necrophorum and Trueperella pyogenes, was evaluated for the control of liver abscesses. Research is needed for the impact of liver abscess severity as well as abscess duration on steer performance and carcass characteristics. Holstein steers (n=64; initial body weight (BW)=372.5±2.41 kg) consuming a finishing diet for 188 d were used in a completely randomized design where treatments included: TYL (tylosin phosphate 90 mg/day; n=32) or IGY (2.5 g/day; n=32) and steer was the experimental unit. Feed intake was recorded daily while BW and liver ultrasound outcome (normal or abnormal) was recorded every 28 d until slaughter to estimate duration of abscess presence (DUR). Continuous variables of animal growth performance and carcass characteristics were analyzed using the MIXED procedure of SAS. Categorical quality grade and liver data were analyzed using the GLIMMIX procedure of SAS. Treatment did not affect live or carcass-adjusted growth performance (P≥0.131). However, steers fed TYL had greater (P=0.042) empty body fat (EBF) % and a greater proportion of carcasses grading premium choice than steers fed IGY (P=0.030). Treatment did not affect prevalence of abscessed livers, abscess severity or estimated abscess duration (P≥0.213) but datasets with greater experimental units are needed to substantiate this outcome. Increasing abscess severity tended (P≤0.10) to linearly reduce carcass-adjusted gain to feed (G:F), fat thickness, and EBF. Carcass dressing % was only affected by severe (A+ and A+AD) abscess scores (P=0.010). Carcass-adjusted final BW, average daily gain, G:F, and hot carcass weight was decreased only when the estimated DUR was ≥140 d (P≤0.05). Carcass dressing %, however, was linearly affected by estimated liver abscess DUR (P≤ 0.005), regardless of abscess severity. Preliminary evidence suggests that measuring the duration of liver abscess affliction during the feeding period may also give insight to the degree of performance reduction.

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Comparison of Fusobacterium necrophorum isolates from liver abscesses, ruminal walls, and ruminal contents of feedlot cattle

Kansas Agricultural Experiment Station Research Reports ◽

10.4148/2378-5977.1892 ◽

1998 ◽

pp. 47-49

Author(s):

Sanjeev K. Narayanan ◽

O. Okwumabua ◽

J. Staats ◽

Richard D. Oberst ◽

Tiruvoor G. Nagaraja ◽

...

Keyword(s):

Fusobacterium Necrophorum ◽

Feedlot Cattle ◽

Liver Abscesses

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Bacterial community analysis of purulent material from liver abscesses of crossbred cattle and Holstein steers fed finishing diets with or without tylosin

Journal of Animal Science ◽

10.1093/jas/skab076 ◽

2021 ◽

Vol 99 (4) ◽

Author(s):

Raghavendra G Amachawadi ◽

Wesley A Tom ◽

Michael P Hays ◽

Samodha C Fernando ◽

Philip R Hardwidge ◽

...

Keyword(s):

Bacterial Community ◽

Community Composition ◽

Bacterial Community Composition ◽

Feedlot Cattle ◽

Crossbred Cattle ◽

Sequence Analyses ◽

Gram Negative ◽

Treatment Groups ◽

Finishing Diets ◽

Liver Abscesses

Abstract Liver abscesses in feedlot cattle are polymicrobial infections. Culture-based studies have identified Fusobacterium necrophorum as the primary causative agent, but a number of other bacterial species are frequently isolated. The incidence of liver abscesses is highly variable and is affected by a number of factors, including cattle type. Holstein steers raised for beef production have a higher incidence than crossbred feedlot cattle. Tylosin is the commonly used antimicrobial feed additive to reduce the incidence of liver abscesses. The objective of this study was to utilize 16S ribosomal RNA amplicon sequence analyses to analyze the bacterial community composition of purulent material of liver abscesses of crossbred cattle (n = 24) and Holstein steers (n = 24), each fed finishing diet with or without tylosin. DNA was extracted and the V3 and V4 regions of the 16S rRNA gene were amplified, sequenced, and analyzed. The minimum, mean, and maximum sequence reads per sample were 996, 177,070, and 877,770, respectively, across all the liver abscess samples. Sequence analyses identified 5 phyla, 14 families, 98 genera, and 102 amplicon sequence variants (ASV) in the 4 treatment groups. The dominant phyla identified were Fusobacteria (52% of total reads) and Proteobacteria (33%). Of the top 25 genera identified, 17 genera were Gram negative and 8 were Gram positive. The top 3 genera, which accounted for 75% of the total reads, in the order of abundance, were Fusobacterium, Pseudomonas, and Bacteroides. The relative abundance, expressed as percent of total reads, of phyla, family, and genera did not differ (P > 0.05) between the 4 treatment groups. Generic richness and evenness, determined by Shannon–Weiner and Simpson’s diversity indices, respectively, did not differ between the groups. The UniFrac distance matrices data revealed no clustering of the ASV indicating variance between the samples within each treatment group. Co-occurrence network analysis at the genus level indicated a strong association of Fusobacterium with 15 other genera, and not all of them have been previously isolated from liver abscesses. In conclusion, the culture-independent method identified the bacterial composition of liver abscesses as predominantly Gram negative and Fusobacterium as the dominant genus, followed by Pseudomonas. The bacterial community composition did not differ between crossbred and Holstein steers fed finishing diets with or without tylosin.

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Impact of a phytogenic feed additive on growth performance, feed intake, and carcass traits of finishing steers

Translational Animal Science ◽

10.1093/tas/txz109 ◽

2019 ◽

Vol 3 (4) ◽

pp. 1162-1172

Author(s):

Tassilo Brand ◽

Martin Hünerberg ◽

Tim A McAllister ◽

Maolong He ◽

Atef M Saleem ◽

...

Keyword(s):

Growth Performance ◽

Feed Intake ◽

Dry Matter ◽

Average Daily Gain ◽

Carcass Traits ◽

Feed Additive ◽

Muscle Area ◽

Mineral Supplement ◽

Liver Abscesses ◽

Finishing Steers

Abstact: The purpose of this study was to evaluate the effect of a phytogenic feed additive (Digestarom [DA]; Biomin, Getzersdorf, Austria) on growth performance, feed intake, carcass traits, fatty acid composition, and liver abscesses of finishing steers. One hundred twenty Angus × Charolais crossbred steers (488 ± 26.5 kg) were used in a 110-d feeding experiment. Steers were blocked by weight and randomly assigned to 12 pens with 10 steers per pen. Each pen was allocated to one of three diets. Each diet contained 86.5% barley, 10.0% barley silage, and 3.5% vitamin and mineral supplement on a dry matter (DM) basis. The diets contained 0, 0.05, and 0.1 g DA/kg complete diet (DM basis), to achieve average daily DA intakes of 0 (control), 0.5 (LowDA), and 1.0 g (HighDA) per steer. Diets were prepared once daily and provided ad libitum. Two pens per treatment were equipped to record individual feed intake behavior. Steers were weighed every 28 d and carcass traits and liver scores were recorded at slaughter. Dry matter intake (average: 9.34 kg/d) did not differ (P > 0.05) among diets. Average daily gain tended to increase linearly as DA increased (control: 1.82; LowDA: 1.87; and HighDA: 1.95 kg/d; P < 0.09), but gain:feed ratio was not affected. Supplementation of DA affected longissimus muscle area quadratically (P = 0.05) with the largest area observed for LowDA. However, dressing percentage decreased linearly in response to increasing level of DA (P < 0.01). Total abscessed livers were not affected, whereas proportion of severe liver abscesses was numerically lower with DA (30.8% and 42.5% for LowDA and HighDA) compared to the control (50%).

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Antimicrobial Susceptibility of Lactic Acid Bacteria Strains of Potential Use as Feed Additives - The Basic Safety and Usefulness Criterion

Frontiers in Veterinary Science ◽

10.3389/fvets.2021.687071 ◽

2021 ◽

Vol 8 ◽

Author(s):

Ilona Stefańska ◽

Ewelina Kwiecień ◽

Katarzyna Jóźwiak-Piasecka ◽

Monika Garbowska ◽

Marian Binek ◽

...

Keyword(s):

Antibiotic Resistance ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Resistance Genes ◽

Pathogenic Bacteria ◽

Feed Additives ◽

Health Concern ◽

Resistant Bacteria ◽

Safety Criterion ◽

Phenotypic Resistance

The spread of resistance to antibiotics is a major health concern worldwide due to the increasing rate of isolation of multidrug resistant pathogens hampering the treatment of infections. The food chain has been recognized as one of the key routes of antibiotic resistant bacteria transmission between animals and humans. Considering that lactic acid bacteria (LAB) could act as a reservoir of transferable antibiotic resistance genes, LAB strains intended to be used as feed additives should be monitored for their safety. Sixty-five LAB strains which might be potentially used as probiotic feed additives or silage inoculants, were assessed for susceptibility to eight clinically relevant antimicrobials by a minimum inhibitory concentration determination. Among antimicrobial resistant strains, a prevalence of selected genes associated with the acquired resistance was investigated. Nineteen LAB strains displayed phenotypic resistance to one antibiotic, and 15 strains were resistant to more than one of the tested antibiotics. The resistance to aminoglycosides and tetracyclines were the most prevalent and were found in 37 and 26% of the studied strains, respectively. Phenotypic resistance to other antimicrobials was found in single strains. Determinants related to resistance phenotypes were detected in 15 strains as follows, the aph(3″)-IIIa gene in 9 strains, the lnu(A) gene in three strains, the str(A)-str(B), erm(B), msr(C), and tet(M) genes in two strains and the tet(K) gene in one strain. The nucleotide sequences of the detected genes revealed homology to the sequences of the transmissible resistance genes found in lactic acid bacteria as well as pathogenic bacteria. Our study highlights that LAB may be a reservoir of antimicrobial resistance determinants, thus, the first and key step in considering the usefulness of LAB strains as feed additives should be an assessment of their antibiotic resistance. This safety criterion should always precede more complex studies, such as an assessment of adaptability of a strain or its beneficial effect on a host. These results would help in the selection of the best LAB strains for use as feed additives. Importantly, presented data can be useful for revising the current microbiological cut-off values within the genus Lactobacillus and Pediococcus.

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