P0161TO EVALUATE THE TH PROFILE (TH1/TH2/TH17 ) AMONG GESTATIONAL DIABETES AND NORMAL PREGNANCY

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Anupma Kaul ◽  
Amita Pandey ◽  
Vikas Aggrwal
Keyword(s):  
Gestational Diabetes ◽  
High Sensitivity ◽  
Normal Pregnancy ◽  
Treg Cells ◽  
System Response ◽  
Control Group ◽  
Low Grade ◽  
The Third ◽  
Uncomplicated Pregnancy ◽  
Intracellular Cytokines

Abstract Background and Aims Gestational diabetes mellitus (GDM) is a common pregnancy complication, characterized by insulin resistance and low-grade systemic inflammation with a pro-inflammatory immune system response. Our objective was to study the peripheral Th1, Th2, Th17 and Treg response in GDM compared to normal pregnancy. Method Th1, Th2, Th17 and Treg subsets was determined by flow cytometry based on staining for specific intracellular cytokines levels and their CRP levels were also assessed.. The health status of all offspring was also assessed 6 months post-delivery. Results Α total of 32 adult pregnant women were enrolled into a GDM (n=18) and Control (n=14) group. At the third trimester of pregnancy, the GDM group had a higher proportion of Th2, Th17 and Treg cells compared to control. Contrary to the control group, the GDM group exhibited no significant change in the Th1/Th2/Th17/Treg profile postpartum. A higher circulating CRP were noted in the GDM group compared to controls. At the 6-month post-delivery assessment, 23.6% of the offspring from the GDM group were found to have developed atopic dermatitis or food allergy compared to none from the control group. Conclusion Compared to an uncomplicated pregnancy, GDM exhibits a significantly different peripheral T-cell profile at the third pregnancy trimester characterized by higher proportion of Th2, Th17 and Treg cells which persist six months post-delivery, while the increased high sensitivity CRP (hsCRP) levels stressed the low-grade inflammatory profile of this disease

A COMPARATIVE STUDY OF LEVELS OF HIGH SENSITIVITY CREACTIVE PROTEIN (hs-CRP) IN DIABETIC AND NON- DIABETIC POPULATION OF CENTRAL INDIA

2021 ◽  
pp. 31-32
Author(s):  
Parag Gupta ◽  
Lal Pranay Singh
Keyword(s):  
Diabetes Mellitus ◽  
Central India ◽  
High Sensitivity ◽  
Control Group ◽  
Low Grade ◽  
Medical College ◽  
Diabetic Population ◽  
Hs Crp ◽  
And Control

Background: Diabetes mellitus is the most common metabolic disorder characterized by metabolic abnormalities and long term complications. It is characterized by hyperglycemia resulting from defect in insulin secretion and in its action. It has become a leading cause of morbidity and mortality world over. hs- CRP is a marker of low-grade inammation and it is raised in patients with type 2 DM. The present study was undertaken with the objective of studying the relation of High Sensitivity CRP (hs-CRP) in patients of type 2 Diabetes Mellitus and in non-diseased population. Methods: This was a comparative observational study, conducted at Diabetic clinic, Index Medical College, Indore during August 2014 to July 2015. Cases and control were selected as per the inclusion criteria. Statistical analysis was done using SPSS v20. Mean age of the group was 48years. The mean hs CRP in the diabe Results: tic group was 0.45 compared with 0.35, in the control group. hs CRP levels are directly related to insulin resistance and is Conclusion: highly associated with diabetic population.

scholarly journals Nesfatin-1 and Vaspin as Potential Novel Biomarkers for the Prediction and Early Diagnosis of Gestational Diabetes Mellitus

2019 ◽  
Vol 20 (1) ◽  
pp. 159 ◽  
Author(s):  
Radzisław Mierzyński ◽  
Elżbieta Poniedziałek-Czajkowska ◽  
Dominik Dłuski ◽  
Jolanta Patro-Małysza ◽  
Żaneta Kimber-Trojnar ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Early Diagnosis ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Medical Complication ◽  
Uncomplicated Pregnancy ◽  
Novel Biomarkers

Gestational diabetes mellitus (GDM) is considered to be one of the most frequent medical complication observed among pregnant women. The role of adipokines in the pathogenesis of GDM remains strictly unknown. Different adipokines have been studied throughout gestation, and they have been proposed as biomarkers of GDM and other pregnancy-related complications; however, there is no biomarker reported for GDM screening at present. The aim of this study was to evaluate serum nesfatin-1 and vaspin levels in GDM and non-GDM women, to characterize the correlation between these adipokines, and to assess the potential role of circulating adipokines in the prediction of risk of gestational diabetes mellitus. Serum concentrations of nesfatin-1 and vaspin were measured in 153 women with GDM, and in 84 patients with uncomplicated pregnancy by enzyme-linked immunosorbent assay (ELISA) kits, according to the manufacturer’s instructions. Circulating levels of nesfatin-1 and vaspin were significantly lower in the GDM group than in the control group. Nesfatin-1 levels were negatively correlated with vaspin levels. The results of this study point out the possible role of nesfatin-1 and vaspin as potential novel biomarkers for the prediction and early diagnosis of GDM. Further studies are necessary to evaluate the influence of nesfatin-1 and vaspin on glucose metabolism in the early stages of GDM.

Evaluation of oxidative stress and antioxidant activity in women with moderate and severe preeclampsia in the third trimester of pregnancy and their newborns

2020 ◽  
Vol 65 (12) ◽  
pp. 733-737
Author(s):  
Irina Gennadievna Popova ◽  
O. G. Sitnikova ◽  
S. B. Nazarov ◽  
R. I. Sadov ◽  
I. A. Panova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radical Oxidation ◽  
Control Group ◽  
Third Trimester ◽  
Radical Oxidation ◽  
The Third ◽  
Uncomplicated Pregnancy ◽  
Group 2 ◽  
Group 1

We examined 66 women who were 22-40 weeks pregnant and their newborns. Of these, 15 women with moderate PE were in group 1, 22 women with severe PE were in group 2, and 55 women with uncomplicated pregnancy without hypertensive disorders were in the control group. Blood was taken from women when they were admitted to the clinic, and newborns ‘ blood was taken for 3-5 days of life. Free radical oxidation and antioxidant activity were evaluated by induced chemiluminescence. It was found that in patients with severe and moderate preeclampsia, the development of oxidative stress is accompanied by a weakening of antioxidant activity. In newborns born to mothers with preeclampsia, oxidative stress is accompanied by a compensatory increase in antioxidant activity.

Evaluation of the psychological and biological changes of patients diagnosed with benign and malignant breast tumors

2012 ◽  
Vol 27 (4) ◽  
pp. 322-330 ◽  
Author(s):  
Lan-Jun Bai ◽  
Qing Liu ◽  
Min Wang ◽  
Wings TY Loo ◽  
Rebecca Wy Cheng ◽  
...  
Keyword(s):  
Benign Tumor ◽  
Malignant Tumors ◽  
Chinese Women ◽  
High Sensitivity ◽  
Psychological Trauma ◽  
Breast Tumors ◽  
Serum Levels ◽  
Control Group ◽  
Low Grade ◽  
Malignant Breast

Purpose Posttraumatic stress disorder (PTSD) is a severe anxiety disorder developed by exposure to any incident or circumstance that results in psychological trauma. In this study we compared the psychological and physiological changes between patients with malignant and benign breast tumors. Methods We selected 150 Chinese women with a breast mass, aged 20 to 45 years, from the Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital between 2009 and 2011 for this study; 30 healthy participants were enrolled into the control group. All subjects were examined and had their tumor mass aspirated for diagnosis. Equal numbers of patients with benign and malignant tumors were recruited. Patients with malignant tumors presented with low grade, minimal tumor invasion and non-involved lymph nodes. Questionnaires regarding anxiety, depression and PTSD were conducted 2 hours before getting the diagnostic result and 1 month after the diagnosis. Serum levels of IL-6, TNF-, cortisol and high-sensitivity C-reactive protein before and after diagnosis were investigated and compared. The number of occurrences of oral ulcerative mucositis was also recorded. Results All patients experienced a certain degree of anxiety and their biomarkers were elevated compared with the normal reference range before the pathological report was disclosed. However, 1 month after the operation, the benign tumor group showed significantly lower levels of biomarkers and anxiety scores than patients with a malignant breast tumor. The results were consistent throughout 12 months of study. Conclusion Study subjects with a benign tumor returned to their normal condition after being diagnosed, while patients with a malignant tumor suffered from a certain degree of PTSD or depression.

scholarly journals Sex Specific Expression of Interleukin 7, 8 and 15 in Placentas of Women with Gestational Diabetes

2020 ◽  
Vol 21 (21) ◽  
pp. 8026
Author(s):  
Simon Keckstein ◽  
Sophia Pritz ◽  
Niklas Amann ◽  
Sarah Meister ◽  
Susanne Beyer ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Gestational Diabetes ◽  
Placental Tissue ◽  
Extravillous Trophoblast ◽  
Control Group ◽  
Low Grade ◽  
Maternal Complications ◽  
Specific Expression ◽  
Interleukin 7 ◽  
Low Grade Inflammation

Gestational diabetes mellitus (GDM) is known to increase the risk for feto-maternal complications during pregnancy. A state of low-grade inflammation, with elevated levels of proinflammatory molecules, similar to patients with obesity or diabetes mellitus type 2 has also been partly described in GDM. The placenta, as unique interface between mother and fetus, is not only passively affected by changes in one of these organisms, but also acts as a modulator by expressing hormones and cytokines. This study aimed to investigate the expression of the proinflammatory cytokines Interleukin (IL) 7, 8 and 15 in GDM in placental tissue. A total number of 80 placentas were included (40 GDM/40 control group). The expression of IL-7, 8 and 15 was investigated in extravillous trophoblast (EVT) and syncytiotrophoblast (SCT) by immunohistochemistry and immunofluorescence double staining. The immunohistochemical staining was evaluated with the semiquanitfied immunoreactive score (IRS). While the expression IL-15 was significantly upregulated in EVTs of women with GDM. The expression of IL-8 was significantly decreased in EVT of the GDM group. Furthermore, significant fetal sex specific differences were detectable in all three cytokines. Our findings suggest an involvement of the investigated cytokines in the maintenance of a state of chronic low-grade inflammation on placental level in patients suffering from GDM.

scholarly journals Correlation of maternal serum fetuin/alpha2-HS-glycoprotein concentration with maternal insulin resistance and anthropometric parameters of neonates in normal pregnancy and gestational diabetes

2002 ◽  
pp. 243-248 ◽  
Author(s):  
L Kalabay ◽  
K Cseh ◽  
A Pajor ◽  
E Baranyi ◽  
GM Csakany ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Gestational Diabetes ◽  
Pregnant Women ◽  
Normal Pregnancy ◽  
Tnf Alpha ◽  
Control Group ◽  
Anthropometric Parameters ◽  
Necrosis Factor Alpha ◽  
C Peptide ◽  
Glucose Ratio

OBJECTIVE: Human fetuin/alpha(2)-HS-glycoprotein (AHSG) is a 49 kDa serum and tissue protein which is a natural inhibitor of insulin receptor signaling. We investigated serum AHSG levels during pregnancy and whether the protein is involved in insulin resistance observed in healthy pregnant women and patients with gestational diabetes. DESIGN: One hundred and four healthy pregnant women and 23 of their neonates, 30 patients with gestational diabetes and their neonates and 30 healthy age-matched non-pregnant females as a control group were investigated in a case-control cross-sectional study. METHODS: Serum AHSG was determined by radial immunodiffusion. RESULTS: We observed an increase of serum AHSG concentration in the second and third trimesters. Gestational diabetes patients had significantly higher AHSG levels than healthy pregnant women and non-pregnant controls. There was a highly significant positive correlation between serum AHSG concentration and indirect parameters of insulin resistance, i.e. tumor necrosis factor-alpha (TNF-alpha), leptin, C-peptide and C-peptide/blood glucose ratio. There was also a negative correlation between maternal AHSG, TNF-alpha, leptin levels and head circumference, body length and body weight of newborns. CONCLUSION: AHSG, TNF-alpha and leptin may contribute to insulin resistance during normal pregnancy and gestational diabetes. AHSG along with these cytokines may also negatively regulate neonatal skeletal development.

Hematologic parameters as biomarkers for antihistamine and omalizumab resistance in chronic spontaneous urticaria

2021 ◽  
Vol 42 (1) ◽  
pp. e17-e24 ◽  
Author(s):  
Eli Magen ◽  
Dan-Andrei Waitman ◽  
Natan R. Kahan
Keyword(s):  
High Sensitivity ◽  
Study Groups ◽  
Chronic Spontaneous Urticaria ◽  
Control Group ◽  
Low Grade ◽  
Reactive Protein ◽  
Record Data ◽  
The Mean ◽  
Low Grade Inflammation

Background: Biomarkers of resistance to H1-antihistamines (AH) and omalizumab in chronic spontaneous urticaria (CSU) are still a matter of debate. Objective: To identify clinical and laboratory attributes of the patient that may be predictive of AH and omalizumab resistance in CSU. Methods: We conducted a retrospective observational study by using the electronic patient record data base of patients with CSU and of sex- and age-matched controls. Patients with CSU were divided into three study groups: the CSU group, patients who responded to AHs; the antihistamine-resistant CSU (AH-CSU) group, patients refractory to a fourfold AH dose; and the control group, composed of a random sample of age- and sex-matched subjects, with a case-control ratio of 1:2. The patients in the AH-CSU group treated with omalizumab were compared according to the response or resistance to omalizumab. Results: A total of 106 subjects in the AH-CSU group, 483 in the CSU group, and 1198 in the control group were compared. Both AH-CSU (112.7 ± 43.1 kU/mL) and CSU (129.5 ± 52.4 kU/mL) groups were associated with higher plasma total IgE levels than control group (103.2 ± 49.5 kU/mL; p < 0.001). The AH-CSU group was characterized by a higher plasma high-sensitivity C-reactive protein level (6.4 ± 3.7 mg/L) than the CSU group (4.3 ± 1.4 mg/L; p < 0.001) and the control group (3.1 ± 1.8 mg/L; p < 0.001). The AH-CSU and CSU groups were characterized by a lower mean ± standard deviation basophil counts (0.18 ± 0.16 cells ×109/L and 0.19 ± 0.11 cells ×109/L, respectively) than the control group (0.22 ± 0.09 cells ×109/L; p < 0.001). The mean platelet volume was higher in the AH-CSU group (11.2 ± 0.3 fL) than in the CSU group (11.1 ± 0.4 fL; p = 0.002) and in the control group (10.3 ± 0.4 fL; p < 0.001). There were no significant differences in the mean levels of lymphocytes, monocytes, eosinophils, basophils, and platelets, and the rates of eosinopenia and basopenia between the patients in the AH-CSU group who responded to and those who were resistant to omalizumab. Conclusion: This study provided additional data of interest to examine the pathophysiologic role of low-grade inflammation and basopenia in patients with CSU and resistant to AHs and omalizumab.

Intermediate metabolism in association with the amino acid profile during the third trimester of normal pregnancy and diet-controlled gestational diabetes

2007 ◽  
Vol 196 (1) ◽  
pp. 65.e1-65.e5 ◽  
Author(s):  
Kalliopi I. Pappa ◽  
George Vlachos ◽  
Marianna Theodora ◽  
Maria Roubelaki ◽  
Konstantina Angelidou ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gestational Diabetes ◽  
Normal Pregnancy ◽  
Amino Acid Profile ◽  
Third Trimester ◽  
The Third ◽  
Intermediate Metabolism

scholarly journals The resting salivary antimicrobial proteins and cortisol concentration in wrestlers during 12-week training

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Teresa Trochimiak ◽  
Elżbieta Hübner-Woźniak ◽  
Paweł Tomaszewski
Keyword(s):  
Total Protein ◽  
Amylase Activity ◽  
Immunoglobulin A ◽  
Cortisol Concentration ◽  
System Response ◽  
Control Group ◽  
Physical Strain ◽  
Time Points ◽  
The Third ◽  
Preparatory Period

Summary Study aim: the aim of the present study was to investigate the effect of 12-week training on saliva immunoendocrine response in collegiate male and female wrestlers. Material and methods: the control group was composed of men and women of the same age, not engaged in any sports activity except for physical education classes at the university. The examined athletes participated in a 12-week training program, which consisted of two sub-phases (preparatory period and competitive period). Saliva samples were collected at three time points: at the beginning (the first point), after six weeks of the preparatory period (the second point, which was the start of the competitive period) and after six weeks of the competitive period (the third point). Immunoglobulin A and cortisol concentration, and α-amylase activity were measured in saliva by respective ELISA kits. Immunoglobulin A was expressed as relative to total protein concentration (sIgA/total protein). Results: at the third time point, the sIgA/total protein ratio was significantly lower in female compared to male athletes. α-Amylase activity was lower in all examined athletes at all three time points compared to respective control groups. Conclusions: hormonal and mucosal antimicrobial system response can provide helpful information of body adaptive processes to physical strain as well as indicators of magnitude of training-induced stress.

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