Assessment of Calprotectin in blood and tissue of Acne Vulgaris patients of different clinical severities

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Ghada Fathy Mohamed ◽  
Ahmed Abdel fattah Afify ◽  
Waleed Abdel hady Ahmed ◽  
Basant Alaa Eldin Elsayed Mohamed Elsayed Eid
Keyword(s):  
Calcium Binding ◽  
Acne Vulgaris ◽  
S100 Protein ◽  
Previous History ◽  
Statistical Correlation ◽  
Control Group ◽  
Inflammatory Lesions ◽  
Severe Acne ◽  
Calprotectin Level ◽  
The Difference

Abstract Background Acne vulgaris is a multifactorial inflammatory skin disease of the pilosebaceous unit with complex pathogenesis. Calprotectin is a heterodimer of two calcium-binding proteins S100A8 and S100A9, which are members of the S100 protein family, and which play a role in various inflammatory processes, it is mainly present in the cytoplasm of neutrophils and expressed on the membrane of monocytes. Objective to assess the level of Calprotectin in blood and in tissue of AV patients and compare it with healthy controls. Beside correlate it with the degrees of severity of acne in order to further understand the role of Calprotectin in the pathogenesis of AV. Subjects and Methods This case-control study included 28 subjects who have AV, divided into four groups according to Global Acne Grading System: the first group included 7 patients with mild acne, the second group included 7 patients with moderate acne, the third group included 7 patients with severe acne, and the fourth group included 7 patients with very severe AV. In addition to a control group which included 28 apparently healthy individuals of matched age, sex and with no previous history of acne or active acne. All the patients were recruited from the outpatient clinic of Al Haud Al Marsoud hospital from September 2019 till January 2020. After taking the approval of research ethics committee (FMASU M S 22/2019) all subjects gave an informed consent to participate in this work. Results Our study showed that the increase in AV severity was not affected by the difference in gender, type of acne, nor disease duration. However, there was a significant correlation between plasma calprotectin level and AV severity. In addition to, There was a significant statistical correlation between the increase in the age of patients and the decrease in the severity of acne, which indicates that AV is more severe in younger patients than older ones. The correlation between the Plasma Calprotectin level in patients group and Tissue Calprotectin level in inflammatory lesions (papules) was non-significant. However, the correlation between the Plasma Calprotectin level in patients’ group and Tissue Calprotectin level in non-inflammatory lesions (comedones) was highly significant. Conclusion Plasma Calprotectin level showed significant relation with AV severity and presence of scar. The correlation between plasma Calprotectin level and tissue Calprotectin level in noninflammatory lesions (comedones) was highly significant.

scholarly journals The Role of P53 in Sepsis-Induced Cardiomyopathy

2021 ◽  
Vol 26 (3) ◽  
pp. 45-47
Author(s):  
Peter Michael Reil ◽  
Teodor Traian Maghiar ◽  
Karlheinz Seidl ◽  
Claudia Teodora Judea Pusta ◽  
Ciprian Borza ◽  
...  
Keyword(s):  
Cardiac Tissue ◽  
Medical Problem ◽  
Statistical Correlation ◽  
Control Group ◽  
Study Group ◽  
Septic Cardiomyopathy ◽  
Pulmonary Infections ◽  
P53 Expression ◽  
The Difference

Abstract Septic cardiomyopathy remains a difficult medical problem to manage in critically ill patients. With all currently available therapeutic options, the mortality rate in these patients remains high. Our study included 29 patients diagnosed clinically with sepsis. A control group was used to compare the results. In all patients, p53 expression was assessed in cardiac tissue obtained from these patients and a statistical correlation was made with clinical data. The different expression rates of p53 do not correlate with patient’s age, having appropriate means in years, but with an increasing tendency with increasing expression (p=0.2110). The pulmonary infections are responsible for the majority of the septic state in the study group (over 55%). The difference between the infection sites is statistically significant (p<0.0001).

scholarly journals Is Oral Omega-3 Effective in Reducing Mucocutaneous Side Effects of Isotretinoin in Patients with Acne Vulgaris?

2018 ◽  
Vol 2018 ◽  
pp. 1-4 ◽  
Author(s):  
Mina Mirnezami ◽  
Hoda Rahimi
Keyword(s):  
Side Effects ◽  
Adverse Effects ◽  
Acne Vulgaris ◽  
Control Group ◽  
Case Group ◽  
Omega 3 ◽  
Double Blind ◽  
Severe Acne ◽  
Double Blind Clinical Trial ◽  
And Control

Background. Acne vulgaris is an inflammatory disease of pilosebaceous units which may cause permanent dyspigmentation and/or scars if not treated. Isotretinoin is recommended in the treatment of recalcitrant or severe acne, but it is associated with common adverse effects that frequently result in patients incompliance and discontinuation of the drug. The present study was designed to assess the efficacy of oral omega-3 in decreasing the adverse effects of isotretinoin. Materials and Methods. In this randomized double-blind clinical trial, a total of 118 patients with moderate or severe acne were randomly divided into two (case and control) groups. The control group was treated with isotretinoin 0.5 mg/kg, and the case group was treated with the same dose of isotretinoin combined with oral omega-3 (1 g/day). The treatment was lasted for 16 weeks and mucocutaneous side effects of isotretinoin were recorded and compared between the two groups in weeks 4, 8, 12, and 16. Results. Cheilitis (at weeks 4, 8, and 12), xerosis, dryness of nose at all weeks, and dryness of eyes (at week 4) were less frequent in the group that received isotretinoin combined with oral omega-3 compared to the group that received isotretinoin alone. Conclusion. Administration of oral omega-3 in acne patients who are receiving isotretinoin decreases the mucocutaneous side effects of isotretinoin. This trial is registered with  IRCT201306238241N2.

scholarly journals The Effectiveness of Pulsed Dye Laser in Management of Acne Vulgaris: A Systematic review and meta-analysis

2021 ◽  
pp. 41
Author(s):  
Keyword(s):  
Systematic Review ◽  
Laser Therapy ◽  
Treatment Option ◽  
Meta Analysis ◽  
Acne Vulgaris ◽  
Dye Laser ◽  
Pulsed Dye Laser ◽  
Control Group ◽  
The Mean ◽  
The Difference

Background: Acne vulgaris is one of the most prevalent skin diseases, affecting up to 85% of teenagers and young adults. Multiple treatment options including topical, systemic or physical therapies. However, the effects of conventional therapies are limited due to antibi-otic resistance and adverse effects such as irritation and teratogenicity of isotretinoin. Light-based therapy is an alternative and/or adjuvant therapy in patients who cannot tolerate or unresponsive to conventional therapies that may provide fewer side effects, patient com-fort, fast onset of action, and with equal or greater effectiveness. We aimed to assess the effectiveness of using Pulsed Dye Laser as a treatment option for acne vulgaris. A systemat-ic Review and Meta-analysis was peformed of randomized clinical trials assessing the ef-fectiveness of Pulsed Dye Laser in management of acne vulgaris. Methods: Medline Pubmed, Scopus, Cochrane library, the reference list, conference pro-ceedings, researchers in field of eligible studies were searched. Eight studies (n=275 sub-jects) were included in qualitative analysis of which six studies (n=190 subjects) were in-cluded in meta-analysis. The mean age of the participant was 21,94 years old. Intervention using application of Pulsed dye laser as monotherapy or combination in acne vulgaris pa-tients with follow-up at least 12 weeks. and the outcomes is a decrease mean of acne vul-garis lesions counts after received pulsed dye laser therapy. Results: Pooling of data using random effects model showed that the group that received PDL therapy alone or in combination had a lower number of acne lesions than the control group with the mean difference was -0.593 (95% CI = -1.290 to 0.104), the z value was -1.668 with p = 0.095. This shows that the number of lesions in the group that received sin-gle or combined PDL therapy was lower than the control group. However the difference is insignificant. Conclusion. From the results of the systematic review conducted, it can be concluded that in the group given Pulsed Dye Laser therapy there was a decrease in the total number of acne vulgaris lesions compared to before treatment, and the decrease, which was indicated by the difference in the mean number of acne lesions, was significantly greater compared to the control who was only given placebo. Pulsed Dye Laser therapy can be an alternative treatment option for acne vulgaris in patients who are unresponsive to previous treatment or conditions where there are contraindications to systemic therapy.

scholarly journals Assessment of Post SARS CoV 2 Fatigue among Physicians Working in COVID Designated Hospitals in Dhaka, Bangladesh

2021 ◽  
Author(s):  
A T M Hasibul Hasan ◽  
Muhammad Sougatul Islam ◽  
Nushrat Khan ◽  
Nazmul Hoque Munna ◽  
Wahidur Rahman Choton ◽  
...  
Keyword(s):  
Previous History ◽  
Disease Outbreaks ◽  
Control Group ◽  
Public And Private ◽  
Infectious Disease Outbreaks ◽  
Significant Difference ◽  
History Of ◽  
The Difference ◽  
Public And Private Hospitals ◽  
And Control

AbstractBackgroundFatigue has been observed after a number of infectious disease outbreaks around the world. After the outbreak of SARS CoV-2 in Wuhan, China in 2019, the disease turned into a pandemic very rapidly. Mental health is a key issue associated with such outbreaks. To explore the fatigue level among physicians working in designated public and private hospitals in Bangladesh, we conducted a matched case-control study of post-SARS-CoV-2 fatigue.MethodIn this study 105 physicians who were diagnosed as COVID-19 infected, got treatment, and declared cured at least 6 weeks before the interview date, were recruited as cases and the same number of age and designation matched healthy physicians as control who are working in the same hospital. Case and control were selected in 1:1 ratio from each of the hospitals. The study population was selected by inclusion and exclusion criteria after taking informed written consent. Data collection was done by a semi-structured questionnaire. Diagnosis of COVID--19 infection was done by detection of SARS CoV-2 antigen by RT-PCR from reference laboratories in Bangladesh or by HRCT Chest.ResultAround two-thirds of the physicians were male (67.6% versus 32.4%). Most of them aged less than forty years (80.5%). The cases had a greater number of comorbid conditions than those who were negative. The FSS score (mean) was much higher for cases (36.7 ± 5.3 versus 19.3 ± 3.8) than the control group with a statistically significant difference with no significant gender differentiation. Similarly, around 67.7% of the previously COVID positive physicians represented in the highest FSS score tertile compared to the respondents in the control group had a mean score of less than 3. The difference was also highly significant.ConclusionPhysicians, who had a previous history of COVID-19 infection had a higher total and mean FSS score, signifying a more severe level of fatigue than the physicians who had never been COVID-19 positive while working in the same hospital irrespective of their age and sex.

scholarly journals Efficacy of Oral Isotretinoin in Combination with Desloratadine in the Treatment of Common Vulgaris Acne in Vietnamese Patients

2019 ◽  
Vol 7 (2) ◽  
pp. 217-220
Author(s):  
Thuong Nguyen Van ◽  
Lan Duong Thi ◽  
Hao Nguyen Trong ◽  
Tro Chau Van ◽  
Trang Trinh Minh ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Side Effects ◽  
Treatment Time ◽  
Acne Vulgaris ◽  
Control Group ◽  
Inflammatory Lesions ◽  
Comparative Clinical Trial ◽  
Oral Isotretinoin ◽  
The Mean

AIM: To evaluate the efficacy of oral isotretinoin used alone and in combination with desloratadine in the treatment of moderate acne vulgaris. METHODS: A comparative clinical trial was undertaken to evaluate the efficacy of oral isotretinoin alone and in combination with desloratadine in the treatment of 62 moderate acne vulgaris patients. Patients were randomised into two groups with 31 patients in each group. Each studied group's patient took 20 mg isotretinoin and 5 mg desloratadine per day. In the control group, patients took only 20 mg isotretinoin per day. The treatment time was 16 weeks. The evaluation and follow-up were done at week 2, 4, 8, 12 and 16 of the treatment. RESULTS: The studied group had a better curative rate than the control group (45.2% versus 22.6%). The average number of inflammatory lesions in the studied group was significantly lower than the control group (0.19 versus 0.94). The mean GAGS score of the studied group was significantly lower than the control group (3.71 versus 6.52). Acne outbreaks rate of the studied group was lower than the control group (in week 2: 22.6% versus 45.2% and in week 4: 16.1% versus 38.7%, respectively). The rate of itchy was lower in the studied group. CONCLUSION: In the treatment of moderate acne vulgaris, oral isotretinoin in combination with desloratadine is more effective and has fewer side effects than using isotretinoin alone.

scholarly journals Clinical and Immunological Response to Doxycycline Versus Doxycycline Plus Vitamin C in Patients with Acne Vulgaris

2020 ◽  
Vol 11 (01) ◽  
pp. 09-11
Author(s):  
Zahraa I.J. Shubber ◽  
Entisar J. Al Mukhtar ◽  
Ifad K. Al-Shibly
Keyword(s):  
Vitamin C ◽  
Acne Vulgaris ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Significant Elevation ◽  
Once Daily ◽  
Severe Acne ◽  
Tnf Α ◽  
Ifn Γ

Objective: Study the influence of doxycycline versus doxycycline with vitamin C drugs in the management of acne vulgaris on some immunological parameters which include ( IL-1β, IL-8, IFN-γ, TNF-α, and TLR2) and the following objectives were adapted: 1-Selection of population for the study ( control, patients) groups and follow up the patients after one month of treatment, the first group receive doxycycline and the second group receive doxycycline with vitamin C,2- Blood samples collection and separation of serums for immunological analysis, 3- Statistical analysis. Methods: This study is a randomized clinical trial carried out in clinical dermatology in Merjan Medical City in Babylon from September 2018 to March 2019. The number of subjects enrolled in the present study was 60; their age was between (14- 30 years), among whom 30 were acne patients, the remaining 30 subjects were apparently healthy individuals, and they were served as control. A dermatologist diagnosed a total of 30 acne patients to having moderate to severe acne and divided into two groups (15 patients in each group). Patients in the first group were treated with doxycycline (100mg) once daily after meal for 30 days, while in the second group patients were treated with doxycycline (100mg) capsule once daily after a meal in combination with vitamin C (500mg) chewable tablets once daily. After 1-month of therapy, the response was evaluated clinically and immunologically by measure the concentration of pro-inflammatory cytokines (IL-8, IL-1β, IFN-γ, TNF-α) and ( TLR-2 ) by using enzyme-linked immunosorbent assay (ELISA) and the results were compared to their levels before treatment and that in the control group. Results: Significant elevation in the serum levels ( p ≤ 0.001) of immunological parameter IL-8, IL-1β, IFN-γ, TNF-α, and TLR-2 among acne patients in comparison to the control. The clinical response in the first group was good, moderate and poor in 5 (33%), 7(47%) and 3(20%) respectively, while in the second group was good, moderate and poor in 7 (47%), 7(47%) and 1(6%) respectively, the immunological results showed that the serum levels to the (IL-1β, IL-8, IFN-γ, TNF-α) and (TLR-2 ) were more reduced in the second group compared to their levels in the first group. Conclusion: Significant elevation (p less than 0.001) in the serum levels of (IL-8, IL-1β, IFN-γ, TNF-α, and TLR-2) among moderate to severe acne patients in comparison to control group. Clinically the combination of doxycycline plus vitamin C was more efficient as therapeutically in comparison to doxycycline alone. Immunologically doxycycline plus vitamin C was more effective in reducing serum levels of (IL-8, IL-1β, IFN-γ, TNF-α, and TLR-2) in comparison to doxycycline alone.

scholarly journals Evaluation of Bowel Habits in Patients with Acne Vulgaris

2021 ◽  
Author(s):  
Munise Daye ◽  
Fatma Cihan ◽  
Begüm Işık ◽  
Berna Hafızoğlu
Keyword(s):  
Patient Group ◽  
Short Form ◽  
Acne Vulgaris ◽  
Control Group ◽  
Case Group ◽  
Control Groups ◽  
Severe Acne ◽  
Bowel Habits ◽  
Significant Difference ◽  
And Control

Purpose: To evaluate the bowel habits of patients with acne vulgaris Method: In this case control study, socio-demographic characteristics of the participants (age, gender, marital status, educational status, profession, economic status, smoking-alcohol habits, chronic diseases, previous operations, people they live with, diet) were questioned and the global acne grading scores of the patients were calculated. Bristol Stool Scale, Rome III Criteria for IBS, International Physical Activity Questionnaire-Short Form, Hospital Anxiety Depression and KADF (dietary fiber information) scales fulfilled. Results: The patient group consisted 102 and the control group consisted 104 participants. The mean age was 20.9 ± 3.9 years old in the case group and 21.8 ± 5.0 years old in the control group. Patient and control groups were similar in terms of age and gender. Smoking rates were significantly higher in the patient group (p=0,035). The amount of coffee consumed per day were significantly higher in the patient group (p=0,040). According to the global acne grading scores, 55.9% (n = 57) had medium, 39.2% (n = 40) had mild, 3.9% (n = 4) had severe and 1% (n=1) had very severe acne. Anxiety scores were found to be significantly higher in the patient group (p = 0.005). When the case and control groups were compared for IBS presence, no significant difference was found (p = 0.317). Also, IBS was not related with acne severity (p = 0.162). Conclusion: Further large sample sized studies are needed on this subject, as there is strong evidence about brain–gut–skin axis existence.

Acne

2018 ◽  
Author(s):  
Ben Esdaile
Keyword(s):  
Hair Follicle ◽  
Sebaceous Gland ◽  
Acne Vulgaris ◽  
Psychological Effects ◽  
Inflammatory Disorder ◽  
Pilosebaceous Unit ◽  
Inflammatory Lesions ◽  
Severe Acne ◽  
Chronic Inflammatory Disorder

Acne vulgaris is a common, chronic inflammatory disorder of the pilosebaceous unit (the hair follicle and accompanying sebaceous gland). It can be classified as mild, moderate, or severe. Mild acne is characterized by comedones (non-inflammatory lesions: ‘blackheads’ and ‘whiteheads’) being the predominant lesions. Papules and pustules may also be present but are few in number. Moderate acne is defined by more inflammatory lesions such as papules and pustules, with comedones also usually present. Severe acne is defined as widespread inflammatory lesions, nodules, cysts, and scarring. Moderate acne that has not settled within 6 months of treatment, or acne that is causing serious psychological effects, is also categorized as severe.

scholarly journals Carotid intima medial thickness as a marker of atherosclerosis in ankylosing spondylitis

2018 ◽  
Vol 6 (6) ◽  
pp. 2068
Author(s):  
Sarvagya Mishra ◽  
Abhishek Singhai ◽  
Praksah Joshi ◽  
Rajesh Kumar Jha
Keyword(s):  
Ankylosing Spondylitis ◽  
Subclinical Atherosclerosis ◽  
Previous History ◽  
Vascular Complications ◽  
Clinical History ◽  
Axial Skeleton ◽  
Control Group ◽  
Inflammatory Disorder ◽  
Cross Sectional ◽  
The Difference

Background: Ankylosing Spondylitis (AS) is an inflammatory disorder of unknown cause that primarily affects the axial skeleton; peripheral joints and extraarticular structures are also frequently involved. The present study was planned to generate more data on this subject with the aim of measuring the CIMT in AS, as these patients are more prone to develop early atherosclerosis and develop early macro vascular complications like CAD.Methods: It was a cross-sectional study with a sample size of 60 patients. The study group included 30 patients with a known history or clinical features suggestive of AS. The control group included 30 normal adult subjects without any previous history. All subjects included in the study underwent detailed clinical history, analysis, physical examination and necessary investigations.Results: CIMT in the control group was 0.54±0.19 mm and in the AS group was 0.65±0.21 mm. The difference was found to be statistically significant (P<0.05), showing a higher CIMT in the AS group in comparison to the control group. The mean age in the control group was 30.43±6.14 years and in the AS group was 29.3±10.1 years. The difference was found to be statistically not significant (p>0.05), showing comparable age between the two groups. The age to CIMT showed positive correlation which was statistically significant (r=0.405, p=0.026).Conclusions: Results of this study showed that there is a higher prevalence of subclinical atherosclerosis (20%) in AS patients as compared to controls as evidenced by a higher CIMT. Every effort should be made in order to control inflammation and traditional risk factors in this population, to avoid the consequences of accelerated atherogenesis.

scholarly journals The Difference in Interleukin-19 Serum on Degrees of Acne Vulgaris Severity

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Moerbono Mochtar ◽  
Alamanda Murasmita ◽  
M. Eko Irawanto ◽  
Indah Julianto ◽  
Harijono Kariosentono ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Acne Vulgaris ◽  
Serum Levels ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Severe Acne ◽  
Significant Difference ◽  
The Difference ◽  
Degrees Of Severity

Introduction. Acne vulgaris is a multifactorial disease. Recent study showed that inflammation does have a central role in the formation of both inflammatory and noninflammatory lesions in acne vulgaris. There are various findings of proinflammatory cytokines related to acne vulgaris, but no previous study correlate interleukin- (IL-) 19 to acne vulgaris. This pilot study aims to look at difference in IL-19 serum concentration on degrees of severity of acne vulgaris. Methods. This is an analytical observational cross-sectional study. Sample subjects were patients with acne vulgaris who met the inclusion criteria. Enzyme-linked immunosorbent assay (ELISA) study was applied to measure IL-19 serum. Result. Analysis test found statistically significant difference between IL-19 serum concentration of group of patients with mild acne vulgaris and that of group of patients with severe acne vulgaris. Moreover, analysis revealed significant difference between IL-19 serum concentration of group of patients with moderate acne vulgaris and that of group of patients with severe acne vulgaris. Conclusions. There are differences in serum levels of IL-19 on the severity of acne vulgaris. The significant difference might show that inflammation has a core role in severity of acne vulgaris, and IL-19 might potentially be related to acne vulgaris.

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