Toxicity of Indian Mustard and Allyl Isothiocyanate to Masked Chafer Beetle Larvae

2002 ◽  
Vol 3 (1) ◽  
pp. 9 ◽  
Author(s):  
Ryan R. P. Noble ◽  
Stephanie G. Harvey ◽  
Carl E. Sams
Keyword(s):  
Brassica Juncea ◽  
Insecticidal Activity ◽  
Methyl Bromide ◽  
Larval Mortality ◽  
Indian Mustard ◽  
Allyl Isothiocyanate ◽  
Soil Mass ◽  
Soil Atmosphere ◽  
Phase Out

Alternatives for control of soil-inhabiting pests are needed due to the phase-out of methyl bromide. One possible alternative is using the pesticidal properties of compounds released by macerated Brassica tissues. In this study, larvae of masked chafer beetles (Cyclocephala spp.) were placed in soil amended with Brassica juncea L. (PI 458934) tissue. Allyl isothiocyanate (AITC) levels were positively correlated to larval mortality, with the 8% B. juncea treatment resulting in 100% larval mortality with an average AITC concentration of 11.4 mg per liter of soil atmosphere. Although B. juncea produces high levels of AITC, the mass of tissue required for significant insecticidal activity against Cyclocephala spp. also is high, between 4 and 8% of soil mass for this plant accession. Accepted for publication 23 May 2002. Published 10 June 2002.

scholarly journals 622 Toxicity of Indian Mustard (Brassica juncea) and Allyl Isothio-cyanate to Masked Chaffer Beetle Larvae (Cyclocephala sp.)

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 554F-555 ◽  
Author(s):  
Ryan R.P. Noble ◽  
C.S. Charron ◽  
C.E. Sams
Keyword(s):  
Brassica Juncea ◽  
Methyl Bromide ◽  
Solid Phase ◽  
Indian Mustard ◽  
Allyl Isothiocyanate ◽  
Soil Mass ◽  
Soil Bulk Density ◽  
Alternative Methods ◽  
Untreated Soil ◽  
Clean Air

The development of alternative methods for control of soilborne pathogens is imperative since the U.S. Clean Air Act bans the use of methyl bromide after 2005. One possibility is to exploit the pesticidal properties of compounds released by macerated Brassica tissues. In this study, masked chaffer beetle larvae were placed in sealed 473-mL jars with 335 g of soil amended with 1%, 2%, 4%, or 8% (g·g–1) Brassica tissue. The most prevalent volatile toxic compound of Brassica juncea (PI 458934) is allyl isothiocyanate (AITC). AITC production was measured in the jars at 0.25, 4, 8, 24, and 48 h using a solid-phase microextraction device (SPME) and gas chromatography. After 7 days, larvae mortality was determined. Control treatments included untreated soil, soil amended with 8% tomato plant tissue, soil amended with pure AITC, and untreated soil with an atmosphere of ≈20% O2 and 0% CO2 changing over 48 h to 2% O2 and 20% CO2. AITC levels were positively correlated to larvae mortality. The estimated lethal concentration for 50% kill (LC50) was 3.6 μg AITC/L soil atmosphere. AITC levels may be influenced by Brassica mass added, soil bulk density, and environmental factors including temperature and moisture. B. juncea has a high tissue AITC concentration. However, the mass of Brassica tissue required for insecticidal application against Cyclocephala sp. is also high, between 4% and 8% of soil mass. Development and selection of Brassica species that produce higher concentrations of isothiocyanate would increase the effectiveness of Brassica biofumigation as an alternative to methyl bromide for controlling soilborne insects.

scholarly journals Bio-Efficacy Of Some Plant Leaf Extracts Against Mustard Aphid, Lipaphis Erysimi Kalt. On Indian Mustard, Brassica Juncea

2010 ◽  
Vol 50 (2) ◽  
pp. 130-132 ◽  
Author(s):  
Arshad Ali ◽  
Parvez Rizvi ◽  
Farmanur Khan
Keyword(s):  
Brassica Juncea ◽  
Insecticidal Activity ◽  
Indian Mustard ◽  
Lipaphis Erysimi ◽  
Calotropis Procera ◽  
Leaf Extracts ◽  
Argemone Mexicana ◽  
Mustard Aphid ◽  
Plant Leaf ◽  
Tagetes Minuta

Bio-Efficacy Of Some Plant Leaf Extracts Against Mustard Aphid,Lipaphis ErysimiKalt. On Indian Mustard,Brassica JunceaThe bio-efficacy of four plant leaf extractsviz., apple of sodom,Calotropis proceraAiton; Mexican poppy,Argemone mexicanaLinnaeus; Mexican marigold,Tagetes minutaLinnaeus and Indian neem,Azadirachta indicawere tested against mustard aphid,Lipaphis erysimion Indian mustard,Brassica juncea. The highest per cent aphid reduction during first, second and third spray were 28.79, 40.52 and 59.32 at 1 : 10 g/ml; 34.70, 44.49 and 66.14 at 1 : 5 g/ml and 53.88, 64.84 and 100.00 at 1 : 2.5 g/ml with Indian neem. However, Mexican marigold was also effective at highest concentration (1 : 2.5 g/ml) and reduced 96.38 per centL. erysimi. All the treatments of plant leaf extracts showed insecticidal activity, but Indian neem followed by Mexican marigold reduced the aphid population to a great extent.

Interactive Effects of Sulfur and Nitrogen Supply on the Concentration of Sinigrin and Allyl Isothiocyanate in Indian Mustard (Brassica juncea L.)

2009 ◽  
Vol 57 (9) ◽  
pp. 3837-3844 ◽  
Author(s):  
Jóska GerendÁs ◽  
Jana PodestÁt ◽  
Thorsten Stahl ◽  
Kerstin Kübler ◽  
Hans Brückner ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Indian Mustard ◽  
Allyl Isothiocyanate ◽  
Nitrogen Supply ◽  
Interactive Effects

scholarly journals Allyl Isothiocyanate and Carbon Dioxide Produced during Degradation of Brassica juncea Tissue in Different Soil Conditions

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1734-1739 ◽  
Author(s):  
Andrew J. Price ◽  
Craig S. Charron ◽  
Arnold M. Saxton ◽  
Carl E. Sams
Keyword(s):  
Brassica Juncea ◽  
Sandy Loam ◽  
Indian Mustard ◽  
Sampling Technique ◽  
Allyl Isothiocyanate ◽  
Soil Conditions ◽  
Clay Loam ◽  
Clay Loam Soil ◽  
Main Effect ◽  
Loam Soil

A study was conducted to quantify volatiles generated from Indian mustard (Brassica juncea L. Czerniak) tissue incorporated into soils under controlled conditions. Mustard residues were incorporated into noncovered and covered soils that varied by texture, temperature, moisture, pH, or sterility (autoclaved or nonautoclaved). Sandy loam soil had 38% more allyl isothiocyanate (AITC) than clay loam soil. AITC concentration in 45 °C soil was 81% higher than in soil at 15 °C, and 56% higher in covered compared to noncovered treatments. The microbial catabolism of AITC was suggested by the result that AITC concentration in autoclaved soils was over three times that measured in nonautoclaved soils. The highest AITC level detected (1.71 μmol·L–1) occurred in the autoclaved covered soil. Several factors also influenced CO2 evolution. At 30 or 45 °C, CO2 concentration was at least 64% higher than at 15°C. The covered soil had over twice the CO2 found in the noncovered soil, and the nonautoclaved soil treatment yielded twice the CO2 measured in the autoclaved soil. There were no main effect differences among soil moisture, soil pH, and soil texture treatments for CO2 concentrations. This information could be helpful in defining ideal soil conditions for field scale experiments. Additionally, this study demonstrates a sampling technique for testing fumigation potential of biofumigation and solarization systems that may have the potential to replace methyl bromide.

Agronomic performance of autumn- and winter-cropped Indian mustard (Brassica juncea L.) in response to varying levels of nitrogen fertiliser

2020 ◽  
pp. 83-95
Author(s):  
Bent Al-Hoda Asghari ◽  
Mohsen Yousefi ◽  
Katarzyna Możdżeń ◽  
Joanna Puła ◽  
Peiman Zandi ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Seed Yield ◽  
Agronomic Traits ◽  
Growth Parameters ◽  
Indian Mustard ◽  
Climatic Conditions ◽  
Plant Seed ◽  
Nitrogen Fertiliser ◽  
Nitrogen Levels ◽  
Cropping Seasons

Indian mustard (Brassica juncea L. Czern) cultivation is suggested for regions with short seasons and low rainfall. Although there have been many studies conducted on agronomic production of mustard in Iran, the information regarding the interactive impact of cropping seasons and nitrogen fertiliser on growth characteristics and yield quality of mustard plant is still insufficient and requires further investigation. This study focused on the possible implications of different cropping seasons and different nitrogen levels on selected agronomic traits in mustard. In this experiment, five different doses of nitrogen and two sowing periods were used to assess for their combined effects on the growth parameters, seed yield and agronomic characteristics of mustard in the semi-arid climatic conditions of Takestan. The results revealed that cultivation seasons and nitrogen rates had a significant effect on plant height, biomass yield, number of siliques per plant, seed oil content and seed yield.

Metam Sodium Combined with Chloropicrin as an Alternative to Methyl Bromide Fumigation for Tomato

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 525b-525 ◽  
Author(s):  
S.J. Locascio ◽  
D.W. Dickson
Keyword(s):  
United States ◽  
Lycopersicon Esculentum ◽  
Pest Control ◽  
Methyl Bromide ◽  
Soil Fungi ◽  
Good Control ◽  
The United States ◽  
Metam Sodium ◽  
Additional Water ◽  
Phase Out

In past work, dichloropropene + 17% Pic (1,3-D + Pic) at 327 L·ha–1 plus pebulate provided good control of nematode, soil fungi, and nutsedge in mulched tomato (Lycopersicon esculentum Mill.) and is considered the best alternative for methyl bromide (MBr) + chloropicrin (Pic), which is scheduled for phase-out in the United States by Jan. 2001. Metam-sodium did not provide acceptable pest control. In the present study, metam-Na (295 L·ha–1 combined with Pic (168 kg·ha–1) + 4.5 kg·ha–1 pebulate, and 1,3-D + 35% Pic at 168 and 225 L·ha–1 + pebulate were compared to MBr-Pic (98-2% at 345 kg·ha–1 and 67-33% at 505 kg·ha–1). Fumigants were injected into the bed except metam-Na and pebulate were surface-applied and incorporated and drip tubing and mulch were applied. Marketable yields with MBr-Pic, 225 L·ha–1 1,3-D + Pic, and metam-Na + Pic were higher than with the check. Yields with metam-Na alone or with additional water before transplanting were similar to the check. Nutsedge was controlled with MBr-Pic and all treatments with pebulate. Nematode root-gall ratings were high on tomato grown without fumigants (8.9 rating on a scale of 0 to 10 with 0 = no galling), low with MBr-Pic (0.33), and intermediate with all other treatments (2.2 to 5.5) except with 168 L·ha–1 1-3-D + Pic (8.3). This study indicates that metam-Na + Pic + pebulate also is a possible alternative to MBr-Pic for tomato.

Inhibition of Fungal Growth by Macerated Plant Tissues in a Closed Environment

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 476e-476
Author(s):  
Craig S. Charron ◽  
Catherine O. Chardonnet ◽  
Carl E. Sams
Keyword(s):  
Radial Growth ◽  
Fungal Pathogens ◽  
Solid Phase ◽  
Indian Mustard ◽  
Fungal Growth ◽  
Allyl Isothiocyanate ◽  
Pythium Ultimum ◽  
Alternative Methods ◽  
Gas Chromatography Mass Spectrometry ◽  
Clean Air

The U.S. Clean Air Act bans the use of methyl bromide after 2001. Consequently, the development of alternative methods for control of soilborne pathogens is imperative. One alternative is to exploit the pesticidal properties of macerated tissues of Brassica spp. This study tested the potential of several Brassica spp. for control of fungal pathogens. Pythium ultimum Trow or Rhizoctonia solani Kühn plugs on potato-dextrose agar on petri dishes were sealed in 500-ml glass jars (at 22 °C) containing macerated leaves (10 g) from one of six Brassica spp. Radial growth was measured 24, 48, and 72 h after inoculation. Indian mustard (B. juncea) was the most suppressive, followed by `Florida Broadleaf' mustard (B. juncea). Volatile compounds in the jars were sampled with a solid-phase microextraction device (SPME) and identified by gas chromatography-mass spectrometry (GC-MS). Allyl isothiocyanate (AITC) comprised over 90% of the total volatiles measured from Indian mustard and `Florida Broadleaf' mustard. Isothiocyanates were detected in jars with all plants except broccoli. (Z)-3-hexenyl acetate was emitted by all plants and was the predominant volatile of `Premium Crop' broccoli (B. oleracea L. var. italica), `Michihili Jade Pagoda' Chinese cabbage (B. pekinensis), `Charmant' cabbage (B. oleracea L. var. capitata), and `Blue Scotch Curled' kale (B. oleracea L. var. viridis). To assess the influence of AITC on radial growth of P. ultimum and R. solani, AITC was added to jars to give headspace concentrations of 0.10, 0.20, and 0.30 mg·L–1 (mass of AITC per volume of headspace). Growth of both fungi was inhibited by 0.10 mg·L–1 AITC. 0.20 mg·L–1 AITC was fungicidal to P. ultimum although the highest AITC level tested (0.30 mg·L–1) did not terminate R. solani growth. These results indicate that residues from some Brassica spp. may be a viable part of a soilborne pest control strategy.

Chemical Constituents in Some Promising Genotypes of Indian Mustard [Brassica juncea (L.) Czern and Coss.]

2017 ◽  
Vol 4 (04) ◽  
Author(s):  
SUNITA SINGH ◽  
R. P. SINGH ◽  
H. K. SINGH ◽  
N. A. KHAN ◽  
M. K. MAURYA
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Brassica Juncea ◽  
Acid Composition ◽  
Chemical Constituents ◽  
Indian Mustard ◽  
Acid Value ◽  
Fatty Acid Analysis ◽  
Crude Fibre

Among the oilseed Brassica crops, Indian mustard [Brassica juncea (L.) Czern and Coss.] is an important source of oil from a nutritional point of view. The nutritional value of oil and cake quality is governed mainly by the composition of its fatty acids, iodine value, saponification, acid value, glucosinolates, crude fibre, protein and limiting amino acids, etc. Seventeen varieties/strains of Indian mustard were taken for saturated and unsaturated fatty acid analysis. The eicosenoic was absent in genotype (NUDBYJ-10) and erucic acid (NUDBYJ-10, LES-46 and Pusa mustard- 21). The fatty acid composition found a variable in different genotypes. Saturated fatty acid, Palmitic + Stearic ranged between 2.3 to 6.5%, Oleic 10.6 to 40.7%, Linoleic 16.1 to 37.7%, Linolenic 13.3 to 26.7%, Eicosenoic 0.00 to 10.30% and Erucic acid 0.00 to 47.50%, respectively. Alternaria blight severity also varied in different genotypes and ranged between 18.75 to 56.25%, maximum being in genotype Kranti and minimum in LES-47. No significant correlation was observed between the fatty acid composition and disease severity. The oil content range from 38.1 to 42.60% and protein content was found highest in variety RGN-73. The amino acid viz. methionine and tryptophan range between 0.41 to 1.81 g/16gN and 0.41 to 1.81 g /16g N, respectively.

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