Effect of Ascorbic Acid, Silicon and Iron on Collagen Synthesis in the Human Dermal Fibroblast Cell(HS27)

This first-attempt study tended to inspect the cytotoxic effects of montmorillonite (MMT) or 0.01 N phosphoric acid treated MMT supplemented with L-ascorbic acid (LAA) upon human dermal fibroblasts for possible applications. Light micrographs of human dermal fibroblast cell cultures revealed that more dense black spots in larger sizes were observed when higher levels of MMT were supplemented into the fibroblast culture, indicating that more dermal fibroblasts were covered by MMT particles. Compared with the supplementation of LAA alone, this study selected mitochondrial dehydrogenase activity (MTT) assay as an indicator bioreaction to show possible cytotoxic (or allergic) responses upon human dermal fibroblasts in vitro when LAA/acid-treated MMT composites were added. Statistical analysis showed that LAA augmented with either MMT or 0.01 N phosphoric-acid-treated MMT provoked insignificant cytotoxic responses to human dermal fibroblasts. Thus, an augmentation of MMT or 0.01 N phosphoric-acid-treated MMT to LAA should be biologically feasible for possible skin applications according to this human dermal fibroblasts model.

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An Anti-Inflammatory 2,4-Cyclized-3,4-Secospongian Diterpenoid and Furanoterpene-Related Metabolites of a Marine Sponge Spongia sp. from the Red Sea

Marine Drugs ◽

10.3390/md19010038 ◽

2021 ◽

Vol 19 (1) ◽

pp. 38

Author(s):

Chi-Jen Tai ◽

Chiung-Yao Huang ◽

Atallah F. Ahmed ◽

Raha S. Orfali ◽

Walied M. Alarif ◽

...

Keyword(s):

Red Sea ◽

Superoxide Anion ◽

Dermal Fibroblast ◽

Fibroblast Cell ◽

Inhibitory Effect ◽

Human Dermal Fibroblast ◽

Superoxide Anion Generation ◽

Potent Inhibitory Effect ◽

Anti Inflammatory ◽

New Compounds

Chemical investigation of a Red Sea Spongia sp. led to the isolation of four new compounds, i.e., 17-dehydroxysponalactone (1), a carboxylic acid, spongiafuranic acid A (2), one hydroxamic acid, spongiafuranohydroxamic acid A (3), and a furanyl trinorsesterpenoid 16-epi-irciformonin G (4), along with three known metabolites (−)-sponalisolide B (5), 18-nor- 3,17-dihydroxy-spongia-3,13(16),14-trien-2-one (6), and cholesta-7-ene-3β,5α-diol-6-one (7). The biosynthetic pathway for the molecular skeleton of 1 and related compounds was postulated for the first time. Anti-inflammatory activity of these metabolites to inhibit superoxide anion generation and elastase release in N-formyl-methionyl-leucyl phenylalanine/cytochalasin B (fMLF/CB)-induced human neutrophil cells and cytotoxicity of these compounds toward three cancer cell lines and one human dermal fibroblast cell line were assayed. Compound 1 was found to significantly reduce the superoxide anion generation and elastase release at a concentration of 10 μM, and compound 5 was also found to display strong inhibitory activity against superoxide anion generation at the same concentration. Due to the noncytotoxic activity and the potent inhibitory effect toward the superoxide anion generation and elastase release, 1 and 5 can be considered to be promising anti-inflammatory agents.

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Wound Healing Potential of Spirulina Protein on CCD-986sk Cells

Marine Drugs ◽

10.3390/md17020130 ◽

2019 ◽

Vol 17 (2) ◽

pp. 130

Author(s):

Ping Liu ◽

Jeong-Wook Choi ◽

Min-Kyeong Lee ◽

Youn-Hee Choi ◽

Taek-Jeong Nam

Keyword(s):

Wound Healing ◽

Dermal Fibroblast ◽

Fibroblast Cell ◽

Underlying Mechanism ◽

Human Dermal Fibroblast ◽

Dermal Fibroblasts ◽

Cyclin Dependent Kinase ◽

Pi3k Inhibitor Ly294002 ◽

And Migration ◽

Proliferation And Migration

Wound healing is a dynamic and complex process. The proliferation and migration of dermal fibroblasts are crucial for wound healing. Recent studies have indicated that the extracts from Spirulina platensis have a positive potential for wound healing. However, its underlying mechanism is not fully understood. Our previous study showed that spirulina crude protein (SPCP) promoted the viability of human dermal fibroblast cell line (CCD-986sk cells). In this study, we further investigated the wound healing effect and corresponding mechanisms of SPCP on CCD-986sk cells. Bromodeoxyuridine (BrdU) assay showed that SPCP promoted the proliferation of CCD-986sk cells. The wound healing assay showed that SPCP promoted the migration of CCD-986sk cells. Furthermore, cell cycle analysis demonstrated that SPCP promoted CCD-986sk cells to enter S and G2/M phases from G0/G1 phase. Western blot results showed that SPCP significantly upregulated the expression of cyclin D1, cyclin E, cyclin-dependent kinase 2 (Cdk2), cyclin-dependent kinase 4 (Cdk4), and cyclin-dependent kinase 6 (Cdk6), as well as inhibited the expression of CDK inhibitors p21 and p27 in CCD-986sk cells. In the meanwhile, SPCP promoted the phosphorylation and activation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). However, the phosphorylation of Akt was significantly blocked by PI3K inhibitor (LY294002), which in turn reduced the SPCP-induced proliferation and migration of CCD-986sk cells. Therefore, the results presenting in this study suggested that SPCP can promote the proliferation and migration of CCD-986sk cells; the PI3K/Akt signaling pathway play a positive and important role in these processes.

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Modulation of heat shock protein 90 affects TGF-β-induced collagen synthesis in human dermal fibroblast cells

Tissue and Cell ◽

10.1016/j.tice.2016.09.002 ◽

2016 ◽

Vol 48 (6) ◽

pp. 616-623 ◽

Cited By ~ 7

Author(s):

Sae Bin Lee ◽

A-ram Lim ◽

Dong Kyun Rah ◽

Kyung Soo Kim ◽

Hyun Jin Min

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Collagen Synthesis ◽

Dermal Fibroblast ◽

Heat Shock Protein 90 ◽

Human Dermal Fibroblast ◽

Fibroblast Cells

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Effects ofPanax ginsengextract on human dermal fibroblast proliferation and collagen synthesis

International Wound Journal ◽

10.1111/iwj.12530 ◽

2015 ◽

Vol 13 ◽

pp. 42-46 ◽

Cited By ~ 11

Author(s):

Geum-Young Lee ◽

Kang-Gyun Park ◽

Sik Namgoong ◽

Seung-Kyu Han ◽

Seong-Ho Jeong ◽

...

Keyword(s):

Collagen Synthesis ◽

Dermal Fibroblast ◽

Human Dermal Fibroblast ◽

Fibroblast Proliferation

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Fabrication of Chitosan Nanofibers Scaffolds with Small Amount Gelatin for Enhanced Cell Viability

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.749.220 ◽

2015 ◽

Vol 749 ◽

pp. 220-224 ◽

Cited By ~ 1

Author(s):

Min Sup Kim ◽

Sang Jun Park ◽

Bon Kang Gu ◽

Chun Ho Kim

Keyword(s):

Mechanical Properties ◽

Cell Culture ◽

Cell Viability ◽

Dermal Fibroblast ◽

Fibroblast Cell ◽

Biological Properties ◽

Human Dermal Fibroblast ◽

Initial Contact ◽

Initial Contact Angle ◽

Contact Angle Analysis

Chitosan and gelatin has attracted considerable interest owing to its advantageous biological properties such as excellent biocompatibility, biodegradation, and non-toxic properties. In this paper, we investigated the potential of chitosan/gelatin (Chi-Gel) nanofibers mat with enhanced cell viability for use as cell culture scaffolds. The surface morphology, mechanical properties, and initial contact angle analysis of Chi-Gel nanofibers mat were evaluated. The proliferation of human dermal fibroblast cell (HDFs) on Chi-Gel nanofibers mat was found to be approximately 20% higher than the pure chitosan nanofibers mat after 7 days of culture. These results suggest that the Chi-Gel nanofibers mat has great potential for use tissue engineering applications.

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Dermal Olfactory Receptor OR51B5 Is Essential for Survival and Collagen Synthesis in Human Dermal Fibroblast (Hs68 Cells)

International Journal of Molecular Sciences ◽

10.3390/ijms22179273 ◽

2021 ◽

Vol 22 (17) ◽

pp. 9273

Author(s):

Bomin Son ◽

Wesuk Kang ◽

Soyoon Park ◽

Dabin Choi ◽

Taesun Park

Keyword(s):

Cell Survival ◽

Collagen Synthesis ◽

Dermal Fibroblast ◽

Olfactory Receptors ◽

Camp Response Element Binding ◽

Human Dermal Fibroblast ◽

Skin Aging ◽

Dermal Fibroblasts ◽

Nasal Tissue ◽

Extracellular Matrix Components

Skin dermis comprises extracellular matrix components, mainly collagen fibers. A decrease in collagen synthesis caused by several factors, including ultraviolet (UV) irradiation and stress, eventually causes extrinsic skin aging. Olfactory receptors (ORs) were initially considered to be specifically expressed in nasal tissue, but several ORs have been reported to be present in other tissues, and their biological roles have recently received increasing attention. In this study, we aimed to characterize the role of ORs in cell survival and collagen synthesis in dermal fibroblasts. We confirmed that UVB irradiation and dexamethasone exposure significantly decreased cell survival and collagen synthesis in Hs68 dermal fibroblasts. Moreover, we demonstrated that the mRNA expression of 10 ORs detectable in Hs68 cells was significantly downregulated in aged conditions compared with that in normal conditions. Thereafter, by individual knockdown of the 10 candidate ORs, we identified that only OR51B5 knockdown leads to a reduction of cell survival and collagen synthesis. OR51B5 knockdown decreased cAMP levels and dampened the downstream protein kinase A/cAMP-response element binding protein pathway, downregulating the survival- and collagen synthesis-related genes in the dermal fibroblasts. Therefore, OR51B5 may be an interesting candidate that plays a role in cell survival and collagen synthesis.

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