CYTOTOXIC ASSESSMENT OF L-ASCORBIC ACID/MONTMORILLONITE UPON HUMAN DERMAL FIBROBLASTS IN VITRO: MTT ACTIVITY ASSAY

2008 ◽  
Vol 20 (06) ◽  
pp. 337-343
Author(s):  
Yuan-Haun Lee ◽  
Bor-Yann Chen ◽  
Feng-Huei Lin ◽  
Kun-Yu Lin ◽  
King-Fu Lin
Keyword(s):  
Ascorbic Acid ◽  
Phosphoric Acid ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Cytotoxic Effects ◽  
Black Spots

This first-attempt study tended to inspect the cytotoxic effects of montmorillonite (MMT) or 0.01 N phosphoric acid treated MMT supplemented with L-ascorbic acid (LAA) upon human dermal fibroblasts for possible applications. Light micrographs of human dermal fibroblast cell cultures revealed that more dense black spots in larger sizes were observed when higher levels of MMT were supplemented into the fibroblast culture, indicating that more dermal fibroblasts were covered by MMT particles. Compared with the supplementation of LAA alone, this study selected mitochondrial dehydrogenase activity (MTT) assay as an indicator bioreaction to show possible cytotoxic (or allergic) responses upon human dermal fibroblasts in vitro when LAA/acid-treated MMT composites were added. Statistical analysis showed that LAA augmented with either MMT or 0.01 N phosphoric-acid-treated MMT provoked insignificant cytotoxic responses to human dermal fibroblasts. Thus, an augmentation of MMT or 0.01 N phosphoric-acid-treated MMT to LAA should be biologically feasible for possible skin applications according to this human dermal fibroblasts model.

scholarly journals Wound Healing Potential of Spirulina Protein on CCD-986sk Cells

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 130
Author(s):  
Ping Liu ◽  
Jeong-Wook Choi ◽  
Min-Kyeong Lee ◽  
Youn-Hee Choi ◽  
Taek-Jeong Nam
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Underlying Mechanism ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Cyclin Dependent Kinase ◽  
Pi3k Inhibitor Ly294002 ◽  
And Migration ◽  
Proliferation And Migration

Wound healing is a dynamic and complex process. The proliferation and migration of dermal fibroblasts are crucial for wound healing. Recent studies have indicated that the extracts from Spirulina platensis have a positive potential for wound healing. However, its underlying mechanism is not fully understood. Our previous study showed that spirulina crude protein (SPCP) promoted the viability of human dermal fibroblast cell line (CCD-986sk cells). In this study, we further investigated the wound healing effect and corresponding mechanisms of SPCP on CCD-986sk cells. Bromodeoxyuridine (BrdU) assay showed that SPCP promoted the proliferation of CCD-986sk cells. The wound healing assay showed that SPCP promoted the migration of CCD-986sk cells. Furthermore, cell cycle analysis demonstrated that SPCP promoted CCD-986sk cells to enter S and G2/M phases from G0/G1 phase. Western blot results showed that SPCP significantly upregulated the expression of cyclin D1, cyclin E, cyclin-dependent kinase 2 (Cdk2), cyclin-dependent kinase 4 (Cdk4), and cyclin-dependent kinase 6 (Cdk6), as well as inhibited the expression of CDK inhibitors p21 and p27 in CCD-986sk cells. In the meanwhile, SPCP promoted the phosphorylation and activation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). However, the phosphorylation of Akt was significantly blocked by PI3K inhibitor (LY294002), which in turn reduced the SPCP-induced proliferation and migration of CCD-986sk cells. Therefore, the results presenting in this study suggested that SPCP can promote the proliferation and migration of CCD-986sk cells; the PI3K/Akt signaling pathway play a positive and important role in these processes.

scholarly journals Resveratrol-Loaded Levan Nanoparticles Produced by Electrohydrodynamic Atomization Technique

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2582
Author(s):  
Ezgi Cinan ◽  
Sumeyye Cesur ◽  
Merve Erginer Haskoylu ◽  
Oguzhan Gunduz ◽  
Ebru Toksoy Oner
Keyword(s):  
Dermal Fibroblast ◽  
Halophilic Bacteria ◽  
Particle Diameter ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Delivery Systems ◽  
Cellular Interaction ◽  
In Vitro Biocompatibility ◽  
Electrohydrodynamic Atomization

Considering the significant advances in nanostructured systems in various biomedical applications and the escalating need for levan-based nanoparticles as delivery systems, this study aimed to fabricate levan nanoparticles by the electrohydrodynamic atomization (EHDA) technique. The hydrolyzed derivative of levan polysaccharide from Halomonas smyrnensis halophilic bacteria, hydrolyzed Halomonas levan (hHL), was used. Nanoparticles were obtained by optimizing the EHDA parameters and then they were characterized in terms of morphology, molecular interactions, drug release and cell culture studies. The optimized hHL and resveratrol (RS)-loaded hHL nanoparticles were monodisperse and had smooth surfaces. The particle diameter size of hHL nanoparticles was 82.06 ± 15.33 nm. Additionally, release of RS from the fabricated hHL nanoparticles at different pH conditions were found to follow the first-order release model and hHL with higher RS loading showed a more gradual release. In vitro biocompatibility assay with human dermal fibroblast cell lines was performed and cell behavior on coated surfaces was observed. Nanoparticles were found to be safe for healthy cells. Consequently, the fabricated hHL-based nanoparticle system may have potential use in drug delivery systems for wound healing and tissue engineering applications and surfaces could be coated with these electrosprayed particles to improve cellular interaction.

Ultrastructural and metabolic effects of products of activated mononuclear leucocytes on cultured human dermal fibroblasts

1984 ◽  
Vol 42 ◽  
pp. 682-683
Author(s):  
I. Stachura ◽  
Matias Pardo ◽  
Jennifer Worrall ◽  
Theresa L. Whiteside
Keyword(s):  
Plasma Membranes ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Metabolic Effects ◽  
Normal Human Skin ◽  
Normal Human ◽  
Mononuclear Leucocytes ◽  
Distinctive Phenotype

Products of antigen- or mitogen-activated mononuclear leucocytes (ML) are known to modulate fibroblast proliferation and collagen production in vitro. In tissue, ML accumulate at sites of inflammation and are probably involved in the process of fibrosis. We have established that supernatants (SN) of concanavalin A-activated ML increase synthesis of glycosaminoglycan (GAG) in human dermal fibroblast (DF) cultures). When explants of normal human skin were cultured in CMRL 1066 medium supplemented with 15% pooled human serum and containing MLSN the outgrowing DF acquired a distinctive phenotype. In comparison to control DF, the cells treated with MLSN exhibited a marked increase in the number of intracytoplasmic organelles especially dilated cisternae of RER filled with electrondense material, abundant lysosomes, prominent Golgi apparatus and bundles of microfilaments often extending beyond the cell boundaries. Cell surfaces were shaggy and floccular material accumulated in patches along the plasma membranes.

scholarly journals In Vitro Cytotoxic Protective Effect of Alginate-Encapsulated Capsaicin Might Improve Skin Side Effects Associated with the Topical Application of Capsaicin

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1455
Author(s):  
Ariana Hudita ◽  
Bianca Galateanu ◽  
Marieta Costache ◽  
Carolina Negrei ◽  
Rodica-Mariana Ion ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Side Effects ◽  
Cell Viability ◽  
Dermal Fibroblast ◽  
Expression Profiles ◽  
Control Culture ◽  
Dermal Fibroblasts ◽  
Diabetic Patients ◽  
Cytotoxic Effects

Chronic neuropathic pain, particularly peripheral pain, is a cause of great concern for diabetic patients. Current treatments include numerous agents such as capsaicinoids, a known deterrent of neuropathic pain despite the inconvenience associated with local side effects. In this context, the current work aims to elucidate the potential mechanisms involved in cytotoxicity by capsaicin and proposes an efficient formulation of capsaicin in alginate microcapsules, which significantly reduces side effects from capsaicin topical administration. For this, human dermal fibroblast cells were treated with alginate-microencapsulated capsaicin extracts and screened for potential cytotoxic effects produced by the treatment. Cell viability and morphology were examined, as well as oxidative stress status and anti-inflammatory potential. Our results show that the alginate encapsulated formulation of capsaicin exerted lower cytotoxic effects on human dermal fibroblasts as measured by cell viability and reactive oxygen species (ROS) production. Furthermore, the expression profiles of inflammatory cytokines were significantly altered by the treatment as compared with the control culture.

Effects Of (1→3), (1→6)-β-D-Glucan Behavior in Human Dermal Fibroblast Cells under Serum Starvation

2007 ◽  
Vol 342-343 ◽  
pp. 401-404 ◽  
Author(s):  
Yeon I Woo ◽  
Hyun Joo Son ◽  
Hye Ryeon Lim ◽  
Mi Hee Lee ◽  
Hyun Sook Baek ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Positive Influence ◽  
Dermal Fibroblasts ◽  
Serum Starvation ◽  
Healing Agent ◽  
And Migration ◽  
Free Serum

Glucans have been reported to stimulate immunity and to promote wound healing. Adult human dermal fibroblast (aHDF) cultured in serum free (serum-starvation). Proliferation of aHDF was measured at various concentrations of β-glucan by MTT assay, and migration was observed for 36h on microscope. The result of fibroblast bioassay, β-glucan had positive influence. In this study, the direct effects of β-glucan on proliferation and migration of human dermal fibroblasts were examined in vitro. That means β-D-glucan has the effect to enhance proliferation and aHDF migration speed, and has the potential as a wound healing agent.

scholarly journals Bactericidal/Permeability-Increasing Protein Is Expressed by Human Dermal Fibroblasts and Upregulated by Interleukin 4

2003 ◽  
Vol 10 (3) ◽  
pp. 473-475 ◽  
Author(s):  
Philipp H. Reichel ◽  
Christine Seemann ◽  
Elena Csernok ◽  
Jens-M. Schröder ◽  
Antje Müller ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Dermal Fibroblast ◽  
Constitutive Expression ◽  
Human Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Interleukin 4 ◽  
Human Dermal Fibroblasts ◽  
Bacterial Clearance ◽  
Gram Negative

ABSTRACT The bactericidal/permeability-increasing protein (BPI) is an antibiotic- and endotoxin-neutralizing protein of granulocytes and epithelial cells. Constitutive expression of BPI, which increases upon interleukin 4 stimulation, by human dermal fibroblast was demonstrated, suggesting an important role of BPI in gram-negative bacterial clearance and a dampened response to endotoxin in the skin.

scholarly journals Pengaruh Glukosa Tinggi terhadap Proliferasi, Migrasi dan Ekspresi Gen OCT-4 pada Kultur Sel Dermal Fibroblast Manusia

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Restu Syamsul Hadi ◽  
Yurika Sandra
Keyword(s):  
Dermal Fibroblast ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Minimal Essential Medium ◽  
Rt Pcr ◽  
Scratch Assay

Human dermal fibroblasts (HDF) termasuk sel mesenchymal yang diisolasi dari lapisan dermis kulit. HDF berpotensi digunakan untuk pengobatan penyembuhan luka berdasarkan pengobatan regeneratif. Peningkatan konsentrasi glukosa dapat merusak fungsi sel dan menghambat terapi penyembuhan luka. Penelitian ini dilakukan untuk mengkaji pengaruh glukosa tinggi pada proliferasi, migrasi dan ekspresi gen OCT-4 sel HDF sebagai model penyembuhan luka diabetes in vitro. Pada penelitian eksperimental ini, fibroblast diisolasi dari kulit setelah sirkumsisi, kemudian ditumbuhkan dalam medium Minimal Essential Medium (DMEM) Dulbecco lengkap dengan serum 10%. Untuk menguji efek glukosa tinggi pada proliferasi sel HDF dilakukan dengan uji CCK-8. Migrasi sel HDF dievaluasi menggunakan uji scratch-assay. RT-PCR digunakan untuk menentukan ekspresi gen OCT-4.  Hasilnya menunjukkan bahwa glukosa tinggi (25 mM-50 mM) meningkatkan kemampuan proliferasi dan migrasi sel HDF. Efek glukosa tinggi tergantung pada dosis. Perlakuan glukosa pada dosis 75 mM akan menghambat kemampuan pertumbuhan HDF. Ekspresi gen OCT-4 meningkat secara bermakna pada pemberian glukosa dosis 25-50 mM. Hasil penelitian ini memberikan dasar untuk pengembangan terapi dalam kondisi diabetes bahwa terapi sel HDF dapat meningkatkan penyembuhan luka meskipun dalam kondisi glukosa tinggi.

scholarly journals Physico-Chemical Characterization and In Vitro Biological Evaluation of a Bionic Hydrogel Based on Hyaluronic Acid and l-Lysine for Medical Applications

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1194
Author(s):  
Giuseppe Alonci ◽  
Roberto Mocchi ◽  
Sabrina Sommatis ◽  
Maria Chiara Capillo ◽  
Elsa Liga ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Dermal Fibroblast ◽  
Chemical Characterization ◽  
Fibroblast Cell ◽  
Human Dermal Fibroblast ◽  
Biological Evaluation ◽  
Medical Applications ◽  
Biological Compatibility ◽  
3D Network

Hyaluronic acid (HA) is an endogenous polysaccharide, whose hydrogels have been used in medical applications for decades. Here, we present a technology platform for stabilizing HA with a biocrosslinker, the amino acid L-lysine, to manufacture bionic hydrogels for regenerative medicine. We synthetized bionic hydrogels with tailored composition with respect to HA concentration and degree of stabilization depending on the envisaged medical use. The structure of the hydrogels was assessed by microscopy and rheology, and the resorption behavior through enzymatic degradation with hyaluronidase. The biological compatibility was evaluated in vitro with human dermal fibroblast cell lines. HA bionic hydrogels stabilized with lysine show a 3D network structure, with a rheological profile that mimics biological matrixes, as a harmless biodegradable substrate for cell proliferation and regeneration and a promising candidate for wound healing and other medical applications.

Sign in / Sign up

Export Citation Format

Share Document