Effect of Propofol on Affective State as Assessed by Place Conditioning Paradigm in Rats

1996 ◽  
Vol 85 (1) ◽  
pp. 121-128 ◽  
Author(s):  
L. Pain ◽  
P. Oberling ◽  
G. Sandner ◽  
G. Di Scala
Keyword(s):  
Free Choice ◽  
Recovery Period ◽  
Affective State ◽  
Conditioning Session ◽  
Place Preference ◽  
The Other ◽  
Choice Test ◽  
Place Conditioning ◽  
Short Term ◽  
Conditioning Paradigm

Background Whether propofol produces a pleasant affective state remains unclear from clinical studies. In the current study, the effect on affective state of subanesthetic and anesthetic doses of propofol was assessed at a preclinical level with rats in a place conditioning paradigm. Propofol was compared with methohexital. Methods In the place conditioning paradigm, propofol-induced effect was repeatedly paired with one of two distinguishable compartments of the apparatus, whereas the vehicle-induced effect was repeatedly paired with the other compartment. During a subsequent free-choice test, a preference for the drug-paired compartment over the vehicle-paired compartment would be indicative of pleasant state induced by the drug. For all experiments, the conditioning session lasted 8 days and consisted of four pairings of the drug with one compartment and four pairings of the equivalent volume of vehicle with the other compartment. In experiment 1A, four groups of rats were designated according to the dose of propofol that they received intraperitoneally: 0,30,60, or 90 mg/kg. In experiment 1B, the same procedure was used with subanesthetic doses of intraperitoneal methohexital: 0,10,20, or 30 mg/kg. In experiment 2, the rats were conditioned during the recovery period from short-term anesthesia. For one group, anesthesia was induced by propofol (100 mg/kg) whereas for the other group, anesthesia was induced by an equivalent anesthetic dose of methohexital (40 mg/kg). Results In experiment 1A, the 30-mg/kg, 60-mg/kg, and 90-mg/kg groups showed a place preference for the drug-paired compartment, but only the group conditioned with 60 mg/kg propofol significantly differed from the 0-mg/kg group. In experiment 1B, the groups conditioned with methohexital showed no place preference for the drug-paired compartment. In experiment 2, the rats showed a place preference for the compartment in which they recovered from propofol-induced anesthesia but no place preference for the compartment in which they recovered from methohexital-induced anesthesia. Conclusions Propofol, but not methohexital, induced a pleasant affective state in rats at subanesthetic doses as well as during recovery from an anesthetic dose.

Effect of Midazolam on Propofol-induced Positive Affective State Assessed by Place Conditioning in Rats 

1997 ◽  
Vol 87 (4) ◽  
pp. 935-943 ◽  
Author(s):  
Laure Pain ◽  
Philippe Oberling ◽  
Guy Sandner ◽  
Georges Di Scala
Keyword(s):  
Affective State ◽  
Place Preference ◽  
The Other ◽  
Choice Test ◽  
Place Conditioning ◽  
Affective States ◽  
Positive Affective State ◽  
Subsequent Choice ◽  
Experimental Cage ◽  
Recovery From Anesthesia

Background The effect of either midazolam or the combination of midazolam and propofol on the affective state was assessed in rats at subanesthetic doses and at recovery from anesthesia. Methods The putative drug(s)-induced affective states were repeatedly paired with one of two distinguishable compartments of an experimental cage, whereas the vehicle(s)-induced effect was repeatedly paired with the other compartment. During a subsequent choice test for one compartment over the other, the rats' preference for the drug(s)-paired compartment over the vehicle(s)-paired compartment is indicative of a pleasant state induced by the drug(s). In experiment 1, rats were conditioned with different doses of midazolam either at subanesthetic states or at recovery from anesthesia. In experiment 2, groups of rats were conditioned with different combinations of midazolam and propofol either at subanesthetic states or at recovery from anesthesia induced jointly by midazolam (10 mg/kg) and propofol (60 mg/kg). Experiment 3 was conducted in the same way as experiment 2, except that midazolam was paired with both compartments. In addition, these groups were tested not only in an undrugged state but also in a drugged (with midazolam) state. Results In experiment 1, rats exhibited a place preference for the environment previously associated with midazolam, at subanesthetic and anesthetic doses. Experiment 2 showed that a propofol-induced place preference was found to be dose-dependently suppressed by midazolam. Experiment 3 replicated the findings of experiment 2 and extended them to the mechanism by which midazolam blocked a propofol-induced place preference. Conclusions Midazolam administered before propofol blocked the expression of a propofol-induced pleasant state.

scholarly journals Study of Host Preference and the Comparison of some Biological Characteristcs of Bemisia Tabasi (Genn) on Tomato Varieties

2013 ◽  
Vol 53 (2) ◽  
pp. 137-142
Author(s):  
Masoomeh Samareh Fekri ◽  
Mohammad Amin Samih ◽  
Sohrab Imani ◽  
Mehdi Zarabi
Keyword(s):  
Free Choice ◽  
Host Preference ◽  
Resistance Mechanism ◽  
Total Mortality ◽  
Statistical Design ◽  
The Other ◽  
Choice Test ◽  
Percentage Mortality ◽  
Tomato Variety ◽  
Choice Preference

Abstract The resistance of 8 tomato varieties to cotton white fly, Bemisia tabaci (Genn) (Hemiptera: Aleyrodidae), was evaluated in four greenhouse experiments. In the first experiment, we evaluated the attractiveness and preference for oviposition in a free-choice test (randomized blocks, 8 treatments, and 5 replications). In the other experiments, we evaluated the no-choice preference for oviposition (randomized blocks, 4 treatments, and 4 replications). The whitefly egg-adult cycle was monitored using a statistical design in randomized blocks with four replications. The percentage mortality of immature stages was also determined (randomized blocks with four replications). In the free-choice test, the tomato variety Rio Grande was the most attractive to adults, while the variety Cheffalat had the lowest number of adults. Also in this assay, the variety CAL-JN3 presented the lowest number of eggs, while the variety Ergon presented the highest number of eggs. In the no-choice test, the varieties Chef-falat and CAL-JN3 remained resistant. Consequently, for these two varieties non-preference is the oviposition resistance mechanism. The egg-adult cycle varied from 26.02 days (Ergon) to 26.66 days (CAL-JN3). The total mortality varied from 20.52 (Ergon) to 33.97 (CAL-JN3). Considering all the characteristics, the variety CAL-JN3 was the most resistant to B. tabaci among all the tomato varieties studied, while variety Ergon was susceptible.

scholarly journals High Tolerance of Trichogramma Pretiosum (Hymenoptera: Trichogrammatidae) to the Mixture of the Herbicides Nicosulfuron Plus Atrazine

2020 ◽  
Author(s):  
Germano Leão Demolin Leite ◽  
João Marcos Batista Cordeiro ◽  
Leonardo David Tuffi Santos ◽  
Wagner de Souza Tavares ◽  
Alexandre Igor de Azevedo Pereira ◽  
...  
Keyword(s):  
Sex Ratio ◽  
Free Choice ◽  
Integrated Control ◽  
The Other ◽  
Choice Test ◽  
Trichogramma Pretiosum ◽  
Maize Crop ◽  
Trichogramma Brasiliensis ◽  
Herbicide Mixture ◽  
Lepidopteran Pest

Abstract Background: Maize, Zea mays (Poaceae), is one of the most important crops in the world, but defoliating caterpillars (Lepidoptera) and weeds can reduce the productivity of this plant. The control of weeds in the maize crop in Brazil is mainly accomplished with nicosulfuron- and atrazine-based herbicides, and of lepidopteran pest eggs with use of several species of Trichogrammatidae (Hymenoptera) parasitoids released. The objective of this study was to evaluate the rates of parasitism and emergence of females and the sex ratio (%) of 10 Trichogrammatidae species, in a free-choice test, submitted to a mixture of the herbicides Sanson 40 SC® (a.i. nicosulfuron; dose: 30 g.ha–1) + Gesaprim 500 Ciba-Geigy® (a.i. atrazine; dose: 1500 g.ha–1) in the laboratory.Results: The parasitism rate (%) of Trichogramma acacioi Brun, Moraes & Soares, Trichogrammatoidea annulata de Santis, Trichogramma atopovirilia Oatman & Platner, Trichogramma bennetti Nagaraja & Nagarkatti, Trichogramma brunni Nagaraja, Trichogramma brasiliensis Ashmead, Trichogramma demoraesi Nagaraja, Trichogramma galloi Zucchi, and Trichogramma soaresi Nagaraja was lower with the herbicide mixture of nicosulfuron + atrazine than the control and, therefore, this mixture was considered to be slightly harmful (i.e., class II of the International Organization for Biological and Integrated Control) to these parasitoids. This solution reduced the emergence rate (%) of female T. acacioi, T. annulata, T. atopovirilia, T. bruni, T. brasiliensis, T. demoraesi, T. galloi, and T. soaresi, being classified as harmless to T. soaresi, moderately harmful to T. galloi and slightly harmful to the other species. The herbicide mixture of nicosulfuron + atrazine reduced the sex ratio of T. acacioi, T. atopovirilia, T. bruni, T. demoraesi, T. galloi, and T. soaresi, being classified as harmless to T. annulata and T. brasiliensis, and slightly harmful to the other species.Conclusions: The biological parameters (i.e., rates of parasitism and emergence and the sex ratio) of T. pretiosum, followed by T. bennetti, T. annulata and T. brasiliensis were the least affected by the herbicide mixture. These species should be chosen for release, on pieces of paperboard with parasitized E. kuehniella eggs, in crops together with the application of nicosulfuron + atrazine.

scholarly journals Neurons in the Locus Coeruleus Modulate the Hedonic Effects of Sub-Anesthetic Dose of Propofol

2021 ◽  
Vol 15 ◽  
Author(s):  
Hui Chen ◽  
Dan Xu ◽  
Yu Zhang ◽  
Yan Yan ◽  
JunXiao Liu ◽  
...  
Keyword(s):  
Locus Coeruleus ◽  
Neuronal Activity ◽  
Dopaminergic Neurons ◽  
Place Preference ◽  
Designer Drugs ◽  
Place Conditioning ◽  
General Anesthetic ◽  
Response Scale ◽  
Conditioning Paradigm

Propofol is a worldwide-used intravenous general anesthetic with ideal effects, but hedonic effects of propofol have been reported and cause addictive issue. There is little known about the neurobiological mechanism of hedonic effects of propofol. Increasing researches have shown that the dopaminergic nervous system of the ventral tegmental area (VTA) and the noradrenergic system of locus coeruleus (LC) play a crucial role in hedonic experiences, which are putative sites for mediating the hedonic effects of propofol. In the present study, rat hedonic response scale and place conditioning paradigm were employed to examine the euphoric effects of propofol. In vivo GCaMP-based (AVV-hSyn-GCaMP6s) fiber photometry calcium imaging was used to monitor the real-time neuronal activity in VTA and LC area in rats exhibiting propofol-induced euphoric behaviors. Then DREADDs (designer receptors exclusively activated by designer drugs) modulation using rAAV-hSyn-hM4D(Gi)-EGFP was performed to confirm the neuronal substrate that mediates the euphoric effects of propofol. The score of hedonic facial responses was significantly increased in the 4 mg/kg group compared with that of the 0 mg/kg group. The locomotor activity in the propofol-paired compartment was significantly increased at the 4 mg/kg dose compared with that of the saline-paired group. When compared with the 0 mg/kg group, the place preference increased in the 4 mg/kg group. Administration of 4 mg/kg of propofol triggers reliable increases in GcaMP fluorescence. However, in the VTA GcaMP-expressing rats, administration of 4 mg/kg of propofol did not induce any change of GcaMP signals. The facial score and the place preference, which increased by 4 mg/kg propofol were abolished by chemogenetic inhibition of the neuronal activity in the LC area. Our results suggest that LC noradrenergic neurons, not VTA dopaminergic neurons, are directly involved in the hedonic effects of sub-anesthetic dose of propofol.

scholarly journals Toxicological and behavioral impacts of atrazine on Trichogrammatidae (Hymenoptera) in choice tests

2021 ◽  
Vol 47 (1) ◽  
Author(s):  
Germano Leão Demolin Leite ◽  
Edilson Paulo Rodrigues Bispo ◽  
Anarelly Costa Alvarenga ◽  
Paula Daiana De Paulo ◽  
Marcus Alvarenga Soares ◽  
...  
Keyword(s):  
Natural Enemies ◽  
Free Choice ◽  
Test Tube ◽  
The Other ◽  
Choice Test ◽  
Choice Tests ◽  
Herbicide Atrazine ◽  
Lepidoptera Pyralidae ◽  
Glass Test Tube ◽  
And Control

Weeds should be controlled with low impact methods and selective agrochemicals that have little or no effect on non-target organisms. This study aimed to evaluate the effect of the herbicide atrazine (triazine class) on 10 Trichogrammatidae (Hymenoptera) species. A female of 10 species of these natural enemies was individually placed in a glass test tube (free-choice test) with two paper cards containing 45 Anagasta (Ephestia) kuehniella (Lepidoptera: Pyralidae) eggs (treatment and control), with 10 replications. The cards were sprayed with the herbicide atrazine at 8.07 L/ha; the control was sprayed with distilled water. Parasitism by these natural enemies was allowed for 48 h. Atrazine changes the behavior of female parasitoids, reducing the parasitism (≈ 71 %) and emergence (≈ 74 %) rates and sex ratio (≈ 74 %) of the Trichogrammatidae species, except for Trichogramma galloi and T. bennetti. No females emerged from eggs parasitized by T. acacioi, Trichogrammatoidea annulata, T. atopovirilia, T. bruni, T. brasiliensis, T. demoraesi, and T. soaresi with atrazine. The results revealed that atrazine herbicide is harmless to T. bennetti and T. galloi, but it was moderately harmful (80 – 99 % reduction) to the other Trichogrammatidae species based on the parasitism and emergence rates.

A Bird in the Hand Isn’t Good for Long

2018 ◽  
Vol 65 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Stefan Scherbaum ◽  
Simon Frisch ◽  
Maja Dshemuchadse
Keyword(s):  
Decision Making ◽  
Cognitive Control ◽  
The Other ◽  
Decision Task ◽  
Short Term ◽  
Additional Time ◽  
Control Processes ◽  
Folk Wisdom ◽  
Study Participants ◽  
Insight Into

Abstract. Folk wisdom tells us that additional time to make a decision helps us to refrain from the first impulse to take the bird in the hand. However, the question why the time to decide plays an important role is still unanswered. Here we distinguish two explanations, one based on a bias in value accumulation that has to be overcome with time, the other based on cognitive control processes that need time to set in. In an intertemporal decision task, we use mouse tracking to study participants’ responses to options’ values and delays which were presented sequentially. We find that the information about options’ delays does indeed lead to an immediate bias that is controlled afterwards, matching the prediction of control processes needed to counter initial impulses. Hence, by using a dynamic measure, we provide insight into the processes underlying short-term oriented choices in intertemporal decision making.

Hidden “holes” in the capital of banks and the supply of credit to the real sector of economy

2018 ◽  
pp. 49-68 ◽  
Author(s):  
M. E. Mamonov
Keyword(s):  
Credit Risk ◽  
The Other ◽  
Real Sector ◽  
Capital Management ◽  
Loan Loss ◽  
Short Term ◽  
The Real ◽  
Ex Post ◽  
The One

Our analysis documents that the existence of hidden “holes” in the capital of not yet failed banks - while creating intertemporal pressure on the actual level of capital - leads to changing of maturity of loans supplied rather than to contracting of their volume. Long-term loans decrease, whereas short-term loans rise - and, what is most remarkably, by approximately the same amounts. Standardly, the higher the maturity of loans the higher the credit risk and, thus, the more loan loss reserves (LLP) banks are forced to create, increasing the pressure on capital. Banks that already hide “holes” in the capital, but have not yet faced with license withdrawal, must possess strong incentives to shorten the maturity of supplied loans. On the one hand, it raises the turnovers of LLP and facilitates the flexibility of capital management; on the other hand, it allows increasing the speed of shifting of attracted deposits to loans to related parties in domestic or foreign jurisdictions. This enlarges the potential size of ex post revealed “hole” in the capital and, therefore, allows us to assume that not every loan might be viewed as a good for the economy: excessive short-term and insufficient long-term loans can produce the source for future losses.

Pengembangan Kemitraan Koperasi, Usaha Mikro Dan Kecil (KUMK) Dengan Usaha Menengah/Besar Untuk Komoditi Unggulan Lokal

2019 ◽  
Vol 9 (1) ◽  
pp. 53-66 ◽  
Author(s):  
Dandan Irawan
Keyword(s):  
Business Performance ◽  
The Other ◽  
Economic Conditions ◽  
Small Enterprises ◽  
Business Partnerships ◽  
Short Term ◽  
Business Partnership ◽  
Structure Factors ◽  
Micro And Small Enterprises ◽  
Business Entities

Basically a natural partnership will achieve its goal if mutual requirements, mutual reinforcement, and mutual benefit can be maintained and made a strong fundamental commitment among partners. Nevertheless the development seems very slow. The cause is the presence of specific and different conditions and structure factors compared to other countries. Along with that, we still encounter various forms of gaps, such as inequality among regions, among income groups, between sectors, among economic actors, and so forth. The next problem is that in business entities including cooperatives and micro and small enterprises in running their business activities requires business partnerships with medium and large enterprises in order to improve business performance and business scale. While on the other hand our economic conditions and structures are not yet fully conducive to fostering partnerships based on purely business considerations or competitive market motivations but the business partnership of the foundation is strong enough in our country's constitution. Partnerships will work if partners are equally benefiting. Our concept of partnership is like that, although in the short term, there is a party or a party benefiting more from the other side.

EFFECT OF ACTINOMYCIN D ON TSH-INDUCED STIMULATION OF THYROIDAL PROTEIN SYNTHESIS IN THE RAT

1974 ◽  
Vol 77 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Gustav Wägar
Keyword(s):  
Protein Synthesis ◽  
Actinomycin D ◽  
The Other ◽  
Leucine Incorporation ◽  
Short Term ◽  
Other Hand ◽  
Thyroid Glands ◽  
Translational Level ◽  
Stimulation Of

ABSTRACT Whether the short-term regulation of thyroidal protein synthesis by TSH occurs at the transcriptional or the translational level was tested by measuring the effect of actinomycin D (act D) on the TSH-induced stimulation of L-14C-leucine incorporation into the thyroidal proteins of rats. TSH was injected 6 h before the rats were killed. The thyroid glands were then removed and incubated in vitro in the presence of L-14C-leucine for 2 h. The pronounced stimulation of leucine incorporation in the TSH-treated animals was depressed as compared with controls but still significant even when the animals had been pre-treated with 100 μg act D 24 and 7 h before sacrifice. On the other hand, act D strongly decreased incorporation of 3H-uridine into RNA. Short-term regulation of thyroidal protein synthesis by TSH appears to be partly but not wholly dependent on neosynthesis of RNA. Hence regulation may partly occur at the translation level of protein synthesis.

scholarly journals Prediction of Head Movement in 360-Degree Videos Using Attention Model

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3678
Author(s):  
Dongwon Lee ◽  
Minji Choi ◽  
Joohyun Lee
Keyword(s):  
Machine Learning ◽  
Short Term Memory ◽  
Moving Average ◽  
The Other ◽  
Learning Models ◽  
Short Term ◽  
Term Memory ◽  
Attention Model ◽  
Long Short Term Memory ◽  
Machine Learning Models

In this paper, we propose a prediction algorithm, the combination of Long Short-Term Memory (LSTM) and attention model, based on machine learning models to predict the vision coordinates when watching 360-degree videos in a Virtual Reality (VR) or Augmented Reality (AR) system. Predicting the vision coordinates while video streaming is important when the network condition is degraded. However, the traditional prediction models such as Moving Average (MA) and Autoregression Moving Average (ARMA) are linear so they cannot consider the nonlinear relationship. Therefore, machine learning models based on deep learning are recently used for nonlinear predictions. We use the Long Short-Term Memory (LSTM) and Gated Recurrent Unit (GRU) neural network methods, originated in Recurrent Neural Networks (RNN), and predict the head position in the 360-degree videos. Therefore, we adopt the attention model to LSTM to make more accurate results. We also compare the performance of the proposed model with the other machine learning models such as Multi-Layer Perceptron (MLP) and RNN using the root mean squared error (RMSE) of predicted and real coordinates. We demonstrate that our model can predict the vision coordinates more accurately than the other models in various videos.

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