Determination and transdetermination in imaginal disks of
Drosophila
(Summary only)
It is generally assumed that in multicellular organisms the diversity of the different cell types is the result of different gene activity which becomes manifest in the course of development. This theoretical concept of cell differentiation was developed on the basis of results obtained from a relatively small number of suitable experimental systems. One of them comprises the imaginal disks of the fruitfly Drosophila melanogaster . Imaginal disks are larval primordia in holometabolic insects such as flies and mosquitoes, and consist of densely packed populations of morphologically uniform cells. They give rise to defined structures of the adult body (mainly integument), thus replacing parts of the larva which are almost completely histolysed during metamorphosis. The prospective fate of the various imaginal disks can be tested, for example, by transplantation experiments. Individual disks are removed from larvae of a genetically marked strain and transplanted into the body cavity of another larva with which the transplants undergo metamorphosis. The metamorphosed derivatives of the disks are then found in the abdomen of the fly and can be microscopically identified on the basis of the morphology of bristles, hairs and other structural features of the integument. The same method is applied for examination of the developmental performance of disk fragments. From the results of such experiments the following conclusions are drawn: (1) Individual disks of fully grown larvae, that is larvae which are ready to pupate, are determined (programmed) for exactly defined body parts of the adult organism. (2) The individual subregions of such a body part can be localized precisely within a disk. Based on these facts fate maps (anlage plans) can be worked out. (3) From experiments in which different genetically marked disks are intermingled and then transplanted into larvae it is concluded that even single cells are determined for structures of a specific body region.