scholarly journals Fibroblast migration and collagen deposition during dermal wound healing: mathematical modelling and clinical implications

2006 ◽  
Vol 364 (1843) ◽  
pp. 1385-1405 ◽  
Author(s):  
Steven McDougall ◽  
John Dallon ◽  
Jonathan Sherratt ◽  
Philip Maini
Keyword(s):  
Wound Healing ◽  
Diffusion Coefficient ◽  
Scar Tissue ◽  
Tissue Formation ◽  
Collagen Deposition ◽  
Dermal Wound Healing ◽  
Fibroblast Migration ◽  
Collagen Alignment ◽  
Dermal Wound ◽  
Scar Tissue Formation

The extent to which collagen alignment occurs during dermal wound healing determines the severity of scar tissue formation. We have modelled this using a multiscale approach, in which extracellular materials, for example collagen and fibrin, are modelled as continua, while fibroblasts are considered as discrete units. Within this model framework, we have explored the effects that different parameters have on the alignment process, and we have used the model to investigate how manipulation of transforming growth factor-β levels can reduce scar tissue formation. We briefly review this body of work, then extend the modelling framework to investigate the role played by leucocyte signalling in wound repair. To this end, fibroblast migration and collagen deposition within both the wound region and healthy peripheral tissue are considered. Trajectories of individual fibroblasts are determined as they migrate towards the wound region under the combined influence of collagen/fibrin alignment and gradients in a paracrine chemoattractant produced by leucocytes. The effects of a number of different physiological and cellular parameters upon the collagen alignment and repair integrity are assessed. These parameters include fibroblast concentration, cellular speed, fibroblast sensitivity to chemoattractant concentration and chemoattractant diffusion coefficient. Our results show that chemoattractant gradients lead to increased collagen alignment at the interface between the wound and the healthy tissue. Results show that there is a trade-off between wound integrity and the degree of scarring. The former is found to be optimized under conditions of a large chemoattractant diffusion coefficient, while the latter can be minimized when repair takes place in the presence of a competitive inhibitor to chemoattractants.

Transforming Growth Factors-?1 and ?2 Enhance Connective Tissue Formation in Animal Models of Dermal Wound Healing by Secondary Intent

1990 ◽  
Vol 593 (1 Transforming) ◽  
pp. 135-147 ◽  
Author(s):  
GEORGE A. KSANDER ◽  
GEORGE H. CHU ◽  
HUGH McMULLIN ◽  
YASUSHI OGAWA ◽  
BRUCE M. PRATT ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Animal Models ◽  
Connective Tissue ◽  
Tissue Formation ◽  
Transforming Growth Factors ◽  
Dermal Wound Healing ◽  
Dermal Wound

scholarly journals Hypoxia Preconditioned Serum (HPS)-Hydrogel Can Accelerate Dermal Wound Healing in Mice—An In Vivo Pilot Study

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 176
Author(s):  
Jun Jiang ◽  
Ursula Kraneburg ◽  
Ulf Dornseifer ◽  
Arndt F. Schilling ◽  
Ektoras Hadjipanayi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Lymphatic Vessels ◽  
Scar Tissue ◽  
Tissue Samples ◽  
Medicine Research ◽  
Dermal Wound Healing ◽  
Wound Model ◽  
Dermal Wound

The ability to use the body’s resources to promote wound repair is increasingly becoming an interesting area of regenerative medicine research. Here, we tested the effect of topical application of blood-derived hypoxia preconditioned serum (HPS) on wound healing in a murine wound model. Alginate hydrogels loaded with two different HPS concentrations (10 and 40%) were applied topically on full-thickness wounds created on the back of immunocompromised mice. We achieved a significant dose-dependent wound area reduction after 5 days in HPS-treated groups compared with no treatment (NT). On average, both HPS-10% and HPS-40% -treated wounds healed 1.4 days faster than NT. Healed tissue samples were investigated on post-operative day 15 (POD 15) by immunohistology and showed an increase in lymphatic vessels (LYVE-1) up to 45% with HPS-40% application, while at this stage, vascularization (CD31) was comparable in the HPS-treated and NT groups. Furthermore, the expression of proliferation marker Ki67 was greater on POD 15 in the NT-group compared to HPS-treated groups, in accordance with the earlier completion of wound healing observed in the latter. Collagen deposition was similar in all groups, indicating lack of scar tissue hypertrophy as a result of HPS-hydrogel treatment. These findings show that topical HPS application is safe and can accelerate dermal wound healing in mice.

scholarly journals A review on Vranagranthi (Keloid) in Ayurvedic and Modern Aspect

2019 ◽  
Vol 7 (06) ◽  
Author(s):  
Abhijeet Suresh Shinde
Keyword(s):  
Wound Healing ◽  
Scar Tissue ◽  
Review Article ◽  
Tissue Formation ◽  
Pathological Study ◽  
Modern Aspect ◽  
Surgical Scar ◽  
Severe Acne ◽  
Scar Tissue Formation ◽  
Regressive Phase

Hypertrophic appearing scar tissue formation is known as Keloids. The growth of keloid is as compare to other raised scars is more rapid and progressive. If the keloid is formed then it grow much larger than the wound that caused the scar. Keloid is hypertrophic appearing scar that continue to evolve over the time without quiescent or regressive phase in the process of wound healing [1,2]    The certain communities e.g. Africans having Keloid-prone skin. The cut, burn, pin prick or severe acne are the some examples that causes Keloid. A keloid can also form as chickenpox clear. Sometimes, a surgical scar becomes a keloid. In very rare cases, keloids form when people do not injure their skin. These are called “spontaneous keloids.” A keloid usually takes time to appear. After an injury, months can pass before this scar appears. A keloid can also form more quickly. Once it begins, a keloid can enlarge slowly for months or years. Ayurveda Vagbhatacharya described it is as Vrunagranthi. This review article is a comparative pathological study of Vrunagranthi with special references to keloid is found that having direct and much more identical Causes, features, pathology and prognostic between these.

The Temporal Course of Adenovirus-Mediated Gene Transfer in the Rat Larynx

2002 ◽  
Vol 126 (3) ◽  
pp. 281-290
Author(s):  
Jacobs Ian N. ◽  
Tufano Ralph P. ◽  
Walsh Danielle S. ◽  
Crombleholme Timothy
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Gene Transfer ◽  
Recombinant Adenovirus ◽  
Marker Gene ◽  
Care Facility ◽  
Scar Tissue ◽  
Complete Disappearance ◽  
Tissue Formation ◽  
Scar Tissue Formation

OBJECTIVE: Polypeptide growth factors have important influences on wound-healing and scar tissue formation. Specific growth factors or their inhibitors may potentially decrease scar tissue formation and prevent subglottic stenosis. Gene transfer using recombinant adenovirus may be an ideal method to mediate endogenous production of growth factors to inhibit fibrosis. STUDY DESIGN: The study incorporated adenovirus-mediated transduction of normal and stenotic rat larynges and histologic analysis of the sequential expression of a β-galactosidase marker gene over time. SETTING: The study was conducted at the animal care facility of an academic children's hospital. RESULTS: We report successful transduction in normal and injured rat larynx with peak expression of β-galactosidase at 2 days after transduction and almost complete disappearance by 7 days. There appeared to be an early inflammatory response to the viral injection, but at 7 and 14 days after injection (transduction) the uninjured rat larynges resumed a normal histologic appearance. All distant sites stained negative for β-galactosidase. CONCLUSION: Recombinant adenovirus-mediated gene transfer is feasible in the rat larynx with transient duration and limited toxicity. SIGNIFICANCE: Adenovirus-mediated gene transfer has the potential to deliver growth factors that modulate wound healing and inflammation in the larynx by inhibiting fibrosis.

scholarly journals Wound Healing Efficacy of Guava Leaf Extract

2021 ◽  
pp. 27-35
Author(s):  
Sarah B. Delorino ◽  
Madeline L. Ogalesco ◽  
Kaye R. Rebadulla ◽  
Myrelle Tricia A. Rongcales ◽  
Joshua Ismael A. Salubre V ◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Povidone Iodine ◽  
Biological Process ◽  
Scar Tissue ◽  
Tissue Formation ◽  
Complex Process ◽  
Healing Wound ◽  
Guava Leaf ◽  
Scar Tissue Formation

A wound is a break in the continuity of the skin. The body’s response to injury and the restoration of the same is healing. Wound healing is a biological process that is initiated by trauma and often terminated by scar formation. In this research, guava leaf extract was used as antiseptic and its efficacy was tested to commercially available products. The result revealed that all mice which received guava leaf extract formed scar earlier as compared to povidone iodine and PNSS. Since maturation phase which also refers to remodeling phase is responsible for the new epithelium and final scar tissue formation, and as the development of these completes the complex process of wound healing, the researchers suggest that the use of guava leaf extract in comparison with povidone iodine and PNSS when it comes to wound healing is the most affordable in treatment and promoting normal and more rapid wound healing.

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