scholarly journals Variation in maize chlorophyll biosynthesis alters plant architecture

2020 ◽  
Author(s):  
Rajdeep S. Khangura ◽  
Gurmukh S. Johal ◽  
Brian P. Dilkes
Keyword(s):  
Plant Height ◽  
Sweet Corn ◽  
Chlorophyll Biosynthesis ◽  
Growth Control ◽  
Magnesium Chelatase ◽  
Chlorophyll Contents ◽  
Dominant Mutant ◽  
Chlorophyll Level ◽  
Lateral Branches ◽  
Mapping Populations

AbstractChlorophyll is a tetrapyrrole metabolite essential for photosynthesis in plants. The oil yellow1 (oy1) gene of maize encodes subunit I of Magnesium chelatase, the enzyme catalyzing the first committed step of chlorophyll biosynthesis. A range of chlorophyll contents and net CO2 assimilation rates can be achieved in maize by combining a semi-dominant mutant allele, Oy1-N1989, and cis-regulatory alleles encoded by the Mo17 inbred called very oil yellow1 (vey1). We previously demonstrated that these allelic interactions can delay reproductive maturity. In this study, we demonstrate that multiple gross morphological traits respond to a reduction in chlorophyll. We found that stalk width, number of lateral branches (tillers), and branching of the inflorescence decline with a decrease in chlorophyll level. Chlorophyll variation suppressed tillering in multiple maize mutants including teosinte branched1, grassy tiller1, and Tillering1 as well as the tiller number1 QTL responsible for tillering in many sweet corn varieties. In contrast to these traits, plant height showed a non-linear response to chlorophyll levels. Weak suppression of Oy1-N1989 by vey1B73 resulted in a significant increase in mutant plant height. This was true in multiple mapping populations, isogenic inbreds, and hybrid backgrounds. Enhancement of the Oy1-N1989 mutants by the vey1Mo17 allele reduced chlorophyll contents and plant height in mapping populations and isogenic inbred background. We demonstrate that the effects of reduced chlorophyll content on plant growth and development are complex and that the genetic relationship depends on the trait. We propose that growth control for branching and architecture are downstream of energy balance sensing.

scholarly journals Interaction Between Induced and Natural Variation at oil yellow1 Delays Reproductive Maturity in Maize

2019 ◽  
Vol 10 (2) ◽  
pp. 797-810
Author(s):  
Rajdeep S. Khangura ◽  
Bala P. Venkata ◽  
Sandeep R. Marla ◽  
Michael V. Mickelbart ◽  
Singha Dhungana ◽  
...  
Keyword(s):  
Flowering Time ◽  
Chlorophyll Content ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Reproductive Maturity ◽  
Magnesium Chelatase ◽  
Chlorophyll Contents ◽  
Delayed Flowering ◽  
Mapping Populations ◽  
Expression Polymorphism

We previously demonstrated that maize (Zea mays) locus very oil yellow1 (vey1) encodes a putative cis-regulatory expression polymorphism at the magnesium chelatase subunit I gene (aka oil yellow1) that strongly modifies the chlorophyll content of the semi-dominant Oy1-N1989 mutants. The vey1 allele of Mo17 inbred line reduces chlorophyll content in the mutants leading to reduced photosynthetic output. Oy1-N1989 mutants in B73 reached reproductive maturity four days later than wild-type siblings. Enhancement of Oy1-N1989 by the Mo17 allele at the vey1 QTL delayed maturity further, resulting in detection of a flowering time QTL in two bi-parental mapping populations crossed to Oy1-N1989. The near isogenic lines of B73 harboring the vey1 allele from Mo17 delayed flowering of Oy1-N1989 mutants by twelve days. Just as previously observed for chlorophyll content, vey1 had no effect on reproductive maturity in the absence of the Oy1-N1989 allele. Loss of chlorophyll biosynthesis in Oy1-N1989 mutants and enhancement by vey1 reduced CO2 assimilation. We attempted to separate the effects of photosynthesis on the induction of flowering from a possible impact of chlorophyll metabolites and retrograde signaling by manually reducing leaf area. Removal of leaves, independent of the Oy1-N1989 mutant, delayed flowering but surprisingly reduced chlorophyll contents of emerging leaves. Thus, defoliation did not completely separate the identity of the signal(s) that regulates flowering time from changes in chlorophyll content in the foliage. These findings illustrate the necessity to explore the linkage between metabolism and the mechanisms that connect it to flowering time regulation.

scholarly journals Interaction between induced and natural variation atoil yellow1delays reproductive maturity in maize

2019 ◽  
Author(s):  
Rajdeep S. Khangura ◽  
Bala P. Venkata ◽  
Sandeep R. Marla ◽  
Michael V. Mickelbart ◽  
Singha Dhungana ◽  
...  
Keyword(s):  
Flowering Time ◽  
Chlorophyll Content ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Reproductive Maturity ◽  
Magnesium Chelatase ◽  
Chlorophyll Contents ◽  
Delayed Flowering ◽  
Mapping Populations ◽  
Expression Polymorphism

AbstractWe previously demonstrated that maize (Zea mays) locusvery oil yellow1 (vey1)encodes a putative cis-regulatory expression polymorphism at the magnesium chelatase subunit I gene (akaoil yellow1) that strongly modifies the chlorophyll content of the semi-dominantOy1-N1989mutants. Thevey1allele of Mo17 inbred line reduces chlorophyll content in the mutants leading to reduced photosynthetic output.Oy1-N1989mutants in B73 reached reproductive maturity four days later than wild-type siblings. Enhancement ofOy1-N1989by the Mo17 allele at thevey1QTL delayed maturity further, resulting in detection of a flowering time QTL in two bi-parental mapping populations crossed toOy1-N1989. The near isogenic lines of B73 harboring thevey1allele from Mo17 delayed flowering ofOy1-N1989mutants by twelve days. Just as previously observed for chlorophyll content,vey1had no effect on reproductive maturity in the absence of theOy1-N1989allele. Loss of chlorophyll biosynthesis inOy1-N1989mutants and enhancement byvey1reduced CO2assimilation. We attempted to separate the effects of photosynthesis on the induction of flowering from a possible impact of chlorophyll metabolites and retrograde signaling by manually reducing leaf area. Removal of leaves, independent of theOy1-N1989mutant, delayed flowering but surprisingly reduced chlorophyll contents of emerging leaves. Thus, defoliation did not completely separate the identity of the signal(s) that regulates flowering time from changes in chlorophyll content in the foliage. These findings illustrate the necessity to explore the linkage between metabolism and the mechanisms that connect it to flowering time regulation.

scholarly journals Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane

2021 ◽  
Vol 3 ◽  
Author(s):  
Ayman Eid ◽  
Chakravarthi Mohan ◽  
Sara Sanchez ◽  
Duoduo Wang ◽  
Fredy Altpeter
Keyword(s):  
Genome Editing ◽  
Chlorophyll Biosynthesis ◽  
Crop Improvement ◽  
Targeted Mutagenesis ◽  
Genetic Redundancy ◽  
Multiple Alleles ◽  
Magnesium Chelatase ◽  
Chlorophyll Contents ◽  
Key Enzyme ◽  
High Level

Genome editing with sequence-specific nucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), is revolutionizing crop improvement. Developing efficient genome-editing protocols for highly polyploid crops, including sugarcane (x = 10–13), remains challenging due to the high level of genetic redundancy in these plants. Here, we report the efficient multiallelic editing of magnesium chelatase subunit I (MgCh) in sugarcane. Magnesium chelatase is a key enzyme for chlorophyll biosynthesis. CRISPR/Cas9-mediated targeted co-mutagenesis of 49 copies/alleles of magnesium chelatase was confirmed via Sanger sequencing of cloned PCR amplicons. This resulted in severely reduced chlorophyll contents, which was scorable at the time of plant regeneration in the tissue culture. Heat treatment following the delivery of genome editing reagents elevated the editing frequency 2-fold and drastically promoted co-editing of multiple alleles, which proved necessary to create a phenotype that was visibly distinguishable from the wild type. Despite their yellow leaf color, the edited plants were established well in the soil and did not show noticeable growth retardation. This approach will facilitate the establishment of genome editing protocols for recalcitrant crops and support further optimization, including the evaluation of alternative RNA-guided nucleases to overcome the limitations of the protospacer adjacent motif (PAM) site or to develop novel delivery strategies for genome editing reagents.

scholarly journals THE EFFECT OF LIQUID ORGANIC FERTILIZER CONCENTRATION ON GROWTH AND RESULS OF VARIOUS SWEET CORN LINES IN MARGINAL LAND USING A DRIP IRRIGATION SYSTEM

Agrivet ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 8
Author(s):  
Bambang Supriyanta ◽  
Oktavia Sarhesti Padmini ◽  
Danar Wicaksono ◽  
Kundarto Kundarto
Keyword(s):  
Plant Height ◽  
Drip Irrigation ◽  
Sweet Corn ◽  
Agricultural Research ◽  
Irrigation System ◽  
Organic Fertilizer ◽  
Leaf Length ◽  
Marginal Land ◽  
High Porosity ◽  
Drip Irrigation System

Utilization of the potential of marginal land with adequate water and nutrients is an important factor in increasing corn production. However, high porosity levels and low nutrient levels cause growth and production of corn is not optimal. The aim of this research is to obtain an irrigation technique combined with efficient fertilization in the sweet corn cultivation system in marginal land. The field research was carried out at Faculty of Agricultural Research Wedomartani, Depok Sleman Yogyakarta. The study was conducted from September 2019 to February 2020. The research aims to get best sweet corn line at various concentrations of liquid organic fertilizer in marginal land with a drip irrigation system.The research was arranged in a completely randomized design (CRD) with 3 replications. The first factor is sweet corn lines, which consists of 9 levels, namely, G1 (5042B), G2 (KD-11), G3 (KD-13), G4 (SB-11), G5 (SB-13), G6 (SB-14), G7 (SB-16), G8 (SB-21), and G9 (SB-22). While the second factor is the irrigation system combined with fertilization (fertigation) which consists of 3 levels, namely the drip irrigation system with a concentration of liquid supplement fertilizer 2 cc / L, 3 cc / L and 4 cc / L. The results showed that the drip fertigation system with a concentration of POC 3 cc / L was able to give a better effect on plant height and leaf length in sweet corn. SB / 1-3 lines was the best lines as prospective parents for making hybrid lines for plant height, leaf length, number of leaves and cob length.Keywords: lines, liquid organic fertilizer, sweet corn, marginal land, irrigation system

scholarly journals Pertumbuhan Vegetatif dan Kadar Gula Biji Jagung Manis (Zea mays saccharata, Sturt) di Pekanbaru

2018 ◽  
Vol 13 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Surtinah Surtinah ◽  
Seprita Lidar
Keyword(s):  
Plant Height ◽  
Sweet Corn ◽  
Polynomial Regression ◽  
Sugar Content ◽  
Leaf Length ◽  
Leaf Width ◽  
Design Environment ◽  
Corn Seed ◽  
Randomized Design ◽  
Corn Kernels

Research conducted an experiment using a completely randomized design environment with four replications, and the design of treatment used is six varieties of sweet corn. Analysis of data using polynomial regression, the parameters measured were plant height, leaf number, leaf length, leaf width, and sugar beans, followed by analyzing the relationship between the growth of plants with a sugar content of sweet corn kernels. The results showed that leaf width gives a weak relationship to the sugar content of sweet corn seed, and leaf length, number of leaves and plant height had a close relationship to the sugar content of sweet corn kernels.

scholarly journals Effects of Exogenous 6-Benzyladenine on Dwarfing, Shoot Branching, and Yield of Tea Plant (Camellia sinensis)

HortScience ◽  
2018 ◽  
Vol 53 (5) ◽  
pp. 651-655 ◽  
Author(s):  
Liping Zhang ◽  
Chen Shen ◽  
Jipeng Wei ◽  
Wenyan Han
Keyword(s):  
Plant Height ◽  
Camellia Sinensis ◽  
Photosynthetic Rate ◽  
Tea Plant ◽  
Shoot Branching ◽  
Tea Plants ◽  
Lateral Branches ◽  
Spring Yield

6-Benzyladenine (6-BA) is a safe and efficient cytokinin. The adult tea plants of the cv. Longjing 43 were used in this study. The foliar portion of tea bushes were sprayed with different concentrations (50, 100, 200, or 400 mg·L−1) of 6-BA after heavy pruning, when three to four leaves grew out in late May. The effects of 6-BA application on the growth of the new shoots and lateral branches were quantified. After 5 months, treatments with 50, 100, 200, or 400 mg·L−1 6-BA suppressed plant height by 11.0%, 18.0%, 21.0%, or 22.0%, respectively; 6-BA at 100, 200, or 400 mg·L−1 decreased the number of lateral branches by 20.0%, 23.0%, or 18.0%, respectively. Meanwhile, treatments with 50, 200, or 400 mg·L−1 6-BA increased the length of lateral branches by 38.0%, 79.0%, or 81.0% respectively; 200 mg·L−1 6-BA increased the diameter of lateral branches by 8.0%. In addition, after 2 months, 50 or 200 mg·L−1 6-BA did not significantly affect the growth of functional leaves, 50, 100, or 200 mg·L−1 6-BA did not significantly affect photosynthetic rate (Pn) as compared with the control. Furthermore, 200 or 400 mg·L−1 6-BA significantly increased spring tea yield by 28.9% or 13.3%, respectively as compared with the control. In conclusion, 6-BA at the four concentrations promoted dwarfing and the formation of productive lateral branches and increased the spring yield, and 200 mg·L−1 6-BA exerted the best comprehensive effect.

Management of Liquid Organic Waste from Palm Oil Plant and Compost Cow to the Growth and Production of Sweet Corn (Zea mays saccharata Sturt)

2020 ◽  
pp. 1-8
Author(s):  
Tharmizi Hakim
Keyword(s):  
Zea Mays ◽  
Plant Height ◽  
Palm Oil ◽  
Sweet Corn ◽  
Raw Materials ◽  
Block Design ◽  
Palm Oil Mill Effluent ◽  
Cow Dung ◽  
Palm Oil Mill ◽  
Mill Waste

The growth and production of sweet corn (Zea mays saccharata Sturt) are strongly influenced by good genetics and environmental factors, including how to cultivate the soil so that the availability of nutrients is quite balanced. The long-term goal of this research is to determine the effectiveness of a combination of palm oil mill effluent (LCPKS) and cow dung waste (LKTS) with EM4 bio-activator applied to sweet corn (Zea mays saccharata Sturt). This study used a factorial randomized block design (RBD) consisting of 2 factors with 12 treatment combinations and 3 replications to obtain 36 research plots. The factors studied are the formulation factor of a mixture of palm oil mill effluent with cow dung waste, which is denoted by (A) consisting of A1 = 70% palm oil mill waste and 30% cow dung, A2 = 50% effluent palm oil mill and 50% cow dung waste, A3 = 30% palm oil mill waste and 70% cow dung waste. The dose factor with the symbol (D) consists of, D0 = control, D1 = 1.5 kg per plot, D2 = 3 kg per plot, D3 = 4.5 kg per plot. The parameters observed were plant height (cm), biomass samples (grams), sample production (grams) and plot production (kg). The results showed that different waste mix formulations and administration of different doses did not significantly influence plant height parameters because compost maturity must be assessed by measuring two or more compost parameters to prevent phytotoxicity. Therefore, quality assurance must be carried out on compost to determine the factors that cause phytotoxicity and also research must be intensified in determining lines that will be able to degrade waste faster, more efficiently and also produce non-toxic compost that mixes the soil produced in plant growth. In addition, care must be taken in selecting raw materials for composting. The results showed that the mixed waste formulations significantly affected the biomass of the sample, where the highest average was obtained in processing A3 (30% of palm oil mill waste and 70% of cow dung waste. The research results also showed that dosing had a very significant effect on biomass parameter sample, sample production and plot production where the highest average is in the D3 treatment (4.5 kg per plot).

Characterization of the magnesium chelatase from Thermosynechococcus elongatus

2013 ◽  
Vol 457 (1) ◽  
pp. 163-170 ◽  
Author(s):  
Nathan B. P. Adams ◽  
Christopher J. Marklew ◽  
Amanda A. Brindley ◽  
C. Neil Hunter ◽  
James D. Reid
Keyword(s):  
Chlorophyll Biosynthesis ◽  
Thermostable Enzyme ◽  
Enzyme Complex ◽  
Magnesium Chelatase

Magnesium chelatase is the ‘gatekeeper’ multi-subunit enzyme complex that initiates chlorophyll biosynthesis; we present the first characterization of an active thermostable enzyme complex and we use hybrid mesophilic/thermophilic chelatase complexes to reveal that Mg2+ co-operativity resides in the ChlD subunit.

Effect of laser priming on canola yield and its components under salt stress

2012 ◽  
Vol 26 (1) ◽  
pp. 45-51 ◽  
Author(s):  
S. Mohammadi ◽  
F. Shekari ◽  
R. Fotovat ◽  
A. Darudi
Keyword(s):  
Salt Stress ◽  
Plant Height ◽  
Correlation Coefficients ◽  
Yield Increase ◽  
Effect On Yield ◽  
Lateral Branches ◽  
Pod Length ◽  
Positive Effect ◽  
Pod Number ◽  
Number Of Seeds

Effect of laser priming on canola yield and its components under salt stressThe effect of laser priming at different irradiation times on canola yield and its components under saline conditions were investigated. The results showed that laser priming had a positive effect on yield and its components and caused yield increase under saline conditions. Increase in salt levels had a negative and significant effect on seed yield, number of seeds per pod, number of pod per plant, pod length and plant height. The results showed that 45-min laser priming had the strongest effect on yield and yield components and reduced significantly the adverse effects of salinity. By contrast, laser radiation applied for 60 and 75 min, resulted in a dramatic decrease in yield and its components. Correlation coefficients between the attributes showed that canola yield had a positive and significant correlation with plant height, number of seeds, pod per main branch and lateral branches, length of pod and number of lateral branches. Effects of laser and salinity were significant on lateral branch pod length but not on main branch pods.

Sweet Corn Hybrid Sensitivity to Clopyralid

2005 ◽  
Vol 19 (2) ◽  
pp. 342-345 ◽  
Author(s):  
Nader Soltani ◽  
Shane Diebold ◽  
Darren E. Robinson ◽  
Peter H. Sikkema
Keyword(s):  
Plant Height ◽  
Detrimental Effect ◽  
Field Experiments ◽  
Sweet Corn ◽  
Yield Response ◽  
Limited Information ◽  
Marketable Yield ◽  
Corn Hybrids ◽  
Growing Conditions ◽  
Corn Hybrid

Limited information exists on sweet corn tolerance to postemergence (POST) applications of clopyralid under Ontario growing conditions. Eight sweet corn hybrids were evaluated for tolerance to clopyralid in three field experiments conducted in 2001 and 2002 in Ontario. Clopyralid was applied POST at 200 and 400 g ai/ha, the proposed and twice the proposed registered rate for use in sweet corn in Ontario. Sweet corn response to clopyralid did not vary among the hybrids tested. In 2001, visual injury among hybrids 7 d after treatment (DAT) with clopyralid at 400 g/ha was less than 3%. Subsequent visual injury evaluations at 14 and 28 DAT showed no differences among sweet corn hybrids at either rate of clopyralid evaluated. The application of clopyralid at 200 and 400 g/ ha had no detrimental effect on plant height or marketable yield of any of the eight sweet corn hybrids. On the basis of visual injury, height, and marketable yield response ‘Calico Belle’, ‘CNS 710’, ‘DelMonte 2038’, ‘GG 222’, ‘GG 246’, ‘GH 2684’, ‘Reveille’, and ‘Rival’ are all tolerant to the POST application of clopyralid.

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