scholarly journals Characterization of Vibrio cholerae isolates from freshwater sources in northwest Ohio

2020 ◽  
Author(s):  
Judy Daboul ◽  
Logan Weghorst ◽  
Cara DeAngelis ◽  
Sarah Plecha ◽  
Jessica Saul-McBeth ◽  
...  

AbstractVibrio cholerae is a natural inhabitant of aquatic ecosystems worldwide, typically residing in coastal or brackish water. While more than 200 serogroups have been identified, only serogroups O1 and O139 have been associated with epidemic cholera. However, infections other than cholera can be caused by nonepidemic, non-O1/non-O139 V. cholerae strains, including gastroenteritis and extraintestinal infections. While V. cholerae can also survive in freshwater, that is typically only observed in regions of the world where cholera is endemic. We recently isolated V. cholerae from several locations in lakes and rivers in northwest Ohio. These isolates were all found to be non-O1/non-O139 V. cholerae strains, that would not cause cholera. However, these isolates contained a variety of virulence genes, including ctxA, rtxA, rtxC, hlyA, and ompU. Therefore, it is possible that some of these isolates have the potential to cause gastroenteritis or other infections in humans. We also investigated the relative motility of the isolates and their ability to form biofilms as this is important for V. cholerae survival in the environment. We identified one isolate that forms very robust biofilms, up to 4x that of our laboratory control strains. Finally, we investigated the susceptibility of these isolates to a panel of antibiotics. We found that many of the isolates showed decreased susceptibility to some of the antibiotics tested, which could be of concern. While we do not know if these isolates are pathogenic to humans, increased surveillance to better understand the public health risk to the local community should be considered.ImportanceThis study found that Vibrio cholerae belonging to non-O1/non-O139 serogroups is present in freshwater lakes and rivers in northwest Ohio. Although non-O1/non-O139 V. cholerae strains generally do not produce cholera toxin, and thus do not cause epidemic cholera, they often contain other virulence factors that may contribute to pathogenicity. In fact, we found that these local isolates contained an assortment of potential virulence genes. We also found that some of the isolates showed antibiotic resistance. While we do not currently know if the local V. cholerae strains are capable of causing disease in humans, increased monitoring for V. cholerae in the region is warranted in the interest of public health.

Author(s):  
Khaled Asfour

In Vitruvius’ treatise, what makes good architecture is its ability to communicate to the public particular messages that reflects the program of the building with spaces and components arranged in an orderly way. According to Vitruvius these messages when acknowledges by the public the building posses strong character. This research discusses this idea by reflecting on the 1895 competition of the Egyptian Museum project. Marcel Dourgnon, the French architect of the winning scheme, showed profound understanding of character resulting in a building that had positive vibe with the local community.  Today Vitruvius’ idea is still living with us. Norman Foster succeeded in upgrading the British Museum in a way that addressed all cultures of the world through his grand atrium design.  Similarly, Emad Farid and Ramez Azmy revived the presence of the Egyptian Museum in public cognition.  Spatial experience that evokes similar perceptions to all its visitors is a timeless piece that transcends cultural boundaries.


Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 735
Author(s):  
Julie Dormoy ◽  
Marc-Olivier Vuillemin ◽  
Silvia Rossi ◽  
Jean-Marc Boivin ◽  
Julie Guillet

Background: Antibiotic resistance is a global health crisis. The aim of this study was to explore dentists’ perceptions of antibiotic resistance. Methods: A qualitative method was used. Seventeen dentists practising in the Nancy (Lorraine, France) region were surveyed. They were general practitioners or specialised in oral surgery, implantology, or periodontology. The practitioners took part in semi-structured interviews between September 2019 and July 2020. All of the interviews were transcribed in full and analysed thematically. Results: Four major themes have been selected: attitudes of the dentists in regard to the guidelines, clinical factors that influence prescriptions, non-clinical factors that influence prescriptions, and the perception of antibiotic resistance. The dentists stated that they were very concerned regarding the public health issue of antibiotic resistance. However, they often prescribe according to their own interests and habits rather than according to the relevant guidelines. Conclusions: Although dentists are generally well aware of antibiotic resistance, they often do not adequately appreciate the link between their prescribing habits and the phenomenon of antibiotic resistance. Regular updating of practitioners’ knowledge in this regard is necessary, but patients and the general public should also be made more aware of the issue.


2014 ◽  
Vol 971-973 ◽  
pp. 2350-2353
Author(s):  
Ai Yun Sun ◽  
Xi Yang Ding

martial arts through the promotion of conditions , difficulties and countermeasures analysis of the system, that China should be based on public health and martial arts fighting two clues to promote athletics , martial arts part of the refining and development of the " elite sports " and select wide popularity part , to promote the realization of the true sense of the public , in order to improve business operations and direction of development to promote social and economic development and to meet the needs of people in sports consumption level . In other words, watching athletics , martial arts fitness and economic integration of the three organic constituted martial arts through the promotion of the premise, but also the power of martial arts to the world .


Author(s):  
Alok Tiwari

ABSTRACTCOVID-19 epidemic is declared as the public health emergency of international concern by the World Health Organisation in the second week of March 2020. This disease originated from China in December 2019 has already caused havoc around the world, including India. The first case in India was reported on 30th January 2020, with the cases crossing 6000 on the day paper was written. Complete lockdown of the nation for 21 days and immediate isolation of infected cases are the proactive steps taken by the authorities. For a better understanding of the evolution of COVID-19 in the country, Susceptible-Infectious-Quarantined-Recovered (SIQR) model is used in this paper. It is predicted that actual infectious population is ten times the reported positive case (quarantined) in the country. Also, a single case can infect 1.55 more individuals of the population. Epidemic doubling time is estimated to be around 4.1 days. All indicators are compared with Brazil and Italy as well. SIQR model has also predicted that India will see the peak with 22,000 active cases during the last week of April followed by reduction in active cases. It may take complete July for India to get over with COVID-19.


F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 662
Author(s):  
Tarig A. Gamar ◽  
Hassan H. Musa ◽  
Hisham N. Altayb ◽  
Mohamed H. Mohamed ◽  
Adam D. Abakar

Background: Hookworms infect the intestines, cause an itchy rash, respiratory and gastrointestinal problems, and eventually iron deficiency (anaemia) due to the ongoing loss of blood. The objectives of this study were to assess the prevalence and molecular characterization of hookworms isolated from food handlers attending the Public Health Laboratories in Khartoum state, Sudan, for annual check-ups, and to assess the efficiency of PCR as molecular probe for hookworm infection. Methods: A total of 350 foods handlers’ participant's stool samples who were not suspected to be infected with hookworms were studied. Conventional methods were applied to make an early diagnosis. Stool samples were collected from public health laboratories (the public health lab in the Medical Commission) of Khartoum State; Omdurman locality, Khartoum North locality and Khartoum locality between October 2016 and April 2017. Specific identification was made by PCR on specimens identified as positive by Baermann’s technique, which were then sequence and genotyped Results: The prevalence of hookworms in the stool samples of food-handlers was 1.43%. One larval specimen recovered by Baermann’s technique was confirmed to be Necator americanus by PCR. PCR also confirmed that Necator americanus was the common species isolated from four further specimens. The results of DNA sequencing for Necator americanus were deposited in NCBI GenBank under the following accession numbers: sample 91, MH035824; sample 92, MH035825; sample 294, MH035826; and sample 319 MH035827. Conclusion: PCR was found to be effective for confirmation of the diagnosis of hookworm infection and can aid the clinician in initiating prompt and appropriate antiparasite therapy.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ante Mandarić ◽  
Goran Matijević

The epidemic of the disease COVID-19, in Požeština in relation to China, where it originated in other parts of Croatia, appeared somewhat later, while Požega-Slavonia County in terms of total share in relation to other counties in Croatia remained relatively well , 16th place, out of a total of 20 counties, ie a smaller number of patients was recorded. In the conditions of public health danger to the health and lives of people with expressed uncertainty, citizens around the world were flooded with numerous information, about the disease, ways of prevention, treatment that at one point threatened to turn into an infodemia, as warned by the WHO. The importance of crisis communication in such conditions is of great importance, and how governments and headquarters communicate messages about the crisis to the public, which is discussed in the first parts of the paper and points out several inconsistencies and illogicalities in the actions of the state headquarters. prohibition and permission to make recommendations contrary to the epidemiologist’s recommendations. But more important than the recommendations of headquarters and governments, today are the recommendations and news transmitted by digital media, and especially the local ones that bring news and recommendations for the area where we live. Therefore, the aim of this paper was to investigate in the central part the significance of the local 034 Portal in the Corona crisis, and its monitoring of the crisis and its impact on the public. Research through several segments, it was found that the portal maintained the level of reporting on regular events and adjusted reporting on the Crown to the conditions and situation in the county, not leading to sensationalism, concern, fear, but was a carrier of preventive activities and a good ally in the fight. against the epidemic, that is, he followed the guidelines for informing the WHO and did not contribute to the creation of an infodemia.


Author(s):  
Timothy H. Holtz ◽  
Leighton Jessica ◽  
Sharon Balter ◽  
Don Weiss ◽  
Susan Blank ◽  
...  

Author(s):  
Teodora Kiryakova-Dineva ◽  
Ruska Bozhkova

At a time of the global health pandemic, the most affected areas are economy and social life. Along with the practical limitations of travel, regarding personal security reasons and the objective risks for the environment, the world of tourism has changed. However, under the circumstances, some small accommodation units have managed to survive, like the Seamen between Scylla and Charybdis – the mythical situation. The purpose of this chapter is to delve into the public health risk environment for Bulgarian SMEs in tourism (guest houses and family hotels) during the COVID-19 pandemic. The extent of the analysis includes hotels and guest houses in the south-western part of Bulgaria that managed to keep operating despite the global pandemic situation.


Pained ◽  
2020 ◽  
pp. 29-30
Author(s):  
Michael D. Stein ◽  
Sandro Galea

This chapter addresses how racism presents a clear threat to the health of populations. In 2018, President Donald Trump made racist comments toward countries with predominantly nonwhite populations. Why did the president’s racism matter for the health of the public? To answer this question, one needs to understand where health comes from. Health is the product of the social, economic, and cultural context in which people live. This context is also shaped by social norms that do much to determine people’s behaviors and their consequences. Changing these norms can produce both positive and negative health effects. On the positive side, changing norms can promote health, by making unacceptable unhealthy conditions and behaviors that were once common, even celebrated. On the negative side, changing norms for the worse can empower elements of hate in society. When a president promotes hate, it shifts norms, suggesting that hate does in fact have a place in the country and the world. This opens the door to more hate crimes, more exclusion of minority groups from salutary resources, and little to no effort to address racial health gaps.


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