scholarly journals Ear wound healing in MRL/MpJ mice is associated with gut microbiome composition and is transferable to non-healer mice via microbiome transplantation

2021 ◽  
Author(s):  
Cassandra Velasco ◽  
Christopher Dunn ◽  
Cassandra Sturdy ◽  
Vladislav Izda ◽  
Jake Martin ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Regenerative Therapy ◽  
Repair Capacity ◽  
Gram Negative ◽  
16S Sequencing ◽  
Future Studies ◽  
Microbiome Composition ◽  
Sex Effects ◽  
Gram Negative Organisms ◽  
Better Than

AbstractObjectiveAdult cartilage has limited repair capacity. MRL/MpJ mice, by contrast, are capable of spontaneously healing ear punctures. This study was undertaken to characterize microbiome differences between healer and nonhealer mice and to evaluate microbiome transplantation as a novel regenerative therapy.MethodsWe transplanted C57BL/6J mice with MRL/MpJ cecal contents in mice at weaning and as adults (n=57) and measured earhole closure 4 weeks after a 2.0mm punch and compared to vehicle-transplanted MRL and B6 (n=25) and B6-transplanted MRL (n=20) mice. Sex effects, timing of transplant relative to earpunch, and transgenerational heritability were evaluated. In a subset (n=58), cecal microbiomes were profiled by 16S sequencing and compared to earhole closure rates. Microbial metagenomes were imputed using PICRUSt.ResultsTransplantation of B6 mice with MRL microbiota, either in weanlings or adults, improved earhole closure rates. Transplantation prior to ear punch was associated with the greatest earhole closure. Offspring of transplanted mice healed better than controls. Several microbiome clades were correlated with healing, including Firmicutes, Lactobacillales, and Verrucomicrobia. Gram-negative organisms were reduced. Females of all groups tended to heal better than males, female microbiota resembled MRL mice.ConclusionIn this study, we found an association between the microbiome and tissue regeneration in MRL mice and demonstrate that this trait can be transferred to nonhealer mice via microbiome transplantation. We identified several microbiome clades associated with healing. Future studies should evaluate the mechanisms underlying these findings and confirm our results in murine OA.

scholarly journals Ear wound healing in MRL/MpJ mice is associated with gut microbiome composition and is transferable to non-healer mice via microbiome transplantation

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0248322
Author(s):  
Cassandra Velasco ◽  
Christopher Dunn ◽  
Cassandra Sturdy ◽  
Vladislav Izda ◽  
Jake Martin ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Gut Microbiome ◽  
Vehicle Control ◽  
Repair Capacity ◽  
16S Sequencing ◽  
Microbiome Composition ◽  
Sex Effects ◽  
Elastic Cartilage ◽  
Better Than ◽  
Hole Closure

Objective Adult elastic cartilage has limited repair capacity. MRL/MpJ (MRL) mice, by contrast, are capable of spontaneously healing ear punctures. This study was undertaken to characterize microbiome differences between healer and non-healer mice and to evaluate whether this healing phenotype can be transferred via gut microbiome transplantation. Methods We orally transplanted C57BL/6J (B6) mice with MRL/MpJ cecal contents at weaning and as adults (n = 57) and measured ear hole closure 4 weeks after a 2.0mm punch and compared to vehicle-transplanted MRL and B6 (n = 25) and B6-transplanted MRL (n = 20) mice. Sex effects, timing of transplant relative to earpunch, and transgenerational heritability were evaluated. In a subset (n = 58), cecal microbiomes were profiled by 16S sequencing and compared to ear hole closure. Microbial metagenomes were imputed using PICRUSt. Results Transplantation of B6 mice with MRL microbiota, either in weanlings or adults, improved ear hole closure. B6-vehicle mice healed ear hole punches poorly (0.25±0.03mm, mm ear hole healing 4 weeks after a 2mm ear hole punch [2.0mm—final ear hole size], mean±SEM), whereas MRL-vehicle mice healed well (1.4±0.1mm). MRL-transplanted B6 mice healed roughly three times as well as B6-vehicle mice, and half as well as MRL-vehicle mice (0.74±0.05mm, P = 6.9E-10 vs. B6-vehicle, P = 5.2E-12 vs. MRL-vehicle). Transplantation of MRL mice with B6 cecal material did not reduce MRL healing (B6-transplanted MRL 1.3±0.1 vs. MRL-vehicle 1.4±0.1, p = 0.36). Transplantation prior to ear punch was associated with the greatest ear hole closure. Offspring of transplanted mice healed significantly better than non-transplanted control mice (offspring:0.63±0.03mm, mean±SEM vs. B6-vehicle control:0.25±0.03mm, n = 39 offspring, P = 4.6E-11). Several microbiome clades were correlated with healing, including Firmicutes (R = 0.84, P = 8.0E-7), Lactobacillales (R = 0.65, P = 1.1E-3), and Verrucomicrobia (R = -0.80, P = 9.2E-6). Females of all groups tended to heal better than males (B6-vehicle P = 0.059, MRL-transplanted B6 P = 0.096, offspring of MRL-transplanted B6 P = 0.0038, B6-transplanted MRL P = 1.6E-6, MRL-vehicle P = 0.0031). Many clades characteristic of female mouse cecal microbiota vs. males were the same as clades characteristic of MRL and MRL-transplanted B6 mice vs. B6 controls, including including increases in Clostridia and reductions in Verrucomicrobia in female mice. Conclusion In this study, we found an association between the microbiome and tissue regeneration in MRL mice and demonstrate that this trait can be transferred to non-healer mice via microbiome transplantation. We identified several microbiome clades associated with healing.

scholarly journals Comparison of Three Dimensional Test and Double Disc Synergy Test for detection of Extended Spectrum ?-Lactamase (ESBL) producing Gram negative bacteria

Pulse ◽  
2014 ◽  
Vol 6 (1-2) ◽  
pp. 12-19
Author(s):  
SM Biswas ◽  
MRA Mia ◽  
N Ara ◽  
M Ibrahim ◽  
TA Nasir ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Gram Negative Bacteria ◽  
Esbl Producer ◽  
Gram Negative ◽  
Wound Swab ◽  
Extended Spectrum ◽  
Synergy Test ◽  
Gram Negative Organisms ◽  
Better Than

Extended spectrum ?-lactamase (ESBLs), produced by Gram negative organisms, are enzymes capable of hydrolyzing extended spectrum cephalosporins, penicillins and monobactam but inactive against cephamycin and imipenem. Detection of some ESBL strains are missed by Double disc synergy test but detected by three dimensional test. So this study was carried out to see the rate of ESBL producer by three dimensional test and double disc synergy test among Gram negative bacteria. Total 110 Gram negative isolates were studied, among them 30 were wound swab isolates and 80 were laboratory isolates, of which 88(80%) were ESBL producer. Three dimensional tests detected ESBL in 88 (80%) strain whereas 66.36% strains detected by double disc synergy test, so 15 (13.63%) isolates were missed by Double disc synergy test. In this study among the 30 wound swab isolates Three dimensional test detected 26 (86.67%) whereas 14 (46.67) were detected by double disc synergy test and similarly among the 80 laboratory isolates, three dimensional test detected 62 (77.5%) and double disc synergy test detected 59 (73.75%). So, the three dimensional test has been found to be better than Double disc synergy test in the detection of ESBLs. ESBL producing organisms are resistance to most of the antibiotics but 100% sensitive to Imipenem. DOI: http://dx.doi.org/10.3329/pulse.v6i1-2.20328 Pulse Vol.6 January-December 2013 p.12-19

Some of Frontiers Education Articles: Around First to Eleven December 2020

2020 ◽  
Author(s):  
thobias sarbunan
Keyword(s):  
Knowledge Creation ◽  
Publishing House ◽  
General Point ◽  
Human Race ◽  
Future Studies ◽  
Long Period ◽  
Research And Innovation ◽  
New Information ◽  
Scientific Ideas ◽  
Better Than

The research pathway is also an important point to lead researchers in creating and enriching knowledge from a fresh viewpoint, as wellas development for the human race. The frontier is the publishing house of a publication that has established information along with the'other agent' of knowledge around the globe. As a result, one of the sub-journals of this publication was education, expanded awarenesstime by time, by new information on innovation in science and technology. In the meantime, the pandemic, better than the science society,has alerted to the current developments in science aimed at strengthening and gaining some insight and awareness of how to maintainthe 'mode of knowledge creation'. So, through this discussion of the current edition of Frontier Education Journals, I thought that thisdiscussion theoretically involved encouragement and advancement in the middle of the pandemic, also influenced from a general point ofview, here as roadmap or step-stone for all research and innovation researchers. On the basis of the discussion in general, I saw that theroad map of the topic of frontier education is in significance to all branches of expertise of education. I agree that knowledge developmenttime-by-time needs to be reflected-analysed-synthesized-adopted or adapted-also developed for the purpose of education in addition tolearning from a general viewpoint. Note, knowledge is never-never sleeping tight, but it still evolves and progresses a long period with thenewest scientific ideas-concept-and hypothesis. In the other hand, it is possible that my study would miss a range of weaknesses inliteracy resources as well; but at least, I have sought, through this article, to see the importance of knowledge advancement that can enrichknowledge in the middle of the pandemic and for future studies.

scholarly journals Linearized esculentin-2EM shows pH dependent antibacterial activity with an alkaline optimum

2021 ◽  
Author(s):  
Erum Malik ◽  
David A. Phoenix ◽  
Timothy J. Snape ◽  
Frederick Harris ◽  
Jaipaul Singh ◽  
...  
Keyword(s):  
Helical Structure ◽  
Food Preservation ◽  
Forming Process ◽  
Gram Positive ◽  
Gram Negative ◽  
Bacterial Membranes ◽  
Gram Positive Bacteria ◽  
Alkaline Conditions ◽  
Ph Dependent ◽  
Gram Negative Organisms

AbstractHere the hypothesis that linearized esculentin 2EM (E2EM-lin) from Glandirana emeljanovi possesses pH dependent activity is investigated. The peptide showed weak activity against Gram-negative bacteria (MLCs ≥ 75.0 μM) but potent efficacy towards Gram-positive bacteria (MLCs ≤ 6.25 μM). E2EM-lin adopted an α-helical structure in the presence of bacterial membranes that increased as pH was increased from 6 to 8 (↑ 15.5–26.9%), whilst similar increases in pH enhanced the ability of the peptide to penetrate (↑ 2.3–5.1 mN m−1) and lyse (↑ 15.1–32.5%) these membranes. Theoretical analysis predicted that this membranolytic mechanism involved a tilted segment, that increased along the α-helical long axis of E2EM-lin (1–23) in the N → C direction, with −  < µH > increasing overall from circa − 0.8 to − 0.3. In combination, these data showed that E2EM-lin killed bacteria via novel mechanisms that were enhanced by alkaline conditions and involved the formation of tilted and membranolytic, α-helical structure. The preference of E2EM-lin for Gram-positive bacteria over Gram-negative organisms was primarily driven by the superior ability of phosphatidylglycerol to induce α-helical structure in the peptide as compared to phosphatidylethanolamine. These data were used to generate a novel pore-forming model for the membranolytic activity of E2EM-lin, which would appear to be the first, major reported instance of pH dependent AMPs with alkaline optima using tilted structure to drive a pore-forming process. It is proposed that E2EM-lin has the potential for development to serve purposes ranging from therapeutic usage, such as chronic wound disinfection, to food preservation by killing food spoilage organisms.

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