scholarly journals Fibrin Prestress Due to Platelet Aggregation and Contraction Increases Clot Stiffness

2021 ◽  
Author(s):  
Suyog Jitendra Pathare ◽  
Wilson Eng ◽  
Sang-Joon J Lee ◽  
Anand Ramasubramanian
Keyword(s):  
Platelet Aggregation ◽  
Mechanical Response ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Fibrin Networks ◽  
Custom Made ◽  
Simulation Based ◽  
Blood Clots ◽  
Platelet Aggregates ◽  
Underlying Mechanisms

Efficient haemorrhagic control is attained through the formation of strong and stable blood clots at the site of injury. Although it is known that platelet-driven contraction can dramatically influence clot stiffness, the underlying mechanisms by which platelets assist fibrin networks in resisting external loads are not understood. In this study, we delineate the contribution of platelet-fibrin interactions to clot tensile mechanics using a combination of new mechanical measurements, image analysis, and structural mechanics simulation. Based on uniaxial tensile test data using custom-made microtensometer, and fluorescence microscopy of platelet aggregation and platelet-fibrin interactions, we show that integrin-mediated platelet aggregation and actomyosin-driven platelet contraction synergistically increase the elastic modulus of the clots. We demonstrate that the mechanical and geometric response of an active contraction model of platelet aggregates compacting vicinal fibrin is consistent with the experimental data. The model suggests that platelet contraction induces prestress in fibrin fibres, and increases the effective stiffness in both crosslinked and non-crosslinked clots. Our results provide evidence for fibrin compaction at discrete nodes as a major determinant of mechanical response to applied loads.

scholarly journals Ability of Constitutive Models to Characterize the Temperature Dependence of Rubber Hyperelasticity and to Predict the Stress-Strain Behavior of Filled Rubber under Different Defor Mation States

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 369
Author(s):  
Xintao Fu ◽  
Zepeng Wang ◽  
Lianxiang Ma
Keyword(s):  
Experimental Data ◽  
Temperature Dependence ◽  
Mechanical Response ◽  
Constitutive Models ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Chain Model ◽  
Stress Strain ◽  
Filled Rubber ◽  
Yeoh Model

In this paper, some representative hyperelastic constitutive models of rubber materials were reviewed from the perspectives of molecular chain network statistical mechanics and continuum mechanics. Based on the advantages of existing models, an improved constitutive model was developed, and the stress–strain relationship was derived. Uniaxial tensile tests were performed on two types of filled tire compounds at different temperatures. The physical phenomena related to rubber deformation were analyzed, and the temperature dependence of the mechanical behavior of filled rubber in a larger deformation range (150% strain) was revealed from multiple angles. Based on the experimental data, the ability of several models to describe the stress–strain mechanical response of carbon black filled compound was studied, and the application limitations of some constitutive models were revealed. Combined with the experimental data, the ability of Yeoh model, Ogden model (n = 3), and improved eight-chain model to characterize the temperature dependence was studied, and the laws of temperature dependence of their parameters were revealed. By fitting the uniaxial tensile test data and comparing it with the Yeoh model, the improved eight-chain model was proved to have a better ability to predict the hyperelastic behavior of rubber materials under different deformation states. Finally, the improved eight-chain model was successfully applied to finite element analysis (FEA) and compared with the experimental data. It was found that the improved eight-chain model can accurately describe the stress–strain characteristics of filled rubber.

Design and development of aluminum alloy 6061-T6 pressure vessel liner for aerospace applications: A technical brief

2021 ◽  
pp. 146442072110651
Author(s):  
R Pramod ◽  
N Siva Shanmugam ◽  
C K Krishnadasan ◽  
G Radhakrishnan ◽  
Manu Thomas
Keyword(s):  
Tensile Strength ◽  
Aluminum Alloy ◽  
Weld Metal ◽  
Tensile Test ◽  
Pressure Vessel ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Aluminum Alloy 6061 ◽  
Custom Made ◽  
Alloy 6061

This work mainly focuses on designing a novel aluminum alloy 6061-T6 pressure vessel liner intended for use in launch vehicles. Fabrication of custom-made welding fixtures for the assembly of liner parts, namely two hemispherical domes and end boss, is illustrated. The parts of the liner are joined using the cold metal transfer welding process, and the welding trials are performed to arrive at an optimized parametric range. The metallurgical characterization of weld joint reveals the existence of dendritic structures (equiaxed and columnar). Microhardness of base and weld metal was 70 and 65 HV, respectively. The tensile strength of base and weld metal was 290 and 197 MPa, respectively, yielding a joint efficiency of 68%. Finite-element analysis of a uniaxial tensile test was performed to predict the tensile strength and location of the fracture in base and weld metal. The experimental and predicted tensile test results were found to be in good agreement.

scholarly journals Analysis of human emboli and thrombectomy forces in large-vessel occlusion stroke

2020 ◽  
pp. 1-9 ◽  
Author(s):  
Yang Liu ◽  
Yihao Zheng ◽  
Adithya S. Reddy ◽  
Daniel Gebrezgiabhier ◽  
Evan Davis ◽  
...  
Keyword(s):  
Glass Tube ◽  
Ultimate Tensile Stress ◽  
Material Behavior ◽  
Large Vessel ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Vessel Occlusion ◽  
Cell Percentage ◽  
Uniaxial Tensile Testing ◽  
Custom Made

OBJECTIVEThis study’s purpose was to improve understanding of the forces driving the complex mechanical interaction between embolic material and current stroke thrombectomy devices by analyzing the histological composition and strength of emboli retrieved from patients and by evaluating the mechanical forces necessary for retrieval of such emboli in a middle cerebral artery (MCA) bifurcation model.METHODSEmbolus analogs (EAs) were generated and embolized under physiological pressure and flow conditions in a glass tube model of the MCA. The forces involved in EA removal using conventional endovascular techniques were described, analyzed, and categorized. Then, 16 embolic specimens were retrieved from 11 stroke patients with large-vessel occlusions, and the tensile strength and response to stress were measured with a quasi-static uniaxial tensile test using a custom-made platform. Embolus compositions were analyzed and quantified by histology.RESULTSUniaxial tension on the EAs led to deformation, elongation, thinning, fracture, and embolization. Uniaxial tensile testing of patients’ emboli revealed similar soft-material behavior, including elongation under tension and differential fracture patterns. At the final fracture of the embolus (or dissociation), the amount of elongation, quantified as strain, ranged from 1.05 to 4.89 (2.41 ± 1.04 [mean ± SD]) and the embolus-generated force, quantified as stress, ranged from 63 to 2396 kPa (569 ± 695 kPa). The ultimate tensile strain of the emboli increased with a higher platelet percentage, and the ultimate tensile stress increased with a higher fibrin percentage and decreased with a higher red blood cell percentage.CONCLUSIONSCurrent thrombectomy devices remove emboli mostly by applying linear tensile forces, under which emboli elongate until dissociation. Embolus resistance to dissociation is determined by embolus strength, which significantly correlates with composition and varies within and among patients and within the same thrombus. The dynamic intravascular weakening of emboli during removal may lead to iatrogenic embolization.

Numerical Study of the Prestressed Connectors and Their Distribution on the Strength of a Single Lap, a Double Lap and Hybrid Joints Subjected to Uniaxial Tensile Test

2013 ◽  
Vol 58 (2) ◽  
pp. 579-585 ◽  
Author(s):  
T. Sadowski ◽  
P. Golewski
Keyword(s):  
Mechanical Response ◽  
Numerical Study ◽  
Lap Joints ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Shear Stresses ◽  
Lap Joint ◽  
Positive Effects ◽  
Single Lap Joint ◽  
Hybrid Joints

Prestressed joints are widely used in construction using connectors in the form of screws, whose task is to strong clamping of joined parts, thereby the internal forces in joint are transferred by surface friction contact of the elements. In the automotive and aerospace industries hybrid joints are more widely applied. Mechanical connectors are added to the adhesive joint in form of rivets, screws or clinch increasing its strength properties. The aim of this study was to determine how the prestressed connectors influence the mechanical response of hybrid, single and double lap joints. The influence of different distribution of the connectors was also investigated. Numerical study was conducted in ABAQUS program. Mechanical connectors were modeled by using fasteners, that allowed for a considerable simplification of the numerical model. In their application, there is no need for an additional submodels for connectors in the form of the rivet or the bolt. Prestressing is activated by direct application of the force to the connector. In the numerical examples the authors assumed that the diameter of the mechanical connectors was equal to 6mm and shear strength was equal 1kN. Adhesive layers were modeled by using cohesive elements for which maximum shear stresses and fracture energy were specified. The layer thickness was assumed to be equal 0.1mm and it was initially removed from the areas where mechanical connectors were placed. Two types of joints were analysed in the study: the single lap joint with lap dimensions 40x40mm as well as the double lap joint with lap dimensions 40x20mm, from which it results that theoretical strength of both connections should be the same. The prestressing of connectors was introduced by the force 1.5kN. For all pure - mechanical joints and for single lap joints positive effects were obtained. For double lap joints additional prestressing did not significantly affect for their strength. The influence of distribution of mechanical connectors was additionally analyzed by consideration of three configurations, where the rows of rivets were located at distances of 5, 10 and 15mm from the lap edge. The maximum increase of the load capacity by 24% was achieved for single lap joint as well as 35.7% for double lap joint. The obtained numerical results indicate the positive effects of additional pressure and allows for practical suggestions how to correct and optimize spacing distance of mechanical connectors in hybrid joints to get better mechanical response.

Mechanical Response of Ti-6Al-4V Alloy on Deformation at Moderate Temperatures

2013 ◽  
Vol 549 ◽  
pp. 311-316 ◽  
Author(s):  
Marion Merklein ◽  
Hinnerk Hagenah ◽  
Markus Kaupper ◽  
Adam Schaub
Keyword(s):  
Titanium Alloy ◽  
Elevated Temperatures ◽  
Mechanical Response ◽  
Material Behavior ◽  
High Yield ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Forming Process ◽  
Element Analysis ◽  
Specific Strength

Due to beneficial characteristics such as high specific strength, corrosion resistance and biocompatibility Ti-6Al-4V alloy has become the most important industrially produced titanium alloy during the last decades. Commonly used for aerospace technology and medical products, nowadays Ti-6Al-4V covers 50% of the worldwide produced titanium alloy parts. Different deformation operations as forging and casting as well as machining are used to shape titanium alloy components. For sheet metals, cost and time of fabrication can be reduced significantly via the near net shape technology sheet metal forming. Materials such as the α + β alloy Ti-6Al-4V with high yield stress and comparatively low elastic modules need to be formed at elevated temperatures to increase their formability. Numerical simulations are applied to calculate the forming behavior during the process and conclude the characteristics of the shaped part. Therefore in this paper the mechanical behavior of this titanium alloy is investigated by uniaxial tensile test within elevated temperatures ranging from 250 to 500 °C. Finally, the experimental results are adapted to models which predict the flow response in order to describe material behavior in finite element analysis of the forming process.

scholarly journals Reversible platelet aggregation in the presence of calcium ions: mechanisms and potential value

2019 ◽  
Vol 18 (3) ◽  
pp. 120-129
Author(s):  
A. A. Filkova ◽  
M. A. Panteleev ◽  
A. N. Sveshnikova
Keyword(s):  
Platelet Aggregation ◽  
Calcium Ions ◽  
Light Transmission ◽  
Platelet Rich Plasma ◽  
Reversible Aggregation ◽  
Blood Clots ◽  
Platelet Concentration ◽  
Irreversible Aggregation ◽  
Platelet Aggregates

Disorders in the functions of platelets – blood cells responsible for the blood clots formation and prevention – are observed as independent diseases, as a complication of cancer and hematological diseases or as a result of a therapy. Nowadays, a test of platelet aggregation by aggregometry is the only diagnostic method for assessing the platelets functions. There are several varieties of aggregometry, which differ both in the method of recording the formation of platelet aggregates and in the method of preparing platelets for the experiment. In most laboratories, it is customary to conduct aggregometry in platelet-rich plasma in the presence of citrate ions. In this case, the concentration of calcium ions in plasma decreases, it prevents the thrombin formation and the plasma coagulation. On the other hand, it has long been known that platelet aggregation in response to ADP in the presence of calcium ions (in blood plasma collected in heparin or hirudin tubes, also blocking plasma clotting) is reversible: after 1-5 minutes after the addition of the activator, the disaggregation begins until the light transmission of the solution (platelet concentration) returns to its original level. This phenomenon is called "reversible” platelet aggregation. Reversible aggregation (“disaggregation”) is sometimes observed in aggregometry of citrate plasma, especially in pediatric patients. However, it is usually not considered normal and is considered a sign of platelet dysfunction. This review considers the known mechanisms of disaggregation in the presence or absence of calcium ions in the medium. The role of secondary activation of platelets as a potential cause of irreversible aggregation is discussed, as well as possible versions for explaining the results of aggregometry, when reversible platelet aggregation is observed.

Investigating Ductile Failure at the Microscale in Engineering Steels: A Micromechanical Finite Element Model

2012 ◽  
Author(s):  
Dong-Feng Li ◽  
Noel P. O’Dowd
Keyword(s):  
Mechanical Response ◽  
Micromechanical Model ◽  
Equivalent Plastic Strain ◽  
Ductile Failure ◽  
Austenitic Stainless Steels ◽  
Element Model ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Martensitic Steels ◽  
Crystallographic Slip

In this study, we present a microstructure-based micromechanical model to quantify failure mechanisms in engineering steels. Crystal plasticity at the microscale, governed by crystallographic slip, is explicitly taken into account in the frame-work of continuum mechanics. Furthermore, it is assumed that material damage at the microscale is controlled by the accumulated equivalent plastic strain, such that failure occurs once this strain exceeds a threshold. Both single- and poly-crystalline materials containing sufficient numbers of grains are investigated under a representative macroscopic loading. The calibration of the present model relies on uniaxial tensile test data. Both austenitic stainless steels (such as 316H) and martensitic steels (such as P91) are examined to illustrate the application of the method. The micromechanical modelling provides insights into understanding of the mechanical response at the microscale in engineering steels.

Ticlopidine Facilitates the Deaggregation of Human Platelets Aggregated by Thrombin

1994 ◽  
Vol 71 (01) ◽  
pp. 091-094 ◽  
Author(s):  
M Cattaneo ◽  
B Akkawat ◽  
R L Kinlough-Rathbone ◽  
M A Packham ◽  
C Cimminiello ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Platelet Aggregation ◽  
Prostaglandin E1 ◽  
Human Platelet ◽  
Adenosine Diphosphate ◽  
Human Platelets ◽  
Before And After ◽  
Normal Human ◽  
Platelet Aggregates ◽  
Induced Aggregation

SummaryNormal human platelets aggregated by thrombin undergo the release reaction and are not readily deaggregated by the combination of inhibitors hirudin, prostaglandin E1 (PGE1) and chymotrypsin. Released adenosine diphosphate (ADP) plays an important role in the stabilization of thrombin-induced human platelet aggregates. Since ticlopidine inhibits the platelet responses to ADP, we studied thrombin-induced aggregation and deaggregation of 14C-serotonin-labeled platelets from 12 patients with cardiovascular disease before and 7 days after the oral administration of ticlopidine, 250 mg b.i.d. Before and after ticlopidine, platelets stimulated with 1 U/ml thrombin aggregated, released about 80–90% 14C-serotinin and did not deaggregate spontaneously within 5 min from stimulation. Before ticlopidine, hirudin (5× the activity of thrombin) and PGE1 (10 μmol/1) plus chymotrypsin (10 U/ml) or plasmin (0.06 U/ml), added at the peak of platelet aggregation, caused slight or no platelet deaggregation. After ticlopidine, the extent of platelet deaggregation caused by the same inhibitors was significantly greater than before ticlopidine. The addition of ADP (10 μmol/1) to platelet suspensions 5 s after thrombin did not prevent the deaggregation of ticlopidine-treated platelets. Thus, ticlopidine facilitates the deaggregation of thrombin-induced human platelet aggregates, most probably because it inhibits the effects of ADP on platelets.

Accumulation of Macromolecular Particles in the Reticuloendothelial System (RES) Mediated by Platelet Aggregation and Disaggregation

1969 ◽  
Vol 22 (03) ◽  
pp. 496-507 ◽  
Author(s):  
W.G van Aken ◽  
J Vreeken
Keyword(s):  
Platelet Aggregation ◽  
Degradation Products ◽  
Reticuloendothelial System ◽  
Caproic Acid ◽  
Carbon Particles ◽  
Carbon Clearance ◽  
Aggregation And Disaggregation ◽  
Platelet Aggregates

SummaryCarbon particles cause platelet aggregation in vitro and in vivo. Prior studies established that substances which modify thrombocyte aggregation also influence the rate at which carbon is cleared from the blood.This study was performed in order to elucidate the mechanism by which the carbon-platelet aggregates specifically accumulate in the RES.Activation of fibrinolysis by urokinase or streptokinase reduced the carbon clearance rate, probably due to generated fibrinogen degradation products (FDP). Isolated FDP decreased the carbon clearance and caused disaggregation of platelets and particles in vitro. Inhibition of fibrinolysis by epsilon-amino-caproic acid (EACA), initially accelerated the disappearance of carbon and caused particle accumulation outside the RES, predominantly in the lungs. It is supposed that platelet aggregation and locally activated fibrinolysis act together in the clearance of particles. In the normal situation the RES with its well known low fibrinolytic activity, becomes the receptor of the particles.

Effects of Bupranolol, a New β-Blocker, on Platelet Functions of Rabbit and Human in Vitro

1976 ◽  
Vol 36 (02) ◽  
pp. 376-387 ◽  
Author(s):  
Teruhiko Umetsu ◽  
Kazuko Sanai ◽  
Tadakatsu Kato
Keyword(s):  
Platelet Aggregation ◽  
Platelet Adhesion ◽  
Human Platelets ◽  
Rabbit Platelet ◽  
Rabbit Platelets ◽  
Β Blocker ◽  
Platelet Aggregates ◽  
Induced Aggregation ◽  
Platelet Functions

SummaryThe effects of bupranolol, a new β-blocker, on platelet functions were investigated in vitro in rabbits and humans as compared with propranolol, a well-known β-blocker. At first, the effect of adrenaline on ADP-induced rabbit platelet aggregation was studied because adrenaline alone induces little or no aggregation of rabbit platelets. Enhancement of ADP-induced rabbit platelet aggregation by adrenaline was confirmed, as previously reported by Sinakos and Caen (1967). In addition the degree of the enhancement was proved to be markedly affected by the concentration of ADP and to increase with decreasing concentration of ADP, although the maximum aggregation (percent) was decreased.Bupranolol and propranolol inhibited the (adrenaline-ADP-)induced aggregation of rabbit platelets, bupranolol being approximately 2.4–3.2 times as effective as propranolol. Bupranolol stimulated the disaggregation of platelet aggregates induced by a combination of adrenaline and ADP, but propranolol did not. Platelet adhesion in rabbit was also inhibited by the β-blockers and bupranolol was more active than propranolol. With human platelets, aggregation induced by adrenaline was inhibited by bupranolol about 2.8–3.3 times as effectively as propranolol.From these findings. We would suggest that bupranolol might be useful for prevention or treatment of thrombosis.

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