scholarly journals Demographic Analysis of Mutations in Indian SARS-CoV-2 Isolates

2021 ◽  
Author(s):  
Nita Parekh ◽  
Kushagra Agarwal
Keyword(s):  
Genetic Analysis ◽  
Tamil Nadu ◽  
Principal Component ◽  
Contact Tracing ◽  
The Past ◽  
First Case ◽  
Indian Isolates ◽  
One Year ◽  
Demographic Distribution ◽  
Mutation Spectra

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) originally emerged from Hubei province, Wuhan, China, and has caused a worldwide pandemic with millions affected since December 2019. Since the first case of SARS-CoV-2 reported on 27th Jan 2020 in India, over 11 million people have been affected by the virus in the country in the past one year. To understand its demographic distribution, in this study we carried out the early distribution of clades and subclades state-wise based on the analysis of shared mutations in Indian SARS-CoV-2 isolates collected during the period 27th Jan – 27th May 2020. A total of 1279 mutations have been identified in 685 Indian isolates during this period. Principal component analysis on the mutational profile of Indian isolates revealed clusters corresponding to clades in Nextstrain and some state-specific clusters. Phylogenetic analysis of these isolates indicates multiple independent sources of introduction of the virus in the country. It is observed that clade 20A defining mutations C241T (ORF1ab: 5′ UTR), C3037T (ORF1ab: F924F), C14408T (ORF1ab: P4715L), and A23403G (S: D614G) are the predominant mutation set in Indian isolates during this period. Higher number of coronavirus cases were observed in certain states, viz., Delhi, Tamil Nadu, and Telangana. Genetic analysis of isolates from these states revealed a cluster with shared mutations, C6312A (ORF1ab: T2016K), C13730T (ORF1ab: A4489V), C23929T, and C28311T (N: P13L). Contact tracing led the spread of this cluster to a super spreader event, the Tablighi Jamaat religious congregation, and the efficacy of lockdown in containing it. Analysis of region-specific shared mutations carried out to understand the large number of deaths in Gujarat and Maharashtra identified shared mutations defining subclade, I/GJ-20A (C18877T, C22444T, G25563T (ORF3a: H57Q), C26735T, C28854T (N: S194L), C2836T) in Gujarat and two sets of co-occurring mutations C313T, C5700A (ORF1ab: A1812D) and A29827T, G29830T in Maharashtra. From the genetic analysis of mutation spectra of Indian isolates, the insights gained in its transmission, geographic distribution, containment, and impact are discussed.

scholarly journals Pediatric Extraspinal Sacrococcygeal Ependymoma: Report of Two Cases and Literature Review

Diagnostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1680
Author(s):  
Francesco Fabozzi ◽  
Silvia Ceccanti ◽  
Antonella Cacchione ◽  
Giovanna Stefania Colafati ◽  
Andrea Carai ◽  
...  
Keyword(s):  
Literature Review ◽  
Case Reports ◽  
Case Series ◽  
Surgical Excision ◽  
Central Canal ◽  
Ependymal Cells ◽  
The Past ◽  
First Case ◽  
One Year

Primary central nervous system (CNS) tumors represent the most common solid tumors in childhood. Ependymomas arise from ependymal cells lining the wall of ventricles or central canal of spinal cord and their occurrence outside the CNS is extremely rare, published in the literature as case reports or small case series. We present two cases of extra-CNS myxopapillary ependymomas treated at our institution in the past three years; both cases originate in the sacrococcygeal region and were initially misdiagnosed as epidermoid cyst and germ cell tumor, respectively. The first case, which arose in a 9-year-old girl, was treated with a surgical excision in two stages, due to the non-radical manner of the first operation; no recurrence was observed after two years of follow-up. The other case was a 12-year-old boy who was treated with a complete resection and showed no evidence of recurrence at one-year follow-up. In this paper, we report our experience in treating an extremely rare disease that lacks a standardized approach to diagnosis, treatment and follow-up; in addition, we perform a literature review of the past 35 years.

Use of E-Resources by Social Scientists in Tamil Nadu, India

2019 ◽  
pp. 146-173
Author(s):  
C. Muthurasu ◽  
Sri. P. Kannan
Keyword(s):  
Full Text ◽  
Tamil Nadu ◽  
Computer Access ◽  
University Libraries ◽  
Social Scientists ◽  
Electronic Journals ◽  
The Past ◽  
Optical Media ◽  
Electronic Product ◽  
One Year

E-resources is defined as a resource which requires computer access or any electronic product that delivers a collection of data, be it text referring to full text bases, electronic journals, image collections, other multimedia products, as a commercially available title that has been published with an aim to being marketed. Over the past few years, a number of techniques about related standards have been developed which allow documents to be created and distributed in electronic form. The e-resource on magnetic and optical media has a vast impact on the collections of university libraries. The study shows that majority of the respondents—82.59% of respondents having 1-2 years' experience, followed by less than one year (11.08%), 3-5 years (3.48%), and only 2.85% of respondents stated more than five years—have experience of using e-resources.

scholarly journals A case report on meningococcal septicaemia in Kalabazaar, Thimphu, November 2016

2016 ◽  
Vol 3 (1) ◽  
pp. 15
Author(s):  
Tsheten . ◽  
Sonam Wangchuk ◽  
Mimi Lhamu Mynak ◽  
Tenzin Lhaden
Keyword(s):  
Contact Tracing ◽  
Post Exposure Prophylaxis ◽  
The Past ◽  
Meningococcal Septicaemia ◽  
History Of ◽  
One Year ◽  
The One ◽  
Exposure Prophylaxis ◽  
Close Contacts

<p>We report here a case study of meningococcal septicaemia caused by <em>Neisseria meningitidis</em> in Kalabazaar, Thimphu on November 2016. The one-year old patient had no history of contact with any infected individuals in the past two weeks before his onset, neither did he travel anywhere besides his one-storey traditional residence. The patient was hospitalized and given antibiotic treatment of ceftriaxone at an early course of the disease. The patient did not develop any complications during the time of hospitalization and had recovered well. Close contacts with the case were identified and given post-exposure prophylaxis with 500 mg ciprofloxacin. No new cases were encountered during the course of contact tracing. </p>

Osseointegration interface of calcified bone and endosseous implants

1992 ◽  
Vol 50 (2) ◽  
pp. 1096-1097
Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley
Keyword(s):  
Structure And Function ◽  
Titanium Implants ◽  
En Bloc ◽  
Endosseous Implants ◽  
Ultrastructural Studies ◽  
The Past ◽  
Cacodylate Buffer ◽  
One Year ◽  
And Function

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.

Genetic Analysis of Chinese Cucumber Collections in the U.S. National Germplasm Collection

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 534e-534 ◽  
Author(s):  
J. Staub ◽  
Felix Sequen ◽  
Tom Horejsi ◽  
Jin Feng Chen
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Genetic Variation ◽  
Genetic Analysis ◽  
Allelic Variation ◽  
Germplasm Collection ◽  
Principal Component ◽  
Isozyme Loci ◽  
National Plant Germplasm System ◽  
The U.S

Genetic variation in cucumber accessions from China was assessed by examining variation at 21 polymorphic isozyme loci. Principal component analysis of allelic variation allowed for the depiction of two distinct groupings of Chinese accessions collected in 1994 and 1996 (67 accessions). Six isozyme loci (Gpi, Gr, Mdh-2, Mpi-2, Pep-gl, and Pep-la) were important in elucidating these major groups. These groupings were different from a single grouping of Chinese 146 accessions acquired before 1994. Allelic variation in Chinese accessions allowed for comparisons with other accessions in the U.S. National Plant Germplasm System (U.S. NPGS) collection grouped by continent and sub-continent. When Chinese accessions taken collectively were compared with an array of 853 C. sativus U.S. NPGS accessions examined previously, relationships differed between accessions grouped by country or subcontinent. Data indicate that acquisition of additional Chinese and Indian cucumber accessions would be strategically important for increasing genetic diversity in the U.S. NPGS cucumber collection.

Sampling, Preservation and Counting of Samples II: Zooplankton

2017 ◽  
Author(s):  
Peter H. Wiebe ◽  
Ann Bucklin ◽  
Mark Benfield
Keyword(s):  
Genetic Analysis ◽  
High Speed ◽  
Morphological Analysis ◽  
Taxonomic Composition ◽  
Sampling Techniques ◽  
Sample Analysis ◽  
Sample Preservation ◽  
The Past ◽  
Plankton Collection

This chapter reviews traditional and new zooplankton sampling techniques, sample preservation, and sample analysis, and provides the sources where in-depth discussion of these topics is addressed. The net systems that have been developed over the past 100+ years, many of which are still in use today, can be categorized into eight groups: non-opening/closing nets, simple opening/closing nets, high-speed samplers, neuston samplers, planktobenthos plankton nets, closing cod-end samplers, multiple net systems, and moored plankton collection systems. Methods of sample preservation include preservation for sample enumeration and taxonomic morphological analysis, and preservation of samples for genetic analysis. Methods of analysis of zooplankton samples include determination of biomass, taxonomic composition, and size by traditional methods; and genetic analysis of zooplankton samples.

scholarly journals Hepatitis and tuberculosis testing are much less common than HIV testing among adults in Kisumu, Kenya: results from a cross-sectional assessment

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Joshua Tunnage ◽  
Adam Yates ◽  
Chiaka Nwoga ◽  
Valentine Sing’oei ◽  
John Owuoth ◽  
...  
Keyword(s):  
Secondary Education ◽  
Viral Hepatitis ◽  
Hiv Testing ◽  
Spatial Location ◽  
Tuberculosis Screening ◽  
Cross Sectional ◽  
The Past ◽  
Prior Testing ◽  
Testing Uptake ◽  
One Year

Abstract Background Kenya has a high burden of HIV, viral hepatitis, and tuberculosis. Screening is necessary for early diagnosis and treatment, which reduces morbidity and mortality across all three illnesses. We evaluated testing uptake for HIV, viral hepatitis, and tuberculosis in Kisumu, Kenya. Methods Cross-sectional data from adults aged 18–35 years who enrolled in a prospective HIV incidence cohort study from February 2017 to May 2018 were analyzed. A questionnaire was administered to each participant at screening for study eligibility to collect behavioral characteristics and to assess prior testing practices. Among participants without a history of previously-diagnosed HIV, multivariable robust Poisson regression was used to estimate prevalence ratios (PRs) and 95% confidence intervals (CIs) for factors potentially associated with HIV testing in the 12 months prior to enrollment. A hierarchical model was used to test for differential access to testing due to spatial location. Results Of 671 participants, 52 (7.7%) were living with HIV, 308 (45.9%) were female, and the median age was 24 (interquartile range 21–28) years. Among 651 (97.0%) who had ever been tested for HIV, 400 (61.2%) reported HIV testing in the past 6 months, 129 (19.7%) in the past 6–12 months, and 125 (19.1%) more than one year prior to enrollment. Any prior testing for viral hepatitis was reported by 8 (1.2%) participants and for tuberculosis by 51 (7.6%). In unadjusted models, HIV testing in the past year was more common among females (PR 1.08 [95% CI 1.01, 1.17]) and participants with secondary education or higher (PR 1.10 [95% CI 1.02, 1.19]). In the multivariable model, only secondary education or higher was associated with recent HIV testing (adjusted PR 1.10 [95% CI 1.02, 1.20]). Hierarchical models showed no geographic differences in HIV testing across Kisumu subcounties. Conclusions Prior HIV testing was common among study participants and most had been tested within the past year but testing for tuberculosis and viral hepatitis was far less common. HIV testing gaps exist for males and those with lower levels of education. HIV testing infrastructure could be leveraged to increase access to testing for other endemic infectious diseases.

Language as an Identity: Hindi and Provincial Languages in Colonial India

2021 ◽  
Vol 7 (2) ◽  
pp. 314-337
Author(s):  
Amit Ranjan
Keyword(s):  
Tamil Nadu ◽  
Linguistic Identity ◽  
Colonial India ◽  
Nationalist Party ◽  
The Past ◽  
The People ◽  
Per Se ◽  
Hindi Language ◽  
Inherent Part ◽  
Union Government

Language is an inherent part of an individual’s identity. Any attempt to subjugate that identity is vehemently resisted by the people. In India, Hindi is not only seen as a language per se but also linked with North Indian Hindus. In the past, the introduction and imposition of Hindi in non-Hindi-speaking states, mainly Tamil Nadu, had faced strong opposition. Since the National Democratic Alliance (NDA) led by the Hindu Nationalist Party—Bhartiya Janata Party (BJP)—elected to power in May 2014, the union government has taken measures to what it calls promoting the use of the Hindi language in India. These measures have been strongly resisted in the non-Hindi-speaking states of the country. This article looks at the debates between Hindi and non-Hindi speakers since the years of the anti-colonial movement in India. It examines the character of the movement to promote Hindi and the resistance against the Hindi movements in India. This article also discusses the demands for language-based states in India. In this paper, the author argues that in the non-Hindi-speaking states, Hindi is mainly looked at as a means to subsume and suppress the native’s identity. To protect their linguistic identity, which is inextricably intertwined with other identities, people in non-Hindi-speaking areas have protested in past and also resist such attempts in present.

scholarly journals Impact of Coronavirus Outbreaks on Science and Society: Insights from Temporal Bibliometry of SARS and COVID-19

Entropy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 626
Author(s):  
Ramya Gupta ◽  
Abhishek Prasad ◽  
Suresh Babu ◽  
Gitanjali Yadav
Keyword(s):  
Published Data ◽  
Science And Society ◽  
Time Dimension ◽  
Scientific Publications ◽  
The Past ◽  
Economic Trends ◽  
Global Events ◽  
One Year ◽  
Personal Devices ◽  
Share Information

A global event such as the COVID-19 crisis presents new, often unexpected responses that are fascinating to investigate from both scientific and social standpoints. Despite several documented similarities, the coronavirus pandemic is clearly distinct from the 1918 flu pandemic in terms of our exponentially increased, almost instantaneous ability to access/share information, offering an unprecedented opportunity to visualise rippling effects of global events across space and time. Personal devices provide “big data” on people’s movement, the environment and economic trends, while access to the unprecedented flurry in scientific publications and media posts provides a measure of the response of the educated world to the crisis. Most bibliometric (co-authorship, co-citation, or bibliographic coupling) analyses ignore the time dimension, but COVID-19 has made it possible to perform a detailed temporal investigation into the pandemic. Here, we report a comprehensive network analysis based on more than 20,000 published documents on viral epidemics, authored by over 75,000 individuals from 140 nations in the past one year of the crisis. Unlike the 1918 flu pandemic, access to published data over the past two decades enabled a comparison of publishing trends between the ongoing COVID-19 pandemic and those of the 2003 SARS epidemic to study changes in thematic foci and societal pressures dictating research over the course of a crisis.

Penile Lymphoepithelioma-Like Carcinoma: A Rare Case With PD-L1 Expression

2021 ◽  
pp. 106689692098834
Author(s):  
Raquel Machado-Neves ◽  
Bernardo Teixeira ◽  
Elsa Fonseca ◽  
Pedro Valente ◽  
Joaquim Lindoro ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Tumor Cells ◽  
Rare Case ◽  
Malignant Tumors ◽  
Squamous Cell Carcinomas ◽  
The Third ◽  
The Past ◽  
First Case ◽  
Lymphoplasmacytic Infiltrate

Most malignant tumors of the penis are squamous cell carcinomas (SCC), being divided in 2 groups, one human papillomavirus (HPV)-related and another non-HPV-related, with lymphoepithelioma-like carcinoma (LELC) being one of the rarest HPV-related SCC. In this article, we report a case of a 50-year-old man who presented testicular swelling and pain for the past 3 months. A penile mass was identified, and the patient was submitted to a total penectomy. The penectomy specimen showed an ulcerated lesion at the glans reaching the cavernous bodies. Microscopic examination showed undifferentiated epithelial cells with syncytial growth pattern mix with a dense lymphoplasmacytic infiltrate, consistent with LELC. The tumor cells expressed p16 and all 3 different clones of PDL1 (22C3, SP263, and SP142). The patient is alive and well with a follow-up of 3 months. To our knowledge, this is the third LELC of the penis reported in literature and the first case reported with PDL1 expression.

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