scholarly journals Drosophila have distinct activity-gated pathways that mediate attraction and aversion to CO2

2017 ◽  
Author(s):  
Floris van Breugel ◽  
Ainul Huda ◽  
Michael H. Dickinson
Keyword(s):  
Laboratory Experiments ◽  
Respiratory Acidosis ◽  
Future Research ◽  
Yeast Fermentation ◽  
Activity Levels ◽  
Behavioral Differences ◽  
Natural Processes ◽  
Broad Signal ◽  
High Concentrations ◽  
Low Activity

Carbon dioxide is a volatile and broad signal of many organic processes, and serves as a convenient cue for insects in search of blood hosts1–6, flowers7, decaying matter8–11, communal nests12, fruit13, and wildfires14. Curiously, although Drosophila melanogaster feed on yeast that produce CO2 and ethanol during fermentation, laboratory experiments suggest that flies actively avoid CO215–25. Here, we resolve this paradox by showing that both flying and walking fruit flies do actually find CO2 attractive, but only when they are in an active state associated with foraging. Aversion at low activity levels may be an adaptation to avoid CO2-seeking-parasites, or succumbing to respiratory acidosis in the presence of high concentrations of CO2 that are occasionally found in nature26,27. In contrast to CO2, flies are attracted to ethanol in all behavioral states, and invest twice as much time searching near ethanol compared to CO2. These behavioral differences reflect the fact that whereas CO2 is a generated by many natural processes, ethanol is a unique signature of yeast fermentation. Using genetic tools, we determined that the evolutionarily ancient ionotropic co-receptor IR25a is required for both CO2 and ethanol attraction, and that the receptors previously identified for CO2 avoidance are not involved. Our study lays the foundation for future research to determine the neural circuits underlying both state- and odorant-dependent decision making in Drosophila.

Brown pigmentation in Serratia marcescens cultures associated with tyrosine metabolism

1989 ◽  
Vol 35 (11) ◽  
pp. 1037-1042 ◽  
Author(s):  
Joaquim Trias ◽  
Miquel Viñas ◽  
Jesus Guinea ◽  
José G. Lorèn
Keyword(s):  
Serratia Marcescens ◽  
Minimal Medium ◽  
Brown Pigment ◽  
Activity Levels ◽  
Amino Acid Catabolism ◽  
Catabolic Pathway ◽  
Tyrosine Metabolism ◽  
High Concentrations ◽  
Low Activity ◽  
Production Conditions

Serratia marcescens produced a brown pigment when grown in minimal medium in the presence of tyrosine and high concentrations of copper(II) ion. The pigment was not related to the melanin pigments, but was similar to the pigment produced by autooxidation and polymerization of 3,4-dihydroxyphenylacetate, which is synthesized in S. marcescens from tyrosine through the 3,4-dihydroxyphenylacetate catabolic pathway. The enzymes of this pathway were induced under pigment production conditions; however, 3,4-dihydroxyphenylacetate 2,3-dioxygenase remained at low activity levels, permitting the accumulation and excretion of the substrate. Mutants unable to use tyrosine as a sole carbon and energy source were able to produce brown pigments only if the step blocked by the mutation was after the synthesis of 3,4-dihydroxyphenylacetate. The ability to produce brown pigments was common to all the S. marcescens strains tested.Key words: brown pigment, 3,4-dihydroxyphenylacetate 2,3-dioxygenase, Serratia marcescens, tyrosine, aromatic amino acid catabolism.

scholarly journals Peripheral edema: A common and persistent health problem for older Americans

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260742
Author(s):  
Soroush Besharat ◽  
Hanna Grol-Prokopczyk ◽  
Shan Gao ◽  
Changyong Feng ◽  
Frank Akwaa ◽  
...  
Keyword(s):  
Minority Groups ◽  
Cost Effective ◽  
Limited Range ◽  
Future Research ◽  
Activity Levels ◽  
Mobility Limitations ◽  
Older Americans ◽  
Peripheral Edema ◽  
Limited Range Of Motion ◽  
Low Activity

Peripheral edema (i.e., lower limb swelling) can cause pain, weakness, and limited range of motion. However, few studies have examined its prevalence in the U.S. or its association with demographics, comorbidities, activity, or mobility. This study used data from the Health and Retirement Study, a nationally representative longitudinal survey of U.S. adults (age 51+/ N = 19,988 for 2016), to evaluate time trends and correlates of peripheral edema using weighted descriptive statistics and logistic regressions, respectively. Peripheral edema was assessed with the question “Have you had… // Persistent swelling in your feet or ankles?” The weighted prevalence of edema among older U.S. adults was 19% to 20% between 2000 and 2016. Peripheral edema was associated with older age, female sex, non-white race, low wealth, obesity, diabetes, hypertension, pain, low activity levels, and mobility limitations (odds ratios ranging from 1.2–5.6; p-values ≤0.001). This study provides the first estimates of national prevalence and correlates of peripheral edema among older Americans. Peripheral edema is common and strongly associated with comorbidities, pain, low activity levels, and mobility limitations, and disproportionately affects poorer and minority groups. Peripheral edema should be a focus of future research in order to develop novel and cost-effective interventions.

Potential Contribution of Nutrients and Polycyclic Aromatic Hydrocarbons from the Creeks of Cootes Paradise Marsh

1996 ◽  
Vol 31 (3) ◽  
pp. 485-504 ◽  
Author(s):  
Patricia Chow-Fraser ◽  
Barb Crosbie ◽  
Douglas Bryant ◽  
Brian McCarry
Keyword(s):  
Aromatic Hydrocarbons ◽  
Laboratory Experiments ◽  
Dry Weight ◽  
Common Source ◽  
Potential Contribution ◽  
Nitrogen And Phosphorus ◽  
Engine Combustion ◽  
Polycyclic Aromatic ◽  
High Concentrations ◽  
Derivatives Of

Abstract During the summer of 1994, we compared the physical and nutrient characteristics of the three main tributaries of Cootes Paradise: Spencer, Chedoke and Borer’s creeks. On all sampling occasions, concentrations of CHL α and nutrients were always lowest in Borer’s Creek and highest in Chedoke Creek. There were generally 10-fold higher CHL α concentrations and 2 to 10 times higher levels of nitrogen and phosphorus in Chedoke Creek compared with Spencer Creek. Despite this, the light environment did not differ significantly between Spencer and Chedoke creeks because the low algal biomass in Spencer Creek was balanced by a relatively high loading of inorganic sediments from the watershed. Laboratory experiments indicated that sediments from Chedoke Creek released up to 10 µg/g of soluble phosphorus per gram (dry weight) of sediment, compared with only 2 µg/g from Spencer Creek. By contrast, sediment samples from Spencer Creek contained levels of polycyclic aromatic hydrocarbon that were as high as or higher than those from Chedoke Creek, and much higher than those found in Borer’s Creek. The distribution of normalized PAH concentrations suggests a common source of PAHs in all three tributaries, most likely automobile exhaust, since there were high concentrations of fluoranthene and pyrene, both of which are derivatives of engine combustion.

379 Do Depressive Symptoms Mediate the Association Between Insomnia and Physical Activity?

SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A150-A151
Author(s):  
Jamie Walker ◽  
Rebecca Campbell ◽  
Ivan Vargas
Keyword(s):  
Physical Activity ◽  
Depressive Symptoms ◽  
Online Survey ◽  
Longitudinal Design ◽  
Energy Levels ◽  
Depression Scale ◽  
Severity Index ◽  
Future Research ◽  
Activity Levels ◽  
Insomnia Symptoms

Abstract Introduction Insomnia and depression are highly comorbid and have been shown to be independently associated with lower levels of physical activity. It is not clear, however, if being less physically active is a risk factor for or consequence of depression and insomnia. The factors that explain the associations between insomnia, depression, and physical activity are likely complex and overlapping. For example, insomnia may predict inactivity by impacting one’s energy levels, leaving them too tired to exercise. Insomnia may also interfere with one’s motivation to exercise due to low mood, as insomnia is associated with the development of depressive symptoms. The purpose of the present study was to explore whether depression mediated the link between insomnia and low levels of physical activity. Methods A national online survey was conducted from April-June 2020. Participants completed surveys to assess demographics, mood, sleep, and physical activity. Depressive symptoms were estimated with the Center for Epidemiologic Studies Depression Scale (CES-D). Insomnia symptoms were estimated with the Insomnia Severity Index (ISI). Physical activity levels were estimated with the International Physical Activity Questionnaire (IPAQ). Analyses were conducted using multiple linear regression, with separate models for depression, insomnia, and the combination of the two, on levels of physical activity. Results 3,952 adults (Mage = 46.9 years) completed the survey. According to the unadjusted models, greater insomnia symptoms were associated with greater depressive symptoms (b = 0.4523, SE = 0.019593, p < .001), and lower levels of physical activity (b = -38.741, SE = 18.236, p = 0.0337). The relationship between insomnia and physical activity was no longer significant, however, when controlling for depression (b = -6.140, SE = 19.274, p = 0.75). According to the mediation analyses, there was an indirect effect of insomnia on physical activity that was explained by differences in depressive symptoms (Sobel Test = -4.895, SE = 6.518, p < .001). Conclusion Our findings support previous research indicating associations between symptoms of insomnia and depression and physical activity. Future research should examine if these same results hold using a longitudinal design. Support (if any) Vargas: K23HL141581

Mediators of Estimates of Brief Time Intervals in Elderly Domiciled Males

1986 ◽  
Vol 21 (3) ◽  
pp. 211-225 ◽  
Author(s):  
David Licht ◽  
John B. Morganti ◽  
Milton F. Nehrke ◽  
Gary Heiman
Keyword(s):  
Production Method ◽  
Passage Time ◽  
Future Research ◽  
Value Of Time ◽  
Activity Levels ◽  
Time Intervals ◽  
Time To Death ◽  
Institutional Settings ◽  
Time Estimates

Conflicting interpretations questioning whether brief intervals of real time are perceived as passing more or less rapidly with increasing age have been reported. Reasons for these inconsistent results lie in semantic confusions, in variations in methodology, and in the effects of other mediating variables. The present study examined relationships between age, value of time over both the short and the long term, perceived time to death, self-perceived activity levels, and estimates of brief time intervals in a sample of older institutionalized males. The production method was used to obtain the estimates of time passage. Time intervals were increasingly underestimated with advancing age, indicating that time units are shorter with increasing age. While a number of other variables were interrelated, the only other factor consistently related to time estimates was the short-term value of time. Implications for future research and for interventions in institutional settings are discussed.

scholarly journals Assessment of Dysfunctional Movements and Asymmetries in Children and Adolescents Using the Functional Movement Screen—A Narrative Review

2021 ◽  
Vol 18 (23) ◽  
pp. 12501
Author(s):  
Pat R. Vehrs ◽  
Martina Uvacsek ◽  
Aaron W. Johnson
Keyword(s):  
Movement Patterns ◽  
Normal Weight ◽  
Future Research ◽  
Physical Educators ◽  
Functional Movement Screen ◽  
Activity Levels ◽  
Obese Children ◽  
Functional Movement ◽  
Test Items ◽  
The Individual

The Functional Movement Screen (FMS) is a screening tool that identifies dysfunctional movements in seven test items requiring an interplay of cognitive, perceptual, proprioceptive, and motor functions that involve muscular strength/endurance, flexibility, mobility, coordination, and balance. The results of the FMS include an overall composite score, scores on the individual test items, and identification of compensatory movement patterns and left-right asymmetries on 5 bilateral test items. Although there is a plethora of literature on the use of the FMS in adults, there is a growing body of evidence indicating its use in children. The available research in children involves school children and young athletes in at least 20 different sports in over 20 countries and comparisons between pre- and post-pubescent children, and normal weight, overweight, and obese children. Studies that include measures of adiposity and physical activity levels, or report prevalence of asymmetries and dysfunctional movement patterns are not well represented in the children’s literature. The purpose of this paper is to synthesize the currently available literature in children and suggest potential uses of the FMS by coaches, physical educators, and other health/fitness professionals, appropriate interpretation of results, and future research in children.

Optimisation of liquid scintillation counting conditions to determine low activity levels of tritium and radiostrontium in aqueous solution

The Analyst ◽  
1990 ◽  
Vol 115 (8) ◽  
pp. 1097 ◽  
Author(s):  
Gemma Rauret ◽  
Joan S. Mestres ◽  
Merc� Ribera ◽  
Pilar Rajadel
Keyword(s):  
Aqueous Solution ◽  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Scintillation Counting ◽  
Activity Levels ◽  
Low Activity

Judgment Based Marketing Decision Models: Problems and Possible Solutions

1981 ◽  
Vol 45 (4) ◽  
pp. 13-23 ◽  
Author(s):  
Dipankar Chakravarti ◽  
Andrew Mitchell ◽  
Richard Staelin
Keyword(s):  
Decision Making ◽  
Comparative Analysis ◽  
Laboratory Experiments ◽  
Field Studies ◽  
Decision Models ◽  
Future Research ◽  
Marketing Decision ◽  
Recent Laboratory

This paper presents a comparative analysis of the findings of two field studies and three recent laboratory experiments that assessed the efficacy of judgment based models in aiding marketing decision making. This analysis indicates factors that may affect the effectiveness of these models. The implications of the findings for users of judgment based marketing decision models as well as model builders are discussed, and suggestions are made for future research to improve the models’ effectiveness.

scholarly journals High-Efficiency Production of Radiopharmaceuticals via Droplet Radiochemistry: A Review of Recent Progress

2020 ◽  
Vol 19 ◽  
pp. 153601212097309
Author(s):  
Jia Wang ◽  
R. Michael van Dam
Keyword(s):  
High Efficiency ◽  
State Of The Art ◽  
System Size ◽  
Scaling Up ◽  
Activity Levels ◽  
Synthesis Time ◽  
Molar Activity ◽  
Reagent Consumption ◽  
Prosthetic Groups ◽  
Low Activity

New platforms are enabling radiochemistry to be carried out in tiny, microliter-scale volumes, and this capability has enormous benefits for the production of radiopharmaceuticals. These droplet-based technologies can achieve comparable or better yields compared to conventional methods, but with vastly reduced reagent consumption, shorter synthesis time, higher molar activity (even for low activity batches), faster purification, and ultra-compact system size. We review here the state of the art of this emerging direction, summarize the radiotracers and prosthetic groups that have been synthesized in droplet format, describe recent achievements in scaling up activity levels, and discuss advantages and limitations and the future outlook of these innovative devices.

scholarly journals Ambulatory Physical Activity Performance in Youth With Cerebral Palsy and Youth Who Are Developing Typically

2007 ◽  
Vol 87 (3) ◽  
pp. 248-257 ◽  
Author(s):  
Kristie F Bjornson ◽  
Basia Belza ◽  
Deborah Kartin ◽  
Rebecca Logsdon ◽  
John F McLaughlin
Keyword(s):  
Cerebral Palsy ◽  
Activity Levels ◽  
Ambulatory Activity ◽  
Cross Sectional ◽  
Walking Activity ◽  
Activity Performance ◽  
Cross Sectional Design ◽  
Low Activity ◽  
Gmfcs Level ◽  
Daily Total

Background and Purpose Assessment of walking activity in youth with cerebral palsy (CP) has traditionally been “capacity-based.” The purpose of this study was to describe the day-to-day ambulatory activity “performance” of youth with CP compared with youth who were developing typically. Subjects Eighty-one youth with CP, aged 10 to 13 years, who were categorized as being in Gross Motor Function Classification System (GMFCS) levels I to III and 30 age-matched youth who were developing typically were recruited. Methods Using a cross-sectional design, participants wore the StepWatch monitor for 7 days while documenting average daily total step counts, percentage of time they were active, ratio of medium to low activity levels, and percentage of time at high activity levels. Results The youth with CP demonstrated significantly lower levels of all outcomes than the comparison group. Discussion and Conclusion Daily walking activity and variability decreased as functional walking level (GMFCS level) decreased. Ambulatory activity performance within the context of the daily life for youth with CP appears valid and feasible as an outcome for mobility interventions in CP.

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