scholarly journals Unleashing floret fertility by a mutated homeobox gene improved grain yield during wheat evolution under domestication

2018 ◽  
Author(s):  
Shun Sakuma ◽  
Guy Golan ◽  
Zifeng Guo ◽  
Taiichi Ogawa ◽  
Akemi Tagiri ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Duplication ◽  
Grain Yield ◽  
Genetic Basis ◽  
Leucine Zipper ◽  
Yield Potential ◽  
Grain Number ◽  
Wheat Evolution ◽  
Grain Number Per Spike ◽  
Cereal Grain

AbstractFloret fertility is a key trait to determine the number of grains per inflorescence in cereals. During wheat (Triticum sp.) evolution, floret fertility has been increased and current bread wheat (T. aestivum L.) produces three to five grains per spikelet; however, little is known about the genetic basis controlling floret fertility. Here we identify the quantitative trait locus Grain Number Increase 1 (GNI1), encoding a homeodomain leucine zipper class I (HD-Zip I) transcription factor. GNI1 evolved in the Triticeae through gene duplication and functionalization. GNI1 was predominantly expressed in the most apical floret primordia and parts of the rachilla, suggesting that GNI1 inhibits rachilla growth and development. GNI1 expression decreased during wheat evolution, and as a consequence, more fertile florets and grains per spikelet are being produced. Genetic analysis revealed that the reduced-function allele of GNI1-A contributes to increase the number of fertile florets per spikelet. The knockdown of GNI1 in transgenic hexaploid wheat improved fertile floret and grain number. Furthermore, wheat plants carrying the impaired allele increased grain yield under field conditions. Our findings illuminate that gene duplication and functionalization generated evolutionary novelty for floret fertility (i.e. reducing floral numbers) while the mutations towards increased grain production were under selection during wheat evolution under domestication. Significance StatementGrain number is a fundamental trait for cereal grain yield; but its underlying genetic basis is mainly unknown in wheat. Here we show for the first time a direct link between increased floret fertility, higher grain number per spike and higher plot-yields of wheat in the field. We have identified GNI1 gene encoding an HD-Zip I transcription factor responsible for increased floret fertility. The wild type allele imposes an inhibitory role specifically during rachilla development, indicating that expression of this protein actively shuts-down grain yield potential; whereas, the reduced-function allele enables more florets and grains to be produced. GNI1 evolved through gene duplication in Triticeae and its mutations were under parallel human selection during wheat and barley evolution under domestication.

scholarly journals Unleashing floret fertility in wheat through the mutation of a homeobox gene

2019 ◽  
Vol 116 (11) ◽  
pp. 5182-5187 ◽  
Author(s):  
Shun Sakuma ◽  
Guy Golan ◽  
Zifeng Guo ◽  
Taiichi Ogawa ◽  
Akemi Tagiri ◽  
...  
Keyword(s):  
Gene Duplication ◽  
Growth And Development ◽  
Genetic Basis ◽  
Leucine Zipper ◽  
Homeobox Gene ◽  
Grain Production ◽  
Evolutionary Novelty ◽  
Grain Number ◽  
Wheat Evolution ◽  
Number Increase

Floret fertility is a key determinant of the number of grains per inflorescence in cereals. During the evolution of wheat (Triticum sp.), floret fertility has increased, such that current bread wheat (Triticum aestivum) cultivars set three to five grains per spikelet. However, little is known regarding the genetic basis of floret fertility. The locus Grain Number Increase 1 (GNI1) is shown here to be an important contributor to floret fertility. GNI1 evolved in the Triticeae through gene duplication. The gene, which encodes a homeodomain leucine zipper class I (HD-Zip I) transcription factor, was expressed most abundantly in the most apical floret primordia and in parts of the rachilla, suggesting that it acts to inhibit rachilla growth and development. The level of GNI1 expression has decreased over the course of wheat evolution under domestication, leading to the production of spikes bearing more fertile florets and setting more grains per spikelet. Genetic analysis has revealed that the reduced-function allele GNI-A1 contributes to the increased number of fertile florets per spikelet. The RNAi-based knockdown of GNI1 led to an increase in the number of both fertile florets and grains in hexaploid wheat. Mutants carrying an impaired GNI-A1 allele out-yielded WT allele carriers under field conditions. The data show that gene duplication generated evolutionary novelty affecting floret fertility while mutations favoring increased grain production have been under selection during wheat evolution under domestication.

scholarly journals Effect of Canopy Structure on Water Use of Wheat (Triticum Aestivum L.) During Post-Anthesis Stage

2018 ◽  
Vol 1 (1) ◽  
pp. 116-121
Author(s):  
Özgür Tatar ◽  
Uğur Çakaloğullari ◽  
Gülden Deniz Ateş Atasoy ◽  
Deniz Iştipliler
Keyword(s):  
Soil Moisture ◽  
Grain Yield ◽  
Soil Water ◽  
Water Status ◽  
Canopy Cover ◽  
Yield Potential ◽  
Grain Number ◽  
Soil Water Status ◽  
Grain Number Per Spike ◽  
Spike Number

AbstractWheat, being the main crop in Mediterranean type environments, is grown during winter under rainfed conditions and its yield potential is mostly affected by the amount and distribution of rain. This study is conducted at experimental fields of Ege University, Department of Field Crops in Izmir-Bornova which is characterized as Mediterranean type climate conditions during 2011/2012 and 2012/2013 growing season. Totally 9 canopy structures were generated by different row and line distances. Plant height, total dry weight, tiller number, harvest index, spike number, grain number per spike, thousand grain weight and grain yield were determined after harvest time while digital leaf area index and soil moisture contents were monitoring during specific growth periods to evaluate changes in soil water status by different canopy closer. Increasing in sowing density by different row and in-line distance reduced tiller and spike number per plant. On the other hand, grain number per spike and thousand grain weights were significantly affected by in-line distance instead of row distance. Higher grain yield were obtained from 20x1 cm treatments. Canopy cover speed determined using with digital imaging was not remarkable correlated (r=0.06) with soil moisture content during post-anthesis stage of wheat when higher rain amount is recorded in 2012. However, there was a significant negative correlation (r=0.51) between canopy cover speed and soil water status during post-anthesis stage when the rain amount is limited in 2013. We may suggest that rapid canopy cover lead to negative effect on soil water status via higher transpiration if the rain is limited during post-anthesis stage of wheat.

scholarly journals Variability of grain yield components of some new winter wheat genotypes (Triticum aestivum L.)

2011 ◽  
Vol 48 (No. 5) ◽  
pp. 230-235
Author(s):  
M. Sabo ◽  
M. Bede ◽  
Ž.U. Hardi
Keyword(s):  
Winter Wheat ◽  
Grain Yield ◽  
Yield Components ◽  
Vegetation Period ◽  
Grain Number ◽  
Potential Yield ◽  
High Positive Correlation ◽  
Wheat Genotypes ◽  
Grain Number Per Spike ◽  
Grain Yield Components

Variability of grain yield components of some new winter wheat genotypes (e.g. Lara, Lenta, Kruna, Fiesta, Perla, and one line of AG-45) was examined. The analysis of grain yield components of these genotypes and the line was undertaken in a two-year research (1997/1998 and 1998/1999) at two different locations. Significant differences among genotypes, locations and research years were established. In the first experimental year (1997/1998) there was a high positive correlation between nearly all components of the grain yield. The most significant correlation was found between the grain number per spike and grain yield. In the second experimental year (1998/1999) the components did not show statistically significant correlation with the grain yield. It seems that the grain yield of examined genotypes depended significantly on the grain number per spike, grain mass per spike, and agroecological conditions during the vegetation period, whereby the potential yield was determined by the interaction among genotypes, location and production year. The biggest differences among examined genotypes of winter wheat were found in the stem height and spike length.

scholarly journals Assessment of drought resistance in pearl millet (Pennisetum americanum (L.) Leeke). II. Estimation of genotype response to stress

1987 ◽  
Vol 38 (1) ◽  
pp. 49 ◽  
Author(s):  
FR Bidinger ◽  
V Mahalakshmi ◽  
GDP Rao
Keyword(s):  
Drought Stress ◽  
Grain Yield ◽  
Pearl Millet ◽  
Selection Index ◽  
Yield Potential ◽  
Drought Response ◽  
Grain Number ◽  
Component Structure ◽  
Panicle Number ◽  
Response To Stress

The finding that the more than 50% of the variation in grain yield of pearl millet breeding lines in two different drought stress treatments could be attributed to variation in yield potential and time of flowering was used to develop a drought-response index (DRI) based on the residual variation in grain yield, adjusted for experimental error. DRI was positively correlated to measured yield in the drought treatments, and independent of both yield potential and time to flowering. DRI in both midseason and terminal stress treatments was unrelated to yield component structure in the irrigated control treatment, indicating that selection for plant type under non-stressed conditions will not influence drought response. DRI was correlated to both grain number per panicle, and grain yield per panicle in both stress treatments, suggesting differential ability to maintain normal grain number, and/or that grain yield per panicle was an important factor in response to stress. Maintenance of panicle number did not seem to be important for maintenance of yield under drought stress. The correlation of DRI and individual panicle yield was of sufficient magnitude for the latter to serve as a selection index in terminal stress. The use of a DRI as a component of breeding for better adaptation to stress is discussed.

Combining high grain number and weight through a DH-population to improve grain yield potential of wheat in high-yielding environments

2013 ◽  
Vol 145 ◽  
pp. 106-115 ◽  
Author(s):  
Daniela V. Bustos ◽  
Ahmed K. Hasan ◽  
Matthew P. Reynolds ◽  
Daniel F. Calderini
Keyword(s):  
Grain Yield ◽  
Yield Potential ◽  
Grain Number ◽  
Dh Population

scholarly journals Correlation, Regression and Path analysis among yield and yield traits in Triticum dicoccum

1970 ◽  
pp. 24-27
Author(s):  
Maysoun M. Saleh ◽  
Dyab S. Moussa ◽  
Nader I. Alkaraki ◽  
Abbas Lateef Abdurahman
Keyword(s):  
Grain Yield ◽  
Path Analysis ◽  
Selection Criteria ◽  
Agricultural Research ◽  
Grain Weight ◽  
Individual Plant ◽  
Grain Number ◽  
Triticum Dicoccum ◽  
Grain Number Per Spike ◽  
Significant Regression

Seven wheat genotypes (Triticum dicoccum) and the local check sham5 were planted all at Al-Ghab and Izra Research centers in The General Commission for Scientific Agricultural Research in Syria during growing season 2010/2011. Yield components (number of total and fertile tillers per plant, number and weight of grains per spike, weight of thousand grain and individual plant grain yield) were studied in two sites in order to predict their effect and to determine their effects on grain yield in order to define selection criteria for grain yield. Results revealed all studied traits except total tillers number were positively correlated with grain yield, and only (fertile tillers number and grain number per spike and grain weight per spike) had a significant regression with grain yield and these traits can explain about (27.6, 67.7, 62.2)% respectively of the variation final grain yield. Results of path analysis indicated that the direct effect of fertile tillers per plant and grain weight per spike on grain yield was positive and high (0.6178, 0.7563) respectively, so that we can depend on them in breeding program as selection criteria to increase grain yield in plant.

scholarly journals Identification and characterization of a natural polymorphism in FT-A2 associated with increased number of grains per spike in wheat

2021 ◽  
Author(s):  
Priscilla Glenn ◽  
Junli Zhang ◽  
Gina Brown-Guedira ◽  
Noah DeWitt ◽  
Jason P. Cook ◽  
...  
Keyword(s):  
Grain Yield ◽  
Durum Wheat ◽  
Tetraploid Wheat ◽  
Low Frequency ◽  
Yield Component ◽  
Loss Of Function ◽  
Grain Number ◽  
Wheat Varieties ◽  
Positive Effects ◽  
Grain Number Per Spike

Abstract Key message We discovered a natural FT-A2 allele that increases grain number per spike in both pasta and bread wheat with limited effect on heading time. Abstract Increases in wheat grain yield are necessary to meet future global food demands. A previous study showed that loss-of-function mutations in FLOWERING LOCUS T2 (FT2) increase spikelet number per spike (SNS), an important grain yield component. However, these mutations were also associated with reduced fertility, offsetting the beneficial effect of the increases in SNS on grain number. Here, we report a natural mutation resulting in an aspartic acid to alanine change at position 10 (D10A) associated with significant increases in SNS and no negative effects on fertility. Using a high-density genetic map, we delimited the SNS candidate region to a 5.2-Mb region on chromosome 3AS including 28 genes. Among them, only FT-A2 showed a non-synonymous polymorphism (D10A) present in two different populations segregating for the SNS QTL on chromosome arm 3AS. These results, together with the known effect of the ft-A2 mutations on SNS, suggest that variation in FT-A2 is the most likely cause of the observed differences in SNS. We validated the positive effects of the A10 allele on SNS, grain number, and grain yield per spike in near-isogenic tetraploid wheat lines and in an hexaploid winter wheat population. The A10 allele is present at very low frequency in durum wheat and at much higher frequency in hexaploid wheat, particularly in winter and fall-planted spring varieties. These results suggest that the FT-A2 A10 allele may be particularly useful for improving grain yield in durum wheat and fall-planted common wheat varieties.

scholarly journals Glutacetine® Biostimulant Applied on Wheat under Contrasting Field Conditions Improves Grain Number Leading to Better Yield, Upgrades N-Related Traits and Changes Grain Ionome

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 456
Author(s):  
Victor Maignan ◽  
Patrick Géliot ◽  
Jean-Christophe Avice
Keyword(s):  
Grain Yield ◽  
Temperate Climate ◽  
Grain Number ◽  
N Losses ◽  
Total N ◽  
P Content ◽  
Grain Number Per Spike ◽  
Grain Yield Components ◽  
Use Efficiency ◽  
Mn Content

Wheat is one of the most important cereals for human nutrition, but nitrogen (N) losses during its cultivation cause economic problems and environmental risks. In order to improve N use efficiency (NUE), biostimulants are increasingly used. The present study aimed to evaluate the effects of Glutacetine®, a biostimulant sprayed at 5 L ha−1 in combination with fertilizers (urea or urea ammonium nitrate (UAN)), on N-related traits, grain yield components, and the grain quality of winter bread wheat grown at three field sites in Normandy (France). Glutacetine® improved grain yield via a significant increase in the grain number per spike and per m2, which also enhanced the thousand grain weight, especially with urea. The total N in grains and the NUE tended to increase in response to Glutacetine®, irrespective of the site or the form of N fertilizer. Depending on the site, spraying Glutacetine® can also induce changes in the grain ionome (analyzed by X-ray fluorescence), with a reduction in P content observed (site 2 under urea nutrition) or an increase in Mn content (site 3 under UAN nutrition). These results provide a roadmap for utilizing Glutacetine® biostimulant to enhance wheat production and flour quality in a temperate climate.

scholarly journals Effects of Sowing Time and Growing Density on Agronomic Traits, Grain Yield, and Grain Quality of Waxy Sorghum Cultivar Hongliangfeng 1

2019 ◽  
Vol 11 (6) ◽  
pp. 12
Author(s):  
Can Wang ◽  
Lingbo Zhou ◽  
Guobing Zhang ◽  
Yan Xu ◽  
Xu Gao ◽  
...  
Keyword(s):  
Grain Yield ◽  
Amylose Content ◽  
Starch Content ◽  
Grain Weight ◽  
Grain Number ◽  
Sowing Time ◽  
Grain Number Per Spike ◽  
Weight Protein ◽  
1000 Grain Weight

The aim of this study was to determine the effects of sowing time and growing density on the yield and quality of grain in waxy sorghum (Sorghum bicolor L. Moench). The main plots were two sowing time: early sowing (5 April) and late sowing (20 April), and the subplots were three growing densities: 0.8 × 105, 1.1 × 105, and 1.4 × 105 plants/ha. Results showed that sowing time and growing density had significant effects on grain yield and grain quality of waxy sorghum cultivar Hongliangfeng 1. Grain yield, plant height, spike length, culm diameter, grain number per spike, grain weight per plant, 1000-grain weight, protein content, starch content, and amylopectin content were reduced by a delay of sowing time, while the tannin content and amylose content were increased by a delay of sowing time. Grain yield, plant height, spike length, culm diameter, grain number per spike, grain weight per plant, 1000-grain weight, protein content, starch content, and amylopectin content increased and then decreased with the increase of growing density, while the tannin content and amylose content decreased and then increased with the increase of growing density. These results hinted that appropriate sowing time and growing density are key cultivation measures to ensure high yield and good quality in waxy sorghum production.

scholarly journals TaLAMP1 Plays Key Roles in Plant Architecture and Yield Response to Nitrogen Fertilizer in Wheat

2021 ◽  
Vol 11 ◽  
Author(s):  
Ji Shi ◽  
Yiping Tong
Keyword(s):  
Field Experiment ◽  
Grain Yield ◽  
Plant Architecture ◽  
N Fertilizer ◽  
Yield Response ◽  
N Availability ◽  
Grain Number ◽  
N Concentration ◽  
Grain Number Per Spike ◽  
Spike Number

Understanding the molecular mechanisms in wheat response to nitrogen (N) fertilizer will help us to breed wheat varieties with improved yield and N use efficiency. Here, we cloned TaLAMP1-3A, -3B, and -3D, which were upregulated in roots and shoots of wheat by low N availability. In a hydroponic culture, lateral root length and N uptake were decreased in both overexpression and knockdown of TaLAMP1 at the seedling stage. In the field experiment with normal N supply, the grain yield of overexpression of TaLAMP1-3B is significantly reduced (14.5%), and the knockdown of TaLAMP1 was significantly reduced (15.5%). The grain number per spike of overexpression of TaLAMP1-3B was significantly increased (7.2%), but the spike number was significantly reduced (19.2%) compared with wild type (WT), although the grain number per spike of knockdown of TaLAMP1 was significantly decreased (15.3%), with no difference in the spike number compared with WT. Combined with the agronomic data from the field experiment of normal N and low N, both overexpression and knockdown of TaLAMP1 inhibited yield response to N fertilizer. Overexpressing TaLAMP1-3B greatly increased grain N concentration with no significant detrimental effect on grain yield under low N conditions; TaLAMP1-3 B is therefore valuable in engineering wheat for low input agriculture. These results suggested that TaLAMP1 is critical for wheat adaptation to N availability and in shaping plant architecture by regulating spike number per plant and grain number per spike. Optimizing TaLAMP1 expression may facilitate wheat breeding with improved yield, grain N concentration, and yield responses to N fertilizer.

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