scholarly journals Two Plastid Fatty Acid Exporters Contribute to Seed Oil Accumulation in Arabidopsis

2020 ◽  
Vol 182 (4) ◽  
pp. 1910-1919 ◽  
Author(s):  
Nannan Li ◽  
Hongjun Meng ◽  
Shengting Li ◽  
Zhen Zhang ◽  
Xin Zhao ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Seed Oil ◽  
Oil Accumulation

scholarly journals Dynamic Transcriptome Changes Related to Oil Accumulation in Developing Soybean Seeds

2019 ◽  
Vol 20 (9) ◽  
pp. 2202 ◽  
Author(s):  
Songnan Yang ◽  
Long Miao ◽  
Jianbo He ◽  
Kai Zhang ◽  
Yan Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Candidate Genes ◽  
Molecular Basis ◽  
Oil Content ◽  
Seed Oil ◽  
Soybean Seed ◽  
Soybean Seeds ◽  
Seed Oil Content ◽  
Oil Accumulation ◽  
Expression Levels

Soybean is one of the most important oil crops in the world. Revealing the molecular basis and exploring key candidate genes for seed oil synthesis has great significance for soybean improvement. In this study, we found that oil accumulation rates and gene expression levels changed dynamically during soybean seed development. The expression levels of genes in metabolic pathways such as carbon fixation, photosynthesis, glycolysis, and fatty acid biosynthesis were significantly up-regulated during the rapid accumulation of oil in developing soybean seeds. Through weighted correlation network analysis, we identified six co-expression modules associated with soybean seed oil content and the pink module was the most positively correlated (r = 0.83, p = 7 × 10−4) network. Through the integration of differential expression and co-expression analysis, we predicted 124 candidate genes potentially affecting soybean seed oil content, including seven genes in lipid metabolism pathway, two genes involved in glycolysis, one gene in sucrose metabolism, and 12 genes belonged to transcription factors as well as other categories. Among these, three genes (GmABI3b, GmNFYA and GmFAD2-1B) have been shown to control oil and fatty acid content in soybean seeds, and other newly identified candidate genes would broaden our knowledge to understand the molecular basis for oil accumulation in soybean seeds.

scholarly journals The WRKY6 transcription factor affects seed oil accumulation and alters fatty acid compositions in Arabidopsis thaliana

2020 ◽  
Vol 169 (4) ◽  
pp. 612-624 ◽  
Author(s):  
Ge Song ◽  
Xueping Li ◽  
Raheel Munir ◽  
Ali Raza Khan ◽  
Wardah Azhar ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Fatty Acid ◽  
Seed Oil ◽  
Oil Accumulation ◽  
Fatty Acid Compositions

Nitric oxide affects seed oil accumulation and fatty acid composition through protein S-nitrosation

2020 ◽  
Author(s):  
Jing Liu ◽  
Xiao-yi Zhu ◽  
Lin-Bin Deng ◽  
Hong-Fang Liu ◽  
Jun Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Stress Responses ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Oil Accumulation ◽  
No Donor

Abstract Nitric oxide (NO) is a key signaling molecule regulating several plant developmental and stress responses. Here, we report that NO plays an important role in seed oil content and fatty acid composition. RNAi silencing of Arabidopsis S-nitrosoglutathione reductase 1 (GSNOR1) led to reduced seed oil content. In contrast, nitrate reductase double mutant nia1nia2 had increased seed oil content, compared with wild-type plants. Moreover, the concentrations of palmitic acid (C16:0), linoleic acid (C18:2), and linolenic acid (C18:3) were higher, whereas those of stearic acid (C18:0), oleic acid (C18:1), and arachidonic acid (C20:1) were lower, in seeds of GSNOR1 RNAi lines. Similar results were obtained with rapeseed embryos cultured in vitro with the NO donor sodium nitroprusside (SNP), and the NO inhibitor NG-Nitro-L-arginine Methyl Ester (L-NAME). Compared with non-treated embryos, the oil content decreased in SNP-treated embryos, and increased in L-NAME-treated embryos. Relative concentrations of C16:0, C18:2 and C18:3 were higher, whereas C18:1 concentration decreased in rapeseed embryos treated with SNP. Proteomics and transcriptome analysis revealed that three S-nitrosated proteins and some key genes involved in oil synthesis, were differentially regulated in SNP-treated embryos. Therefore, regulating NO content could be a novel approach to increasing seed oil content in cultivated oil crops.

Heat Stress Suppresses Brassica napus Seed Oil Accumulation by Inhibition of Photosynthesis and BnWRI1 Pathway

2019 ◽  
Vol 60 (7) ◽  
pp. 1457-1470 ◽  
Author(s):  
Ruizhi Huang ◽  
Zhihong Liu ◽  
Meiqing Xing ◽  
Yong Yang ◽  
Xuelong Wu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Seed Oil ◽  
Fatty Acid Biosynthesis ◽  
De Novo ◽  
Sugar Content ◽  
Light Response ◽  
Acid Biosynthesis ◽  
Oil Accumulation

Abstract Heat stress during Brassica napus seed filling severely impairs yield and oil content. However, the mechanisms underlying heat-stress effects on B. napus seed photosynthesis and oil accumulation remain elusive. In this study, we showed that heat stress resulted in reduction of seed oil accumulation, whereas the seed sugar content was enhanced, which indicated that incorporation of carbohydrates into triacylglycerols was impaired. Photosynthesis and respiration rates, and the maximum quantum yield of photosystem II in developing seeds were inhibited by heat stress. Transcriptome analysis revealed that heat stress led to up-regulation of genes associated with high light response, providing evidence that photoinhibition was induced by heat stress. BnWRI1 and its downstream genes, including genes involved in de novo fatty acid biosynthesis pathway, were down-regulated by heat stress. Overexpression of BnWRI1 with a seed-specific promoter stabilized both oil accumulation and photosynthesis under the heat-stress condition, which suggested BnWRI1 plays an important role in mediating the effect of heat stress on fatty acid biosynthesis. A number of sugar transporter genes were inhibited by heat stress, resulting in defective integration of carbohydrates into triacylglycerols units. The results collectively demonstrated that disturbances of the seed photosynthesis machinery, impairment of carbohydrates incorporation into triacylglycerols and transcriptional deregulation of the BnWRI1 pathway by heat stress might be the major cause of decreased oil accumulation in the seed.

scholarly journals A Tree Peony Trihelix Transcription Factor PrASIL1 Represses Seed Oil Accumulation

2021 ◽  
Vol 12 ◽  
Author(s):  
Weizong Yang ◽  
Jiayuan Hu ◽  
Jyoti R. Behera ◽  
Aruna Kilaru ◽  
Yanping Yuan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Transcription Factor ◽  
Fatty Acid ◽  
Seed Oil ◽  
Unsaturated Fatty Acids ◽  
Fatty Acid Biosynthesis ◽  
Tree Peony ◽  
Oil Accumulation ◽  
Oil Biosynthesis ◽  
Trihelix Transcription Factor

In many higher plants, seed oil accumulation is governed by complex multilevel regulatory networks including transcriptional regulation, which primarily affects fatty acid biosynthesis. Tree peony (Paeonia rockii), a perennial deciduous shrub endemic to China is notable for its seed oil that is abundant in unsaturated fatty acids. We discovered that a tree peony trihelix transcription factor, PrASIL1, localized in the nucleus, is expressed predominantly in developing seeds during maturation. Ectopic overexpression of PrASIL1 in Nicotiana benthamiana leaf tissue and Arabidopsis thaliana seeds significantly reduced total fatty acids and altered the fatty acid composition. These changes were in turn associated with the decreased expression of multitudinous genes involved in plastidial fatty acid synthesis and oil accumulation. Thus, we inferred that PrASIL1 is a critical transcription factor that represses oil accumulation by down-regulating numerous key genes during seed oil biosynthesis. In contrary, up-regulation of oil biosynthesis genes and a significant increase in total lipids and several major fatty acids were observed in PrASIL1-silenced tree peony leaves. Together, these results provide insights into the role of trihelix transcription factor PrASIL1 in controlling seed oil accumulation. PrASIL1 can be targeted potentially for oil enhancement in tree peony and other crops through gene manipulation.

scholarly journals A comparative study on quality parameters of pumpkin, melon and sunflower oils during thermal treatment

OCL ◽  
2019 ◽  
Vol 26 ◽  
pp. 32 ◽  
Author(s):  
Zhana Petkova ◽  
Ginka Antova
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Thermal Treatment ◽  
Microwave Heating ◽  
Acid Composition ◽  
Seed Oil ◽  
Conventional Heating ◽  
Melon Seed ◽  
Sunflower Oils ◽  
Melon Seed Oil

Current paper reveals the impact of thermal treatment on the quality of two seed oils – pumpkin and melon compared to the quality of the most used oil – sunflower oil. Conventional and microwave heating were used for processing the oils. The duration of the thermal treatment was 9, 12 and 18 min for the conventional heating. The microwave heating was performed with two microwave powers of the equipment (600 W and 900 W) for 3, 6, 9 and 12 min. At every stage of the thermal processing were determined acid and peroxide value, the absorbance of the oils at 232 and 268 nm, tocopherol and fatty acid composition. It was observed that the degree of oxidation of the examined oils during microwave and conventional heating increased with the duration of the thermal process and the power of the microwaves. Also, the two methods of heating had a little impact on the processes leading to the formation of free fatty acids. Total tocopherols of the melon seed oil were more stable to thermal treatment. The amount of linoleic acid decreased in the pumpkin and sunflower oils during microwave treatment, while that of oleic and palmitic acid relatively increased. The biggest change in the fatty acid composition of both oils was found during microwave heating at 900W. The changes in fatty acid composition of thermally treated melon seed oil were insignificant. Overall, melon seed oil was observed to be more thermally stable than pumpkin and sunflower oils.

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