scholarly journals Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography

2021 ◽  
Vol 77 (5) ◽  
pp. 628-644
Author(s):  
Gabrielle Illava ◽  
Richard Jayne ◽  
Aaron D. Finke ◽  
David Closs ◽  
Wenjie Zeng ◽  
...  
Keyword(s):  
Data Collection ◽  
Integrated System ◽  
Ease Of Use ◽  
Time Varying ◽  
Fixed Target ◽  
Sample Handling ◽  
Small Crystals ◽  
Fixed Orientation ◽  
Common Problems ◽  
Liquid Flows

Serial synchrotron crystallography (SSX) is enabling the efficient use of small crystals for structure–function studies of biomolecules and for drug discovery. An integrated SSX system has been developed comprising ultralow background-scatter sample holders suitable for room and cryogenic temperature crystallographic data collection, a sample-loading station and a humid `gloveless' glovebox. The sample holders incorporate thin-film supports with a variety of designs optimized for different crystal-loading challenges. These holders facilitate the dispersion of crystals and the removal of excess liquid, can be cooled at extremely high rates, generate little background scatter, allow data collection over >90° of oscillation without obstruction or the risk of generating saturating Bragg peaks, are compatible with existing infrastructure for high-throughput cryocrystallography and are reusable. The sample-loading station allows sample preparation and loading onto the support film, the application of time-varying suction for optimal removal of excess liquid, crystal repositioning and cryoprotection, and the application of sealing films for room-temperature data collection, all in a controlled-humidity environment. The humid glovebox allows microscope observation of the sample-loading station and crystallization trays while maintaining near-saturating humidities that further minimize the risks of sample dehydration and damage, and maximize working times. This integrated system addresses common problems in obtaining properly dispersed, properly hydrated and isomorphous microcrystals for fixed-orientation and oscillation data collection. Its ease of use, flexibility and optimized performance make it attractive not just for SSX but also for single-crystal and few-crystal data collection. Fundamental concepts that are important in achieving desired crystal distributions on a sample holder via time-varying suction-induced liquid flows are also discussed.

Processing of Histopathological Data

1971 ◽  
Vol 10 (03) ◽  
pp. 142-147
Author(s):  
M. RENAUD ◽  
M. AQARQ ◽  
R. GERARD-MARCHANT ◽  
M. WOLFF-TERROINE
Keyword(s):  
Integrated System ◽  
Ease Of Use ◽  
Financial Data ◽  
Identification Number ◽  
Processing Data ◽  
Cancer Hospital

A method is presented for processing data from the histopathological laboratory of a cancer hospital. Emphasis is laid on the ease of use, the connection of medical, administrative and financial data, and the strictness of control of patient’s identification number. The system can be used separately; it is also a module for a large integrated system covering all the activities of the hospital.

scholarly journals Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ki Hyun Nam ◽  
Jihan Kim ◽  
Yunje Cho
Keyword(s):  
Data Collection ◽  
Room Temperature ◽  
Picosecond Laser ◽  
Temperature Structure ◽  
Sample Holder ◽  
Fixed Target ◽  
Random Orientation ◽  
Physical Damage ◽  
Crystal Sample ◽  
Serial Crystallography

AbstractThe serial crystallography (SX) technique enables the determination of the room-temperature structure of a macromolecule while causing minimal radiation damage, as well as the visualization of the molecular dynamics by time-resolved studies. The fixed-target (FT) scanning approach is one method for SX sample delivery that minimizes sample consumption and minimizes physical damage to crystals during data collection. Settling of the crystals on the sample holder in random orientation is important for complete three dimensional data collection. To increase the random orientation of crystals on the sample holder, we developed a polyimide mesh-based sample holder with irregular crystal mounting holes for FT-SX. The polyimide mesh was fabricated using a picosecond laser. Each hole in the polyimide mesh has irregularly shaped holes because of laser thermal damage, which may cause more crystals to settle at random orientations compared to regular shaped sample holders. A crystal sample was spread onto a polyimide-mesh, and a polyimide film was added to both sides to prevent dehydration. Using this sample holder, FT-SX was performed at synchrotron and determined the room-temperature lysozyme structure at 1.65 Å. The polyimide mesh with irregularly shaped holes will allow for expanded applications in sample delivery for FT-SX experiments.

INOVASI APLIKASI TRANSPORTASIKU DI DINAS PERHUBUNGAN KOTA SURABAYA

Publika ◽  
2021 ◽  
pp. 199-214
Author(s):  
Kholilah Danarratoe Kharima ◽  
Eva Hany Fanida
Keyword(s):  
Data Collection ◽  
Public Service ◽  
Public Transportation ◽  
Community Support ◽  
Information Services ◽  
Ease Of Use ◽  
The Public ◽  
Citizen Support ◽  
Information Reliability

Penggunaan teknologi saat ini dibutuhkan dalam bidang pelayanan publik. Seiring dengan bertambahnya kebutuhan masyarakat maka pemerintah sebagai penyedia layanan publik terus melakukan pembaruan terhadap perubahan pelayanan yang diberikan agar memudahkan masyarakat dalam mendapatkan layanan informasi. Salah satu penerapan e-government oleh Dinas Perhubungan Kota Surabaya adalah inovasi aplikasi TransportasiKu yang bertujuan untuk memberikan layanan informasi terkait lalu lintas dan transportasi umum yang ada di Surabaya secara terkini, praktis, lebih cepat dengan satu aplikasi yang memuat semua informasi melalui genggaman smartphone. Penelitian ini menganalisa menggunakan indikator measuring the quality of e-government from the user prespective (Kozák, 2018) dengan enam indikator yaitu ease of use, content and appearance of the information, reliability, citizen support, trust/security, support in completing forms. Penelitian ini menggunakan jenis penelitian deskriptif dengan metode kualitatif, teknik pengumpulan data wawancara, observasi, dokumentasi dan teknik analisa data yaitu pengumpulan data, reduksi data, penyajian data dan penarikan kesimpulan/verifikasi. Hasil penelitian ini menunjukkan bahwa aplikasi ini mendapat respon yang cukup baik dari masyarakat sebagai pengguna karena dapat memberikan layanan informasi yang dibutuhkan namun masih ada juga gangguan dalam penggunaannya. Indikator pertama, kemudahan penggunaan dirasa para pengguna sangat mudah digunakan untuk setiap saat. Indikator kedua, isi dan tampilan informasi yang dihadirkan sederhana dan lengkap. Indikator ketiga keandalan, pada aplikasi ini server yang digunakan masih kurang cukup. Indikator keempat dukungan masyarakat untuk halaman bantuan tidak tersedia hanya ada kolom komentar. Indikator kelima kepercayaan/keamanan, tidak menyertakan informasi pribadi sehingga aman. Indikator keenam dukungan dalam pengisian, formulir aplikasi ini berbasis informasi tanpa perlu pengisian formulir data para penggunanya. Kata Kunci : Pelayanan Publik, Inovasi, E-Government, TransportasiKu   The use of technology is currently needed in the field of public services. Along with the increasing needs of the community, the government as a public service provider continues to make updates to changes in the services provided to make it easier for the public to get information services. One of the applications of e-government by the Surabaya City Department of Transportation is the TransportationKu application innovation which aims to provide information services related to traffic and public transportation in Surabaya in an up-to-date, practical, faster way with one application that contains all information through the grip of a smartphone. This study analyzes using indicators measuring the quality of e-government from the user perspective (Kozák, 2018) with six indicators, namely ease of use, content and appearance of the information, reliability, citizen support, trust/security, support in completing forms. This research uses descriptive research with qualitative methods, interview data collection techniques, observation, documentation and data analysis techniques, namely data collection, data reduction, data presentation and conclusion / verification. The results of this study indicate that this application has received a fairly good response from the public as a user because it can provide the information services needed, but there are still disturbances in its use. The first indicator, ease of use, is felt by users to be very easy to use at any time. The second indicator, the content and display of the information presented is simple and complete. The third indicator is reliability, in this application the server used is still insufficient. The fourth indicator of community support for the help page is not available, only a comment column. The fifth indicator is trust / security, does not include personal information so it is safe. The sixth indicator of support in filling in this application form is information-based without the need to fill out user data forms. Keywords: Public Service, Innovation, E-Government, TransportasiKu

Abstract WMP68: Smartphone Support System for Mobile Imaging Display and Management of Acute Stroke

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Kristina Shkirkova ◽  
Eftitan Y Akam ◽  
Josephine F Huang ◽  
Sunil A Sheth ◽  
May Nour ◽  
...  
Keyword(s):  
Acute Stroke ◽  
Stroke Care ◽  
Final Diagnosis ◽  
Integrated System ◽  
Ease Of Use ◽  
Imaging Data ◽  
Acute Stroke Care ◽  
Team Members ◽  
Information Platform ◽  
Stroke Team

Introduction: Rapid dissemination and coordination of clinical and imaging data among multidisciplinary team members is essential for optimal acute stroke care. Standard desktop EMRs are ill-suited for this purpose, but mobile smartphone and tablet applications are highly promising platforms for accelerated, data-driven patient diagnosis and treatment. This study tested an advanced mobile integrated system for distribution of patient clinical and imaging information. Methods: We tested the iStroke/Synapse ERm system (Figure) for smartphone and tablet display and integration of clinical data, CT, MR, and catheter angiographic imaging, and real-time stroke team communications, in consecutive acute neurovascular patients at a Comprehensive Stroke Center. Results: From 5/2014 to 10/2014, the Synapse ERm application was installed and used by 33 stroke team members, in 84 Code Stroke ED patients. Patient age was 69.1 (±17.5), with 40.5% female. Final diagnosis was: ischemic stroke 66%, TIA 7%, ICH 6%, and CV mimic 21%. Each patient record was viewed on average 13 times by at least 3 team members. The most used feature was CT, MR and cath angio image display, viewed on average 4 times per patient by at least 2 users. In-app tweet team communications were sent by average 2 users per case and viewed by average 6 team members. Use of the system was associated with treatment times that exceeded national guideline targets for thrombolysis and endovascular thrombectomy, including door-to-needle 50 min (IQR 24-60) and door-to-groin 92 min (IQR 65-128). In user surveys, the mobile information platform was judged easy to employ in 91% of uses and of added help in stroke management in a substantial majority of cases. Conclusion: The Synapse ERm system, a smartphone/tablet platform for stroke team communication and distribution and integration of clinical and imaging data, showed high ease of use, substantial added management value, and association with rapid processes of care.

PENGARUH PERSEPSI RISIKO, MANFAAT, DAN KEMUDAHAN PENGGUNAAN

2021 ◽  
Vol 9 (1) ◽  
pp. 75-84
Author(s):  
Hana Zahra Salsabila
Keyword(s):  
Data Collection ◽  
Perceived Risk ◽  
Sampling Method ◽  
Ease Of Use ◽  
Purchasing Decisions ◽  
Purposive Sampling ◽  
Independent Variables ◽  
Significant Negative Effect ◽  
Economics Faculty ◽  
Negative Effect

This study aims to determine the effect of perceived risk, benefit and ease of use on purchasing decisions. This study uses four variables, namely risk, benefit and ease of use as independent variables, and purchasing decisions as the dependent variable. The population in this study were active students of the economics faculty at the Sarjanawiyata Tamansiswa Yogyakarta University who used the Shopee application, who were then sampled as many as 120 respondents. The sampling method using purposive sampling with data collection using a questionnaire. The results showed that the risks, benefits and ease of use simultaneously had a significant effect on online purchasing decisions at Shopee, risk had a significant negative effect on online purchasing decisions at Shopee, benefits had a significant negative effect on online purchasing decisions at Shopee, ease of use has a significant effect on online purchasing decisions at Shopee.

A Quantum NeuroIS Data Analytics Architecture for the Usability Evaluation of Learning Management Systems

2021 ◽  
pp. 416-434
Author(s):  
Raul Valverde ◽  
Beatriz Torres ◽  
Hamed Motaghi
Keyword(s):  
Data Collection ◽  
Computer Anxiety ◽  
Learning Management Systems ◽  
Past Research ◽  
Usability Evaluation ◽  
Ease Of Use ◽  
Quantum Model ◽  
Learning Management ◽  
Evaluation Of Learning ◽  
Electroencephalogram Eeg

NeuroIS uses tools such as electroencephalogram (EEG) that can be used to measure high brainwave frequencies that can be linked to human anxiety. Past research showed that computer anxiety influences how users perceive ease of use of a learning management system (LMS). Although computer anxiety has been used successfully to evaluate the usability of LMS, the main data collection mechanisms proposed for its evaluation have been questionnaires. Questionnaires suffer from possible problems such as being inadequate to understand some forms of information such as emotions and honesty in the responses. Quantum-based approaches to consciousness have been very popular in the last years including the quantum model reduction in microtubules of Penrose and Hameroff (1995). The objective of the chapter is to propose an architecture based on a NeuroIS that collects data by using EEG from users and then use the collected data to perform analytics by using a quantum consciousness model proposed for computer anxiety measurements for the usability testing of a LMS.

scholarly journals Determination of Starch, Including Maltooligosaccharides, in Animal Feeds: Comparison of Methods and a Method Recommended for AOAC Collaborative Study

2009 ◽  
Vol 92 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Mary B Hall
Keyword(s):  
Dry Matter ◽  
Collaborative Study ◽  
Ease Of Use ◽  
Acetate Buffer ◽  
Sample Handling ◽  
Animal Feeds ◽  
Heat Stable ◽  
Replacement Method ◽  
Aoac Method

Abstract Starch is a nutritionally important carbohydrate in feeds that is increasingly measured and used for formulation of animal diets. Discontinued production of the enzyme Rhozyme-S required for AOAC Method 920.40 invalidated this method for starch in animal feeds. The objective of this study was to compare methods for the determination of starch as potential candidates as a replacement method and for an AOAC collaborative study. Many starch methods are available, but they vary in accuracy, replicability, and ease of use. After assays were evaluated that differed in gelatinization method, number of reagents, and sample handling, and after assays with known methodological defects were excluded, 3 enzymaticcolorimetric assays were selected for comparison. The assays all used 2-stage, heat-stable, -amylase and amyloglucosidase hydrolyses, but they differed in the gelatinization solution (heating in water, 3-(N-morpholino) propanesulfonic acid buffer, or acetate buffer). The measured values included both starch and maltooligosaccharides. The acetate buffer-only method was performed in sealable vessels with dilution by weight; it gave greater starch values (26 percentage units of sample dry matter) in the analysis of feed/food substrates than did the other methods. This method is a viable candidate for a collaborative study.

scholarly journals Using Zoom Videoconferencing for Qualitative Data Collection: Perceptions and Experiences of Researchers and Participants

2019 ◽  
Vol 18 ◽  
pp. 160940691987459 ◽  
Author(s):  
Mandy M. Archibald ◽  
Rachel C. Ambagtsheer ◽  
Mavourneen G. Casey ◽  
Michael Lawless
Keyword(s):  
Mixed Methods ◽  
Data Collection ◽  
Information And Communication Technologies ◽  
Qualitative Data ◽  
Qualitative Interviews ◽  
Communication Technologies ◽  
Ease Of Use ◽  
Practice Nurses ◽  
Information And Communication ◽  
Conducting Research

Advances in communication technologies offer new opportunities for the conduct of qualitative research. Among these, Zoom—an innovative videoconferencing platform—has a number of unique features that enhance its potential appeal to qualitative and mixed-methods researchers. Although studies have explored the use of information and communication technologies for conducting research, few have explored both researcher and participant perspectives on the use of web and videoconferencing platforms. Further, data are lacking on the benefits and challenges of using Zoom as a data collection method. In this study, we explore the feasibility and acceptability of using Zoom to collect qualitative interview data within a health research context in order to better understand its suitability for qualitative and mixed-methods researchers. We asked 16 practice nurses who participated in online qualitative interviews about their experiences of using Zoom and concurrently recorded researcher observations. Although several participants experienced technical difficulties, most described their interview experience as highly satisfactory and generally rated Zoom above alternative interviewing mediums such as face-to-face, telephone, and other videoconferencing services, platforms, and products. Findings suggest the viability of Zoom as a tool for collection of qualitative data because of its relative ease of use, cost-effectiveness, data management features, and security options. Further research exploring the utility of Zoom is recommended in order to critically assess and advance innovations in online methods.

How a System for Reporting Medical Errors Can and Cannot Improve Patient Safety

2006 ◽  
Vol 72 (11) ◽  
pp. 1088-1091 ◽  
Author(s):  
John R. Clarke
Keyword(s):  
Patient Safety ◽  
Medical Errors ◽  
Systems Of Care ◽  
Improve Patient Safety ◽  
Integrated System ◽  
Ease Of Use ◽  
Near Misses ◽  
Safety Reporting ◽  
Ideal System ◽  
Reporting Systems

The Institute of Medicine has recommended systems for reporting medical errors. This article discusses the necessary components of patient safety databases, steps for implementing patient safety reporting systems, what systems can do, what they cannot do, and motivations for physician participation. An ideal system captures adverse events, when care harms patients, and near misses, when errors occur without any harm. Near misses signal system weaknesses and, because harm did not occur, may provide insight into solutions. With an integrated system, medical errors can be linked to patient and team characteristics. Confidentiality and ease of use are important incentives in reporting. Confidentiality is preferred to anonymity to allow follow-up. Analysis and feedback are critical. Reporting systems need to be linked to organizational leaders who can act on the conclusions of reports. The use of statistics is limited by the absence of reliable numerators and denominators. Solutions should focus on changing the cultural environment. Patient safety reporting systems can help bring to light, monitor, and correct systems of care that produces medical errors. They are useful components of the patient safety and quality improvement initiatives of healthcare systems and they warrant involvement by physicians.

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