scholarly journals Pilot study on the influence of cold atmospheric plasma on bacterial contamination and healing tendency of chronic wounds

2020 ◽  
Vol 18 (10) ◽  
pp. 1094-1101
Author(s):  
Maurice Moelleken ◽  
Finja Jockenhöfer ◽  
Cornelia Wiegand ◽  
Jan Buer ◽  
Sven Benson ◽  
...  
Keyword(s):  
Pilot Study ◽  
Bacterial Contamination ◽  
Chronic Wounds ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma

scholarly journals Prospective, Comparative Clinical Pilot Study of Cold Atmospheric Plasma Device in the Treatment of Atopic Dermatitis

2021 ◽  
Author(s):  
Young Jae Kim ◽  
Dong Jun Lim ◽  
Mi Young Lee ◽  
Woo Jin Lee ◽  
Sung Eun Chang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Pilot Study ◽  
Severity Index ◽  
Skin Lesions ◽  
Treated Group ◽  
Atmospheric Plasma ◽  
Clinical Severity ◽  
Control Treatment ◽  
Cold Atmospheric Plasma ◽  
Plasma Device

Abstract Introduction: Cold atmospheric plasma generates free radicals through the ionization of air at room temperature. Its effect and safety profile in patients with atopic dermatitis have not been evaluated prospectively.Objective: We aimed to investigate the effect and safety of cold atmospheric plasma in patients with atopic dermatitis with a prospective pilot study.Methods: Cold atmospheric plasma treatment or sham control treatment were applied respectively in randomly assigned and symmetric skin lesions. Three treatment sessions were performed at weeks 0, 1, and 2. Clinical severity indices were assessed at weeks 0, 1, 2, and 4 after treatment. Additionally, the microbial characteristics of the lesions before and after treatments were analyzed.Results: We included 22 patients with mild to moderate atopic dermatitis presented with symmetric lesions. We found that cold atmospheric plasma can alleviate the clinical severity of atopic dermatitis. Modified atopic dermatitis antecubital severity and eczema area and severity index score were significantly decreased in the treated group. Furthermore, scoring of atopic dermatitis score and pruritic visual analog scales significantly improved. In microbiome analysis revealed significantly reduced proportion of Staphylococcus aureus in the treated group.Conclusion: Cold atmospheric plasma can significantly improve mild and moderate atopic dermatitis without safety issues.

scholarly journals The Response and Tolerability of a Novel Cold Atmospheric Plasma Wound Dressing for the Healing of Split Skin Graft Donor Sites: A Controlled Pilot Study

2021 ◽  
pp. 1-9
Author(s):  
Annika van Welzen ◽  
Matti Hoch ◽  
Philip Wahl ◽  
Frank Weber ◽  
Susen Rode ◽  
...  
Keyword(s):  
Wound Healing ◽  
Pilot Study ◽  
Skin Graft ◽  
Wound Dressing ◽  
Atmospheric Plasma ◽  
Split Skin Graft ◽  
Cold Atmospheric Plasma ◽  
Positive Effects ◽  
Graft Donor ◽  
Split Skin

<b><i>Introduction:</i></b> Cold atmospheric plasma (CAP) has positive effects on wound healing and antimicrobial properties. However, an ongoing challenge is the development of specific modes of application for different clinical indications. <b><i>Objectives:</i></b> We investigated in a prospective pilot study the response and tolerability of a newly developed CAP wound dressing for the acute healing of split skin graft donor sites compared to conventional therapy. <b><i>Methods:</i></b> We applied both treatments to each patient (<i>n</i> = 10) for 7 days and measured 4 parameters of wound healing every other day (i.e., 1,440 measurements) using a hyperspectral imaging camera. Additionally, we evaluated the clinical appearance and pain levels reported by the patients. <b><i>Results:</i></b> The CAP wound dressing was superior to the control (<i>p</i> &#x3c; 0.001) in the improvement of 3 wound parameters, that is, deep tissue oxygen saturation, hemoglobin distribution, and tissue water distribution. CAP was well tolerated, and pain levels were lower in CAP-treated wound areas. <b><i>Conclusion:</i></b> CAP wound dressing is a promising new tool for acute wound healing.

The repetitive application of cold atmospheric plasma (CAP) improves microcirculation parameters in chronic wounds

2021 ◽  
pp. 104220
Author(s):  
Jan-Oluf Jensen ◽  
Lysann Schulz ◽  
Sophie Schleusser ◽  
Nico Matzkeit ◽  
Felix H. Stang ◽  
...  
Keyword(s):  
Chronic Wounds ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma

scholarly journals The Potential Use of a Cold Atmospheric Plasma Jet for Decontamination of Hospital Surfaces. A Pilot Study

2021 ◽  
Author(s):  
Muireann Fallon ◽  
Sarah Kennedy ◽  
Sharath Kumar ◽  
Stephen Daniels ◽  
Hilary Humphreys
Keyword(s):  
Pilot Study ◽  
Plasma Jet ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Potential Use

scholarly journals Effect of Cold Atmospheric Plasma on Epigenetic Changes, DNA Damage, and Possibilities for Its Use in Synergistic Cancer Therapy

2021 ◽  
Vol 22 (22) ◽  
pp. 12252
Author(s):  
Dušan Braný ◽  
Dana Dvorská ◽  
Ján Strnádel ◽  
Tatiana Matáková ◽  
Erika Halašová ◽  
...  
Keyword(s):  
Dna Damage ◽  
Chronic Wounds ◽  
Skin Diseases ◽  
Modern Medicine ◽  
Atmospheric Plasma ◽  
Tumour Mass ◽  
Epigenetic Changes ◽  
Cold Atmospheric Plasma ◽  
Non Coding Rnas ◽  
Expression And Activity

Cold atmospheric plasma has great potential for use in modern medicine. It has been used in the clinical treatment of skin diseases and chronic wounds, and in laboratory settings it has shown effects on selective decrease in tumour-cell viability, reduced tumour mass in animal models and stem-cell proliferation. Many researchers are currently focusing on its application to internal structures and the use of plasma-activated liquids in tolerated and effective human treatment. There has also been analysis of plasma’s beneficial synergy with standard pharmaceuticals to enhance their effect. Cold atmospheric plasma triggers various responses in tumour cells, and this can result in epigenetic changes in both DNA methylation levels and histone modification. The expression and activity of non-coding RNAs with their many important cell regulatory functions can also be altered by cold atmospheric plasma action. Finally, there is ongoing debate whether plasma-produced radicals can directly affect DNA damage in the nucleus or only initiate apoptosis or other forms of cell death. This article therefore summarises accepted knowledge of cold atmospheric plasma’s influence on epigenetic changes, the expression and activity of non-coding RNAs, and DNA damage and its effect in synergistic treatment with routinely used pharmaceuticals.

Cold atmospheric plasma, the removal of blood from steel and its effect on staphylococcal biofilm formation. A pilot study

2020 ◽  
Vol 19-20 ◽  
pp. 100104
Author(s):  
Muireann Fallon ◽  
Maria Boyle ◽  
Sarah Kennedy ◽  
Stephen Daniels ◽  
Hilary Humphreys
Keyword(s):  
Pilot Study ◽  
Biofilm Formation ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma

scholarly journals Cold Atmospheric Plasma: A Powerful Tool for Modern Medicine

2020 ◽  
Vol 21 (8) ◽  
pp. 2932 ◽  
Author(s):  
Dušan Braný ◽  
Dana Dvorská ◽  
Erika Halašová ◽  
Henrieta Škovierová
Keyword(s):  
Chronic Wounds ◽  
Modern Medicine ◽  
Atmospheric Plasma ◽  
Microbial Infection ◽  
Negative Effects ◽  
Cold Atmospheric Plasma ◽  
Internal Structures ◽  
Wide Range

Cold atmospheric plasma use in clinical studies is mainly limited to the treatment of chronic wounds, but its application in a wide range of medical fields is now the goal of many analyses. It is therefore likely that its application spectrum will be expanded in the future. Cold atmospheric plasma has been shown to reduce microbial load without any known significant negative effects on healthy tissues, and this should enhance its possible application to any microbial infection site. It has also been shown to have anti-tumour effects. In addition, it acts proliferatively on stem cells and other cultivated cells, and the highly increased nitric oxide levels have a very important effect on this proliferation. Cold atmospheric plasma use may also have a beneficial effect on immunotherapy in cancer patients. Finally, it is possible that the use of plasma devices will not remain limited to surface structures, because current endeavours to develop sufficiently miniature microplasma devices could very likely lead to its application in subcutaneous and internal structures. This study summarises the available literature on cold plasma action mechanisms and analyses of its current in vivo and in vitro use, primarily in the fields of regenerative and dental medicine and oncology.

scholarly journals Prospective, comparative clinical pilot study of cold atmospheric plasma device in the treatment of atopic dermatitis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Young Jae Kim ◽  
Dong Jun Lim ◽  
Mi Young Lee ◽  
Woo Jin Lee ◽  
Sung Eun Chang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Pilot Study ◽  
Severity Index ◽  
Skin Lesions ◽  
Treated Group ◽  
Atmospheric Plasma ◽  
Clinical Severity ◽  
Control Treatment ◽  
Cold Atmospheric Plasma ◽  
Plasma Device

AbstractCold atmospheric plasma generates free radicals through the ionization of air at room temperature. Its effect and safety profile as a treatment modality for atopic dermatitis lesions have not been evaluated prospectively enough. We aimed to investigate the effect and safety of cold atmospheric plasma in patients with atopic dermatitis with a prospective pilot study. Cold atmospheric plasma treatment or sham control treatment were applied respectively in randomly assigned and symmetric skin lesions. Three treatment sessions were performed at weeks 0, 1, and 2. Clinical severity indices were assessed at weeks 0, 1, 2, and 4 after treatment. Additionally, the microbial characteristics of the lesions before and after treatments were analyzed. We included 22 patients with mild to moderate atopic dermatitis presented with symmetric lesions. We found that cold atmospheric plasma can alleviate the clinical severity of atopic dermatitis. Modified atopic dermatitis antecubital severity and eczema area and severity index score were significantly decreased in the treated group. Furthermore, scoring of atopic dermatitis score and pruritic visual analog scales significantly improved. Microbiome analysis revealed significantly reduced proportion of Staphylococcus aureus in the treated group. Cold atmospheric plasma can significantly improve mild and moderate atopic dermatitis without safety issues.

Targeting Protective Catalase of Tumor Cells with Cold Atmospheric Plasma- Activated Medium (PAM)

2018 ◽  
Vol 18 (6) ◽  
pp. 784-804 ◽  
Author(s):  
Georg Bauer
Keyword(s):  
Control System ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Plasma Potential ◽  
Atmospheric Plasma ◽  
Antitumor Action ◽  
Active Principle ◽  
Reaction Products ◽  
Cold Atmospheric Plasma

Background: Application of cold atmospheric plasma to medium generates “plasma-activated medium” that induces apoptosis selectively in tumor cells and that has an antitumor effect in vivo. The underlying mechanisms are not well understood. Objective: Elucidation of potential chemical interactions within plasma-activated medium and of reactions of medium components with specific target structures of tumor cells should allow to define the active principle in plasma activated medium. Methods: Established knowledge of intercellular apoptosis-inducing reactive oxygen/nitrogen species-dependent signaling and its control by membrane-associated catalase and SOD was reviewed. Model experiments using extracellular singlet oxygen were analyzed with respect to catalase inactivation and their relevance for the antitumor action of cold atmospheric plasma. Potential interactions of this tumor cell-specific control system with components of plasma-activated medium or its reaction products were discussed within the scope of the reviewed signaling principles. Results: None of the long-lived species found in plasma-activated medium, such as nitrite and H2O2, nor OCl- or .NO seemed to have the potential to interfere with catalase-dependent control of apoptosis-inducing signaling of tumor cells when acting alone. However, the combination of H2O2 and nitrite might generate peroxynitrite. The protonation of peroxnitrite to peroxynitrous acid allows for the generation of hydroxyl radicals that react with H2O2, leading to the formation of hydroperoxide radicals. These allow for singlet oxygen generation and inactivation of membrane-associated catalase through an autoamplificatory mechanism, followed by intercellular apoptosis-inducing signaling. Conclusion: Nitrite and H2O2 in plasma-activated medium establish singlet oxygen-dependent interference selectively with the control system of tumor cells.

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