Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress

As spermatozoa are highly vulnerable to oxidative stress development, in vitro antioxidants offer an additional line of defense to the male reproductive system against oxidative insults. α‑tocopherol (α-TOC) is the most abundant form of vitamin E identified in the seminal plasma and spermatozoa membranes, being able to terminate numerous oxidative chain reactions causing substantial damage to biomolecules vital for sperm survival. This study was designed to shed more light on the in vitro effects of α‑tocopherol with respect to the vitality and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate-induced oxidative stress. Spermatozoa were washed out from 50 bovine ejaculates, suspended in 2.9 % sodium citrate and subjected to α-TOC treatment (10, 50, 100 and 500 μmol/L) in the presence or absence of ferrous ascorbate (FeAA; 150 μmol/L FeSO4 and 750 μmol/L ascorbic acid) during a 6h in vitro culture. Spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity (MTT) assay, and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the experiments in order to assess the intracellular activity of superoxide dismutase (SOD), catalase (CAT), as well as glutathione (GSH) and malondialdehyde (MDA) concentrations. Treatment with FeAA reduced both spermatozoa motility parameters (P < 0.001) as well as viability (P < 0.05 with respect to Time 0 h; P < 0.01 in case of Time 2 h and P < 0.001 in relation to Time 6 h), decreased the antioxidant parameters of the samples (P < 0.001 in case of SOD; P < 0.01 with respect to CAT and GSH) but increased the superoxide production (P < 0.01 in case of Time 0h and P < 0.001 with respect to Times 2 h and 6 h) and lipid peroxidation (P < 0.001). α‑TOC administration resulted in a preservation of the spermatozoa motility characteristics (P < 0.001 with respect to 500 μmol/L α-TOC), viability (P < 0.001 in case of 500 μmol/L α-TOC and P < 0.05 with respect to 100 μmol/L α-TOC) and antioxidant profile (P < 0.01 related to the impact of 500 μmol/L α-TOC on the SOD activity; P < 0.05 in relation to CAT; P < 0.01 with respect GSH; 100-500 μmol/L α-TOC), with 500 μmol/L α-TOC being the most effective. Our results suggest that α‑tocopherol possesses significant antioxidant properties that may prevent the deleterious effects caused by free radicals to spermatozoa, and extend the fertilization potential of male reproductive cells.

Download Full-text

Anti-Melanogenic Mechanism of Tetrahydrocurcumin and Enhancing Its Topical Delivery Efficacy Using a Lecithin-Based Nanoemulsion

Pharmaceutics ◽

10.3390/pharmaceutics13081185 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1185

Author(s):

Xudong Tang ◽

Qiaoru Dong ◽

Jun Li ◽

Fang Li ◽

Bozena B. Michniak-Kohn ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Model ◽

Antioxidant Properties ◽

Topical Delivery ◽

Related Protein ◽

B16f10 Melanoma ◽

Keratinocyte Cell ◽

The Impact ◽

Tyrosinase Related Protein

Tetrahydrocurcumin (THC) has been well known for its superior antioxidant properties. Therefore, it is speculated that it might be effective to relieve oxidative stress-induced diseases, such as skin hyperpigmentation. In this work, an in vitro B16F10 melanoma cell model was used to study the impact of THC on the melanogenic process under stressed conditions. It was demonstrated that THC could effectively inhibit the α-MSH (melanocyte-stimulating hormone) induced melanin production in B16F10 melanoma cells and the expressions of three key enzymes involved with the biosynthetic process of melanin, tyrosinase (TYR), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2), were all significantly reduced. In addition, an in vitro human keratinocyte cell model was used to investigate the potential protective role of THC on H2O2-induced cytotoxicity. It was found that THC could prevent H2O2-induced oxidative stress based on the results of both the cell viability study and the intracellular ROS (reactive oxygen species) study assessed by the flow cytometry. Last, THC was formulated into a lecithin based nanoemulsion, and an in vitro Franz diffusion cell study using Strat-M® membrane concluded that the nanoemulsion could significantly enhance the membrane permeation compared to the unformatted THC suspension. This research demonstrated the anti-melanogenic benefits of THC on the melanoma and keratinocyte cell models and the topical delivery efficacy could be significantly enhanced using a lecithin based nanoemulsion.

Download Full-text

Glucotoxicity Induced Oxidative Stress and InflammationIn VivoandIn VitroinPsammomys obesus: Involvement of Aqueous Extract ofBrassica rapa rapifera

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/3689208 ◽

2016 ◽

Vol 2016 ◽

pp. 1-14 ◽

Cited By ~ 3

Author(s):

Sihem Berdja ◽

Leila Smail ◽

Boualem Saka ◽

Samia Neggazi ◽

El-mehdi Haffaf ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Viability ◽

Brassica Rapa ◽

Histological Study ◽

Metabolic Parameters ◽

Sod Activity ◽

Oxidative Markers ◽

The Impact

Context.Brassica rapais considered as natural source of antioxidants and is used to treat diabetes.Objective. Our study carried the impact of glucotoxicity inducedin vivoandin vitroin vascular smooth muscle cells (VSMCs) inPsammomysand the therapeutic effect ofBrassica rapa(AEBr).Materials and Methods. We administered a hyperglucidic diet (30% sucrose) for 9 months and a treatment for 20 days with AEBr at 100 mg/kg. VSMCs were submitted to D-Glucose (0.6%) for 48 hours and treated with AEBr (2100 μg/mL) for 24 hours. We measured, in blood metabolic parameters, the redox statues and inflammatory markers in adipose tissue. Histological study was effectuated in liver. In VSMCs, we measured markers of glucotoxicity (IRS1p Serine, AKT) inflammation (NO, MCP1, TNFα, and NF-κB) and oxidative stress (oxidants and antioxydants markers). Cell viability and apoptosis were estimated by the morphological study.Results. AEBr corrects the metabolic parameters and inflammatory and oxidative markers in blood and homogenate tissue and reduces lipid droplets in liver. It induces, in VSMCs, a significant decrease of IRS1p serine, cyt c, NO, MCP1, TNFα, NF-κB, protein, and lipid oxidation and increases cell viability, AKT, ERK1/2, catalase, and SOD activity.Conclusion.Brassicaenhanced the antidiabetic, anti-inflammatory, and antioxidant defense leading to the protection of cardiovascular diseases.

Download Full-text

Selenium maintains cytosolic Ca2+ homeostasis and preserves germination rates of maize pollen under H2O2-induced oxidative stress

Scientific Reports ◽

10.1038/s41598-019-49760-3 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Alberto Marco Del Pino ◽

Marcello Guiducci ◽

Roberto D’Amato ◽

Alessandro Di Michele ◽

Giacomo Tosti ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Properties ◽

Close Correlation ◽

Sodium Selenate ◽

Protective Capacity ◽

Organic Form ◽

Maize Pollen ◽

Oxidative Challenge ◽

The Impact

Abstract Selenium (Se) displays antioxidant properties that can be exploited, in plants, to counteract abiotic stresses caused by overly-produced reactive oxygen species (ROS). Here, we show that fertigation of maize crops with sodium selenate effectively protects pollen against oxidative stress. Pollen isolated from Se-treated plants (Se1) and untreated controls (Se0) was incubated in vitro with H2O2 to produce oxidative challenge. Given the impact of ROS on Ca2+ homeostasis and Ca2+-dependent signaling, cytosolic Ca2+ was measured to monitor cellular perturbations. We found that H2O2 disrupted Ca2+ homeostasis in Se0 pollen only, while Se1 samples were preserved. The same trend was observed when Se0 samples were treated with sodium selenate or Se-methionine, which recapitulated in vitro the protective capacity of Se-fertigation. Furthermore, we found that germination rates were much better retained in Se1 as compared to Se0 (46% vs 8%, respectively) after exposure to 20 mM H2O2. The same was observed with Se0 pollen treated with Se-methionine, which is the organic form of Se into which most fertigated sodium selenate converts in the plant. These results, together, show a close correlation between ROS, Ca2+ homeostasis and pollen fertility, and provide strong evidence that Se-fertigation is an excellent approach to preserve or enhance agricultural productivity.

Download Full-text

Oxidative Stress Compromises Lymphocyte Function in Neonatal Dairy Calves

Antioxidants ◽

10.3390/antiox10020255 ◽

2021 ◽

Vol 10 (2) ◽

pp. 255

Author(s):

Wilmer Cuervo ◽

Lorraine M. Sordillo ◽

Angel Abuelo

Keyword(s):

Oxidative Stress ◽

Immune Responses ◽

Immune Cell ◽

Lymphocyte Activation ◽

Dairy Calves ◽

Lymphocyte Function ◽

Newborn Calves ◽

Ifn Γ ◽

The Impact

Dairy calves are unable to mount an effective immune response during their first weeks of life, which contributes to increased disease susceptibility during this period. Oxidative stress (OS) diminishes the immune cell capabilities of humans and adult cows, and dairy calves also experience OS during their first month of life. However, the impact that OS may have on neonatal calf immunity remains unexplored. Thus, we aimed to evaluate the impact of OS on newborn calf lymphocyte functions. For this, we conducted two experiments. First, we assessed the association of OS status throughout the first month of age and the circulating concentrations of the cytokines interferon-gamma (IFN-γ) and interleukin (IL) 4, as well as the expression of cytokine-encoding genes IFNG, IL2, IL4, and IL10 in peripheral mononuclear blood cells (PBMCs) of 12 calves. Subsequently, we isolated PBMCs from another 6 neonatal calves to investigate in vitro the effect of OS on immune responses in terms of activation of lymphocytes, cytokine expression, and antibody production following stimulation with phorbol 12-myristate 13-acetate or bovine herpesvirus-1. The results were compared statistically through mixed models. Calves exposed to high OS status in their first month of age showed higher concentrations of IL-4 and expression of IL4 and IL10 and lower concentrations of IFN-γ and expression of IFNG and IL2 than calves exposed to lower OS. In vitro, OS reduced lymphocyte activation, production of antibodies, and protein and gene expression of key cytokines. Collectively, our results demonstrate that OS can compromise some immune responses of newborn calves. Hence, further studies are needed to explore the mechanisms of how OS affects the different lymphocyte subsets and the potential of ameliorating OS in newborn calves as a strategy to augment the functional capacity of calf immune cells, as well as enhance calves’ resistance to infections.

Download Full-text

10-gingerol induces oxidative stress through HTR1A in cumulus cells: in-vitro and in-silico studies

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0042 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Kiptiyah Kiptiyah ◽

Widodo Widodo ◽

Gatot Ciptadi ◽

Aulanni’am Aulanni’Am ◽

Mohammad A. Widodo ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Culture ◽

Superoxide Dismutase ◽

In Silico ◽

Cumulus Cell ◽

Cumulus Cells ◽

Sod Activity ◽

In Silico Studies ◽

Incubation Periods

AbstractBackgroundWe investigated whether 10-gingerol is able to induce oxidative stress in cumulus cells.MethodsFor the in-vitro research, we used a cumulus cell culture in M199, containing 10-gingerol in various concentrations (0, 12, 16, and 20 µM), and detected oxidative stress through superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentrations, with incubation periods of 24, 48, 72, and 96 h. The obtained results were confirmed by in-silico studies.ResultsThe in-vitro data revealed that SOD activity and MDA concentration increased with increasing incubation periods: SOD activity at 0 µM (1.39 ± 0.24i), 12 µM (16.42 ± 0.35ab), 16 µM (17.28 ± 0.55ab), 20 µM (17.81 ± 0.12a), with a contribution of 71.1%. MDA concentration at 0 µM (17.82 ± 1.39 l), 12 µM (72.99 ± 0.31c), 16 µM (79.77 ± 4.19b), 20 µM (85.07 ± 2.57a), with a contribution of 73.1%. Based on this, the in-silico data uncovered that 10˗gingerol induces oxidative stress in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.Conclusions10-gingerol induces oxidative stress in cumulus cells through enhancing SOD activity and MDA concentration by inhibiting HTR1A functions and inactivating GSK3B and AKT˗1.

Download Full-text

Isolation and structural elucidation of dimeric epigallocatechin-3-gallate autoxidation products and their antioxidant capacity

European Food Research and Technology ◽

10.1007/s00217-021-03846-3 ◽

2021 ◽

Author(s):

Julian Alfke ◽

Uta Kampermann ◽

Svetlana Kalinina ◽

Melanie Esselen

Keyword(s):

Antioxidant Capacity ◽

Antioxidant Properties ◽

Cd Spectroscopy ◽

Trolox Equivalent Antioxidant Capacity ◽

Published Data ◽

Dietary Polyphenols ◽

Cellular Effects ◽

The Impact

AbstractDietary polyphenols like epigallocatechin-3-gallate (EGCG)—which represents the most abundant flavan-3-ol in green tea—are subject of several studies regarding their bioactivity and health-related properties. On many occasions, cell culture or in vitro experiments form the basis of published data. Although the stability of these compounds is observed to be low, many reported effects are directly related to the parent compounds whereas the impact of EGCG degradation and autoxidation products is not yet understood and merely studied. EGCG autoxidation products like its dimers theasinensin A and D, “P2” and oolongtheanin are yet to be characterized in the same extent as their parental polyphenol. However, to investigate the bioactivity of autoxidation products—which would minimize the discrepancy between in vitro and in vivo data—isolation and structure elucidation techniques are urgently needed. In this study, a new protocol to acquire the dimers theasinensin A and D as well as oolongtheanin is depicted, including a variety of spectroscopic and quadrupole time-of-flight high-resolution mass spectrometric (qTOF-HRMS) data to characterize and assign these isolates. Through nuclear magnetic resonance (NMR) spectroscopy, polarimetry, and especially circular dichroism (CD) spectroscopy after enzymatic hydrolysis the complementary atropisomeric stereochemistry of the isolated theasinensins is illuminated and elucidated. Lastly, a direct comparison between the isolated EGCG autoxidation products and the monomer itself is carried out regarding their antioxidant properties featuring Trolox equivalent antioxidant capacity (TEAC) values. These findings help to characterize these products regarding their cellular effects and—which is of special interest in the flavonoid group—their redox properties.

Download Full-text

Antioxidant properties of antiepileptic drugs levetiracetam and clonazepam in mice brain after in vitro-induced oxidative stress

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp2015.4358 ◽

2016 ◽

Vol 10 (14) ◽

pp. 278-288 ◽

Cited By ~ 2

Author(s):

de Albuquerque Oliveira Aline ◽

Isabel Linhares Maria ◽

Jos eacute Maia Chaves Filho Adriano ◽

Ricardo Vasconcelos Rios Emiliano ◽

Nayane de Carvalho Lima Camila ◽

...

Keyword(s):

Oxidative Stress ◽

Antiepileptic Drugs ◽

Antioxidant Properties ◽

Mice Brain

Download Full-text

Acrylamide-induced oxidative stress in human erythrocytes

Human & Experimental Toxicology ◽

10.1177/0960327109350664 ◽

2009 ◽

Vol 28 (10) ◽

pp. 611-617 ◽

Cited By ~ 30

Author(s):

Betul Catalgol ◽

Gül Özhan ◽

Buket Alpertunga

Keyword(s):

Oxidative Stress ◽

Reduced Glutathione ◽

Human Erythrocytes ◽

Antioxidant Enzyme Activities ◽

Total Glutathione ◽

Sod Activity ◽

Spectrophotometric Methods ◽

Low Concentrations ◽

Different Doses

Acrylamide (AA), a widely used industrial chemical, is shown to be neurotoxic, mutagenic and carcinogenic. This study was carried out to investigate the effects of different doses of AA on lipid peroxidation (LPO), haemolysis, methaemoglobin (MetHb) and antioxidant system in human erythrocytes in vitro. Erythrocyte solutions were incubated with 0.10, 0.25, 0.50 and 1.00 mM of AA at 37°C for 1 hour. At the end of the incubation, malondialdehyde (MDA), an end product of LPO, was determined by liquid chromatography (LC) while total glutathione, reduced glutathione (GSH) levels, activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzymes and the rates of haemolysis and MetHb were determined by spectrophotometric methods. All of the studied concentrations of AA increased MetHb formation and SOD activity, and induced MDA formation and haemolysis due to the destruction of erythrocyte cell membrane. AA caused a decrease in the activities of GSH-Px, CAT and GSH levels. However, these effects of AA were seen only at higher concentrations than AA intake estimated for populations in many countries. We suggest that LPO process may not be involved in the toxic effects of AA in low concentrations, although the present results showed that the studied concentrations of AA exert deteriorating effects on antioxidant enzyme activities, LPO process and haemolysis.

Download Full-text

Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress

Pharmaceutics ◽

10.3390/pharmaceutics10040212 ◽

2018 ◽

Vol 10 (4) ◽

pp. 212 ◽

Cited By ~ 7

Author(s):

Eliana Pintus ◽

Martin Kadlec ◽

Marija Jovičić ◽

Markéta Sedmíková ◽

José Ros-Santaella

Keyword(s):

Oxidative Stress ◽

Therapeutic Agent ◽

Antioxidant Properties ◽

In Vitro Effects ◽

Oxide Synthase ◽

Boar Spermatozoa ◽

Generating System ◽

Control Samples ◽

Inducible Nitric Oxide

Aminoguanidine is a selective inhibitor of the inducible nitric oxide synthase (iNOS) and a scavenger of reactive oxygen species (ROS). Numerous studies have shown the antioxidant properties of aminoguanidine in several cell lines, but the in vitro effects of this compound on spermatozoa under oxidative stress are unknown. In this study, we tested the hypothesis that aminoguanidine may protect against the detrimental effects of oxidative stress in boar spermatozoa. For this purpose, sperm samples were incubated with a ROS generating system (Fe2+/ascorbate) with or without aminoguanidine supplementation (10, 1, and 0.1 mM). Our results show that aminoguanidine has powerful antioxidant capacity and protects boar spermatozoa against the deleterious effects of oxidative stress. After 2 h and 3.5 h of sperm incubation, the samples treated with aminoguanidine showed a significant increase in sperm velocity, plasma membrane and acrosome integrity together with a reduced lipid peroxidation in comparison with control samples (p < 0.001). Interestingly, except for the levels of malondialdehyde, the samples treated with 1 mM aminoguanidine did not differ or showed better performance than control samples without Fe2+/ascorbate. The results from this study provide new insights into the application of aminoguanidine as an in vitro therapeutic agent against the detrimental effects of oxidative stress in semen samples.

Download Full-text

Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress

PeerJ ◽

10.7717/peerj.5136 ◽

2018 ◽

Vol 6 ◽

pp. e5136 ◽

Cited By ~ 8

Author(s):

Matheus Marcon ◽

Ricieri Mocelin ◽

Adrieli Sachett ◽

Anna M. Siebel ◽

Ana P. Herrmann ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Chronic Stress ◽

Brain Homogenate ◽

Enriched Environment ◽

Laboratory Animals ◽

Sod Activity ◽

Zebrafish Brain ◽

Positive Effects ◽

The Impact

Background The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Methods Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Results Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days increased CAT activity. Discussion Our findings reinforce the idea that EE exerts neuromodulatory effects in the zebrafish brain. EE promoted positive effects as it helped maintain the redox homeostasis, which may reduce the susceptibility to stress and its oxidative impact.

Download Full-text