scholarly journals Treatment with Linezolid or Vancomycin in Combination with Rifampin Is Effective in an Animal Model of Methicillin-ResistantStaphylococcus aureusForeign Body Osteomyelitis

2010 ◽  
Vol 55 (3) ◽  
pp. 1182-1186 ◽  
Author(s):  
Paschalis Vergidis ◽  
Mark S. Rouse ◽  
Gorane Euba ◽  
Melissa J. Karau ◽  
Suzannah M. Schmidt ◽  
...  
Keyword(s):  
Animal Model ◽  
Foreign Body ◽  
Proximal Tibia ◽  
Bacterial Load ◽  
Control Group ◽  
Treatment Groups ◽  
Titanium Wire ◽  
Experimental Rat Model ◽  
Rifampin Resistance ◽  
All Treatment

ABSTRACTRifampin monotherapy was compared to the combination of linezolid or vancomycin with rifampin in an experimental rat model of methicillin-resistantStaphylococcus aureus(MRSA) chronic foreign body osteomyelitis. MRSA was inoculated into the proximal tibia, and a titanium wire was implanted. Four weeks after infection, rats were treated intraperitoneally for 21 days with rifampin alone (n= 16), linezolid plus rifampin (n= 14), or vancomycin plus rifampin (n= 13). Thirteen animals received no treatment. At completion of treatment, qualitative cultures of the wire and quantitative cultures of the bone (reported as median values) were performed. Quantitative cultures from the control, rifampin monotherapy, linezolid-plus-rifampin, and vancomycin-plus-rifampin groups revealed 4.54, 0.71, 0.10, and 0.50 log10CFU/gram of bone, respectively. The bacterial load was significantly reduced in all treatment groups compared to that in the control group. Rifampin resistance was detected in isolates from 10, 2, and 1 animal in the rifampin, linezolid-plus-rifampin, and vancomycin-plus-rifampin groups, respectively. Cultures of the removed wire revealed bacterial growth in 1 and 2 animals in the rifampin and linezolid-plus-rifampin groups, respectively, with no growth in the vancomycin-plus-rifampin group and growth from all wires in the untreated group. In conclusion, we demonstrated that combination treatment with linezolid plus rifampin or vancomycin plus rifampin is effective in an animal model of MRSA foreign body osteomyelitis in the context of retention of the infected foreign body.

scholarly journals Activity of Omadacycline in Rat Methicillin-Resistant Staphylococcus aureus Osteomyelitis

2021 ◽  
Author(s):  
Melissa J. Karau ◽  
Suzannah M. Schmidt-Malan ◽  
Scott A. Cunningham ◽  
Jayawant N. Mandrekar ◽  
Bobbi S. Pritt ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Combination Therapy ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bacterial Load ◽  
Potential Treatment ◽  
Combination Therapies ◽  
Treatment Groups ◽  
Experimental Rat Model ◽  
Rifampin Resistance ◽  
All Treatment

Omadacycline, vancomycin and rifampin as well as rifampin combination therapies were evaluated in an experimental rat model of MRSA osteomyelitis. All treatment groups had less MRSA recovered than saline-treated animals. Emergence of rifampin resistance was observed in 3 of 16 animals with rifampin monotherapy, and none with rifampin combination therapy. After treatment, the median tibial bacterial load was 6.04, 0.1, 4.81 and 5.24 log 10 cfu/g for saline-, rifampin-, vancomycin- and omadacycline-treated animals. Omadacycline or vancomycin administered with rifampin yielded no detectable MRSA. Omadacycline, administered with rifampin, deserves evaluation in humans as a potential treatment for osteomyelitis.

357 CALCIUM RELEASE INDUCED BY THIMEROSAL AND INOSITOL 1,4,5-TRIPHOSPHATE IN HEAT-SHOCKED PORCINE OOCYTES

2007 ◽  
Vol 19 (1) ◽  
pp. 294
Author(s):  
J.-K. Tseng ◽  
P.-C. Tang ◽  
J.-C. Ju
Keyword(s):  
Heat Shock ◽  
Calcium Release ◽  
Prolonged Exposure ◽  
Imaging System ◽  
Developmental Competence ◽  
Control Group ◽  
Acetoxymethyl Ester ◽  
Treatment Groups ◽  
All Treatment

Elevated ambient temperature has been known to be deleterious to the developmental competence of mammalian oocytes and embryos, although the mechanism is still unclear. The objective of this study was to determine the effect of heat shock (HS) on the alteration of intracellular calcium concentrations ([Ca2+]i) of matured pig oocytes by two different calcium releasing agents. Porcine cumulus–oocyte complexes were aspirated from the follicles (3–6 mm) and subjected to standard in vitro maturation procedure for 42 h. Matured oocytes were then randomly allocated to different heat treatments at 41.5°C for 0 (Control, C0h), 1 (HS1h), 2 (HS2h), or 4 h (HS4h). An additional control group was cultured for 4 h without heat shock (C4h). Oocytes were incubated with 2 µM fura-2 acetoxymethyl ester (AM) and 0.02% pluronic F-127 in Ca2+-free PBS (40 min) following heat shock, and then washed with Ca2+-free PBS (30 min) for detection of [Ca2+]i. Fluorescent images were captured with alternative excitation wavelengths at 340/380 nm by a rotating chopper disk equipped with an Axon imaging system. Data from both experiments were analyzed by ANOVA using the General Linear Model (GLM) of the SAS (SAS Institute, Inc., Cary, NC, USA). In Experiment 1, matured oocytes were activated by 200 mM thimerosal (10 min) following heat treatment. The maximal [Ca2+]i in the HS2h group was the highest among all treatment groups. The lowest maximal peak of [Ca2+]i was observed in the HS4h group, but it was still higher than that in the C4h group (P < 0.05). The total amount of Ca2+ release represented by the total area of the peaks in C4h was lower than in any other groups except HS4h (P < 0.05). In Experiment 2, each matured oocyte was injected with approximately 10 pL of inositol 1,4,5-triphosphate (IP3, 0.5 mM); the Ca2+ transient was recorded as described in the previous experiment. The maximal value of [Ca2+]i in the C4h group was still the lowest among the heat-shocked and C0h groups (P < 0.05). The total Ca2+ release in the HS2h group was the highest among all treatment groups, but only significantly higher than the HS1h and C4h groups (P < 0.05). A similar pattern of Ca2+ release in HS-oocytes was induced by thimerosal and IP3 stimulations. These results indicate that Ca2+ releasing capacity of matured pig oocytes is enhanced by a shorter duration of heat shock, but declines after prolonged exposure of heat shock and/or in vitro culture. The differential Ca2+ releasing capacity of heat-shocked oocytes prior to fertilization revealed physiological changes of pig oocytes after heat shock. This finding provides further insight for the low fertilization and developmental competence that occurs in farm species during hot seasons.

scholarly journals The Electricidal Effect Is Active in an Experimental Model of Staphylococcus epidermidis Chronic Foreign Body Osteomyelitis

2009 ◽  
Vol 53 (10) ◽  
pp. 4064-4068 ◽  
Author(s):  
Jose L. Del Pozo ◽  
Mark S. Rouse ◽  
Gorane Euba ◽  
Cheol-In Kang ◽  
Jayawant N. Mandrekar ◽  
...  
Keyword(s):  
Foreign Body ◽  
Staphylococcus Epidermidis ◽  
Bacterial Load ◽  
Rabbit Model ◽  
Electrical Current ◽  
Bacterial Biofilms ◽  
Control Group ◽  
Doxycycline Treatment ◽  
Continuous Delivery

ABSTRACT Treatment with low-amperage (200 μA) electrical current was compared to intravenous doxycycline treatment or no treatment in a rabbit model of Staphylococcus epidermidis chronic foreign body osteomyelitis to determine if the electricidal effect is active in vivo. A stainless steel implant and 104 CFU of planktonic S. epidermidis were placed into the medullary cavity of the tibia. Four weeks later, rabbits were assigned to one of three groups with treatment administered for 21 days. The groups included those receiving no treatment (n = 10), intravenous doxycycline (n = 14; 8 mg/kg of body weight three times per day), and electrical current (n = 15; 200 μA continuous delivery). Following treatment, rabbits were sacrificed and the tibias quantitatively cultured. Bacterial load was significantly reduced in the doxycycline (median, 2.55 [range, 0.50 to 6.13] log10 CFU/g of bone) and electrical-current (median, 1.09 [range, 0.50 to 2.99] log10 CFU/g of bone) groups, compared to the level for the control group (median, 4.16 [range, 3.70 to 5.66] log10 CFU/g of bone) (P < 0.0001). Moreover, treatment with electrical current was statistically significantly more efficacious (P = 0.035) than doxycycline treatment. The electricidal effect (the bactericidal activity of low-amperage electrical current against bacterial biofilms) is active in vivo in the treatment of experimental S. epidermidis chronic foreign body osteomyelitis.

scholarly journals EFEKTIVITAS PENAMBAHAN VITAMIN C (ASCORBIC ACID) PADA PAKAN KOMERSIAL UNTUK PENGENDALIAN PENYAKIT KOI HERPESVIRUS (KHV) PADA IKAN MAS, Cyprinus carpio

2016 ◽  
Vol 5 (3) ◽  
pp. 425
Author(s):  
Taukhid Taukhid ◽  
Angela Mariana Lusiastuti
Keyword(s):  
Survival Rate ◽  
Vitamin C ◽  
Clinical Symptoms ◽  
Control Group ◽  
Commercial Diet ◽  
Treatment Groups ◽  
Koi Herpesvirus ◽  
B Vitamin ◽  
Different Doses ◽  
All Treatment

Riset dengan tujuan untuk mengevaluasi teknik pengendalian penyakit KHV pada ikan mas melalui penggunaan imunostimulan yaitu penambahan vitamin C pada pakan komersial dengan dosis yang berbeda telah dilakukan pada skala laboratorium. Perlakuan yang diterapkan adalah penambahan vitamin C jenis CFC-90 (microencapsulated vitamin C) pada pakan komersial dengan berbagai dosis yaitu: (A) tanpa vitamin C, sebagai kontrol, (B) vitamin C sebanyak 250 mg/kg pakan, (C) vitamin C sebanyak 500 mg/kg pakan, (D) vitamin C sebanyak 750 mg/kg pakan, dan (E) vitamin C sebanyak 1.000 mg/kg pakan. Masing-masing perlakuan dilakukan pengulangan sebanyak 3 (tiga) kali. Pada hari ke-15, seluruh kelompok perlakuan diinfeksi KHV secara buatan dengan teknik kohabitasi. Pengamatan dilakukan terhadap tingkah laku, gejala klinis, dan mortalitas ikan uji yang dilakukan setiap hari hingga akhir percobaan. Rataan persen sintasan ikan uji tertinggi pada akhir riset diperoleh pada penambahan vitamin C sebanyak 750 mg/kg pakan yaitu sebesar 82,22%; diikuti dengan penambahan vitamin C 250 mg/kg pakan sebesar 70,00%; vitamin C 1.000 mg/kg pakan sebesar 61,11%; vitamin C 500 mg/kg pakan sebesar 58,89%; sedangkan rataan sintasan terendah diperoleh pada kelompok kontrol yaitu sebesar 27,78%.Research with the aim to evaluate the technique of KHV disease control carp through the use of immunostimulatory namely the addition of vitamin C in commercial diet with different doses have been performed in a laboratorial scale. The treatment applied was the addition of vitamin C of CFC-90 (microencapsulated vitamin C) in the commercial diet with different doses, namely: (A) without vitamin C, as control, (B) Vitamin C 250 mg/kg feed, (C) vitamin C of 500 mg/kg feed, (D) Vitamin C 750 mg/kg feed, and (E) of vitamin C as much as 1,000 mg/kg feed. Each treatment was repeated three times. On day 15th, all treatment groups were artificially infected with KHV using cohabitation techniques. Observations conducted to monitor behavior, clinical symptoms and mortality of fish tests were done every day until the end of the experiment. The highest mean percent of survival rate was recorded in treatment D by adding vitamin C as much as 750 mg/kg of feed that was equal to 82.22%, followed by the addition of vitamin C 250 mg/kg diets at 70.00% (treatment B), Vitamin C 1,000 mg/kg feed amounted to 61.11% (treatment E), vitamin C 500 mg/kg diets at 58.89% (treatment C), while the lowest average of survival rate was obtained in the control group that was equal to 27.78%.

scholarly journals The Difference of Results in Abdomen and Anus Area Measurement With Non-Contact Infrared Thermometer

2021 ◽  
Vol 22 (2) ◽  
pp. 183-188
Author(s):  
Anna Surgean Veterini ◽  
Nancy Margarita Rehatta ◽  
Hamzah Hamzah ◽  
Widijiati Widijiati ◽  
Sarmanu Sarmanu ◽  
...  
Keyword(s):  
Animal Model ◽  
Body Temperature ◽  
Mean Difference ◽  
The Body ◽  
Area Measurement ◽  
Control Group ◽  
Control Groups ◽  
Infrared Thermometer ◽  
Treatment Groups ◽  
The Mean

Research about sepsis in animal model is interesting to get the right therapeutic method for human being. We did the research to study about the value of body temperature in mice’s various body area in order to get the important information if we are going to do the translational research in animal model about sepsis. One clinical signs of the sepsis mice model is changes in body temperature. An easy way to examine body temperature is using infrared non-contact thermometer. This study aimed to compare the body temperature using infrared non-contact thermometer at the abdomen and anal area. We used male mice, weighing 25–30 g, divided into two groups (control and treatment groups). The control group injected with NaCl 0.9% solution, with the amount of NaCl 0.9% volume equal to Lipopolysaccharide (LPS). In the treatment group injected with 2.5 mg/kg BW of LPS intraperitoneally. Body temperature was measured in abdomen (tabd) and ananl (tan) area at 8th and 24th hour after treatment. Body temperature value tabd was higher than tan. Lipopolysaccharide injection increase body temperature but was not significant when compared to the control group (8th and 24th hour). The mean difference between tabd and tan in 8th control groups were 2.12oC respectively. The mean difference between tabd and tan in 24th hour control groups 4.6oC. The mean difference in treatment groups (8th hour) was 4.66oC, while it was 4.77oC in the 24th groups. Giving 2.5 mg/kg BW LPS intraperitoneally did not rise the body temperature significantly as compare to control groups. But, body temperature at anus area using non-contact infrared thermometer after treatment showed lower results as compared to that of at abdomen significantly.

scholarly journals Salep Kullanımının Fermente Türk Sucuğu Kalite Parametreleri Üzerine Etkisi

2018 ◽  
Vol 6 (2) ◽  
pp. 219
Author(s):  
İsmail Gök ◽  
Birol Kılıç ◽  
Cem Okan Özer
Keyword(s):  
Shelf Life ◽  
Storage Period ◽  
Quality Parameters ◽  
Control Group ◽  
Meat Industry ◽  
Treatment Groups ◽  
Ph Values ◽  
Tbars Values ◽  
And Storage ◽  
All Treatment

The aim of this study, the effect of addition to salep at different concentrations (2.5, 5, 7.5 and 10%) into sucuk on physicochemical, textural and sensorial properties were investigated at d fermentation and storage periods (5., 10., 20. ve 30. days). The results indicated that TBARS values gradually increased during fermentation and storage periods in all treatment groups. However, addition of 10% salep decreased TBARS values compared to control group at the end of fermentation and storage period. pH values of sucuks were decreased with addition of salep. Higher L* values were observed in sucuk with 10% salep at the end of the fermentation and storage period. Increasing levels of salep in sucuk formulation caused an increase in hardness and ash values, a decrease in moisture values in sucuk. The results of this study indicated that salep may be used in sucuk manufacture by the meat industry to improve shelf-life and quality parameters of the product.

scholarly journals Inflammasome Regulation at the Level of Cellular and Molecular Function affect by Recombinant Tissue Plasminogen Activator and Exosomes Treatment in Ischemic Stroke Animal Model

2021 ◽  
Author(s):  
Mohsen Safakhil ◽  
Mina Ramezani ◽  
Azadeh Mohamadgholi
Keyword(s):  
Animal Model ◽  
Cell Death ◽  
Combination Therapy ◽  
Functional Recovery ◽  
Therapy Group ◽  
Neuronal Cell ◽  
Cresyl Violet ◽  
Treatment Groups ◽  
Cresyl Violet Staining ◽  
All Treatment

Abstract In the current research, neuroprotection and performance improvement have been investigated in MCAO animal model treated with exosomes combined with rt-PA. Middle cerebral artery occlusion (MCAO) was induced in 25 adult male Wistar rats. Rats received rt-PA with a dose of 100 µg/kg in a single dose or drived-exosome from bone marrow MSCs. The Garcia scoring system and elevated body test were employed as behavioral tests for the functional recovery assessment. Cresyl violet staining was applied to evaluate the cell death degree in brain tissues. Immunohistochemical analysis was performed for the detection of GFAP and IBA1-positive cells. Results Cresyl violet staining revealed that the population of dark cells was significantly reduced in all treatment groups. A considerable increase (P ≤ 0.05) in catalase enzyme was observed in the combination therapy group compared whit the MCAO group (P ≤ 0.05). The amount of GPX despite the increase in all treatment groups compared to MCAO group was not statistically significant (P ≥ 0.05). Our conclusion was approved by the Nlrp1 and Nlrp3 downregulation during combination therapy in MCAO model by reduction in cell death rate. The Density of GFAP-positive cells Showed a decrease in the exosome with or without rt-PA experimental groups in comparison with the MCAO group (P < 0.05). Our observation indicated that exosomes, in combination with rt-PA, resulted in the noticeable functional recovery, neuronal regeneration, and reduction of neuronal cell death after a 7-day period of the MCAO induction. This novel therapeutic strategy could provide a better treatment option for those patients suffered from stroke.

scholarly journals Gastric Histopathology of White Rats After Administration of Kedondong Leaves Extract

2019 ◽  
Vol 2 (1) ◽  
pp. 18
Author(s):  
I Wayan Sudira ◽  
I Made Merdana ◽  
Ketut Budiasa
Keyword(s):  
Inflammatory Cell ◽  
Ethanol Extract ◽  
Fatty Degeneration ◽  
Control Group ◽  
Histopathological Changes ◽  
Healthy Male ◽  
Treatment Groups ◽  
White Rats ◽  
Hematoxylin Eosin ◽  
All Treatment

This study aims to determine the effect of ethanol extract of Kedondong leaves (Spondias dulcis G. Forst) on the gastropathological histology of white rats (Mus musculus). A total of 25 healthy male white rats with weights 200-250 g were randomly divided into five groups. A control group was only given a placebo. The treatment group of kedondong leaf ethanol extract sequentially with a dose of 125, 250, 500 and 1,000 mg/kg/day for 21 days orally. On the 22nd day all of the animals were sacrificed for its the gastric, followed by processing for histopathological preparations with Hematoxylin Eosin (HE) staining. The results of the study in all treatment groups were found gastric histology changes in the form of mild inflammatory cell infiltration, mild fatty degeneration and mild necrosis. The number of mice that experienced histopathological changes differed in each group. It can be concluded that the oral administration of kedondong leaf ethanol extract with a range at a dose of 125-1000 mg/kg/d for 21 days does not cause significant histopathological structure disturbance in the stomach of male white rats.

Effects on puberty attainment in gilts of continuous or limited exposure to boars

1988 ◽  
Vol 28 (4) ◽  
pp. 469 ◽  
Author(s):  
PH Hemsworth ◽  
C Hansen ◽  
CG Winfield ◽  
JL Barnett
Keyword(s):  
Control Group ◽  
Early Puberty ◽  
Treatment Groups ◽  
Age At Puberty ◽  
Experimental Treatments ◽  
All Treatment

To identify alternative practical procedures for stimulating early puberty in gilts, 64 crossbred gilts were allotted to 1 of 4 treatments as follows: (1) 5-min daily boar exposure, (2) 15-min daily introduction to an area adjacent to 5 boars in their pens, (3) continuously housed with a mature vasectomised boar, or (4) control, no boar exposure. Experimental treatments commenced when the gilts averaged 176 days of age, and continued until oestrus was detected or the gilts reached an average age of 212 days. All treatment groups were significantly different from the control group for the following parameters; the percentage of giltsdetectedin oestrus (93.3, 68.8, 81.3 and 31.3%, respectively), the estimated age at puberty or first ovulation (189.0, 194.9, 188.0 and 203.4 days, respectively), and the percentage of gilts estimated to have attained puberty (100, 81.3, 100 and 43.8%, respectively). We conclude that daily introduction to a corridor adjacent to mature boars and continuous housing with a mature vasectomised boar are as effective in stimulating early puberty in gilts as is daily introduction to a mature boar, and may be more practical in several commercial situations.

Performance and blood chemistry in lambs following fasting and transport

1996 ◽  
Vol 62 (1) ◽  
pp. 49-56 ◽  
Author(s):  
G. M. J. Horton ◽  
J. A. Baldwin ◽  
S. M. Emanuele ◽  
J. E. Wohlt ◽  
L. R. McDowell
Keyword(s):  
Body Weight Gain ◽  
Live Weight ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Blood Parameters ◽  
Control Group ◽  
Plasma Urea ◽  
Treatment Groups ◽  
Control Food ◽  
All Treatment

AbstractThirty-six 5-month-old Dorset ram lambs (28·7 kg) were used to investigate the effects of fasting and transport on performance and selected blood parameters. Three treatment groups (no. = 12) were: (1) control-food and water, without transport; (2) fasting for 72 h, without transport; and (3) transport in a trailer to a nearby auction-barn, then driven 8 h/day for 3 days without food or water for a total of 72 h. Lambs were given a total mixed diet containing 163 g/kg crude protein throughout the 28-day post-transport period. Both fasted and transported lambs consumed less food than the control group during the first 7 days post treatment (P < 0·05). Water intake was similar for all treatment groups on the 1st day post transport, after which both fasted and transport lambs drank less ivater than control lambs during the following 6 days (P < 0·05). Live-weight loss after the 3-day fast and transport period and subsequent body-weight gain during the 28-day realimentation period differed across all treatment groups (P < 0·05 and P < 0·09; (1) 1% and 305 g, (2) 14·8% and 343 g, and (3) 20·0% and 390 g, respectively, though control lambs had the highest weight gains over the 32-day experimental period. Plasma urea nitrogen was lower in both fasted and transported lambs on days 6, 7 and 11 compared with control lambs (P < 0·05). Plasma glucose concentrations on days 4 and 5 were lower in transported lambs than in fasted lambs, and highest in control lambs (P < 0·05); glucose concentration levels remained lower in both fasted and transported lambs than in control lambs on days 6 and 7 (P < 0·05). Plasma cortisol concentrations were highest in transported lambs during the 3-day transport period and for 2 days immediately following transport (P < 0·05). Effects of the 72 h fast were exacerbated by auction barn activity and subsequent transport, and compensatory gains by fasted and transport lambs were incomplete within the 28-day post transport period.

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