scholarly journals Growth-Dependent Stable Carbon Isotope Fractionation by Basidiomycete Fungi: δ13C Pattern and Physiological Process

2002 ◽  
Vol 68 (10) ◽  
pp. 4956-4964 ◽  
Author(s):  
Matthew R. Henn ◽  
Gerd Gleixner ◽  
Ignacio H. Chapela
Keyword(s):  
Growth Medium ◽  
Fungal Biomass ◽  
Growth Stage ◽  
Stable Carbon Isotope ◽  
Axenic Culture ◽  
Growth Stages ◽  
Second Phase ◽  
C Isotopes ◽  
Low Concentrations ◽  
The Impact

ABSTRACT We grew 11 basidiomycetes in axenic culture to characterize their physiological capacities to fractionate stable C isotopes. Generally, δ13C values of the fungal biomass were (i) enriched in 13C relative to the growth medium, (ii) variable among the isolates, and (iii) dependent on the growth rate and growth stage of the fungi. We found a multiphasic dynamic of fractionation for Cryptoporus volvatus and Marasmius androsaceus during various growth stages. The first phase, P1, corresponded to the exponential growth stage and was characterized by an increasing enrichment in 13C content of the fungal biomass relative to the growth medium ranging between 4.6 and 6.9‰. The second phase, P2, exhibited a continual depletion in 13C of the fungal biomass, with the δ13C values of the fungal biomass asymptotically returning to the δ13C value of the growth medium at inoculation. The expression of the various fractionation phases was dependent on the amount of low-concentration micronutrients and growth factors added to the growth medium. The onset of P2 occurred at reduced concentrations of these elements. All of the sugars in the growth medium (sucrose, maltose, and glucose) were utilized for growth, indicating that the observed fractionation was not an artifact derived from the preferential use of 13C-rich maltose, which was found at low concentrations in the growth medium. In this study, we establish a framework with which to explore the impact of physiological fractionations by fungal interfaces on natural distributions of stable C isotopes.

scholarly journals Biological Control of the Weedy Plant (Rumex crispus) at the Seedling Growth Stage by the Green Dock Beetle (Gastrophysa viridula)

2019 ◽  
Vol 28 (1) ◽  
pp. 21-31
Author(s):  
Khalid S. Alshallash Khalid S. Alshallash
Keyword(s):  
Biological Control ◽  
Seedling Growth ◽  
Growth Stage ◽  
Biological Control Agent ◽  
Dry Weight ◽  
Control Agent ◽  
Growth Stages ◽  
Rumex Crispus ◽  
Shoot Number ◽  
The Impact

In four glasshouse experiments, the effectiveness of the adult green dock beetle Gastrophysa viridula (Coleoptera: Chrysomelidae), at the effective number of applied individuals, for use as a biological control agent of curled dock, Rumex crispus (Polygonaceae) were studied. The feeding of the beetle was investigated at four different numbers of beetle (0, 1, 2, 3) and at four seedling growth stages of the plant, defined by the average of leaf area per plant (1-1.22 , 2-4.45, 3-11.56, and 4-71.52 cm2/plant). Grazing by one, two or three dock beetles did not result in a significant reduction in dock dry weight or shoot numbers at the youngest growth stage. However, both at later seedling growth stages were significantly affected (P ? 0.0001), at any beetles number. The increase of beetle numbers caused nonsignificant increased effect, in some trials, confirming the impact of a single beetle. Three months after beetle grazing, dock seedlings of first, second and third growth stages were not able to regrow, however, some plants at the 4th growth stage, re-emerged. This suggested that the highest effect of beetle's feeding occurs on the early seedling stages. Statistical analysis showed a positive correlation (0.77) between dry weight and shoot number at all the four seedling growth stages, thus confirming the impact of the beetle on both the dry weight and shoot numbers. Combining beetle grazing with other control methods at older dock seedling stages could, therefore, provide better suppression

The effect of cotton growth stage on response to a sublethal concentration of 2,4-D

2019 ◽  
Vol 33 (2) ◽  
pp. 321-328 ◽  
Author(s):  
John T. Buol ◽  
Daniel B. Reynolds ◽  
Darrin M. Dodds ◽  
J. Anthony Mills ◽  
Robert L. Nichols ◽  
...  
Keyword(s):  
Fiber Length ◽  
Growth Stage ◽  
Field Experiments ◽  
Fruit Set ◽  
Sublethal Concentration ◽  
Growth Stages ◽  
Visible Injury ◽  
Fiber Properties ◽  
Low Concentrations ◽  
Auxin Herbicides

AbstractRecent commercialization of auxin herbicide–based weed control systems has led to increased off-target exposure of susceptible cotton cultivars to auxin herbicides. Off-target deposition of dilute concentrations of auxin herbicides can occur on cotton at any stage of growth. Field experiments were conducted at two locations in Mississippi from 2014 to 2016 to assess the response of cotton at various growth stages after exposure to a sublethal 2,4-D concentration of 8.3 g ae ha−1. Herbicide applications occurred weekly from 0 to 14 weeks after emergence (WAE). Cotton exposure to 2,4-D at 2 to 9 WAE resulted in up to 64% visible injury, whereas 2,4-D exposure 5 to 6 WAE resulted in machine-harvested yield reductions of 18% to 21%. Cotton maturity was delayed after exposure 2 to 10 WAE, and height was increased from exposure 6 to 9 WAE due to decreased fruit set after exposure. Total hand-harvested yield was reduced from 2,4-D exposure 3, 5 to 8, and 13 WAE. Growth stage at time of exposure influenced the distribution of yield by node and position. Yield on lower and inner fruiting sites generally decreased from exposure, and yield partitioned to vegetative or aborted positions and upper fruiting sites increased. Reductions in gin turnout, micronaire, fiber length, fiber-length uniformity, and fiber elongation were observed after exposure at certain growth stages, but the overall effects on fiber properties were small. These results indicate that cotton is most sensitive to low concentrations of 2,4-D during late vegetative and squaring growth stages.

Effects of Low-Dose Flumioxazin and Metribuzin Postemergence Applications on Soybean

2018 ◽  
Vol 33 (1) ◽  
pp. 87-94
Author(s):  
Daniel O. Stephenson ◽  
Todd A. Spivey ◽  
Michael A. Deliberto ◽  
David C. Blouin ◽  
Brandi C. Woolam ◽  
...  
Keyword(s):  
Growth Stage ◽  
Low Dose ◽  
Yield Loss ◽  
Economic Loss ◽  
Growth Stages ◽  
Post Injury ◽  
Width Reduction ◽  
The Impact

AbstractAll herbicides will move off-target to sensitive crops when not applied correctly. Therefore, low-dose applications of flumioxazin and metribuzin were evaluated in soybean at the unifoliate, V2, and V4 growth stages. Rates evaluated were 12.5%, 25%, and 50% of the labeled use rates of 72 and 316 g ai ha−1 of flumioxazin and metribuzin, respectively. Flumioxazin injury was characterized by necrosis and visible height and width reduction. Injury increased with rate 3 d after treatment (DAT), with unifoliate, V2, and V4 soybean injured 15% to 30%, 18% to 27%, and 5% to 8%, respectively. Unifoliate and V4 soybean were injured more than V4 soybean 3 to 14 DAT, but injury decreased to <5% by 42 DAT. Soybean yields in the flumioxazin study were 92% to 96% of the nontreated, resulting in a yield loss of 196 to 393 kg ha−1 and a revenue loss of 71 to 141 US$ ha−1. Metribuzin injury was primarily chlorosis with necrosis and a visible reduction in soybean height and width. Soybean at the V2 growth stage was injured 14% more than V4 soybean 3 DAT, regardless of metribuzin rate. Injury to V2 and V4 soybean was similar 14 DAT, with injury of 21% to 40% across rates. Soybean injury when treated at the V2 and V4 growth stages was 6% to 29% 42 DAT compared to unifoliate soybean at 0 to 17%. Soybean yields in the metribuzin study yields were 96% to 98% of the nontreated. However, a 2% to 4% reduction equates to a loss of 90 to 180 kg ha−1 and a revenue loss of 32 to 65 US$ ha−1. Unifoliate and V2 soybean are more sensitive to a low dose of flumioxazin POST, and V2 and V4 soybean are more sensitive to a low dose of metribuzin POST. Injury and the impact on soybean growth could potentially cause economic loss for a soybean producer.

scholarly journals 452 Stand Reduction and Foliage Damage Reduce Yield of Onion for Dehydration

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 471E-472
Author(s):  
George H. Clough
Keyword(s):  
Growth Stage ◽  
Leaf Growth ◽  
Field Trials ◽  
Growth Stages ◽  
Leaf Stage ◽  
Plant Growth Stages ◽  
The Impact ◽  
Stage Yield ◽  
Different Growth Stages ◽  
Production Characteristics

Field trials were conducted at Hermiston, Ore., from 1995 through 1998 to determine the impact of stand loss and plant damage at different growth stages on yield of onions grown for dehydration. The experiment was a complete factorial with four replications. Stand reduction (0%, 20%, 40%, 60%, 80%) and foliage damage (0%, 25%, 50%, 75%, or 100%) treatments were applied at 3-, 6-, 9-, and 12-leaf onion growth stages. All average onion production characteristics decreased linearly as stand reduction increased (plant population decreased) at all plant growth stages except average bulb weight which increased as stand was reduced. Bulb weight was not changed by up to 100% foliage removal at the three-leaf stage of growth. At the 6- and 12-leaf stages, bulb weight was reduced when >50% of the foliage was removed. The most severe response occurred at the nine-leaf stage when bulb weights were reduced the most. At the three-leaf stage, yield was not affected by foliage damage. At the six-leaf growth stage, yield was reduced by 75% or more foliage loss, but at the 9- and 12-leaf stages, >50% foliage removal reduced expected yields. As with bulb weight, the impact of foliage removal on yield was most severe at the nine-leaf growth stage.

Sensitive growth stages and temperature thresholds in wheat (Triticum aestivum L.) for index-based crop insurance in the Indo-Gangetic Plains of India

2015 ◽  
Vol 154 (2) ◽  
pp. 321-333 ◽  
Author(s):  
P. VIJAYA KUMAR ◽  
V. U. M. RAO ◽  
O. BHAVANI ◽  
A. P. DUBEY ◽  
C. B. SINGH ◽  
...  
Keyword(s):  
Heat Stress ◽  
Temperature Stress ◽  
Growth Stage ◽  
Crop Insurance ◽  
Critical Growth ◽  
Maximum Temperature ◽  
Growth Stages ◽  
Weather Index ◽  
Gangetic Plains ◽  
The Impact

SUMMARYHigh temperature stress at critical growth stages is a major risk factor for wheat in many wheat growing areas globally. Developing weather indices relating to yield reductions in wheat is an urgent requirement for weather-index-based crop insurance. The objectives of the present study were to: (i) identify critical phenological stage(s) for heat stress, (ii) quantify the impact of heat stress at critical growth stage(s) and (iii) work out thresholds of temperature for obtaining above average, average and below average yield of wheat. For achieving these objectives, 11 years’ experimental data for three cultivars (HD-2285, K-8804 and K-9107) under three sowing dates at the Kanpur Centre located in the Indo-Gangetic Plains of Uttar Pradesh, India were used. Among the eight phenological stages, the milk stage (growth stage 73) was identified as most sensitive for high maximum and minimum temperatures to adversely affect yield. The rate of yield reduction with unit increase in maximum and minimum temperatures (°C) was found to be highest in K-8804 and lowest in HD-2285. The optimum ranges of maximum temperature during anthesis, milk, dough and maturity stages are 19·7–21·9, 24·2–26·5, 26·1–28·8 and 29·5–30·8 °C, respectively and those for minimum temperature are 4·3–6·2, 8·3–9·7, 11·5–12·4 and 13·0–15·1 °C, respectively. The thresholds of temperature during critical stages and quantification of heat stress on yield will be of use in devising weather-index-based crop insurance products in wheat and also for breeding temperature-stress-resistant genotypes. This method of devising weather indices in the present study can be used in other crops and regions of the world as an adaptation strategy for climate change.

Herbicidal control of deathcamas (Zigadenus paniculatus)

2020 ◽  
pp. 1-5
Author(s):  
Clinton A. Stonecipher ◽  
Corey Ransom ◽  
Eric Thacker ◽  
Kevin Welch ◽  
Dale R. Gardner ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Stage ◽  
Control Agent ◽  
Alkaloid Content ◽  
Western United States ◽  
Growth Stages ◽  
Herbicide Application ◽  
The Mean ◽  
Similar Density ◽  
The Impact

Abstract Foothill deathcamas is a bulbous, perennial, native forb found throughout the western United States. Deathcamas begins growth early in the spring. The lack of alternative forages at this time can result in livestock becoming poisoned from the consumption of deathcamas. Research on herbicides for deathcamas control is limited to work from the 1950s and 1960s that identified 2,4-D as a control agent. The objective of this study was to evaluate alternative herbicide options for deathcamas control that include 2,4-D, 2,4-D + triclopyr, quinclorac, aminopyralid, imazapic, and chlorsulfuron. We also investigated the impact of plant growth stage on deathcamas control by making herbicide applications at two growth stages. One set of plots was treated with herbicides when deathcamas was in the early vegetative stage and the second set was treated at flowering. There is some evidence that stress might affect alkaloid content; therefore, we monitored alkaloid content of treated and nontreated deathcamas. Plots were established at Mt. Sterling, UT, and Mt. Pleasant, UT. Deathcamas density was reduced in 2,4-D, 2,4-D + triclopyr, and imazapic treatments 1 and 2 yr after herbicide application (P < 0.0001). Compared with the pretreatment densities, deathcamas densities(± standard error of the mean) 2 yr after herbicide application were reduced 96% ± 1.4%, 100% ± 0%, and 98% ± 0.9% for 2,4-D, 2,4-D + triclopyr, and imazapic, respectively, at the Mt. Sterling site. At the Mt. Pleasant site, deathcamas density was reduced by 84% ± 2.8% with 2,4-D alone, whereas 2,4-D + triclopyr and imazapic provided similar density reductions as observed at the Mt. Sterling site. Steroidal alkaloid concentrations did not change in herbicide-treated deathcamas at either stage of plant growth. These data indicate that 2,4-D, 2,4-D + triclopyr, and imazapic can effectively control deathcamas in the vegetative and flowering growth stages.

Greenhouse Data suggest that Growth Stage Impacts Phomopsis Stem Canker Severity Associated with Diaporthe gulyae on Sunflower (Helianthus annuus L.)

2021 ◽  
Author(s):  
Renan Guidini ◽  
Nathan Braun ◽  
Mammen Korah ◽  
Laura Marek ◽  
Febina Mathew
Keyword(s):  
Helianthus Annuus ◽  
Disease Severity ◽  
Growth Stage ◽  
Rating Scale ◽  
Stem Canker ◽  
Growth Stages ◽  
Post Inoculation ◽  
Resistance Response ◽  
Helianthus Annuus L ◽  
The Impact

Diaporthe gulyae Shivas et al. was initially described as causing Phomopsis stem canker of sunflower (Helianthus annuus L.) in the U.S. in 2010. In this study, the impact of growth stage on disease severity associated with D. gulyae in cultivated accessions was investigated. One hundred thirty-nine accessions were inoculated with a single isolate of D. gulyae using the mycelium contact inoculation method at V4-V6 (four to six true leaves) and R4-R6 (inflorescence to ray flower wilting) growth stages in the greenhouse. The experiment was conducted in a completely randomized design with six replications and repeated once. Disease severity was assessed at 14 days post-inoculation using a 0-to-5 rating scale. A significant interaction between growth stage and accessions (p<0.0001) was observed for disease severity [relative treatment effect (RTE)] caused by D. gulyae. Among the 139 accessions, 24, three, and two accessions had significantly lower RTE at V4-V6, R4-R6, and both growth stages respectively, based on 95% confidence intervals, when compared to the inbred susceptible check ‘HA 288’. This study suggests that accessions differ in their susceptibility to D. gulyae possibly because of changes in resistance response during host development. Future studies on sunflower-D. gulyae interaction are warranted for developing commercial hybrids with resistance to the causal fungus.

scholarly journals Variation of the Impact Aroma Compound, 2-Acetyl-1-Pyrroline, Content in Thai Fragrant Rice Plants and its Enhanced Accumulation by Soil Nutritional Elements

2020 ◽  
Vol 12 (6) ◽  
pp. 36
Author(s):  
Wichwara Nawara ◽  
Chonlada Bennett ◽  
Orranuch Norkaew ◽  
Phumon Sookwong ◽  
Sakul Moolkam ◽  
...  
Keyword(s):  
Growth Stage ◽  
Rice Cultivar ◽  
Mineral Elements ◽  
Aroma Compound ◽  
Growth Stages ◽  
Rice Varieties ◽  
Nutritional Elements ◽  
Flag Leaves ◽  
Jasmine Rice ◽  
The Impact

This study investigated the accumulation characteristics of 2-acetyl-1-pyrroline (2AP) in the Thai Jasmine rice cultivar, Khao Dawk Mali 105 (KDML 105) under different soil types and addition of soil nutritional elements. There was significant variation in the 2AP contents of rice leaves, not only among different growth stages, but also among different leaf positions at each growth stage. The highest 2AP content was seen in the third leaves at booting stage (25.10&plusmn;0.90 &micro;g g-1), whilst for flag leaves it was seen at mature grain stage (8.00&plusmn;0.72 &micro;g g-1). During the ripening stage, plants in sandy soil acquired higher 2AP content in flag leaves, spikelets, and seeds than in clay soil, with the highest 2AP content in flag leaves at 25.00&plusmn;0.70 &micro;g g-1. The accumulation of 2AP in basal, middle, and upper branches of panicles showed no significant differences, whereas a steady decrease in 2AP content was detected in the panicle axis and branches. The effects of individual mineral elements (B, Cr, Cu, Mg, Mn, and Se) on 2AP content was different at each growth stage. B enhanced the 2AP content by more than two-fold in the leaves at the heading stage (63.84&plusmn;4.56 &micro;g g-1) compared to that of the control set, followed by Mn, Cu, and Se. Similar to the above results, the 2AP content was also heightened in grains. The extended knowledge gathered in this study will assist farmers who wish to improve the fragrance qualities of their rice varieties.

scholarly journals Stand Reduction and Foliage Damage Reduce Yield of Dehydrating Onion

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 1005-1007 ◽  
Author(s):  
George H. Clough
Keyword(s):  
Plant Growth ◽  
Growth Stage ◽  
Leaf Growth ◽  
Field Trials ◽  
Growth Stages ◽  
Leaf Stage ◽  
Plant Growth Stages ◽  
The Impact ◽  
Stage Yield ◽  
Different Growth Stages

Field trials were conducted at Hermiston, Ore., from 1995 through 1998, to determine impact of stand loss and plant damage at different growth stages on yield of onions (Allium cepa) grown for dehydration. Stand reduction (0%, 20%, 40%, 60%, 80%) and foliage damage (0%, 25%, 50%, 75%, 100%) treatments were applied at three-, six-, nine-, and twelve-leaf onion growth stages. Although average bulb weight increased as stand was reduced, marketable, cull, and total yields decreased as stand reduction increased (plant population decreased) at all plant growth stages. Bulb weight was not changed by up to 100% foliage removal at the three-leaf stage. At the six- and twelve-leaf stages, weight was reduced when ≥50% of the foliage was removed. The most severe response occurred at the nine-leaf stage. At the three-leaf stage, yield was not affected by foliage damage. At the six-leaf growth stage, yield was reduced by 75% or more foliage loss, but at the nine- and twelve-leaf stages, ≥50% foliage removal reduced expected yields. As with bulb weight, the impact of foliage removal on yield was most severe at the nine-leaf growth stage.

The Action of an Aniline Derivative (AN 162) on Blood Coagulation and on Platelets

1971 ◽  
Vol 26 (01) ◽  
pp. 145-166
Author(s):  
E Deutsch ◽  
K Lechner ◽  
K Moser ◽  
L Stockinger
Keyword(s):  
Blood Coagulation ◽  
Citric Acid Cycle ◽  
Second Phase ◽  
Aniline Derivative ◽  
Acid Cycle ◽  
Enhancing Effect ◽  
Low Concentrations ◽  
Dual Action ◽  
High Concentrations ◽  
Induced Aggregation

Summary1. The aniline derivative AN 162, Donau Pharmazie, Linz, Austria, has a dual action on the blood coagulation: an anticoagulant and an coagulation enhancing effect.2. The anticoagulant action may only be demonstrated with high concentrations (over 1 X 10”3 M related to plasma) preferentially in PPP. It is partially caused by an inhibition of the endogenous way of generation of the prothrombin converting principle. In addition it is suggested that it interferes with the fibrinogen-fibrin reaction in a manner not yet understood.3. The coagulant action is caused by a greater availability of platelet constituents at low concentrations of AN 162 (over 1 × 10-4 M) and by the induction of a release reaction at higher concentrations. The platelet factors 3 and 4, serotonin, adenine, and acid phosphatase are released.4. AN 162 inhibits platelet aggregation. This inhibition can be demonstrated by the PAT of Breddin and in the stirred aggregation test of Born. It is more effective to inhibit the collagen-induced and the second phase of the adrenaline-induced aggregation than the ADP induced one. The platelet retention (test of Hellem) is also reduced.5. The action of AN 162 on the platelets is caused by a damage of the platelet membrane which becomes permeabel for both, soluble platelet constitutents and granula.6. AN 162 interferes with the energy metabolism of the platelets. It causes a loss of ATP, and inhibits the key-enzymes of glycolysis, citric acid cycle, fatty acid oxydation and glutathione reduction.7. AN 162 inhibits the growth of fibroblasts without influence on mitosis.

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