scholarly journals Dissemination of Fluoroquinolone-Resistant Campylobacter spp. within an Integrated Commercial Poultry Production System

2006 ◽  
Vol 72 (5) ◽  
pp. 3441-3447 ◽  
Author(s):  
Umelaalim Idris ◽  
Jingrang Lu ◽  
Marie Maier ◽  
Susan Sanchez ◽  
Charles L. Hofacre ◽  
...  

ABSTRACTWhile characterizing the intestinal bacterial community of broiler chickens, we detected ε-proteobacterial DNA in the ilea of 3-day-old commercial broiler chicks (J. Lu, U. Idris, B. Harmon, C. Hofacre, J. J. Maurer, and M. D. Lee, Appl. Environ. Microbiol.69:6816-6824, 2003). The sequences exhibited high levels of similarity toCampylobacter jejuniandCampylobacter colisequences, suggesting that chickens can carryCampylobacterat a very young age.Campylobactersp. was detected by PCR in all samples collected from the ilea of chicks that were 3 to 49 days old; however, it was detected only in the cecal contents of chickens that were at least 21 days old. In order to determine whether the presence ofCampylobacterDNA in young chicks was due to ingestion of the bacteria in food or water, we obtained commercial broiler hatching eggs, which were incubated in a research facility until the chicks hatched. DNA sequencing of the amplicons resulting fromCampylobacter-specific 16S PCR performed with the ileal, cecal, and yolk contents of the day-of-hatching chicks revealed thatCampylobacterDNA was present before the chicks consumed food or water. The 16S rRNA sequences exhibited 99% similarity toC. jejuniandC. colisequences and 95 to 98% similarity to sequences of other thermophilicCampylobacterspecies, such asC. lariandC. upsaliensis. The presence ofC. coliDNA was detected by specific PCR in the samples from chicks obtained from a commercial hatchery; however, noCampylobacterwas detected by culturing. In order to determine whether the same strains of bacteria were present in multiple levels of the integrator, we culturedCampylobactersp. from a flock of broiler breeders and their 6-week-old progeny that resided on a commercial broiler farm. The broiler breeders had been given fluoroquinolone antibiotics, and we sought to determine whether the same fluoroquinolone-resistant strain was present in their progeny. The isolates were typed by pulsed-field gel electrophoresis, which confirmed that the parental and progeny flocks contained the same strain of fluoroquinolone-resistantC. coli. These data indicate that resistantC. colican be present in multiple levels of an integrated poultry system and demonstrated that molecular techniques or more sensitive culture methods may be necessary to detect early colonization byCampylobacterin broiler chicks.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hussein M. Galal ◽  
M. I. Abdrabou ◽  
Ahmed H. I. Faraag ◽  
C. K. Mah ◽  
Azza M. Tawfek

AbstractThe broiler industry in the Middle East (ME) faces many challenges related to bacterial infections, including M. gallisepticum, M. synoviae, E. coli, and other gram-negative bacteria, exacerbated by various errors in the brooding process. Antibiotics use in the first three days of life, such as Linco-Spectin 100 SP, tilmicosin, enrofloxacin, tylosin, colistin, and doxycycline, is the trend in the market to control such challenges. This study aimed to evaluate the efficacy of the newly introduced aroA E. coli vaccine (Poulvac E. coli) and its ability to reduce over-reliance on the heavy use of antibiotics in the ME. The study was conducted on 160 broiler chicks, divided into eight even groups. Each group was treated differently in terms of antibiotic therapy and ages at the time of Poulvac E. coli administration and the challenge of virulent avian pathogenic E. coli (APEC), serotype O78. Spray application of Poulvac E. coli at seven days of age plus Linco-Spectin 100 SP during the first three days provided the best results for zero mortality after challenge with APEC, while Poulvac E. coli at seven days with enrofloxacin during the early three days resulted in 10% mortality. Poulvac E. coli hatchery vaccination protected birds against mortality but reduced body weight gain compared to the 7-day group vaccinated with Linco-Spectin 100 SP during the first three days. Poulvac E. coli given on day one or day seven did not affect the immune response to concurrent respiratory viral vaccines and, in some cases, improved response. This study shows that Poulvac E. coli at seven days of age, together with Linco-Spectin 100 during the first three days, has produced the best results in terms of protection and performance in the ME high presence of avian pathogenic E. coli field challenge.


Animals ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 162 ◽  
Author(s):  
Majid Shakeri ◽  
Jeremy Cottrell ◽  
Stuart Wilkinson ◽  
Mitchell Ringuet ◽  
John Furness ◽  
...  

Heat stress (HS) is an environmental stressor challenging poultry production and requires a strategy to cope with it. A total of 288-day-old male broiler chicks were fed with one of the following diets: basal diet, basal with betaine (BET), or with selenium and vitamin E (AOX), or with a combination of BET and AOX, under thermoneutral and cyclic HS. Results showed that HS reduced average daily feed intake (ADFI) (p = 0.01) and average daily gain (ADG) (p < 0.001), and impaired feed conversion ratio (FCR) (p = 0.03) during rearing period (0–42 day). BET increased ADG (p = 0.001) and decreased FCR (p = 0.02), whereas AOX had no effects. Breast muscle weight was decreased by HS (p < 0.001) and increased by BET (p < 0.001). Rectal temperature was increased by HS (p < 0.001) and improved by BET overall. Respiration rate was increased by HS (p < 0.001), but BET decreased it during HS (p = 0.04). Jejunum transepithelial resistance was reduced by HS and had no effect on permeability whereas BET increased jejunum permeability (p = 0.013). Overall, the reductions in ADG of broiler chickens during HS were ameliorated by supplementation with BET, with much of the increase in ADG being breast muscle.


2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Hesam Hosseinzadeh ◽  
Ali Ahmad Alaw Qotbi ◽  
Alireza Seidavi ◽  
David Norris ◽  
David Brown

The use of herbs and spices has gained increasing interest as feed additives and possible alternative to antibiotics in poultry production. The effects of using different levels of coriander seed powder or extract on selected blood parameters, intestinal microflora, and immune response of broiler chickens were investigated in this study. A total of 420-day-old broiler chicks were randomly assigned to 7 treatments with 4 replicates and fed for 42 days. Results showed that inclusion of 2.0% coriander powder in broiler diets lowered total cholesterol while blood urea was significantly higher in birds on T4compared to T1and T2. Furthermore, there were no treatment effects onLactobacillusbacteria; however, the population ofE. coliwas significantly higher in the ileum of chickens fed T0. Noticeable significant improvements of antibody titer against Newcastle, infectious bronchitis, and infectious bursal disease were observed in birds receiving coriander extract in water. Immunoglobulin G antibody against sheep red blood cells showed significant improvement in birds fed T3; likewise, immunoglobulin M was significantly higher in birds on T2and T3at 28 d of age. These results revealed that coriander extract or powder can be used as antibiotic alternative in broiler feeds.


2013 ◽  
Vol 80 (3) ◽  
pp. 943-950 ◽  
Author(s):  
Tyler E. Askelson ◽  
Ashley Campasino ◽  
Jason T. Lee ◽  
Tri Duong

ABSTRACTProbiotics have been demonstrated to promote growth, stimulate immune responses, and improve food safety of poultry. While widely used, their effectiveness is mixed, and the mechanisms through which they contribute to poultry production are not well understood. Microbial phytases are increasingly supplemented in feed to improve digestibility and reduce antinutritive effects of phytate. The microbial origin of these exogenous enzymes suggests a potentially important mechanism of probiotic functionality. We investigated phytate degradation as a novel probiotic mechanism using recombinantLactobacilluscultures expressingBacillus subtilisphytase.B. subtilis phyAwas codon optimized for expression inLactobacillusand cloned into the expression vector pTRK882. The resulting plasmid, pTD003, was transformed intoLactobacillus acidophilus,Lactobacillus gallinarum, andLactobacillus gasseri. SDS-PAGE revealed a protein in the culture supernatants ofLactobacilluspTD003 transformants with a molecular weight similar to that of theB. subtilisphytase. Expression ofB. subtilisphytase increased phytate degradation ofL. acidophilus,L. gasseri, andL. gallinarumapproximately 4-, 10-, and 18-fold over the background activity of empty-vector transformants, respectively. Phytase-expressingL. gallinarumandL. gasseriwere administered to broiler chicks fed a phosphorus-deficient diet. Phytase-expressingL. gasseriimproved weight gain of broiler chickens to a level comparable to that for chickens fed a control diet adequate in phosphorus, demonstrating proof of principle that administration of phytate-degrading probiotic cultures can improve performance of livestock animals. This will inform future studies investigating whether probiotic cultures are able to provide both the performance benefits of feed enzymes and the animal health and food safety benefits traditionally associated with probiotics.


2020 ◽  
Vol 47 (1) ◽  
pp. 221-233
Author(s):  
C. D. Tuleun ◽  
E. A. Okereke ◽  
T. A. Sunmola

Nutritional value of dietary raw bambara nut offal fortified with enzyme (Natuzyme ) on the performance of broiler chickens were investigated. Two hundred and fourty (240), unsexed one day-old commercial broiler chicks (Marshal Strain) were randomly divided into eight groups of 30 birds each. The groups were randomly assigned to eight iso-caloric and iso-nitrogenous diets in a 2 × 4 factorial arrangement involving four levels (0, 10, 20 and 30 %) of raw bambara nut offal and 2 enzyme levels (0 and 0.025 %). Each treatment was replicated three times with ten birds per replicate. There were significant differences (P < 0.05) among treatments in AFW, ADWG, ADFI, FCR and PER. Chickens fed 10 % BGO without ® Natuzyme had higher (P < 0.05) FBW, ADWG and ADFI than chickens fed 0, 20 and 30% BGO diets. Least (P<0.05) ADFI and better FCR was recorded on broiler chickens fed control diet and 20 % BGO with enzyme. respectively. Addition of enzyme improved (P < 0.05) AFW and ADWG at 20 and 30 % BGO level. Significantly (P<0.05) higher AFW, ADWG and ADFI revealed that inclusion level of BGO at 10 % did not pose any deleterious effects on the birds. There were significant (P < 0.05) differences among treatments in the digestibility of dry matter (DM), crude protein, crude fibre (CF), ether extract (EE) and nitrogen-free extract (NFE). There was no interaction (P>0.05) between enzyme and BGO across the dietary treatments. Significant (P < 0.05) differences were observed in all the blood profile parameters observed across the dietary treatments except eiosinophil concentration. It was concluded that up to 30 % BGO can be included in enzyme supplemented broiler finisher diet without adverse effects on the performance, nutrients digestibility and blood profile of broiler finisher chickens


2021 ◽  
Author(s):  
Olugbenga David Oloruntola

Abstract This study looked at the effects of processed kola nut husks meal (PKHM) utilization as a feed ingredient on broiler chicken in a 42-day feeding trial. Kola nut pod husks were processed into a PKHM using ash treatment and rumen liquor fermentation. Three experimental diets were developed at both the starter and finisher phases, with PKHM included at 0, 4, and 8%, and dubbed diets 1, 2, and 3, respectively. In a fully randomised design, 240 Arbor Acres broiler chicks were randomly assigned to three treatments (10 birds per replicate). Except for the significantly improved (P<0.05) feed conversion ratio of broiler chickens fed diets 2 and 3 at the grower phase (22-42 days) and overall (0-42 days), the performance indices were not significantly (P>0.05) affected by PKHM dietary inclusion. Broiler chicken carcass characteristics and relative internal organ weights remained constant (P>0.05) through diets. The serum glutathione concentration in broiler chickens fed an 8 percent PKHM inclusive diet increased significantly (P<0.05) than those on the control diet and 4 percent PKHM inclusive diet. When broiler chickens fed an 8 percent PKHM inclusive diet were compared to those fed a control diet, the serum catalase concentration was significantly higher (P<0.05). The total serum protein, creatinine, alanine aminotransferase, aspartate transferase, and cholesterol levels remained constant (P>0.05) regardless of dietary treatment. Dietary PKHM inclusion of up to 8% enhanced improved feed efficiency and increased antioxidant enzyme concentration and did not affect the serum biochemical indices concentration.Statement of NoveltyKola nut husk is one of the agro-wastes that contributes to environmental deterioration due to its underutilization. The kola nut husk is underutilised in broiler production due to its chemical constitution, minimal nutritional value, and poor digestion. In this research, kola nut husk meal was ash treated and rumen liquor fermented before being integrated at varying levels in the experimental diets and the performance characteristics, antioxidant status, serum protein, enzymes and biochemical were evaluated. The findings of this study could aid in the development of an agro-waste-based, low-cost functional feed for broiler chicks that incorporates the underutilised kola nut husk and other abundant agrowastes as macro-ingredients.


Author(s):  
A.O. Oso ◽  
R. Umaya Suganthi ◽  
P.K. Malik ◽  
G. Thirumalaisamy ◽  
V.B. Awachat

Background: The effect of a phyto-supplement (PS) composed of Aerva lanata, Piper betle, Cynodon dactylon and Piper nigrum on carcass characteristics was evaluated in commercial broiler chicken. Methods: A total of 192 day-old commercial broiler chicks were allocated to the four experimental groups and each group was separated into 6 replicates, with 8 birds per replicate. The dietary treatment groups were the positive control (basal diet + antibiotic, chlortetracycline @355g/ metric ton of feed), negative control (0% PS: basal diet only), 1% PS (basal diet +1% PS) and 2% PS (basal diet +2%PS). The trial lasted for 42 days (0 to 42 D of age). The carcass characteristics were recorded for two birds from each replicate at the end of the trial. Result: The broilers fed chlortetracycline and 1% PS recorded the highest (P=0.0205) and those fed only basal diet (negative control) recorded the lowest dressing percentage. Broilers fed diet supplemented with 1 and 2% PS exhibited higher (P less than 0.0001) weight and relative weight of lung than other treatments. Broilers fed diet supplemented with 1 and 2% PS exhibited lower weight (P=0.0032) and relative weight (P=0.0002) of liver when compared with the antibiotic treatment. Feeding 1% PS increased the relative weight of heart quadratically (P=0.044) while 2% PS reduced the weight of heart. Broilers on negative control diet recorded the highest weight (P=0.0002) and relative weight (P less than 0.0001) of kidney.


2020 ◽  
Vol 47 (3) ◽  
pp. 253-261
Author(s):  
Y. U. Kawu ◽  
A. I. Muhammad ◽  
H. Husa ◽  
U. D. Doma ◽  
M. Abubakar ◽  
...  

The consumer price for poultry products in Nigeria and the expansion of the commercial poultry industry are negatively affected by the utilization of competitive and costly ingredients in making poultry diets. Consequently, the identification and utilization of locally available alternatives would greatly reduce cost of poultry production and products. In this regard, a study was undertaken to examine the effects of replacement of yellow maize (YM) with gayamba pearl millet variety as dietary energy source on carcass yield and blood parameters of broiler chickens. Three hundred (300) Ross 308 broiler chicks weighing 167.94 – 173.07g were randomly assigned to four dietary treatments of 75 birds each. Each treatment was replicated thrice with 25 birds in a completely randomized design. The experimental diets contained 0, 33.33, 66.67 and 100% gayamba millet as replacement for YM designated as diets 1, 2, 3 and 4 respectively. Full-fat soya bean and groundnut cake meal were the main vegetable protein sources used in the diets. Feed and water were provided ad libitum and the experiment lasted for 49 days. Results for carcass yield indicated a significant (P<0.05) influence of diet on live weight (1828.33 – 2316.66 g), plucked weight (1622.83 – 2132.83 g), eviscerated weight (1429.50 – 1883.47 g) and carcass weight (1277.66 – 1680.33 g) in favour of diet 3. However, dressing percentage (69.00 – 72.50%) did not differ among treatments. In the same way, most of the relative weights of organs were also not significantly affected by diet. Except for the packed cell volume (29.81 – 35.04%), none of the haematological parameters showed significant difference among the treatments. Similarly, most of the serum biochemical indices apart from total proteins, albumin and globulin, did not differ significantly among treatments. It was therefore concluded that gayamba pearl millet can completely replace yellow maize in broiler chickens' diets with no adverse effect on carcass yield and blood constituents.


2021 ◽  
Vol 7 (1) ◽  
pp. 016-022
Author(s):  
Sami Ahmed Mohammad Arabi ◽  
Reem Adam Ibrahim Adam

Different unconventional feed stuffs are used in poultry production to decrease ration cost and the environmental, nutritional and economic benefits derived from the maximal utilization of animal blood. This study was established to assess the effect of feed partially replaced dried blood content supplemented on growth performance responses and carcass. In a feeding trial, 240 commercial broiler chicks a day old, with an average starting weight of 48.40± 1.50 g, Lehmann breed unsexed commercial broiler were randomly allocated to one of four dietary treatments in a completely randomized design. The dietary treatments consisted of the control diet, and three diets which contained varying levels of Sun-dried blood meals SDBM (3, 4 and 5 %) respectively. The experimental diets were formulated to be both isonitrgenous (22.5% CP) and isocaloric (3.10 Mcal /kg). Feed and water were provided adlibitum for a period of 6 weeks. Data for the entire 42 days growth assay indicate the concentration of SDBM in the diet had impact on feed intake. However, birds fed SDBM at 3 or 5% had better weight gains and feed efficiency (p < 0.05) than birds fed 4% SDBM and the SDBM free diet. Carcass yields were similar. Mortality was also unaffected by dietary treatments. In addition, Analysis of productive parameters indicated that dietary SDBM up to 5% had a positive effect on growth performance and that partial replacement of protein sources (blood meal and groundnut cake) with SDBM was possible.


2021 ◽  
Vol 48 (5) ◽  
pp. 152-164
Author(s):  
A. A. Akintomide ◽  
I. B. Osho ◽  
G. E. Onibi

The quest for sustainable poultry production using alternative feed ingredients is imperative. This study was conducted to assess the growth performance and blood profile of broiler chickens fed neem leaf meal (NLM) based diets with or without enzyme supplementation. Two hundred and eighty-eight, one-week old chicks (with an average weight of 98.44g) arranged in a completely randomized design with twelve birds/ replicate and three replicates/ treatment were fed NLM at 0, 1.5, 3 and 5% with or without Polyzme® to form eight experimental treatments. Data on initial body weights, weekly body weights and feed intake were taken during the trial which lasted for eight weeks and feed conversion ratio calculated. Blood was also collected for haematological studies at the 4 and 8 week by humanely severing the jugular vein. Results indicated that total weight gain at starter phase decreased progressively with increase in NLM inclusion and addition of Polyzme did not significantly (P>0.05) affect performance. At the finisher phase, growth performance was not significantly (P>0.05) influenced by NLM or enzyme addition. Furthermore, NLM with/without Polyzyme did not elicit any significant influence on the packed cell volume and haemoglobin concentration of the chickens. In conclusion, broiler chicks could tolerate up to 3% NLM and finishers up to 5% based on growth performance. The use of polyzyme at 400g/tonne in this study did not improve the utilization of NLM by the chickens.    La recherche de la production de volaille durable utilisant des ingrédients d'alimentation alternatifs est impérative. Cette étude a été réalisée pour évaluer la performance de la croissance et le profil sanguin des régimes à base de feuilles neem (NLM) avec ou sans supplémentation en enzyme. Deux cent quatre-vingt-huit poussins âgés d'une semaine (avec un poids moyen de 98,44 g) disposés dans un design complètement randomisé avec douze oiseaux / répliqués et trois répliqués / traitement ont été nourris à 0, 1,5, 3 et 5% avec ou sans Polyzme pour former huit traitements expérimentaux. Les données sur les poids corporels initiaux, les poids corporels hebdomadaires et l'apport alimentaire ont été pris au cours de l'essai qui a duré huit semaines et le ratio de conversion alimentaire calculé. Le sang a également été collecté pour des études hématologiques à la 4ème et 8ème semaine en coupant humainement la veine jugulaire. Les résultats ont indiqué que le gain de poids total à la phase de démarrage a diminué progressivement avec l'augmentation de l'inclusion de NLM et l'addition de polyzme n'a pas significativement (p> 0,05) affecter les performances. À la phase de finition, la performance de la croissance n'était pas significativement (p> 0,05) influencée par l'addition NLM ou enzyme. De plus, la NLM avec / sans polyzyme n'a suscité aucune influence significative sur le volume de la cellule emballé et la concentration en hémoglobine des poulets. En conclusion, les poussins de chair pourraient tolérer jusqu'à 3% de NLM et des finisseurs jusqu'à 5% sur la base de la performance de la croissance. L'utilisation de polyzyme à 400 g / tonne dans cette étude n'a pas amélioré l'utilisation de NLM par les poulets.


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