scholarly journals New method for concentration and quantitation of Mycobacterium leprae

1977 ◽  
Vol 5 (3) ◽  
pp. 326-328
Author(s):  
N Sato ◽  
A H Fieldsteel
Keyword(s):  
Pore Size ◽  
Standard Method ◽  
Membrane Filter ◽  
Mycobacterium Leprae ◽  
Glass Slide ◽  
New Method ◽  
Original Material ◽  
Polycarbonate Membrane ◽  
Standard Acid ◽  
Polycarbonate Filter

A new method of enumerating Mycobacterium leprae has been developed. Suspensions containing the organisms were filtered through a polycarbonate membrane filter (25-mm diameter, 0.4-micronm pore size, 10-micronm thick; Nucleopore) to concentrate the organisms. The membrane was then mounted on a glass slide and stained with a standard acid-fast stain. Finally, the membrane was treated with a small amount of chloroform to fix it to the slide and make it transparent. This method enabled us to detect M. leprae in quantities as small as 4.98 X 10(2) regardless of the total volume of the original material. Comparison with a standard method for enumerating M. leprae showed that both methods gave similar results when the organisms counted by the standard method were present in sufficient quantity for reproducibility. Because the least number of organisms that can be detected with the standard method is 10(4) ml and because the organisms detected with the new method could be concentrated on the polycarbonate filter from a large amount of infected fluid, a substantial number of suspensions were shown by the new method, but not by the standard method, to contain M. leprae.

Positronium Thermalization Process in Nanoporous Materials and its Influence on the Shape of PALS Spectrum

2008 ◽  
Vol 607 ◽  
pp. 39-41
Author(s):  
Jerzy Kansy ◽  
Radosław Zaleski
Keyword(s):  
Mesoporous Silica ◽  
Pore Size Distribution ◽  
Porous Materials ◽  
Pore Size ◽  
Size Distribution ◽  
Nanoporous Materials ◽  
New Method ◽  
New Model ◽  
Conventional Models ◽  
Mcm 41

A new method of analysis of PALS spectra of porous materials is proposed. The model considers both the thermalization process of positronium inside the pores and the pore size distribution. The new model is fitted to spectra of mesoporous silica MCM-41 and MSF. The resulting parameters are compared with parameters obtained from fitting the “conventional” models, i.e. a sum of exponential components with discrete or/and distributed lifetimes.

Tailoring the surface pore size of hollow fiber membranes in the TIPS process

2018 ◽  
Vol 6 (2) ◽  
pp. 535-547 ◽  
Author(s):  
Chuanjie Fang ◽  
Sungil Jeon ◽  
Saeid Rajabzadeh ◽  
Liang Cheng ◽  
Lifeng Fang ◽  
...  
Keyword(s):  
Pore Size ◽  
Hollow Fiber ◽  
Outer Layer ◽  
New Method ◽  
Hollow Fiber Membranes ◽  
Polymeric Solution ◽  
Fiber Membranes

A new method was used to tailor the surface pore size of PVDF hollow fiber membranes in the TIPS process by the co-extrusion of different solvents at the outer layer of the extruded polymeric solution.

Overnight Enumeration of Vibrio parahaemolyticus In Seafood by Hydrophobic Grid Membrane Filtration

1983 ◽  
Vol 46 (9) ◽  
pp. 783-786 ◽  
Author(s):  
PHYLLIS ENTIS ◽  
PETER BOLESZCZUK
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Membrane Filtration ◽  
Membrane Filter ◽  
New Method ◽  
Overnight Incubation ◽  
Differential Medium

A method was developed for direct enumeration of Vibrio parahaemolyticus in foods by hydrophobic grid membrane filter. The method consisted of a 4–5 h resuscitation step to recover injured cells, followed by overnight incubation at 42°C on V. parahaemolyticus Sucrose (VPS) agar, a new selective and differential medium. The confirmation rate of typical colonies on VPS agar was greater than 98%. The new method produced significantly higher counts of V. parahaemolyticus than the FDA method (P<0.01) when tested with chill-, freeze- or heat-stressed samples, and was equivalent to the FDA method (P>0.05) for recovery of osmotically stressed V. parahaemolyticus.

scholarly journals Complete Genome Sequence of Algoriphagus sp. Strain M8-2, Isolated from a Brackish Lake

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Yusuke Muraguchi ◽  
Koya Kushimoto ◽  
Yoshiyuki Ohtsubo ◽  
Tomohiro Suzuki ◽  
Hideo Dohra ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Pore Size ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Nucleotide Sequence ◽  
Complete Genome ◽  
Membrane Filter ◽  
Brackish Lake

Algoriphagus sp. strain M8-2 was isolated from a brackish lake, Lake Sanaru, in Hamamatsu, Japan, as a filterable bacterium through a 0.22-µm-pore-size membrane filter. We report here the complete nucleotide sequence of the M8-2 genome (a 3,882,610-bp chromosome).

Saccharose Particles as a Space Holder for Ti-Void Composite Preparation

2014 ◽  
Vol 894 ◽  
pp. 3-7 ◽  
Author(s):  
Grzegorz Adamek ◽  
Montasser Dewidar ◽  
Jarosław Jakubowicz
Keyword(s):  
Computed Tomography ◽  
Pore Size ◽  
Average Pore Size ◽  
New Method ◽  
Hard Tissue ◽  
Open Spaces ◽  
Average Pore ◽  
Metal Composites ◽  
Space Holder ◽  
Water Dissolution

In this work a new method of Ti-void composites (foam, scaffold) preparation is shown. In this process as a space holder particles we have applied a saccharose crystals (table sugar) with size up to 1.3 mm. After Ti and saccharose particles mixing and pressing, a green compacts composed of sugar and Ti grains were produced. Then, the sugar crystals were removed by its dissolution in water, which lead to open spaces (pores) formation in the green compacts. Then the compacts were sintered at 1250 °C. Alternatively, a sugar was evaporated during sintering without water dissolution. The foams were investigated by SEM, XRD and computed tomography. Such prepared void metal composites have porosity of about 72% and average pore size of about 0.7 mm. The pores have cubical shape corresponding to sugar crystals shape. The method is very promising in foams preparation and the made Ti-void composites can be applied for hard tissue implants, for example.

A Simple Reflectance Method for the Measurement of Particulate Pigment in Lake Water and its Application to Phosphorus–Chlorophyll–Seston Relationships

1980 ◽  
Vol 37 (1) ◽  
pp. 111-114 ◽  
Author(s):  
Martin Bergmann ◽  
Robert Henry Peters
Keyword(s):  
Total Phosphorus ◽  
Lake Water ◽  
Membrane Filter ◽  
New Method ◽  
Pigment Analysis ◽  
Membrane Filters ◽  
Total Pigment ◽  
Residual Variation

This paper describes a new method to measure total sestonic pigment based on the reduction in the amount of light reflected from a membrane filter after a given volume of lake water has passed through the filter. For a sample of 30 lakes, this index of "reflectance" gave a better regression on total phosphorus than did chlorophyll which suggests that part of the variation in published phosphorus–chlorophyll relationships results from the association of phosphorus with pigments other than chlorophyll. But the residual variation suggests both that the amount of pigment developed per unit of phosphorus varies among lakes and that the index does not completely represent the seston. Relationships between reflectance and concentration of chlorophyll, phosphorus, and seston are good enough to suggest that this rapid, easy analysis may find application as an adjunct to more traditional analyses whereever membrane filters are routinely used in the analysis of lake water.Key words: Total pigment analysis, seston, phosphorus–chlorophyll

A new method for preparation of an etched porous joint for capillary electrophoresis and its pore-size evaluation

2009 ◽  
Vol 30 (8) ◽  
pp. 1355-1361 ◽  
Author(s):  
Longhua Guo ◽  
Bin Qiu ◽  
Mingxing Chen ◽  
Guonan Chen
Keyword(s):  
Capillary Electrophoresis ◽  
Pore Size ◽  
New Method ◽  
Size Evaluation
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