scholarly journals Spatial variation of the colonic microbiota in patients with ulcerative colitis and control volunteers

Gut ◽  
2015 ◽  
Vol 64 (10) ◽  
pp. 1553-1561 ◽  
Author(s):  
A Lavelle ◽  
G Lennon ◽  
O O'Sullivan ◽  
N Docherty ◽  
A Balfe ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Spatial Variation ◽  
Colonic Microbiota ◽  
And Control

scholarly journals Fecal Microbiota Transplant in Two Ulcerative Colitis Pediatric Cases: Gut Microbiota and Clinical Course Correlations

2020 ◽  
Vol 8 (10) ◽  
pp. 1486
Author(s):  
Andrea Quagliariello ◽  
Federica Del Chierico ◽  
Sofia Reddel ◽  
Alessandra Russo ◽  
Andrea Onetti Muda ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Clinical Effects ◽  
Phylogenetic Distance ◽  
Fecal Microbiota Transplant ◽  
Inflammatory Bowel ◽  
Clinical Conditions ◽  
And Control

Fecal microbiota transplantation (FMT) is a promising strategy in the management of inflammatory bowel disease (IBD). The clinical effects of this practice are still largely unknown and unpredictable. In this study, two children affected by mild and moderate ulcerative colitis (UC), were pre- and post-FMT monitored for clinical conditions and gut bacterial ecology. Microbiota profiling relied on receipts’ time-point profiles, donors and control cohorts’ baseline descriptions. After FMT, the improvement of clinical conditions was recorded for both patients. After 12 months, the mild UC patient was in clinical remission, while the moderate UC patient, after 12 weeks, had a clinical worsening. Ecological analyses highlighted an increase in microbiota richness and phylogenetic distance after FMT. This increase was mainly due to Collinsella aerofaciens and Eubacterium biforme, inherited by respective donors. Moreover, a decrease of Proteus and Blautia producta, and the increment of Parabacteroides, Mogibacteriaceae, Bacteroides eggerthi, Bacteroides plebeius, Ruminococcus bromii, and BBacteroidesovatus were associated with remission of the patient’s condition. FMT results in a long-term response in mild UC, while in the moderate form there is probably need for multiple FMT administrations. FMT leads to a decrease in potential pathogens and an increase in microorganisms correlated to remission status.

scholarly journals Immune system alterations in patients with inflammatory bowel disease during remission

Medicina ◽  
2008 ◽  
Vol 44 (1) ◽  
pp. 27 ◽  
Author(s):  
Jurgita Šventoraitytė ◽  
Aida Žvirblienė ◽  
Gediminas Kiudelis ◽  
Rimantas Žalinkevičius ◽  
Aurelija Žvirblienė ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Bowel Disease ◽  
Immune Homeostasis ◽  
Th2 Cytokines ◽  
Inflammatory Bowel ◽  
Anti Inflammatory ◽  
And Control

Objective. Perturbed immune homeostasis elicited by misbalanced production of proinflammatory and anti-inflammatory cytokines is characteristic of inflammatory bowel disease. The aim of this study was to evaluate cytokine profile in patients with different forms of inflammatory bowel disease – ulcerative colitis and Crohn’s disease – during clinical remission phase. Material and methods. Production of proinflammatory Th1 cytokines (tumor necrosis factoralpha (TNF-a), interferon-gamma (IFN-g)) and anti-inflammatory Th2 cytokines (interleukin- 10 (IL-10) and interleukin-13 (IL-13)) was analyzed in peripheral blood mononuclear cells of patients with inflammatory bowel disease (9 with ulcerative colitis and 9 with Crohn’s disease) and control subjects (n=11) by enzyme-linked immunosorbent assay (two-site ELISA). Results. The results of the study revealed that the level of TNF-a after stimulation with phytohemagglutinin in patients with Crohn’s disease was significantly higher in comparison to both patients with ulcerative colitis and controls (P<0.001 and P<0.01, respectively). The secretion of IFN-g both in patients with Crohn’s disease and ulcerative colitis was lower than that in controls (P=0.05 and P<0.01, respectively), but it normalized after stimulation with phytohemagglutinin. The levels of IL-10 and IL-13 were significantly (P<0.01) higher in patients with Crohn’s disease than in patients with ulcerative colitis and control group before and after stimulation with phytohemagglutinin. Conclusions. The results of our study provide evidence that in patients with inflammatory bowel disease, the imbalance between production of proinflammatory Th1 and anti-inflammatory Th2 cytokines persists even during remission of the disease, and disturbances of immune homeostasis are significantly more expressed in patients with Crohn’s disease than in patients with ulcerative colitis.

scholarly journals P187 The significance of netrin-1 level in the clinical activity of ulcerative colitis, its association between TNF-α and IL-6

2020 ◽  
Vol 14 (Supplement_1) ◽  
pp. S232-S232
Author(s):  
H Korkmaz ◽  
K Fidan
Keyword(s):  
Ulcerative Colitis ◽  
Proinflammatory Cytokines ◽  
Activity Index ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Clinical Activity ◽  
Tnf Α ◽  
Significant Difference ◽  
And Control ◽  
Control Study

Abstract Background In this study, we investigated the importance of netrin-1 levels in ulcerative colitis (UC) in clinical activity of the disease, and its association with other proinflammatory cytokines IL-6 and TNF-α. Methods This study is a type of case–control study. Sixty-seven patients with UC (36 of them activation, 31 of remission) and 50 healthy controls were included in the study. UC patients; ‘Truelove Witts clinical activity index by remission (n = 31), mild activation (n = 21), moderate activation (n = 6) and severe activation (n = 9) were divided into groups. Netrin, IL-6 and TNF-α measurements in plasma samples were performed using enzyme-linked immunosorbent assay kit. Results Between the patient group and the control group; there was a statistically significant difference between netrin-1, IL-6, TNF-α, neutrophil, platelet (p &lt; 0.05 for all). The plasma netrin-1 mean of UC with severe activation group (139.21 ± 48.09 pg/ml) was statistically significantly higher than that of the mild activation (p = 0,037), remission group (p = 0,001) and control group(p = 0,011). The plasma netrin-1 mean of UC with moderate activation group was statistically significantly higher than that of the mild activation(p = 0,045) and remission group(p = 0,004). Conclusion Our results reveal that plasma netrin-1 levels have been shown to be associated with UC activation, similar to proinflammatory cytokines such as TNF-α and IL-6, in UC.

High-Definition Metrology Based Spatial Variation Pattern Analysis for Machining Process Monitoring and Diagnosis

2009 ◽  
Author(s):  
Hui Wang ◽  
Saumuy Suriano ◽  
Liang Zhou ◽  
S. Jack Hu
Keyword(s):  
Spatial Variation ◽  
Process Monitoring ◽  
Spatial Data ◽  
Control Charts ◽  
Pattern Analysis ◽  
Surface Region ◽  
High Definition ◽  
Statistical Process ◽  
Surface Variation ◽  
And Control

Non-contact high-definition measurement technology, such as laser holographic interferometry, makes it feasible to quickly inspect dimensional variation at micron level, providing up to 2 million data points over a surface area of up to 300×300 mm2. Such high-definition metrology (HDM) data contain rich spatial variation information but it is challenging to utilize this information for process monitoring and control. The spatial distribution of the data is in high-dimensional form and may show nonlinear patterns. Conventional statistical process monitoring and diagnostic schemes based on simple test statistics and linear statistical process models are incapable of capturing the complex surface characteristics as reflected by large amounts of spatial data. This paper develops a framework for efficient monitoring of spatial variation in HDM data using principal curves and quality control charts. Since large scale surface variation patterns (caused by fixturing and part bending) may camouflage those in the smaller scale (generally associated with tooling conditions), it is essential to separate the patterns in these scales and monitor them individually. At each scale, process monitoring is implemented in a sequential manner by monitoring the overall spatial features followed by localized variation identification if an out-of-control condition is detected. To examine the overall spatial characteristics, a principal-component-analysis (PCA) filtered principal curve regression is proposed in conjunction with multivariate control charts whereby nonlinear patterns of spatial data are extracted and monitored. When the overall monitoring indicates a problem, the identification of a surface variation change can be achieved through localized monitoring over each surface region based on variogram pattern analysis and control charts. The location of surface region change provides clues for variation source diagnosis. The proposed method is illustrated using simulated HDM data.

scholarly journals Construction of a Model Culture System of Human Colonic Microbiota to Detect Decreased Lachnospiraceae Abundance and Butyrogenesis in the Feces of Ulcerative Colitis Patients

2019 ◽  
Vol 14 (5) ◽  
pp. 1800555 ◽  
Author(s):  
Kengo Sasaki ◽  
Jun Inoue ◽  
Daisuke Sasaki ◽  
Namiko Hoshi ◽  
Tomokazu Shirai ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Culture System ◽  
Colonic Microbiota

scholarly journals A long noncoding RNA signature for ulcerative colitis identifies IFNG-AS1 as an enhancer of inflammation

2016 ◽  
Vol 311 (3) ◽  
pp. G446-G457 ◽  
Author(s):  
David Padua ◽  
Swapna Mahurkar-Joshi ◽  
Ivy Ka Man Law ◽  
Christos Polytarchou ◽  
John P. Vu ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
T Cells ◽  
Noncoding Rna ◽  
Inflammatory Cytokine ◽  
Inflammatory Responses ◽  
Cell Model ◽  
Differentially Expressed ◽  
P Value ◽  
Susceptibility Loci ◽  
And Control

High-throughput technologies revealed new categories of genes, including the long noncoding RNAs (lncRNAs), involved in the pathogenesis of human disease; however, the role of lncRNAs in the ulcerative colitis (UC) has not been evaluated. Gene expression profiling was used to develop lncRNA signatures in UC samples. Jurkat T cells were activated by PMA/ionomycin subsequently interferon-γ (IFNG) and tumor necrosis factor (TNF)-α protein levels were assessed by ELISA. Anti-sense molecules were designed to block IFNG-AS1 expression. A unique set of lncRNAs was differentially expressed between UC and control samples. Of these, IFNG-AS1 was among the highest statistically significant lncRNAs (fold change: 5.27, P value: 7.07E−06). Bioinformatic analysis showed that IFNG-AS1 was associated with the IBD susceptibility loci SNP rs7134599 and its genomic location is adjacent to the inflammatory cytokine IFNG. In mouse models of colitis, active colitis samples had increased colonic expression of this lncRNA. Utilizing the Jurkat T cell model, we found IFNG-AS1 to positively regulate IFNG expression. Novel lncRNA signatures differentiate UC patients with active disease, patients in remission, and control subjects. A subset of these lncRNAs was found to be associated with the clinically validated IBD susceptibility loci. IFNG-AS1 was one of these differentially expressed lncRNAs in UC patients and found to regulate the key inflammatory cytokine, IFNG, in CD4 T cells. Taking these findings together, our study revealed novel lncRNA signatures deregulated in UC and identified IFNG-AS1 as a novel regulator of IFNG inflammatory responses, suggesting the potential importance of noncoding RNA mechanisms on regulation of inflammatory bowel disease-related inflammatory responses.

scholarly journals The Effects of Flaxseed Supplementation On Circulating Adipokines Concentration in Patients With Ulcerative Colitis

2021 ◽  
Author(s):  
Nava Morshedzadeh ◽  
Mehran Rahimlou ◽  
Shabnam Shahrokh ◽  
Soheila Karimi ◽  
Vahid Chaleshi ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Controlled Trial ◽  
Final Analysis ◽  
Gastrointestinal Diseases ◽  
Beneficial Effects ◽  
Randomized Controlled ◽  
Significant Difference ◽  
Baseline Weight ◽  
Inflammatory Bowel ◽  
And Control

Abstract Introduction: Inflammatory bowel disease (IBD) is one of the most common gastrointestinal diseases that can affect people of all ages. Adipokines secreted from adipose tissue have been shown to play an important role in the pathogenesis of ulcerative colitis (UC). The aim of this study was to evaluate the effect of supplementation with your seed on the concentrations of adiponectin, resistin and visfatin in patients with UC.Methods: This trial is an open-labeled randomized controlled trial which conducted among 70 patients with UC. Patients were randomly divided into two groups: flaxseed and control. Patients in the intervention were received 30 g/day flaxseed powder for 12 weeks. Anthropometric, nutritional and biochemical factors of patients were evaluated at the beginning and end of the intervention period.Results: Totally, 64 patients (36 men and 28 women) with mean age of 31.12 ± 9.67 included in the final analysis. There wasn’t any significant difference between two groups in term of baseline weight and height (P>0.05). After the 12 weeks' intervention, flaxseed supplementation led to a significant reduction in the resistin (-4.85 ± 1.89 vs. -1.10 ± 2.25, P<0.001) and visfatin concentration (-1.33± 1.14 vs. -0.53 ± 1.63, P=0.018). Moreover, we found a significant increase in the adiponectin levels after the flaxseed supplementation (3.49 ± 1.29 vs. -0.35 ± 0.96, P<0.001).Conclusion: It has been reported in this study that flaxseed supplementation could exert beneficial effects on adipokine levels in patients with UC. Trial registration: IRCT registration no. IRCT20180311039043N1

scholarly journals Expression of Oncogenic Molecules in Pediatric Ulcerative Colitis

Digestion ◽  
2021 ◽  
pp. 1-9
Author(s):  
Nobuyasu Arai ◽  
Takahiro Kudo ◽  
Kazuhide Tokita ◽  
Reiko Kyodo ◽  
Masamichi Sato ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Ulcerative Colitis ◽  
Real Time ◽  
Real Time Pcr ◽  
Immunohistochemical Staining ◽  
Active Phase ◽  
Control Groups ◽  
Expression Levels ◽  
Remission Phase ◽  
And Control

<b><i>Introduction:</i></b> Long-term disease duration of ulcerative colitis (UC) is known to increase the risk of developing colorectal cancer in adults; however, this association has not been genetically analyzed in children with UC. Herein, we examined the expression of cancer-related genes in the colonic mucosa of pediatric UC patients and their risk of developing colorectal cancer. <b><i>Methods:</i></b> Microarray analysis of cancer-related gene expression was conducted on rectal mucosa biopsy specimens randomly selected from pediatric cases, including 4 active-phase UC cases, 3 remission-phase UC cases, and 3 irritable bowel syndrome control cases. The subject pool was then expanded to 10 active-phase cases, 10 remission-phase cases, and 10 controls, which were analyzed by real-time polymerase chain reaction (PCR) and immunohistochemical staining. <b><i>Results:</i></b> The microarray results indicated significantly higher expression levels of cancer-related genes <i>PIM2</i> and <i>SPI1</i> in the active group than in the remission and control groups (<i>p</i> &#x3c; 0.05). Real-time PCR confirmed that <i>PIM2</i> and <i>SPI1</i> expression levels were significantly higher, whereas <i>TP53</i> and <i>APC</i> expression levels were significantly lower, in the active-phase group than in the remission and control groups (<i>p</i> &#x3c; 0.05). Immunohistochemical staining for PIM2, SPI1, TP53, and APC proteins supported the real-time PCR results. <b><i>Conclusions:</i></b> Expression levels of previously unreported cancer-related genes in adult UC patients were significantly higher in pediatric UC patients than in controls. Inflammation of the gastrointestinal mucosa increased the expression levels of cancer-related genes even in childhood-onset UC cases, suggesting that chronic inflammation from childhood may increase the risk of colorectal cancer development.

Front Cover: Metaproteomics of Colonic Microbiota Unveils Discrete Protein Functions among Colitic Mice and Control Groups

PROTEOMICS ◽  
2018 ◽  
Vol 18 (3-4) ◽  
pp. 1870031
Author(s):  
Clara Moon ◽  
Gregory S. Stupp ◽  
Andrew I. Su ◽  
Dennis W. Wolan
Keyword(s):  
Control Groups ◽  
Front Cover ◽  
Colonic Microbiota ◽  
Protein Functions ◽  
And Control
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