Treponema pallidum PCR testing for diagnosis of mucocutaneous ulcers suspicious for syphilis

2021 ◽  
pp. sextrans-2021-055192
Author(s):  
Muhammad Hyder Junejo ◽  
Mark Collery ◽  
Gary Whitlock ◽  
Alan McOwan ◽  
Victoria Tittle ◽  
...  
Keyword(s):  
Demographic Data ◽  
Treponema Pallidum ◽  
Added Value ◽  
Contact Tracing ◽  
Bisexual Men ◽  
Electronic Patient Records ◽  
Serological Tests ◽  
Syphilis Infection ◽  
Cross Sectional ◽  
History Of

BackgroundPrimary syphilis is characterised by the appearance of an ulcerated lesion (chancre) on the anogenital or oral mucosa from which Treponema pallidum DNA may be detectable by PCR. Serological tests for syphilis may be non-reactive in early infection, even after the appearance of a chancre. We reviewed the use of a multiplex-PCR (M-PCR) test to determine the added value of T. pallidum DNA detection in the management of individuals presenting with mucocutaneous ulceration at a sexual health service in central London.MethodsWe performed a cross-sectional analysis of all individuals with detectable T. pallidum DNA from September 2019 to April 2020. Electronic patient records were reviewed and concomitant results for treponemal serology and/or rapid plasma reagin (RPR) extracted, along with demographic data, history of syphilis and indices of sexual behaviour including number of sexual partners contacted. Any subsequent treponemal serology and RPR results were also reviewed.ResultsM-PCR swab specimens were performed in 450 individuals, of whom 63 (14%) had detectable T. pallidum DNA; 60 of 63 (95%) were gay or bisexual men and 11 of 63 (17%) were living with HIV. A history of treated syphilis was present in 17 of 63 (27%). Same-day treponemal serology/RPR testing was performed in 58 of 63 (92%) patients. Of the 58 who had same-day syphilis serology/RPR, 9 (16%) had their syphilis infection confirmed by treponemal DNA PCR alone. A total of 165 partners were traced as contacts of infection, of whom 25 (15%) were contacts of individuals diagnosed by M-PCR testing alone.ConclusionIn individuals with T. pallidum PCR-positive lesions, around one in six in our cohort were negative on standard diagnostic serological tests for syphilis. Treponemal DNA testing is an important addition to serological assays in individuals with mucocutaneous ulceration who are at risk of recent syphilis infection and facilitates early diagnosis and contact tracing.

Treponemal infection rates, risk factors and pregnancy outcome in a rural area of Senegal

2003 ◽  
Vol 14 (3) ◽  
pp. 208-215 ◽  
Author(s):  
Emmanuel Lagarde ◽  
Emmanuelle Guyavarch ◽  
Jean-Pierre Piau ◽  
AÏssatou Gueye-Ndiaye ◽  
Karim Seck ◽  
...  
Keyword(s):  
Treponema Pallidum ◽  
Population Based ◽  
Cross Sectional Study ◽  
High Rate ◽  
Syphilis Infection ◽  
Cross Sectional ◽  
History Of ◽  
Treponemal Infection ◽  
Active Syphilis

A rural population of east Senegal has been under demographic surveillance for more than 30 years and a high rate of infertility has been reported. The aim of the study is to describe HIV and treponemal infection epidemiology and association with outcome of pregnancy in a population of rural Senegal. A population-based cross-sectional study was conducted among 952 randomly-selected adults of a rural community of Senegal. No participant was found to be infected with HIV, 11% had evidence of past syphilis and 5% of active syphilis. Active syphilis was associated among men with age, long-term mobility and having partners in an urban area in the last 12 months and among women with being divorced or widowed. No association was found between past or active syphilis and abortion or stillbirth but women aged 40 and more with past or active syphilis were significantly more likely to have had no history of gestation than women with no evidence of syphilis infection. In conclusion our results call for more research to understand the epidemiology of treponemal infection and to elucidate types of Treponema pallidum involved.

scholarly journals Assessment of syphilis infection among pregnant women and its associated risk factors at Sovu health center in Rwanda

2020 ◽  
Vol 1 (3) ◽  
Author(s):  
Augustin Nshimiyimana ◽  
Joseph Mucumbitsi ◽  
Callixte Yadufashije ◽  
Francois N. Niyonzima
Keyword(s):  
Risk Factors ◽  
Pregnant Women ◽  
Health Center ◽  
Sexual Behaviors ◽  
Treponema Pallidum ◽  
Health Concern ◽  
Syphilis Infection ◽  
Cross Sectional ◽  
History Of ◽  
Associated Risk Factors

Syphilis in pregnant women caused by Treponema pallidum remains a major cause of reproductive morbidity and poor pregnancy outcomes in developing countries. Severe neonatal infections, stillbirths, perinatal deaths, and low birth weight babies are common among mothers with syphilis infection. The aim of the study was to assess the prevalence and risk factors associated to syphilis in pregnant women. A cross-sectional and retrospective studies were conducted among pregnant women who attended the prenatal service at the Gisovu health center. Participants were pregnant women who tested positive for syphilis based on lab results and responses collected from anonymous questionnaires completed with sexual behaviors, demographics, sexual partners, history of abortion, and knowledge about STDs. The prevalence of syphilis was 5.74%. Syphilis was associated to the history of previous abortion (P = 0.005 < 0.05), a low level of education (P = 0.049 < 0.05), and marital status (P = 0.044 < 0.05). The main associated behavioral factor was women who had sex with different partners using condom and did not acquire syphilis infection (P = 0.00 < 0.05). Syphilis is still a public health concern in patients and especially in pregnant women as shown by the findings of this study. It is very important to screen all pregnant women for syphilis and to strengthen the existing antenatal care services and health education on transmission and prevention of the disease.

scholarly journals Depression, anxiety levels and sleep quality indexes among the spouses of people with epilepsy

2021 ◽  
Author(s):  
Halil ONDER ◽  
Ersin Kasim ULUSOY ◽  
Caner BAYDAR ◽  
Mustafa KIRAZ ◽  
Muhammet Okay ORUN ◽  
...  
Keyword(s):  
Sleep Quality ◽  
Sleep Disturbances ◽  
Demographic Data ◽  
Cross Sectional Study ◽  
Control Group ◽  
Cross Sectional ◽  
History Of ◽  
Group 3 ◽  
Group 2 ◽  
Psychiatric Problems

ABSTRACT Background: Psychiatric problems and sleep disturbances are comorbidities that are frequently encountered among people with epilepsy. However, their presence among the spouses of peoples with epilepsy remains to be elucidated. Objective: The objective of this study was to evaluate the spouses of people with epilepsy (PWE), with and without a history of seizures during sleep, in terms of depression, anxiety and sleep quality. Methods: This prospective, cross-sectional study was conducted in three groups of 18 to 65-year-olds. Group 1 consisted of healthy spouses of 127 healthy volunteers without any known neurological disease; group 2 comprised spouses of 63 PWE who had no history of seizure during sleep; and group 3 consisted of spouses of 63 PWE who had a history of at least one seizure during sleep in the course of the previous year. Questionnaires seeking demographic data and the Pittsburgh Sleep Quality Index (PSQI), Beck Depression Inventory and Beck Anxiety Inventory were applied to all participants. Results: The depression scores of the group of spouses of PWE were higher than those of the control group and were higher in group 3 than in group 2 (p = 0.017). The anxiety scores of the group of spouses of PWE were significantly higher than those of the control group, but no difference in anxiety scores was found between group 2 and group 3 (p = 0.170). The mean PSQI score of group 3 was higher than that of group 2 (p = 0.029). However, regression analyses did not show any difference between these groups. Conclusion: We found that the PSQI scores, which reflected sleep quality, were higher among the spouses of PWE who had seizures during sleep and who had more severe epilepsy.

scholarly journals Determinants of anemia among pregnant women: A hospital based cross sectional study

2019 ◽  
Vol 8 (9) ◽  
pp. 3609
Author(s):  
P. Kalpana ◽  
A. Kavitha
Keyword(s):  
Pregnant Women ◽  
Demographic Data ◽  
Cross Sectional Study ◽  
Hemoglobin Level ◽  
Sectional Study ◽  
Cross Sectional ◽  
Lower Segment Cesarean Section ◽  
Menstrual History ◽  
History Of ◽  
The Mean

Background: The objective was to study the determinants of anemia among pregnant women. There is a negative effect on the health of the mother and as well as that of the child due to anemia in women with pregnancy. Death rate is more in women who are pregnant and having anemia. The objective of this study was to study the determinants of anemia among pregnant women.Methods: A hospital based cross sectional study was carried out among 40 pregnant women over a period of six months. Demographic data like age, residence, occupation etc was recorded. Obstetric data like gravida, parity, previous LSCS was also recorded. The data was presented as means and student’s t test was applied.Results: The mean age was 23.43±3.4 years. Majority were young between the ages of 19-22 years. Majority belonged to urban residents i.e. 57.5%. 20% of the subjects were found to be illiterate. Majority of the mothers were housewives i.e. 82.5%. Majority were multi-gravida i.e. 60%. Majority had no history of abortions in the past i.e. 75%. Out of total 19 repeat pregnancies, majority i.e. 84.2% had lower segment cesarean section. Majority reported that they had normal menstrual history. The mean hemoglobin level was found out to be 7.94 gm/dl. It was found that the mean hemoglobin level did not differ significantly across age, residence, education, occupation, gravidity, history of abortions, type of delivery but differed significantly by abnormal menstrual cycle.Conclusions: Mean hemoglobin level was more in women with normal menstrual history compared to those women with abnormal menstrual history and this difference was found out to be statistically significant. Hence abnormal menses should be promptly treated, hemoglobin assessed.

scholarly journals Prevalence of cesarean section on demand in Assiut Governorate, Egypt

2019 ◽  
Vol 8 (4) ◽  
pp. 1223
Author(s):  
Mahmoud S. Zakherah ◽  
Tarek A. Farghaly ◽  
Elham S. Ahmed ◽  
Ahmed M. Abbas
Keyword(s):  
Cesarean Section ◽  
Demographic Data ◽  
Previous Experience ◽  
Central Hospital ◽  
Fear Of Pain ◽  
Cross Sectional ◽  
On Demand ◽  
Significant Difference ◽  
History Of ◽  
Health Hospital

Background: The current study aims to evaluate the prevalence of CS on demand in Women's health hospital, Assiut University and Abnob Central Hospital in Assiut Governorate, Egypt.Methods: A cross sectional study conducted in Assiut Women Health Hospital and Abnob central hospital from January 2017 to December 2017. The total number of cesarean section done was 180 cases and the number of CS on demand was 64 (35.6%). The demographic data were collected by one of the study investigators. Women were asked about the causes of requesting CS before surgery.Results: The study group was 64 women with age ranging from 18-40 years old, 40 primipara and 24 multipara. Of those 24 women, 21 of them previously delivered vaginally and only 3 women delivered by emergency CS. Twenty- six women had a history of previous abortion. Fear of pain was the main cause for CS on demand in the whole study participants (57.8%). In primipara, the main cause for requesting CS is fear of pain in 62.5% of participants followed by fear on the baby in 45 % of women. On the other hand, in multipara, the main cause for CS on demand was bad history of previous experience (60%) followed by fear of pain in 50% of cases. There was statistical significant difference between both groups in only two causes; fear of pelvic floor injuries (50% in multipara vs. 20% in primipara, p=0.02) and bad history of previous experience (60% in multipara vs. 0% in primipara, p=0.001). Other causes were not statistically different.Conclusions: The incidence of cesarean sections performed on request without medical indications is rising. The reasons for this are not only for perceived medical benefit, but also due to social, cultural, and psychological factors.

Factors contributing to intentional injuries in Iranian university students

2017 ◽  
Vol 31 (3) ◽  
Author(s):  
Kobra Abedian Kasgary ◽  
Zeinab Hamzehgardeshi ◽  
Zohreh Shahhosseini
Keyword(s):  
University Students ◽  
Demographic Data ◽  
Human Action ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Intentional Injuries ◽  
Significant Difference ◽  
History Of ◽  
And Gender ◽  
The University

Abstract Background Intentional injuries refer to injuries resulting from purposeful human action, whether directed at oneself or others. This study was performed to assess intentional injuries in Iranian university students. Methods This cross-sectional study was carried out with 430 female and male university in three higher education institutions located in the northern part of Iran in the year 2015. Samples were chosen through the stratified cluster random sampling method. They were requested to fill out the demographic data form and the Persian version of the Youth Risk Behavior Survey Questionnaire. Descriptive and inferential statistics were used for data analysis via the SPSS v.13 software. Findings Intentional injuries were more frequent in the male university students than female (p < 0.05). Also, 9.1% and 6.7% of the university students were physically injured or sexually assaulted by a boy/girl friend. No statistically significant difference was reported in dating violence between the male and female university students. The logistic regression test showed that the history of stealing money from parents without their permission, son’s preferences in the family and gender are the most important predisposing factors for the university students’ intentional injury. Conclusion It is suggested that health policy makers consider the role of family in programs that have been designed for improving the health of young people.

scholarly journals Seroepidemiological Study of Toxocariasis in Children Aged 6-14 Year Old in Sanandaj, Western Iran

2020 ◽  
Author(s):  
Yahya MAROUFI ◽  
Ashkan FARIDI ◽  
Mohammadbagher KHADEMERFAN ◽  
Fares BAHRAMI ◽  
Ghasem ZAMINI
Keyword(s):  
Demographic Data ◽  
Clinical Signs ◽  
Cross Sectional Study ◽  
Larva Migrans ◽  
Serum Samples ◽  
Igg Antibody ◽  
Cross Sectional ◽  
Medical Diagnostic ◽  
History Of ◽  
Low Prevalence

Background: Toxocariasis is a disease caused by Toxocara nematodes and occurs from consuming their eggs. The main hosts of these worms are dogs and cats. The disease in humans becomes a visceral larva migrans (VLM). This descriptive cross-sectional study was conducted to determine the prevalence of toxocariasis in children aged 6–14 years. Methods: This cross-sectional descriptive study was conducted from Jun 1 2016 to Dec 1 2017 in Sanandaj, west of Iran. A total of 182 serum samples were collected from children age 6 to14 yr referred to medical diagnostic laboratories. Demographic data (age, sex, and parents' literacy status), clinical signs (cough, headache, fever, abdominal pain), and the history of contact with dogs and cats was collected by a questionnaire. The presence of anti-Toxocara IgG antibody was detected by T. canis IgG ELISA (IBL, Germany) kit. Results: Of 182 subjects, 97 (53.3%) were male and 85 (46.7%) female. The average age was 9.2 years. Antibodies against T. canis were positive in three cases (1.65%) of all the studied subjects. Conclusions: The results showed a low prevalence of toxocariasis in children studied.

scholarly journals Study reasons and motives women tend to Rhinoplasty in Ardabil city

2017 ◽  
Vol 4 (4) ◽  
pp. 1014
Author(s):  
Anahita Zakeri ◽  
Firouz Amani ◽  
Roghayeh Aslanian ◽  
Vahid Abbasi ◽  
Habib Ojaghi
Keyword(s):  
Cosmetic Surgery ◽  
Total Population ◽  
Demographic Data ◽  
Cross Sectional Study ◽  
Main Role ◽  
Age Group ◽  
Cross Sectional ◽  
Self Confidence ◽  
The World ◽  
History Of

Background: Cosmetic surgery known as prevalent surgeries in the world that annually thousands of people are undergoing cosmetic surgery to change their appearance. Each year more than 200000 cosmetic surgery have been done on patients 18 years and low and about 390000 of them included surgeries such as breast, cosmetic, abdomen and nose. Iran in terms of plastic surgeries performed proportion to total population is located in the first rank of the world. Studies showed that social and mental factors have main role in doing these surgeries. So, the aim of this study was to evaluate the reasons and motives women tend to Rhinoplasty in Ardabil city.Methods: This descriptive-cross sectional study has been done on 120 women 15-50 years referred to cosmetic surgery clinics and centers. Samples selected randomly from centers which doing Rhinoplasty and data collected by a research base questionnaire including information about demographic data, causes and motive of women tend to Rhinoplasty.Results: Most of women were in the age group 15-20 (39.1%). 56.7% of women were married and 45% were housekeeper. 15% of women have history of psychological diseases and 58.3% have history of doing surgery in their family.Conclusions: Results showed that self confidence known as important factor in Rhinoplasty. Personality characteristics, social conditions and environmental factors play an important role in women tend to cosmetic surgery. So, we need doing studies in big samples for recognize the dimensions of effective factors in doing Rhinoplasty among women.

scholarly journals Cataract and its risk factor among adults residing in South Bihar, India

2020 ◽  
Vol 8 (7) ◽  
pp. 2618
Author(s):  
Shiwani Gupta ◽  
Animesh Gupta ◽  
M. Nehal ◽  
Kalyani Pandey ◽  
Ananta Kreesna ◽  
...  
Keyword(s):  
Family History ◽  
Urban Areas ◽  
Dietary Habits ◽  
Demographic Data ◽  
Sun Exposure ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Age Related ◽  
History Of

Background: Cataract is considered as one of the most common causes of visual impairment and the leading cause of blindness in the world. Age related cataract occurs in people above 50 years of age and the its pathogenesis is multifactorial. Therefore, the present study was aimed to assess the prevalence and risk factors for cataract.Methods: Community based cross sectional study was conducted among adults residing at urban areas of South Bihar. The data was collected by interview method, using pre-tested semi-structured questionnaire which contains socio-demographic data, dietary habits, history of diabetes or hypertension, family history of cataract and long-term sun exposure.Results: Among 240 participants, 94 (39.2%) were male and 146 (60.8%) were female. Maximum participants were aged more than 70 years. The prevalence of cataract was 52.1%.Conclusions: The prevalence of cataract was quite high and was significantly associated with age, long term sun exposure and family history of cataract.

scholarly journals CORRELATION BETWEEN HISTORY OF MATERNAL ANEMIA AND PREVALENCE OF 0-5 YEARS OLD STUNTING CHILDREN IN CILEMBU VILLAGE, SUMEDANG REGENCY, 2019

2021 ◽  
Vol 9 (4) ◽  
pp. 256-262
Author(s):  
Batara Imanuel Sirait ◽  
Wiradi Suryanegara
Keyword(s):  
Pregnant Women ◽  
Health Problem ◽  
Pearson Correlation ◽  
Demographic Data ◽  
Public Health Problem ◽  
Intellectual Ability ◽  
West Java ◽  
Cross Sectional ◽  
Maternal Anemia ◽  
History Of

Stunting is a health problem among children in Indonesia, reaching 30,8% and 29,9% on infants less than five years old and two years old. West Java prevalence of stunting is higher than 38,8% of the entire stunting population in Indonesia in 2018. ranks second in districts with the highest prevalence of stunting in West Java, which is 19,4%, according to in 2013. Stunting caused major problems are delayed mental development, lack of intellectual ability and long-term consequences of degenerative diseases. is a public health problem that burdens developed and developing countries, especially adolescents and pregnant women. Risked data reaches 48.9% of incidence on pregnant women. Maternal can increase the prevalence of stunting. The purpose of this study to determine the Correlation between the History of Maternal Anemia and Prevalence of 0-5 Years Old Stunting Children in Cilembu Village, Sumedang Regency, January 2019 Period. This study used a cross-sectional analysis method, a sample of 61 consisted of stunting children aged 0-5 years in Columbus Village. Statistics is tested by Pearson correlation and also analysis of demographic data. The prevalence of maternal 50,8%, and the highest category of stunting is very short (46%). The results of Pearson's analysis found (p= 0.717 &gt; 0.05) that do not have an association between the History of Maternal Anemia and the incidence of stunting aged 0-5 years old. There is no correlation between the History of Maternal Anemia and the incidence of stunting aged 0-5 years old in Cilembu Village, Sumedang Regency, January 2019 Period.

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