Application of the hydrotime model to assess seed priming effects on the germination of rapeseed (Brassica napus L.) in response to water stress

Our aim was to determine whether the parameters of the hydrotime model can be used to compare the effects of seed priming treatments on germination. Seeds of three rapeseed cultivars (‘H61’, ‘H4815’, and ‘DK003’) were exposed to priming treatments and then their germination was tested at different water potentials. Seed priming improved the germination percentage, which was higher in ‘H4815’ than in the other two cultivars, and it increased germination rate (decreased θH) and uniformity (decreased σψb). The increase in germination rate was higher in PEG-osmoprimed than in NaCl-osmoprimed and hydroprimed seeds. The base water potential allowing 50% germination ([Formula: see text]) also was affected by seed priming, but the response of cultivars to different priming treatments varied. Compared with the control seeds, hydropriming of ‘H61’ seeds shifted ψb(50) toward negative values, whereas NaCl-osmopriming of ‘H4815’ seeds shifted ψb(50) toward negative values. On the other hand, priming of ‘DK003’ seeds shifted ψb(50) to more positive values and increased susceptibility to water stress. After dormancy-breaking and dormancy-induction treatments, ψb(50) shifted to more negative or positive values, indicating the release of primary dormancy and induction of dormancy, respectively. Therefore, priming treatments should be selected, depending on the environmental conditions anticipated to prevail during the germination of rapeseed seeds.

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Hydrotime model to evaluate the effects of a set of priming agents on seed germination of two leek cultivars under water stress

Seed Science and Technology ◽

10.15258/sst.2021.49.2.07 ◽

2021 ◽

Author(s):

Alessandro Romano ◽

Romana Bravi

Keyword(s):

Water Stress ◽

Water Deficit ◽

Germination Rate ◽

Intraspecific Variability ◽

Germination Percentage ◽

Allium Ampeloprasum ◽

Cultivated Species ◽

Hydrotime Model ◽

Base Water Potential ◽

Peg 8000

Water deficit is one of the main constraints of germination for many cultivated species. Among the techniques used to promote germination, seeds priming has been found to play a key role. In the present study, a set of different priming agents (KCl, KH2PO4, CaCl2 and PEG 8000) was tested on seeds of two leek (Allium ampeloprasum L.) cultivars (“Lungo della riviera” and “Monstrueux de Carentan”) to assess the effects on germination performances both in optimal hydration condition (Ψw = 0 MPa) and under water deficit (−0.3 and −0.6 MPa). Germination percentage of “Lungo della riviera” was improved by PEG and KCl at −0.3 MPa and −0.6 MPa, respectively, whereas CaCl2 improved germination of “Monstrueux de Carentan” at −0.6 MPa. Osmopriming was beneficial in terms of germination rate although to different extent depending on genotype and priming agent. The hydrotime model showed that the effects of priming on germination rate was mostly due to a lowering of the predicted hydrotime constant (θH), whereas median base water potential Ψb(50) tended to increase following treatments albeit with some exception. In this study, hydrotime model proved to be a useful tool to test the effectiveness of different priming treatments on leek seeds and the intraspecific variability in germination capability and speed under water stress.

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Hydropriming accelerates seed germination of Medicago sativa under stressful conditions: A thermal and hydrotime model approach

Legume Research - An International Journal ◽

10.18805/lr.v0i0.8404 ◽

2017 ◽

Author(s):

Rong Li ◽

Dandan Min ◽

Lijun Chen ◽

Chunyang Chen ◽

Xiaowen Hu

Keyword(s):

Water Potential ◽

Germination Rate ◽

High Water ◽

Nacl Stress ◽

Base Temperature ◽

Severe Water Stress ◽

Hydrotime Model ◽

Response To Temperature ◽

Base Water Potential ◽

Temperature Water

This study determined the effects of priming on germination in response to temperature, water potential and NaCl. Thermal and hydrotime models were utilized to evaluate changes in parameters of the model after priming. Priming reduced the amount of thermal time in both cultivars, but slightly increased the base temperature for germination from 1.0 to 3.5°C in “Longdong”. Priming significantly increased germination rate at high water potential but had no effect at low water potential. Further, priming reduced the hydrotime constant but made the median base water potential value slightly more positive in both cultivars. Thus, priming increased germination rate in water but decrease it under severe water stress. Germination rate was significantly increased in both cultivars under salinity (NaCl) stress. Moreover, priming improved seedling growth in response to temperature, water and salinity stress in both cultivars.

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Effect of Seed Priming on Germination Properties and Seedling Establishment of Cowpea (Vigna sinensis)

Notulae Scientia Biologicae ◽

10.15835/nsb346338 ◽

2011 ◽

Vol 3 (4) ◽

pp. 113-116 ◽

Cited By ~ 6

Author(s):

Hamdollah ESKANDARI ◽

Kamyar KAZEMI

Keyword(s):

Laboratory Tests ◽

Low Cost ◽

Germination Rate ◽

Seedling Emergence ◽

Laboratory Data ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Seedling Vigor ◽

Contributing Factors

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.

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Osmotic Seed Priming of Rudbeckia fulgida Improves Germination and Expands Germination Range

HortScience ◽

10.21273/hortsci.29.8.868 ◽

1994 ◽

Vol 29 (8) ◽

pp. 868-870 ◽

Cited By ~ 2

Author(s):

Amy M. Fay ◽

Mark A. Bennett ◽

Steven M. Still

Keyword(s):

Polyethylene Glycol ◽

Germination Rate ◽

Seed Priming ◽

Germination Percentage ◽

The Mean ◽

Mean Time

Low-vigor seeds of black-eyed Susan (Rudbeckia fulgida Ait.) primed in aerated -1.3 MPa KNO3 for 7 days at 30C in darkness had double the total germination percentage at 30C and one-half the mean time of germination as nonprimed seeds. Priming the seeds in polyethylene glycol rather than KNO3 generally resulted in lower total germination percentage and longer mean time of germination. Osmotic priming increased total germination percentage and germination rate of seeds germinated at 21.9 to 32.2C, but the priming benefit on total germination percentage was greater at ≤27.6C. Total germination percentage of primed and nonprimed seeds was highest at 28.8 to 32.2C.

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Physiological quality of osmoprimed Eremanthus erythropappus (D.C.) MacLeish seeds

Scientific Electronic Archives ◽

10.36560/1352020919 ◽

2020 ◽

Vol 13 (5) ◽

pp. 18

Author(s):

A. M. S. Oliveira ◽

A. A. Silva ◽

M. C. Vasconcelos ◽

J. A. A. Granja ◽

J. M. R. Faria ◽

...

Keyword(s):

Polyethylene Glycol ◽

Germination Rate ◽

Seed Priming ◽

Potassium Nitrate ◽

The Other ◽

Other Substances ◽

Physiological Quality ◽

Glycol Solution ◽

Polyethylene Glycol 6000

Physiological conditioning is a technique that consists in controlling the speed of water uptake by seeds, with osmotic solutions, aiming to improve their quality, reducing time and increasing germination rate. Eremanthus erythropappus (D.C.) MacLeish seeds were subjected to priming with polyethylene glycol 6000 (PEG), potassium nitrate (KNO3) and the combination of the two at the concentrations of 0.4; -0.8; -1.0; -1.2 and -1.4 MPa for four, six and eight days. Germination, germination speed index (GSI), emergence, emergence speed index (ESI) and expression of superoxide dismutase (SOD), catalase (CAT) and peroxidase (PO) enzymes were assessed. E. erythropappus seed priming in polyethylene glycol solution with potentials between -0.8 MPa and -1.4 MPa is not satisfactory because it adversely affects germination and reduces the activity of peroxidase and catalase enzymes. The other substances were not significant so there is a need for further research with other substances and / or concentrations

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The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽

10.3390/agriculture10040094 ◽

2020 ◽

Vol 10 (4) ◽

pp. 94

Author(s):

Iman Mirmazloum ◽

Attila Kiss ◽

Éva Erdélyi ◽

Márta Ladányi ◽

Éva Zámboriné Németh ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Germination Rate ◽

Petri Dish ◽

Seed Priming ◽

Germination Percentage ◽

Positive Effects ◽

Carum Carvi ◽

Early Seedling ◽

Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

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Germinability and Seedling Growth Performance of Chilli (Capsicum annuum) Seeds in Response to Different Gibberellic Acid Concentrations Pre-Treatment

AgroTech Food Science, Technology and Environment ◽

10.53797/agrotech.v1i1.2.2021 ◽

2021 ◽

Vol 1 (1) ◽

pp. 10-16

Author(s):

Ryn Brenda Junaidy ◽

Shafeeqa Shahruddin

Keyword(s):

Gibberellic Acid ◽

Growth Performance ◽

Seedling Growth ◽

Crop Production ◽

Germination Rate ◽

Germination Percentage ◽

Dormancy Breaking ◽

Seedling Vigour ◽

Seed Germinability ◽

Significant Difference

Chilli seeds are always associated with physiological dormancy characteristics or endogenous dormancy that has affected their germination potential. Problems noticed on the low seed germination rate, vigour growth, further may lead to the poor seedling growth pattern of chilli crop production. Gibberellic Acid (GA3) which is usually used as a treatment solution is not a new advent of seed dormancy breaking methods for chilli. But, looking forwards to a better GA3 concentration is still needed for the discovery of this treatment potential effect on chilli seed germinability and seedling growth. Thus, this study aimed to evaluate the effect of different GA3 concentrations on seed emergence and seedling growth performance in chilli. In this experiment, chilli seeds were imbibed with 25 mg/L, 75 mg/L and 125 mg/L concentration of GA3 with three replications each; for 24 hours at room temperature (32±4°C) and the untreated seeds as a control. The data collection on final germination percentage was taken daily for 21 days after sowing. While the 15 germinated seeds were directly planted on the 5 polybags arranged in the net house of Junaidy Jonik Farm, Sabah, Malaysia. The experiment was arranged in a completely randomized design with three replications. Data were subjected to analysis of variance with SAS version 9.4 and the significant means were separated by the least significant difference test at P<0.05. Significant differences were observed in the seed germinability measured; germination rate index (GRI), mean germination time (MGT) and final germination percentage (FGP), as well as on seedling vigour index (SVI) between treated and control seeds. Conversely, plant height, number of internodes, number of leaves and fresh weight of seedlings showed no significant differences among treatments. It was concluded that the use of GA3 was able to enhance chilli seeds germinability and could display a better SVI than the control. It is recommended to use GA3 treatment at 25 mg/L of concentration, as it may give an advantage to both economic and biological importance in producing higher germinability and seedling growth performance in chilli.

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Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

International Journal of Life Sciences ◽

10.3126/ijls.v9i1.11919 ◽

2015 ◽

Vol 9 (1) ◽

pp. 6-11

Author(s):

Abolfazl Masoumi Zavariyan ◽

Mojtaba Yousefi Rad ◽

Mohsen Asghari

Keyword(s):

Drought Stress ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Silybum Marianum ◽

Randomized Design ◽

Seedling Length ◽

Germination Indices

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

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Muskmelon seed priming in relation to seed vigor

Scientia Agricola ◽

10.1590/s0103-90162004000100019 ◽

2004 ◽

Vol 61 (1) ◽

pp. 114-117 ◽

Cited By ~ 20

Author(s):

Warley Marcos Nascimento ◽

Fernando Antônio Souza de Aragão

Keyword(s):

Low Temperature ◽

Interaction Effect ◽

Cucumis Melo ◽

Seed Quality ◽

Germination Rate ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Cucumis Melo L ◽

Aged Seeds

A number of important factors may affect seed priming response, including seed quality. Effects of seed vigor on seed priming response were investigated using seed lots of two muskmelon (Cucumis melo L.) cultivars. Seeds of muskmelon, cvs. Mission and Top Net SR were artificially aged at 43°C for 0, 20 and 40 hours. Seeds were primed for six days in darkness at 25°C in KNO3 (0.35 mol L-1) aerated solution. Aged seeds germinated poorly at 17°C. Priming increased germination rate at 17 and 25°C and germination percentage at 17°C. An interaction effect on germination performance between vigor and priming was observed, especially at low temperature. Priming increased germination performance in seeds of low vigor, and the response was cultivar dependent.

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Potasssium chloride as a nutrient seed primer to enhance salt‑tolerance in maize

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2012000800020 ◽

2012 ◽

Vol 47 (8) ◽

pp. 1181-1184 ◽

Cited By ~ 4

Author(s):

Badar‑uz‑Zaman ◽

Arshad Ali ◽

Syed Ishtiaq Hyder ◽

Muhammad Arshadullah ◽

Saqib Umar Bhatti

Keyword(s):

Salt Stress ◽

Salinity Stress ◽

Dry Matter ◽

Germination Rate ◽

Seed Priming ◽

Germination Percentage ◽

Stress Conditions ◽

Maize Crop ◽

Mean Germination Time ◽

Muriate Of Potash

The objective of this work was to determine if KCl could be a useful nutrient primer for safe seed germination in maize crop under salt stress conditions. Seed priming was done using 50 mmol L‑1 of muriate of potash, and germination and seedling growth were evaluated after salt stress with NaCl up to 50 mmol L‑1. Another set of seeds was tested under the same salt stress conditions without priming. Under salinity stress, germination percentage, germination rate index, germination coefficient, and seedling vigor indexes were higher in primed seeds. In unprimed seeds, mean germination time increased, while the germination rate index and the fresh and dry matter mass decreased more sharply with salinity stress. The Na/K ratio was higher in unprimed seeds.

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