TRANSGENERATIONAL IMPACT OF TOPICAL STEROID APPLICATION ON SUPEROXIDE DISMUTASE ACTIVITIES OF HYPOTHALAMUS-PITUITARY-ADRENAL AXIS IN RATS

2021 ◽  
Author(s):  
Seher Polat ◽  
Armağan Caner
Keyword(s):  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Early Adulthood ◽  
Topical Steroid ◽  
Female Rats ◽  
Male Rats ◽  
Topical Steroids ◽  
Antioxidant Mechanism ◽  
First Time ◽  
Pituitary Adrenal Axis

Topical steroids(TS) are widely prescribed since the 1950s.This study aimed to investigate the transgenerational effects of TS on the antioxidant mechanism of the hypothalamus-pituitary-adrenal(HPA) axis,both in prenatal and infancy for the first time. Three generations(F1, F2 and F3) and prenatal group(P) were investigated in both sexes with two different time points; P45th and P75th day were accepted as puberty and early adulthood,respectively.Clobetasol propionate 0.05% was used as TS.qRT-PCR was performed to expressional analyses of Sod1, Sod2, and Sod3 genes in the HPA tissues. The Sods mRNA expression of the HPA belonging to P and F1 groups revealed similar results in both genders. The downregulation in the adrenal Sod level was determined in P and F1, F2, and F3 generations in both gender, especially in females(p<0.05).Sods activities in the pituitary of all groups were downregulated in female rats(p<0.05).Interestingly,in male rats,Sod2 and Sod3 weren’t expressed in the pituitary compare to control on the day P45 while Sod2 and Sod3 expressions were determined in all the groups on the day P75.Sod1 overexpression found in pituitary and hypothalamus of male in F3 generation. This study showed that TS applied in infancy had a transgenerational adverse effect on antioxidant defense mechanisms especially in the adrenal.

Transgenerational effects of a hypercaloric diet

2017 ◽  
Vol 29 (2) ◽  
pp. 325 ◽  
Author(s):  
A. O. Joaquim ◽  
C. P. Coelho ◽  
P. Dias Motta ◽  
E. F. Bondan ◽  
E. Teodorov ◽  
...  
Keyword(s):  
Early Adulthood ◽  
Inflammatory Cells ◽  
Female Rats ◽  
Male Rats ◽  
Transgenerational Effect ◽  
Gfap Immunoreactivity ◽  
Factor Α ◽  
Hypercaloric Diet ◽  
Necrosis Factor ◽  
F1 Generation

The effects of a maternal hypercaloric diet (HD) during puberty and early adulthood on neuroimmune aspects in offspring were investigated. In female rats of the F0 generation and male rats of the F1 generation, bodyweight (BW) gain, retroperitoneal fat (RPF) weight, the number of hypodermic adipocytes (HAs) and expression of glial fibrillary acidic protein (GFAP) were measured in hypothalamic astrocytes. On Postnatal Day 50, the F1 pups were challenged with lipopolysaccharide (LPS, 100 µg kg–1, s.c.) or an equal volume of saline (S), and behaviour in the open field test was evaluated, as were plasma neuropeptide and cytokine concentrations. The maternal HD caused the female F0 rats to become overweight. The F1 offspring of dams fed the HD and challenged with saline (HDS group) exhibited increases in BW gain, RPF weight and in the number of large HAs and a decrease in GFAP immunoreactivity. F1 offspring of dams fed the HD and challenged with LPS (HDLPS group) exhibited decreases in BW gain, RPF weight and GFAP immunoreactivity, but no differences were observed in the number of larger and small HAs. Plasma tumour necrosis factor-α concentrations were high in the HDS and HDLPS groups. Thus, the maternal HD during puberty and early adulthood caused the F1 generation to become overweight despite the fact that they received a normocaloric diet. These results indicate a transgenerational effect of the HD that may occur, in part, through permanent changes in immune system programming. The attenuation of neuroinflammation biomarkers after LPS administration may have resulted in a decrease in the number of adipocytes, which, in turn, reduced cytokine, adipokine and chemokine levels, which are able to recruit inflammatory cells in adipose tissue.

scholarly journals Sex Differences in Pubertal Circadian and Ultradian Rhythmic Development Under Naturalistic Conditions

2021 ◽  
Author(s):  
Azure Dominique Grant ◽  
Linda Wilbrecht ◽  
Lance J Kriegsfeld
Keyword(s):  
Sex Differences ◽  
Temporal Structure ◽  
Pubertal Development ◽  
Early Adulthood ◽  
Female Rats ◽  
Male Rats ◽  
Vaginal Opening ◽  
Field Station ◽  
Pubertal Onset ◽  
Laboratory Conditions

Biological rhythms in core body temperature (CBT) provide informative markers of adolescent development under controlled laboratory conditions. However, it is unknown if these markers are preserved under more variable naturalistic conditions, and if CBT may therefore prove useful in a real-world setting. To evaluate this possibility, we examined fecal steroid concentrations and CBT rhythms from pre-adolescence (p26) through early adulthood (p76) in intact male and female rats under natural light and climate at the University of California, Berkeley Field Station. Despite greater environmental variability, CBT markers of pubertal onset and its rhythmic progression were comparable to those previously reported in laboratory conditions in female rats and extend actigraphy-based findings in males. Specifically, sex differences emerged in circadian rhythm (CR) power and temperature amplitude prior to pubertal onset and persisted into early adulthood, with females exhibiting elevated CBT and decreased CR power compared to males. Within-day (ultradian rhythm; UR) patterns also exhibited a pronounced sex difference associated with estrous cyclicity. Pubertal onset, defined by vaginal opening, preputial separation, and sex steroid concentrations, occurred later than previously reported under lab conditions for both sexes. Vaginal opening and increased fecal estradiol concentrations were closely tied to the commencement of 4-day oscillations in CBT and UR power in female rats. By contrast, preputial separation and the first rise in testosterone concentration were not associated with adolescent changes to CBT rhythms in male rats. Together, males and females exhibited unique temporal patterning of CBT and sex steroids across pubertal development, with tractable associations between hormonal concentrations, external development, and temporal structure in females. The preservation of these features outside the laboratory supports CBT as a strong candidate for translational pubertal monitoring under naturalistic conditions in females.

Growth hormone secretory profiles in conscious female rats

1987 ◽  
Vol 114 (3) ◽  
pp. 399-407 ◽  
Author(s):  
R. G. Clark ◽  
L. M. S. Carlsson ◽  
I. C. A. F. Robinson
Keyword(s):  
High Amplitude ◽  
Female Rats ◽  
Male Rats ◽  
Ovariectomized Rats ◽  
Normal Female ◽  
Gh Secretion ◽  
First Time ◽  
Conscious Animals ◽  
Secretory Pattern ◽  
Almost Continuous

ABSTRACT An automatic method for repetitive microsampling of blood from conscious animals was used to obtain detailed GH secretory profiles from normal female rats, which were compared with those in males and ovariectomized females. Female rats showed a highly variable GH secretory pattern, with sustained periods of low, almost continuous secretion, followed by very rapid bursts of high amplitude and short duration, occurring mostly at night. There was no clear relationship between the pattern of GH secretion and the phase of the oestrous cycle in rats continuously sampled over a 5-day period. In ovariectomized rats, the day:night difference was maintained, though the nocturnal GH surges were larger and of longer duration than in intact females. Male rats produced multicomponent GH bursts which continued unchanged throughout the day and night. This study shows for the first time that female rats switch to a rapid, highly pulsatile pattern of GH release at night, which can only be resolved by rapid blood sampling over extended periods in conscious undisturbed animals. J. Endocr. (1987) 114, 399–407

A CORRELATIVE STUDY OF ENDOCRINE RHYTHMS IN RATS

1968 ◽  
Vol 57 (4) ◽  
pp. 615-622 ◽  
Author(s):  
K. Retiene ◽  
E. Zimmerman ◽  
W. J. Schindler ◽  
J. Neuenschwander ◽  
H. S. Lipscomb
Keyword(s):  
Relevant Literature ◽  
Plasma Corticosterone ◽  
Light Period ◽  
The Other ◽  
Female Rats ◽  
Male Rats ◽  
Peak Time ◽  
Plasma Acth ◽  
Hypothalamic Tissue ◽  
First Time

ABSTRACT Several endocrine rhythms have been studied simultaneously in the same group of rats under resting conditions. A parallel circadian periodicity was found for adrenal and plasma corticosterone, pituitary and plasma ACTH, and for the first time, for pituitary vasopressin. These rhythms are typical for nocturnal animals, demonstrating a distinct peak before the onset of darkness and lowest values at the beginning of the light period. An opposite behaviour was found for pituitary TSH in male rats showing a significant fall at the peak time of the other hormones, whereas in female rats this fluctuation was not observed. No CRF-like material could be obtained from hypothalamic tissue under resting conditions. The methods used in this study and the results obtained are discussed together with the relevant literature.

scholarly journals Dietary linoleic acid requirements in the presence of α-linolenic acid are lower than the historical 2 % of energy intake value, study in rats

2015 ◽  
Vol 113 (7) ◽  
pp. 1056-1068 ◽  
Author(s):  
Benjamin Choque ◽  
Daniel Catheline ◽  
Bernadette Delplanque ◽  
Philippe Guesnet ◽  
Philippe Legrand
Keyword(s):  
Linoleic Acid ◽  
Linolenic Acid ◽  
Energy Intake ◽  
Human Subjects ◽  
Female Rats ◽  
Male Rats ◽  
Fatty Acid Deficiency ◽  
Set Up ◽  
First Time ◽  
Physiological And Biochemical

Previous studies on rats and human subjects have established that the linoleic acid (LA) requirement is 2 % of the total energy intake (en%), but is obtained in the absence of α-linolenic acid (ALA) and consequently appear to be overestimated. This raises questions since a recent study including ALA has suggested to divide the historical value by four. However, this recent study has remained inconclusive because the animals used were not totally LA-deficient animals. For the first time, the present study was especially designed using physiological and biochemical markers and performed in two steps: (1) to achieve a specific n-6 fatty acid deficiency model using growing male rats fed either a 0 en% from LA/0 en% from ALA (0LA/0ALA), 0LA/0·5ALA or 2LA/0·5ALA diet, born from female rats fed a 0LA/0·5ALA diet; and (2) to refine the required level of LA in the presence of ALA using rats fed either a 0LA/0ALA, 0·5LA/0·5ALA, 1LA/0·5ALA, 1·5LA/0·5ALA diet, born from female rats fed a 0LA/0·5ALA diet. The first step shows that the best LA deficiency model was obtained using rats fed the 0LA/0ALA diet, born from female rats fed the 0LA/0·5ALA diet. The second step demonstrates that in growing rats, LA deficiency was corrected with an intake of 1–1·5 en% from LA and 0·5 en% from ALA. These data suggest that the requirements in humans should be revisited, considering the presence of ALA to set up the recommendation for LA.

scholarly journals Sexual dimorphism and thyroid dysfunction: a matter of oxidative stress?

2014 ◽  
Vol 221 (2) ◽  
pp. R31-R40 ◽  
Author(s):  
Rodrigo S Fortunato ◽  
Andrea C F Ferreira ◽  
Fabio Hecht ◽  
Corinne Dupuy ◽  
Denise P Carvalho
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Sexual Dimorphism ◽  
Thyroid Dysfunction ◽  
Antioxidant Defense ◽  
Redox Balance ◽  
Female Rats ◽  
Male Rats ◽  
17Β Estradiol ◽  
Thyroid Dysfunctions

Thyroid diseases, such as autoimmune disease and benign and malignant nodules, are more prevalent in women than in men, but the mechanisms involved in this sex difference is still poorly defined. H2O2is produced at high levels in the thyroid gland and regulates parameters such as cell proliferation, migration, survival, and death; an imbalance in the cellular oxidant–antioxidant system in the thyroid may contribute to the greater incidence of thyroid disease among women. Recently, we demonstrated the existence of a sexual dimorphism in the thyrocyte redox balance, characterized by higher H2O2production, due to higher NOX4 and Poldip2 expression, and weakened enzymatic antioxidant defense in the thyroid of adult female rats compared with male rats. In addition, 17β-estradiol administration increasedNOX4mRNA expression and H2O2production in thyroid PCCL3 cells. In this review, we discuss the possible involvement of oxidative stress in estrogen-related thyroid pathophysiology. Our current hypothesis suggests that a redox imbalance elicited by estrogen could be involved in the sex differences found in the prevalence of thyroid dysfunctions.

INDUCTION OF GOITRE BY PTU OR KClO4 IN MALE AND FEMALE RATS. EFFECT OF GONADECTOMY

1973 ◽  
Vol 74 (1) ◽  
pp. 88-104 ◽  
Author(s):  
T. Jolín ◽  
M. J. Tarin ◽  
M. D. Garcia
Keyword(s):  
Iodine Content ◽  
High Plasma ◽  
Disc Electrophoresis ◽  
Female Rats ◽  
Male Rats ◽  
Adult Rats ◽  
Male And Female ◽  
Glucose Levels ◽  
Male And Female Rats ◽  
Tsh Levels

ABSTRACT Male and female rats of varying ages were placad on a low iodine diet (LID) plus KClO4 or 6-propyl-2-thiouracil (PTU) or on the same diet supplemented with I (control rats). Goitrogenesis was also induced with LID plus PTU in gonadectomized animals of both sexes. The weight of the control and goitrogen treated animals, and the weight and iodine content of their thyroids were determined, as well as the plasma PBI, TSH, insulin and glucose levels. The pituitary GH-like protein content was assessed by disc electrophoresis on polyacrylamide gels. If goitrogenesis was induced in young rats of both sexes starting with rats of the same age, body weight (B.W.) and pituitary growth hormone (GH) content, it was found that both the males and females developed goitres of the same size. On the contrary, when goitrogenesis was induced in adult animals, it was found that male rats, that had larger B.W. and pituitary GH content than age-paired females, developed larger goitres. However, both male and female rats were in a hypothyroid condition of comparable degree as judged by the thyroidal iodine content and the plasma PBI and TSH levels. When all the data on the PTU or KClO4-treated male and female rats of varying age and B.W. were considered together, it was observed that the weights of the thyroids increased proportionally to B.W. However, a difference in the slope of the regression of the thyroid weight over B.W. was found between male and female rats, due to the fact that adult male rats develop larger goitres than female animals. In addition, in the male rats treated with PTU, gonadectomy decreased the B.W., pituitary content of GH-like protein and, concomitantly, the size of the goitre decreased; an opposite effect was induced by ovariectomy on the female animals. However, when goitrogenesis was induced in weight-paired adult rats of both sexes, the male animals still developed larger goitres than the females. Among all the parameters studied here, the only ones which appeared to bear a consistent relationship with the size of the goitres in rats of different sexes, treated with a given goitrogen, were the rate of body growth and the amount of a pituitary GH-like protein found before the onset of the goitrogen treatment. Moreover, though the pituitary content of the GH-like protein decreased as a consequence of goitrogen treatment, it was still somewhat higher in male that in female animals. The present results suggest that GH may somehow be involved in the mechanism by which male and female rats on goitrogens develop goitres of different sizes, despite equally high plasma TSH levels.

OXYDATION DES ANABOLIKUMS 1-METHYL-ANDROST-1-EN-17β-OL-3-ON MIT WASSERSTOFFTRANSFER AUF ÖSTRON

1971 ◽  
Vol 67 (3) ◽  
pp. 517-530 ◽  
Author(s):  
Martin Wenzel
Keyword(s):  
Rat Liver ◽  
Anabolic Steroid ◽  
Female Rats ◽  
Male Rats ◽  
Female Rat ◽  
Liver Slices ◽  
Androgen Effects ◽  
Biochemical Difference

ABSTRACT With the aid of metenolon-17α-T a tritium-transfer to oestrone in rat liver slices was demonstrated. This tritium-transfer from metenolon17α-T to oestrone yielding tritium-labelled oestradiol had a higher efficiency in male than in female rat liver. Correspondingly in the presence of metenolon the relation of oestrone to oestradiol is changed more in male than in female rat liver. Looking for biochemical differences between the anabolic steroid metenolon and testosterone the oxydation at C17 was measured in different organs of the rat using 17α-T-labelled steroids. The highest oxydation rate was found for both steroids in the liver. In the sexual organs of male rats the oxydation rate of testosterone was 50–10 times higher than that of the anabolic steroid. This difference was less in sexual organs of female rats. This result of a greater biochemical difference between both steroids in males than in females leads to the question, whether the dissociation between the anabolic and the androgen effects is higher in males than in females.

DYNAMICS OF BARLEY FLAVONOID AND PRODUCTIVITY UNDER UF-A RADIATION

1970 ◽  
pp. 14-18
Author(s):  
L. I. Goncharova ◽  
P. N. Tsygvintsev ◽  
О. А. Guseva
Keyword(s):  
Grain Yield ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Malonic Dialdehyde ◽  
Growth Period ◽  
Negative Effects ◽  
Uv Spectrum ◽  
Quantum Energy ◽  
Dry Soil ◽  
Universal Mechanism

The effect of increased UV-A radiation during the ontogeny of barley plants of the Vladimir variety in the vegetation experiment was studied. Changes in the content of malonic dialdehyde, flavonoids and grain yield were revealed. UV-A radiation as compared to UV-B radiation, has lower quantum energy and can have both positive and negative effects on plant regulatory and photosynthetic processes. One of the most damaging effects of increased levels of UV-A radiation is oxidative stress, which causes lipid peroxidation of biological membranes. The existence of a plant cell in such conditions is possible only thanks to a system of antioxidant defense mechanisms. The accumulation of phenolic compounds under the action of UV radiation is a universal mechanism of protection against photodamage, which was formed in the early stages of the evolution of photoautotrophic organisms. Flavonoids are localized in the epidermis of plant tissues and act as an internal filter. The content of flavonoids is determined by the genotype and due to ontogenetic patterns. Plants were grown in a greenhouse, in vessels containing 4.5 kg of air-dry soil. The repetition is threefold (3 vessels in each variant). Sowing density - 13 plants in each vessel. As a source of UV-A radiation used lamps Black Light BLUE company Philips. Plants were irradiated for 5 hours a day from 10 to 15 hours at 13, 25, 34, 43 and 52 stages of organogenesis. The magnitude of the daily biologically effective dose of UV-A radiation was 60.7 kJ / m2. The solar part of the UV spectrum in the vegetation experiment was absent in the greenhouse. The nature of changes in the content of flavonoids under the action of UV-A irradiation during the growing season of plants with the dynamics of the oxidative process has been established. The first maximum was observed during the vegetative growth period, the second - at the earing stage. The data obtained indicate that flavonoids have ontogenetic conditionality and perform photoprotective functions. The increase in their content under the action of UV-A radiation is accompanied by an increase in resistance to photodamage, which is confirmed by the formation of grain yield.

The effect of dexamethasone on mucosal immunity of uterine tissue during pregnancy in rat

2019 ◽  
Vol 20 (1) ◽  
pp. 75-84
Keyword(s):  
Early Pregnancy ◽  
Histopathological Examination ◽  
Early Period ◽  
Interleukin 10 ◽  
Treated Group ◽  
Female Rats ◽  
Male Rats ◽  
Mrna Levels ◽  
Uterine Tissue ◽  
Leukocytic Infiltration

Disturbances in early pregnancy immunity affect embryo development, endometrial receptivity, placental development, fetal growth and lead to subfertility, dexamethasone is a synthetic glucocorticoid used for treatment of various complications. Immune cells and cytokines were examined during the early pregnancy in twenty-four female rats and six male rats for mating. Rats were grouped into two group control and dexamethasone treated by a dose of 50µgm/kgm body weight daily starting from one week before mating and persisted for one week after pregnancy. Blood samples were collected from each rat at 5hrs and at 1,3,7 day of pregnancy. Extracted RNA was subjected to real time PCR to determine mRNA levels for immune related genes interleukin1a(IL1A) and interleukin 10(IL10). Histopathological examination was done to uterus in order to detect leukocyte infiltration in uterine tissue. Results showed that significant increase in white blood cell count mainly eosinophil at 5hrs and lymphocyte at three and seven day of pregnancy of dexamethasone treated group. Moreover, TNF, C-reactive protein and progesterone were increased mainly at seven day of pregnancy of dexamethasone treated group. Similarly, interleukin 1alpha and interleukin 10 significantly increased at 5hrs and one day of pregnancy of dexamethasone treated group. In contrast, serum levels of total antioxidant capacity and estrogen were decreased significantly at 5hrs and seven day in dexamethasone treated group. Histopathological examination of uterus revealed leukocytic infiltration especially neutrophil and few eosinophils at five hours and one day of gestation then eosinophil become absent at 3day and seven day of dexamethasone group. Epithelial height and uterine gland diameter significantly increased at 5hrs, three day and seven days of gestation of dexamethasone treated group. The present investigation demonstrated that using of dexamethasone by dose of 50µgm/kgm during early pregnancy had a conflicting impact on some immune cytokines and parameters and may reflect a harmful response of immune system toward early period of pregnancy

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