Liver Fat and Protein Metabolism in Rainbow Trout (Salmo gairdneri) Fed Cyclopropenoid Fatty Acids

1974 ◽  
Vol 31 (6) ◽  
pp. 1093-1100 ◽  
Author(s):  
Y. Malevski ◽  
M. W. Montgomery ◽  
R. O. Sinnhuber
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Rainbow Trout ◽  
Protein Metabolism ◽  
Body Weight Gain ◽  
Liver Weight ◽  
Liver Fat ◽  
Salmo Gairdneri ◽  
Acetate Incorporation ◽  
Cyclopropenoid Fatty Acid

Effects of cyclopropenoid fatty acid (CPFA) ingestion on fat and protein metabolism in the liver of rainbow trout (Salmo gairdneri) were studied. Three dietary regimens were used: experimental group fed 0.5 mg CPFA/kg body weight per day, pair-fed control, and ad lib. control. CPFA ingestion did not cause a reduction in body weight gain; however, the ratio of liver weight to body weight was elevated. Livers of CPFA-fed trout had higher fat and lower protein levels. Incorporation of 14C labelled amino acids into livers was reduced in CPFA-fed trout. Of the amino acids incorporated into the livers, a higher proportion was found in the liver proteins of CPFA-fed trout than pair-fed controls. The percentage of 14C labelled acetate incorporation into liver fat of CPFA-fed trout was similar to that of the pair-fed controls, whereas acetate incorporation into liver proteins was reduced. Activities of glutamate dehydrogenase (EC 1.4.1.3) and alanine aminotransferase (EC 2.6.1.2) were lower in CPFA-fed trout after 36 days on the diet; acetyl-CoA carboxylase (EC 6.4.1.2) activity was unaffected by the treatment.

Digestive Response of Rainbow Trout, Salmo gairdneri, to Pellet Diets

1969 ◽  
Vol 26 (7) ◽  
pp. 1801-1812 ◽  
Author(s):  
John T. Windell ◽  
David O. Norris ◽  
James F. Kitchell ◽  
James S. Norris
Keyword(s):  
Body Weight ◽  
Rainbow Trout ◽  
Dry Matter ◽  
Laboratory Experiments ◽  
Corn Oil ◽  
Saturated Fat ◽  
Methyl Cellulose ◽  
Salmo Gairdneri ◽  
Gastric Evacuation ◽  
Gastric Contents

Quantitative data are presented for three laboratory experiments concerning rate of gastric evacuation of pellets (fed in gelatin capsules) and pellet components. Rainbow trout, Salmo gairdneri, acclimated to a constant water temperature of 15 C were killed 12 hr after consuming an experimental meal. Subtraction of amount of dry matter remaining at autopsy from dry matter consumed yielded amount of food digested or evacuated or both, from the stomach per unit time.Meals of encapsulated pellet were evacuated from the stomach more rapidly (65.8% decrease in 12 hr) than encapsulated corn oil (42.6%), gelatin (50.8%), a combination of corn oil and gelatin (47.3%), saturated fat (28.8%), or methyl cellulose (50.3%).Groups of fish consuming five capsules equal to approximately 0.65 % of their body weight evacuated 35.9, 45.1, 64.2, 95.5, and 100% at intervals after killing from 6 to 36 hr, respectively. Similar groups consuming seven capsules equal to approximately 1.0% of their body weight evacuated 23.7, 57.9, 70.5, and 86.6% at intervals after killing from 4 to 20 hr, respectively.Ten groups of trout consuming amounts of dry matter equal to 0.24, 0.40, 0.74, 1.11, 1.31, 1.19, 1.59, 1.56, 1.91, and 2.26% of their body weight evacuated 90.7, 81.3, 64.2, 57.9, 56.6, 52.5, 53.4, 51.3, 58.7, and 50.0% in 12 hr, respectively. Gastric motility remains relatively constant once food has entered the stomach. However, when larger meals are fed a greater quantity is evacuated per unit time. This could only be accomplished by changes in volume of gastric contents pumped per peristaltic stroke or number of strokes per unit time.

scholarly journals Digestible methionine+cystine levels for white-egg layers aged one to six weeks

2020 ◽  
Vol 9 (8) ◽  
pp. e74985242
Author(s):  
Jalceyr Pessoa Figueiredo Junior ◽  
Fernando Guilherme Perazzo Costa ◽  
Ricardo Romão Guerra ◽  
Marcelo Helder Medeiros Santana ◽  
Matheus Ramalho de Lima ◽  
...  
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Liver Weight ◽  
Experimental Unit ◽  
Nutritional Requirements ◽  
Hepatic Glycogen ◽  
Gamma Glutamyl Transferase ◽  
Feed Conversion ◽  
Histological Data ◽  
Glutamyl Transferase

The aim of this study was was to determine the nutritional requirements of digestible methionine+cystine (M+C) for white-egg layers aged one to six weeks. A completely randomized design with five methionine+cystine levels, six replicates, and 30 birds per experimental unit was adopted. Dietary treatments consisted of five diets supplemented with DL-Methionine with resulted in five levels of digestible methionine + cystine, 80% (0.516%), 90% (0.578%), 100% (0.640%), 110% (0.702%), and 120% (0.764%), based on Brazilian tables of nutritional requirements. Performance, serological blood, and histological data were evaluated. Feed intake, feed conversion, hepatic glycogen deposition, enzymatic activity of alanine aminotransferase and gamma-glutamyl transferase, and serum creatinine and albumin levels had showed a quadratic response to the levels of digestible M+C, with the respective requirements: 89.78% (0.575%), 114.33% (0.732%), 86.50% (0.554%), 100% (0.640%), 100.40% (0.643%), 104.30% (0.668%), and 111.88% (0.716%). Increasing levels of methionine+cystine elevated the relative liver weight and the deposition of hepatic glycogen, in addition to promote higher growth in pullets, with better body weight and body weight gain and feed conversion ratio. Our findings suggest that 0.732% digestible methionine+cystine is recommended, which corresponds to an intake of 151.20 mg/bird/d and a Met+Cys:Lys  ratio 83%, for light pullets from one to six weeks.

scholarly journals A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome

2022 ◽  
Vol 8 ◽  
Author(s):  
Weilan Wang ◽  
Jodi E. Nettleton ◽  
Michael G. Gänzle ◽  
Raylene A. Reimer
Keyword(s):  
Body Weight ◽  
Body Fat ◽  
Body Weight Gain ◽  
Lactate Production ◽  
16S Rrna Gene Sequencing ◽  
Liver Weight ◽  
Rrna Gene ◽  
Metagenomic Sequencing ◽  
Microbial Composition ◽  
Maternal Consumption

To identify possible mechanisms by which maternal consumption of non-nutritive sweeteners increases obesity risk in offspring, we reconstructed the major alterations in the cecal microbiome of 3-week-old offspring of obese dams consuming high fat/sucrose (HFS) diet with or without aspartame (5–7 mg/kg/day) or stevia (2–3 mg/kg/day) by shotgun metagenomic sequencing (n = 36). High throughput 16S rRNA gene sequencing (n = 105) was performed for dams, 3- and 18-week-old offspring. Maternal consumption of sweeteners altered cecal microbial composition and metabolism of propionate/lactate in their offspring. Offspring daily body weight gain, liver weight and body fat were positively correlated to the relative abundance of key microbes and enzymes involved in succinate/propionate production while negatively correlated to that of lactose degradation and lactate production. The altered propionate/lactate production in the cecum of weanlings from aspartame and stevia consuming dams implicates an altered ratio of dietary carbohydrate digestion, mainly lactose, in the small intestine vs. microbial fermentation in the large intestine. The reconstructed microbiome alterations could explain increased offspring body weight and body fat. This study demonstrates that intense sweet tastants have a lasting and intergenerational effect on gut microbiota, microbial metabolites and host health.

scholarly journals Molecular Markers of Dietary Essential Amino Acid-deficiency (P08-059-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Joanna Moro ◽  
Nadezda Khodorova ◽  
Catherine Chaumontet ◽  
Patrick Even ◽  
Anne Blais ◽  
...  
Keyword(s):  
Body Weight ◽  
Molecular Markers ◽  
Lipid Metabolism ◽  
Protein Metabolism ◽  
Essential Amino Acid ◽  
Body Weight Gain ◽  
Mineral Density ◽  
Protein Sources ◽  
Growing Rats

Abstract Objectives The quality of dietary protein sources became a particularly sensitive issue in the current debates on a rebalancing between animal and vegetable food sources. The ability of a protein to meet the nutritional requirements of essential amino acid (EAA) is the basis for assessing the quality of protein. The objective of this study was to characterize the impact of lysine- and threonine-deficient gluten-based diets on the metabolism of growing rats and to identify molecular markers of these diets. Methods Growing rats were fed for 3 weeks with a threonine-supplemented and 70% lysine-deficient gluten diet; a lysine-supplemented and 47% threonine-deficient gluten diet; a gluten diet supplemented in lysine and threonine to meet all the AA requirements, and a control diet with milk protein to meet all the AA requirements. Body weight and food intake were measured daily. At the end of the experiment, tissues and biological fluids were removed. The body composition was analyzed, gene expression measurements involved in protein and lipid metabolism were performed and the urinary metabolome was analyzed by LC-MS. Statistical analysis was done by variance analysis and metabolome analysis by discriminant analysis of independent components. Results These EAA deficiency does not modify the food intake. Lysine deficiency induces a decrease in body weight gain, and lean body mass, associated with an increased in proteolysis and a decreased in proteosynthesis, a decreased in bone mineral density, and no effect on lipid metabolism. Threonine deficiency induces a decrease in body weight gain, and liver and skin weight, without changes in protein metabolism, bone mineral density, and lipid metabolism. After approval of the deficiency model, the metabolomic analysis performed on urine samples revealed the presence of specific discriminating molecules of the diets and types of proteins. Conclusions EAA deficiency has an impact on the growth, and bone and protein metabolism of growing rats. These deficiency states have resulted in different metabolome profiles that could lead to the identification of specific molecular markers of protein sources and related to EAA deficiencies. Funding Sources This study was funded by the UMR Nutrition Physiology and Ingestive Behavior. Supporting Tables, Images and/or Graphs

Lethal toxicity of permethrin (NRDC-143) to rainbow trout, salmo gairdneri, in relation to body weight and water temperature

1981 ◽  
Vol 15 (4) ◽  
pp. 503-505 ◽  
Author(s):  
A.K. Kumaraguru ◽  
F.W.H. Beamish
Keyword(s):  
Body Weight ◽  
Rainbow Trout ◽  
Water Temperature ◽  
Salmo Gairdneri ◽  
Lethal Toxicity

scholarly journals Molecular markers of dietary essential amino acid-deficiency

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Joanna Moro ◽  
Nadezda Khodorova ◽  
Catherine Chaumontet ◽  
Patrick Even ◽  
Anne Blais ◽  
...  
Keyword(s):  
Body Weight ◽  
Molecular Markers ◽  
Lipid Metabolism ◽  
Protein Metabolism ◽  
Essential Amino Acid ◽  
Body Weight Gain ◽  
Mineral Density ◽  
Protein Sources ◽  
Growing Rats

AbstractIntroduction:The quality of dietary protein sources became a particularly sensitive issue in the current debates on a rebalancing between animal and vegetable food sources.The ability of a protein to meet the nutritional requirements of essential amino acid (EAA) is the basis for assessing the quality of protein.The objective of this study was to characterize the impact of lysine- and threonine-deficient gluten-based diets on the metabolism of growing rats and to identify molecular markers of these diets.Materials and Methods:Growing rats were fed for 3 weeks with a threonine-supplemented and 70% lysine-deficient gluten diet; a lysine-supplemented and 47% threonine-deficient gluten diet; a gluten diet supplemented in lysine and threonine to meet all the AA requirements, and a control diet with milk protein to meet all the AA requirements.Body weight and food intake were measured daily. At the end of the experiment, tissues and biological fluids were removed. The body composition was analyzed, gene expression measurements involved in protein and lipid metabolism were performed and the urinary metabolome was analyzed by LC-MS. Statistical analysis was done by variance analysis and metabolome analysis by discriminant analysis of independent components.Results:This EAA deficiency does not modify the food intake. Lysine deficiency induces a decrease in body weight gain, and lean body mass, associated with an increased in proteolysis and a decreased in proteosynthesis, a decreased in bone mineral density, and no effect on lipid metabolism.Threonine deficiency induces a decrease in body weight gain, and liver and skin weight, without changes in protein metabolism, bone mineral density, and lipid metabolism. After approval of the deficiency model, the metabolomic analysis performed on urine samples revealed the presence of specific discriminating molecules of the diets and types of proteins.Discussion:EAA deficiency has an impact on the growth, and bone and protein metabolism of growing rats. These deficiency states have resulted in different metabolome profiles that could lead to the identification of specific molecular markers of protein sources and related to EAA deficiencies.

Models of Ammonia Excretion for Brook Trout (Salvelinus fontinalis) and Rainbow Trout (Salmo gairdneri)

1980 ◽  
Vol 37 (9) ◽  
pp. 1421-1425 ◽  
Author(s):  
Larry J. Paulson
Keyword(s):  
Body Weight ◽  
Rainbow Trout ◽  
Brook Trout ◽  
Salvelinus Fontinalis ◽  
Ammonia Excretion ◽  
Salmo Gairdneri ◽  
Feeding Rates ◽  
Data Set ◽  
Nitrogen Consumption ◽  
Good Agreement

Ammonia excretion by brook trout (Salvelinus fontinalis) and rainbow trout (Salmo gairdneri) was measured in relation to nitrogen consumption, body weight (15–154 g for rainbow trout and 50–360 g for brook trout), and temperature (11.2–21.0 °C) under laboratory conditions. Four natural diets, collected from Castle Lake, California, and a commercial pellet diet were fed to the trout in gelatin capsules at feeding rates from 2.5 to 5% body weight∙d−1. Nitrogen consumption was the most important factor influencing ammonia excretion, followed by body weight and temperature. Testing the models with an independent data set revealed good agreement between measured and predicted rates of excretion. The models seem to estimate adequately ammonia excretion by trout in both natural and artificial aquatic systems.Key words: models, ammonia excretion, nitrogen consumption, body weight, temperature, multiple regression, rainbow trout, brook trout

Subchronic Toxicity of Monensin in Broiler Chickens

1983 ◽  
Vol 20 (3) ◽  
pp. 353-359 ◽  
Author(s):  
D. D. Wagner ◽  
R. D. Furrow ◽  
B. D. Bradley
Keyword(s):  
Body Weight ◽  
Feed Efficiency ◽  
Broiler Chickens ◽  
Continuous Monitoring ◽  
Body Weight Gain ◽  
Liver Weight ◽  
Broiler Chicks ◽  
Subchronic Toxicity ◽  
Toxic Dose ◽  
Growing Period

One hundred ninety-two male broiler chicks were dosed with monensin at concentrations of 0, 121, or 242 mg/kg feed throughout the normal growing period (50 days). Body weight gain and feed efficiency were determined weekly, and cardiac muscle was examined grossly and histologically at the end of the experiment. Livers also were weighed and examined grossly. Feed intake was determined daily, allowing continuous monitoring of drug intake. No depressing effects of the drug on growth rate and efficiency were observed until after four weeks, and then were evident only in the chicks receiving the 242 mg/kg diet. Subepicardial hemorrhage and congestion occurred in 40% of the hearts from the chickens fed the high monensin dose and were nonexistent in the other treatments. There appeared to be an inverse relationship between monensin dose and liver weight. The paralytic effects previously reported from acute dosing experiments were not observed. The results show that the heart and probably the liver are sensitive indicators of monensin toxicity and that the subchronic toxic dose is less than 18 mg/kg body weight per day.

A Study of in vivo Glycine Absorption by Fed and Fasted Rainbow Trout (Salmo Gairdneri)

1981 ◽  
Vol 91 (1) ◽  
pp. 285-292 ◽  
Author(s):  
G. BOGÉ ◽  
A. RIGAL ◽  
G. PÉRES
Keyword(s):  
Body Weight ◽  
Rainbow Trout ◽  
Weight Gain ◽  
Amino Acid ◽  
Acid Concentration ◽  
Dry Weight ◽  
Salmo Gairdneri ◽  
Mucosal Tissue ◽  
Perfusion Technique

The effects of 4 and 8 weeks fasting at 16 °C were studied in rainbow trout, Salmo gairdneri Richardson. After 4 and 8 weeks, the wet weights of the intestine of fasted animals are respectively 64% and 69% lower than those of fed animals. These effects especially concern the mucosal tissue. Glycine absorption (0.5 and 10 mm) was studied using an in vivo perfusion technique. After 4 weeks, the absolute amounts of 0.5 mm glycine absorbed by fasted and fed fish are similar. With 10 mm glycine, the absorption is slightly lower in fasted trout (−19%). After 8 weeks these differences are more marked, with glycine concentrations of 10 mm (−42%). Results expressed per 100 g body weight showed that these differences result partly from a weight gain of fed trout. Absorption expressed in terms of weight of dry intestine is higher in 4 and 8 weeks fasted animals, principally for the lower amino acid concentration (+61% and +111%). Larger differences were apparent when the absorptions were expressed in terms of dry weight of mucosal tissue (+122% and +225%).

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