7-O-β-D-Glucosyl-3′,4′,5-trihydroxy-6-methyl flavanone—a new C-methyl flavanone glycoside from Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] roots

1969 ◽  
Vol 47 (5) ◽  
pp. 869-871 ◽  
Author(s):  
G. M. Barton
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Pseudotsuga Menziesii ◽  
Douglas Fir ◽  
High Yield ◽  
Root Bark ◽  
Physical Tests ◽  
Flavanone Glycoside

A new C-methyl flavanone, 7-O-β-D-glucosyl-3′,4′,5-trihydroxy-6-methyl flavanone, has been obtained from healthy Douglas-fir [Pseudotsugamenziesii (Mirb.) Franco] root bark in a high yield of 2.6%. Its structure was elucidated by high resolution and mass spectrometry together with a variety of chemical and physical tests and confirmed by synthesis of its methylated aglycone. Pathological testing of this new glucoside against Poriaweirii Murr. is being undertaken.

scholarly journals Homocylindrocarpidin und 17-Demethoxy-cylindrocarpidin, zwei neue Alkaloide aus Tabernaemontana amygdali folia

1967 ◽  
Vol 22 (9) ◽  
pp. 955-957 ◽  
Author(s):  
Hans Achenbach
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Spectral Data ◽  
High Resolution Mass Spectrometry ◽  
Root Bark ◽  
High Resolution Mass ◽  
Resolution Mass

Chromatography of the extract of the root bark of Tabernaemontana amygdalifolia yielded two dihydroindole alkaloids. On the basis of spectral data, mainly conventional and high-resolution mass spectrometry, they were shown to be Homocylindrocarpidine (I) and 17-Demethoxy-cylindrocarpidine (II), respectively.

Variation in the root bark phenolics/sugar ratio of Douglas-fir grown in two plantations in northern Idaho

2002 ◽  
Vol 32 (3) ◽  
pp. 556-560 ◽  
Author(s):  
Jennifer H Myszewski ◽  
Lauren Fins ◽  
James A Moore ◽  
Marc Rust ◽  
Peter G Mika
Keyword(s):  
Environmental Factors ◽  
Pseudotsuga Menziesii ◽  
Height Growth ◽  
Douglas Fir ◽  
Significant Source ◽  
Root Bark ◽  
Bark Tissue ◽  
Bark Chemistry ◽  
Root Chemistry ◽  
Northern Idaho

Several studies have linked high phenolics/sugar ratios in the inner root bark tissue of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) to decreased susceptibility to Armillaria spp. While these studies have identified environmental factors that influence root chemistry, none have examined whether the phenolics/sugar ratio is genetically controlled. In this study, we investigated the effects of genetics and environment on the root bark chemistry of 20 families of 15-year-old Douglas-fir planted in two sites in northern Idaho. Only sugar concentrations varied significantly among families, but site was a significant source of variation for phenolics and the phenolics/sugar ratio. Family × site interactions were significant for the concentrations of all measured root bark compounds as well as for the phenolics/sugar ratio. Phenotypic correlations between height and the phenolics/sugar ratio and between height and sugar concentrations were not significant. However, families with superior height growth and below-average sugar concentrations could be found at both sites. Should a high phenolics/sugar ratio prove effective in selecting genotypes for resistance to Armillaria infection, these results suggest that gains could be made more efficiently by selecting for low sugar concentrations.

Exploring the Active Compounds of Traditional Mongolian Medicine Agsirga in Intervention of Novel Coronavirus (2019-nCoV) Based on HPLC-Q-Exactive-MS/MS and Molecular Docking Method

2020 ◽  
Author(s):  
Jie Cheng ◽  
Yuchen Tang ◽  
Baoquan Bao ◽  
Ping Zhang
Keyword(s):  
Mass Spectrometry ◽  
Molecular Docking ◽  
High Resolution ◽  
Mass Spectra ◽  
High Resolution Mass Spectrometry ◽  
Structural Formula ◽  
Active Compounds ◽  
High Resolution Mass ◽  
Q Exactive ◽  
Resolution Mass

<p><a></a><a></a><a></a><a><b>Objective</b></a>: To screen all compounds of Agsirga based on the HPLC-Q-Exactive high-resolution mass spectrometry and find potential inhibitors that can respond to 2019-nCoV from active compounds of Agsirga by molecular docking technology.</p> <p><b>Methods</b>: HPLC-Q-Exactive high-resolution mass spectrometry was adopted to identify the complex components of Mongolian medicine Agsirga, and separated by the high-resolution mass spectrometry Q-Exactive detector. Then the Orbitrap detector was used in tandem high-resolution mass spectrometry, and the related molecular and structural formula were found by using the chemsipider database and related literature, combined with precise molecular formulas (errors ≤ 5 × 10<sup>−6</sup>) , retention time, primary mass spectra, and secondary mass spectra information, The fragmentation regularities of mass spectra of these compounds were deduced. Taking ACE2 as the receptor and deduced compounds as the ligand, all of them were pretreated by discover studio, autodock and Chem3D. The molecular docking between the active ingredients and the target protein was studied by using AutoDock molecular docking software. The interaction between ligand and receptor is applied to provide a choice for screening anti-2019-nCoV drugs.</p> <p><b>Result</b>: Based on the fragmentation patterns of the reference compounds and consulting literature, a total of 96 major alkaloids and stilbenes were screened and identified in Agsirga by the HPLC-Q-Exactive-MS/MS method. Combining with molecular docking, a conclusion was got that there are potential active substances in Mongolian medicine Agsirga which can block the binding of ACE2 and 2019-nCoV at the molecular level.</p>

Analysis of Squalene and Its Transformation By-Products in Latent Fingermarks by Ultrahigh-Pressure Liquid Chromatography-High Resolution Accurate Mass Orbitrap™ Mass Spectrometry

2019 ◽  
Author(s):  
Buddhika Dorakumbura ◽  
Francesco Busetti ◽  
Simon Lewis
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Storage Conditions ◽  
Ultrahigh Pressure ◽  
Accurate Mass ◽  
Rational Approach ◽  
By Products ◽  
Over Time ◽  
High Resolution Accurate Mass

<p>Transformation of squalene and its by-products in fingermarks over time under different storage conditions (light, dark and underwater) was examined through ultrahigh-pressure liquid chromatography high resolution accurate mass Orbitrap™ mass spectrometry. Complications of assessing fingermark compositional variation over time using multiple samples with varying initial compositions were elucidated and a more rational approach was successfully demonstrated. Squalene was detected in all fresh natural fingermarks and the amount ranged between 0.20 to 11.32 μg/5 fingertips. A notable difference in the transformation of squalene was observed with different storage conditions, where a dark aquatic environment accelerated degradation of squalene compared to dark but dry conditions. Squalene monohydroperoxide was extremely short-lived in natural deposits while the amount of squalene epoxide was still increasing relative to the initial amount, after ageing under dark and aquatic conditions for up to 7 days. Some oxidation by-products of cholesterol were also tentatively identified, which exhibited a growth over time against their initial concentration under any of the storage condition tested. These by-products, therefore, show potential as biomarkers for targeted visualisation of aged deposits.</p>

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

Sign in / Sign up

Export Citation Format

Share Document