Carbon Tetrachloride-Induced Hepatotoxicity: Protective Effect of 'Rocket' Eruca sativa L. in Rats

2010 ◽  
Vol 38 (01) ◽  
pp. 75-88 ◽  
Author(s):  
Saleh Alqasoumi
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Liver Tissue ◽  
Hepatic Injury ◽  
Liver Toxicity ◽  
Elevated Serum ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Eruca Sativa ◽  
Protein Sulfhydryl

The hepatoprotective and antioxidant effect of an ethanolic extract of 'Rocket' Eruca sativa L. (EER), on liver injury induced by carbon tetrachloride ( CCl4 ) was investigated. Wistar albino rats were administered 250 and 500 mg/kg body weight extract orally for 10 consecutive days. Marker enzymes GOT, GPT, ALP, GGT and bilirubin were estimated in serum. Whereas, non-protein sulfhydryl (NP-SH), total protein (TP) and malondialdehyde (MDA) were estimated in liver tissue as markers for oxidative stress. Histopathological assessment was also done on liver tissue. CCl4 induced liver poisoning in all treated animals was evident by elevated serum GOT, GPT, ALP, GGT and bilirubin levels. Induction of oxidative stress in the liver tissue by CCl4 was evidenced by a fall in the levels of NP-SH and TP; and an increased level of MDA concentration. EER administration for 10 days prevented the CCl4 induced hepatic injury and oxidative stress. Furthermore, the extract also reduced the pentobarbital-induced prolongation of sleeping time in mice. The ability of rocket extract to protect the liver toxicity in rats was further confirmed by histological findings in the liver tissue. In conclusion, it was observed that Eruca sativa L. extract protects the liver against CCl4 induced hepatic injury through its potent antioxidant activity in rats.

scholarly journals Effect of Grape Seed Oil on Chronic Carbon Tetrachloride-Induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats

2019 ◽  
pp. 1-10
Author(s):  
Ayhan Atasever ◽  
Ahmet Alpay ◽  
Gorkem Ekebas ◽  
Duygu Yaman Gram
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Caspase 3 ◽  
Seed Oil ◽  
Antioxidant Properties ◽  
Elevated Serum ◽  
Grape Seed ◽  
Control Group ◽  
Albino Rats ◽  
Grape Seed Oil

Aims: The present study was designed to evaluate the hepatoprotective activity of grape seed oil (GSO) on liver lesions induced by carbon tetrachloride (CCl4) in rats. The effects on hepatic injury were investigated by measuring serum levels of ALT, triglyceride, total protein, total cholesterol and liver levels of MDA. Furthermore, caspase -3, -8 and -9 activities in cellular apoptosis were determined. Place and Duration of Study: Faculty of Veterinary Medicine, Department of Pathology, Erciyes University, Kayseri, between November 2017 and September 2018. Methodology: In this study 40 male Wistar albino rats were divided into four groups including 10 animals in each. Control group administered with 0.9% NaCl. The second group was administered with 4 mL/kg GSO for twelve weeks. Third group were given CCl4 (0.2 mL/kg) twice for 8-weeks. Fourth group was administered with 4 mL/kg GSO, for 12 weeks and also given CCl4 (0.2 mL/kg) twice for 8 weeks, starting from the 5th week. Results: Histopathological examination of CCl4 group showed intense macro and micro vesicular steatosis in hepatocytes, necrosis, and lymphocytes rich mononuclear cell infiltration in portal area and mild portal fibrosis in the parenchyma. The grape seed oil applications have partially normalized the altered histological changes and the activity of caspase -3, -8 and -9. Administration of GSO led to a decline in the activities of ALT and MDA levels while this treatment elevated serum triglycerides levels which are not significantly important. Conclusion: The results indicate that the antioxidant properties of GSO have not ameliorative effect in either the histopathological lesions or biochemical parameters against CCl4-induced hepatotoxicity in rats. Also, it has been concluded that duration‐dependent further research results are needed to determine the effects of grape seed oil in high doses which can give the best results without side effects.

scholarly journals Pien Tze Huang Gan Bao ameliorates carbon tetrachloride-induced hepatic injury, oxidative stress and inflammation in rats

2017 ◽  
Vol 13 (5) ◽  
pp. 1820-1826 ◽  
Author(s):  
Jinyan Zhao ◽  
Haixia Hu ◽  
Yun Wan ◽  
Yuchen Zhang ◽  
Liangpu Zheng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Pien Tze Huang

scholarly journals Enhancement of Liver Toxicity on Diabetes Mellitus by a Universal Chemical Pollutant (Acrylonitrile) in Rats

2019 ◽  
Vol 26 (2) ◽  
pp. 9-17
Author(s):  
Sameer E. Alharthi
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Aqueous Solution ◽  
Liver Toxicity ◽  
Elevated Serum ◽  
Diabetic Rat ◽  
Control Group ◽  
Distilled Water ◽  
Serum Aminotransferase ◽  
Cyp2e1 Activity

The present study was designed to investigate potential liver damage due to acrylonitrile in Streptozotocin induced diabetes in rats. Twenty-four rats were divided into 4 treatment groups. Nondiabetic control rat receiving distilled water, non-diabetic rat receiving acrylonitrile aqueous solution (10 mg/kg/day), diabetic control rat receiving distilled water and diabetic rat receiving acrylonitrile aqueous solution. All groups received the treatment for 4 weeks. The animals were assessed for hepatoxicity markers in serum, oxidative stress markers, CYP2E1 activity and cyanide formation in tissues. Acrylonitrile significantly elevated serum aminotransferase, alanine aminotransferase, total bilirubin levels, triglycerides and total cholesterol in diabetic groups as compared to normal control group. Antioxidant markers like glutathione showed significant decline while a significant increase in malondialdehyde, superoxide dismutase and catalase in diabetic rats treated with acrylonitrile. CYP2E1 activity was observed in acrylonitrile – exposed nondiabetic and diabetic groups as compared to control. Cyanide formation was raised in both the nondiabetic and diabetic groups as compared to control group. Acrylonitriles can produce acute hepatic injury, induction of diabetes mellitus type II, and accomplish the CYP2E1 enzyme which sequentially leads to generation of oxidative stress and its metabolic product–cyanide that may potentiate the oxidative stress posing more deleterious effect.

scholarly journals Emblica officinalis (Amla) Ameliorates High-Fat Diet Induced Alteration of Cardiovascular Pathophysiology

2019 ◽  
Vol 17 (1) ◽  
pp. 52-63 ◽  
Author(s):  
Bheemshetty S. Patil ◽  
Pallavi S. Kanthe ◽  
Chandramouli R. Reddy ◽  
Kusal K. Das
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Histopathological Study ◽  
Histopathological Analysis ◽  
Phytochemical Analysis ◽  
Emblica Officinalis ◽  
High Fat ◽  
Elastic Artery

Background: Dietary high fat possibly causes oxidative stress. Also, it alters the pathophysiology of metabolically active myocardial tissues and vascular architecture. Emblica officinalis contains a potential antioxidant that counteracts oxidative stress and possibly maintains vascular integrity. Objective: To assess the effect of ethanolic extract of Emblica officinalis (EEO) on High Fat Diet (HFD) induced changes in vascular chemistry and histopathology of the cardiovascular system in male albino rats. Materials and Methods: Ethanolic extract of Emblica Officinalis (EEO) was prepared and phytochemical analysis was done. Rats were divided into four groups, having six rats in each group as follows: group 1- Control (20% fat); group 2 (20% fat+ EEO 100 mg/kg/b w); group 3 (30% fat) and group 4 (30% fat + EEO 100 mg/kg/b w). Dietary and EEO supplementation was continued for 21 days. Gravimetric and oxidative stress markers like MDA, NO, antioxidants like Vitamin C and E, and molecular marker (NOS3) were evaluated. Histopathological analysis was done on the myocardium and elastic artery along with measurement of coronary arterial wall thickness and lumen diameter. One way ANOVA was done for analysis of data. Results: High fat diet showed a significant increase in MDA, decrease of NO with unaltered NOS3 protein in rats fed with high fat diet, which indicate possible alteration of vascular pathophysiology. Supplementation of EEO showed an ameliorating effect on high fat diet induced oxidative stress. These results were further corroborated with findings of a histopathological study on the myocardium, elastic artery and coronary arterial architecture. Conclusion: Ethanolic extract of Emblica officinalis (EEO) indicates its cardioprotective efficacy against rats fed with high fat diet.

scholarly journals Melatonin synergistically enhances protective effect of atorvastatin against gentamicin-induced nephrotoxicity in rat kidney

2016 ◽  
Vol 94 (3) ◽  
pp. 265-271 ◽  
Author(s):  
Saeed Mehrzadi ◽  
Seyed Kamran Kamrava ◽  
Banafshe Dormanesh ◽  
Manijeh Motevalian ◽  
Azam Hosseinzadeh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Combination Therapy ◽  
Serum Creatinine ◽  
Rat Kidney ◽  
Elevated Serum ◽  
Albino Rats ◽  
Kidney Weight ◽  
Sod Activity ◽  
Creatinine Concentration ◽  
Beneficial Effects

The risk of serious side-effects such as nephrotoxicity is the principal limitation of gentamicin (GEN) therapeutic efficacy. Oxidative stress is considered to be an important mediator of GEN-induced nephrotoxicity. The present study was designed to evaluate the efficacy of the combination of melatonin (MT) plus atorvastatin (ATO) against GEN-induced nephrotoxicity in rats. We utilized 30 male Wistar albino rats allocated in 5 groups, each containing 6 rats: control, GEN (100 mg/kg/day), ATO (10 mg/kg/day) + GEN, MT (20 mg/kg/day) + GEN, and ATO (10 mg/kg/day) plus MT (20 mg/kg/day) + GEN. Kidney weight, serum creatinine and urea concentration, renal ROS, MDA, GSH levels, SOD, and CAT activity were determined. GEN-induced nephrotoxicity was evidenced by marked elevations in serum urea and creatinine, kidney weight, renal ROS, and MDA levels and reduction in renal GSH level, SOD and CAT activity. MT pretreatment significantly lowered the elevated serum creatinine concentration, kidney weight, renal ROS and MDA levels. However ATO could not reduce these parameters, but similarly to MT, it was able to enhance the renal GSH level, CAT and SOD activity. In addition, a combination therapy of MT plus ATO enhanced the beneficial effects of ATO, while not changing the effects of MT effects or even improving them. The present study indicates that a combination therapy of MT plus ATO can attenuate renal injury in rats treated with GEN, possibly by reducing oxidative stress, and it seems that MT can enhance the beneficial effects of ATO.

scholarly journals Chlorogenic and Caftaric Acids in Liver Toxicity and Oxidative Stress Induced by Methamphetamine

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Khaled M. M. Koriem ◽  
Rowan E. Soliman
Keyword(s):  
Oxidative Stress ◽  
Chlorogenic Acid ◽  
Liver Toxicity ◽  
Density Lipoprotein ◽  
Acute Hepatic Failure ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Dietary Polyphenols ◽  
And Oxidative Stress

Methamphetamine intoxication can cause acute hepatic failure. Chlorogenic and caftaric acids are the major dietary polyphenols present in various foods. The aim of this study was to evaluate the protective role of chlorogenic and caftaric acids in liver toxicity and oxidative stress induced by methamphetamine in rats. Thirty-two male albino rats were divided into 4 equal groups. Group 1, which was control group, was injected (i.p) with saline (1 mL/kg) twice a day over seven-day period. Groups 2, 3, and 4 were injected (i.p) with methamphetamine (10 mg/kg) twice a day over seven-day period, where groups 3 and 4 were injected (i.p) with 60 mg/kg chlorogenic acid and 40 mg/kg caftaric acid, respectively, one day before methamphetamine injections. Methamphetamine increased serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, bilirubin, cholesterol, low-density lipoprotein, and triglycerides. Also, malondialdehyde in serum, liver, and brain and plasma and liver nitric oxide levels were increased while methamphetamine induced a significant decrease in serum total protein, albumin, globulin, albumin/globulin ratio, brain serotonin, norepinephrine and dopamine, blood and liver superoxide dismutase, and glutathione peroxidase levels. Chlorogenic and caftaric acids prior to methamphetamine injections restored all the above parameters to normal values. In conclusion, chlorogenic and caftaric acids before methamphetamine injections prevented liver toxicity and oxidative stress where chlorogenic acid was more effective.

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