Inhibition of Microsomal Lipid Peroxidation and Protein Oxidation by Carica papaya (L) Leaf against Carbon Tetrachloride-Induced Hepatic Injury in Wistar Albino Rats

INTRODUCTION: The present study evaluated the modulatory effects of diphenylhydrazine induced experimental wistar albino rats and also to assess various biochemical parameters in whole blood and red blood cell lysate.MATERIALAND METHODS: Twenty male albino rats weighing 180-200 gm were selected for the study and divided in two groups; ten phenylhydrazine dihydrochloride (PHZ) induced anemia and ten healthy control. Thiobarbituric acid reactive substances and lipid hydroperoxide were measured as lipid peroxidation parameter. The antioxidant vitamins A, C and E and enzymatic antioxidants; catalase, glutathione peroxidase and superoxide dismutase were also assessed.RESULTS: Phenylhydrazine induced anemic rats showed a significant increase in the lipid peroxidation and decrease in the antioxidants as compared to healthy rats.CONCLUSION: The study concludes that phenylhydrazine induced experimental anemic albino rats showed increased oxidative stress than compared with healthy albino rats.Journal of Universal College of Medical Sciences Vol. 3, No. 1, 2015: 41-47

Download Full-text

A composition of medicinal plants with an enhanced ability to suppress microsomal lipid peroxidation and a protective activity against carbon tetrachloride-induced hepatotoxicity

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2017.11.085 ◽

2017 ◽

Vol 96 ◽

pp. 1283-1291 ◽

Cited By ~ 2

Author(s):

Araylim N. Aralbaeva ◽

Aigul T. Mamataeva ◽

Nazgul I. Zhaparkulova ◽

Raisa S. Utegalieva ◽

Marina Khanin ◽

...

Keyword(s):

Lipid Peroxidation ◽

Carbon Tetrachloride ◽

Medicinal Plants ◽

Protective Activity ◽

Microsomal Lipid Peroxidation

Download Full-text

STANDARDIZATION OF MODEL OF INDUCTION OF HEPATOTOXICITY WITH ANTI-TUBERCULOSIS DRUGS IN WISTAR ALBINO RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.11971 ◽

2017 ◽

Vol 10 (6) ◽

pp. 150

Author(s):

Sarita M Kapgate ◽

Abhijit B Patil

Keyword(s):

Liver Injury ◽

Animal Studies ◽

Hepatic Injury ◽

Albino Rats ◽

Histopathological Changes ◽

First Line ◽

Drug Induced ◽

Significant Development ◽

Reported Study ◽

Wistar Albino Rats

Objective: The objective of the study to standardize the model of hepatotoxicity induced by ATT drugs in Wistar Albino rats. Isoniazid (INH), rifampicin (RMP), pyrazinamide (PZA), the first line drugs used in the treatment of tuberculosis (TB) associated with the potential adverse effect. Numerous animal studies were reported endeavoring induction and cure of anti-TB (ATT) drug-induced hepatotoxicity using herbal and chemical drugs. However, the previous reported study failed to replicate where Wistar albino rats were treated with INH, RMP, and PZA and had shown the significant development of liver injury. Hence in present paper, aimed to develop a standardize model of induction of hepatotoxicity with ATT drugs.Methods: Wistar rats were treated with ATT drugs in combination in various doses up to 4-8 weeks. Total nine experiments were conducted to achieve successful hepatotoxicity. The aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were the biochemical parameters of assessment. Histopathological changes in the liver were also examined.Results: No evidence of any liver injury or an inflammatory infiltrate has been observed as had been reported in the previous studies. Rather decrease in serum ALT levels has been observed by researcher. In short, hepatic injury cannot be developed with the doses used in previous reported papers. The successful attempt to induce hepatotoxicity can be achieved with the doses of INH - 100, RMP - 300, PZA - 700 mg/kg. The findings were confirmed by the raised ALT, AST, and ALP levels compared with baseline. The histopathological changes also support the findings.Conclusion: The dose of INH - 100, RMP – 300 and PZM - 700 mg/kg. Succeeds to induce hepatotoxicity in Wistar albino rats and Swiss albino mice as well.

Download Full-text

Protective effects of honey and bee venom against lipopolysaccharide and carbon tetrachloride-induced hepatoxicity and lipid peroxidation in rats

Toxicology Research ◽

10.1093/toxres/tfaa077 ◽

2020 ◽

Vol 9 (5) ◽

pp. 693-705

Author(s):

Noha M Meligi ◽

Suzan Alaa Ismail ◽

Nagy S Tawfik

Keyword(s):

Lipid Peroxidation ◽

Carbon Tetrachloride ◽

Hematological Parameters ◽

Lipid Peroxide ◽

Alpha Fetoprotein ◽

Bee Venom ◽

Albino Rats ◽

Average Weight ◽

Protective Effects ◽

Liver Histopathology

Abstract In the present study, the protective effects of honey and bee venom (BV) either independently or in combination against lipopolysaccharide (LPS) and carbon tetrachloride (CCl4)-induced hepatoxicity, lipid peroxidation, and hematological alterations in male albino rats were investigated. In addition, histopathological alterations of hepatic tissues induced by LPS/CCL4 were recorded. Sixty-four of male albino rats of average weight 120–150 g were included in this study. Rats were divided into eight equal groups of eight. The obtained results demonstrated that treatment with LPS/CCl4 caused an increase in the levels of alpha-fetoprotein, which was accompanied by changes in the hepatic function biomarkers that characterized by the increased levels of transaminases (AST, ALT). The results showed oxidative stress as assigned by the increase in lipid peroxide. Meantime detraction in the antioxidants, including glutathione peroxidase was observed. Interruptions in biochemical parameters accompanied by disturbances in hematological parameters and liver histopathology were resulted due to exposure to LPS/CCl4. This study showed the use of honey and BV provided a protective effect on hepatotoxicity induced by LPS/CCl4. This might have been occurred through the reduction of hepatic transaminases and the “Alpha-fetoprotein” in serum and the equilibration of the antioxidation system, thereby, inhibiting the reactive oxygen species accumulation. Honey and BV administration reestablish disturbed hematological parameters and liver histopathology persuaded by LPS/CCl4. More interesting, we demonstrated that using a combination of the honey and BV showed promising enhancement in their protective effects over the use of just one of the two reagents.

Download Full-text

Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue

Toxicology and Industrial Health ◽

10.1191/0748233704th208oa ◽

2004 ◽

Vol 20 (6-10) ◽

pp. 141-147 ◽

Cited By ~ 20

Author(s):

Alpaslan Terzi ◽

Mustafa Iraz ◽

Semsettin Sahin ◽

Atilla Ilhan ◽

Nuri Idiz ◽

...

Keyword(s):

Lipid Peroxidation ◽

Liver Tissue ◽

Antioxidant Properties ◽

Albino Rats ◽

Protective Agent ◽

Protective Effects ◽

Oxidant Injury ◽

Sod Activity ◽

Significant Difference ◽

Wistar Albino Rats

Rotenone, an insecticide of botanical origin, causes toxicity through inhibition of complex I of the respiratory chain in mitochondria. This study was undertaken to determine whether rotenone-induced liver oxidant injury is prevented by erdosteine, a mucolytic agent showing antioxidant properties. There were four groups of Male Wistar Albino rats: group one was untreated as control; the other groups were treated with erdosteine (50 mg/kg per day, orally), rotenone (2.5 mg/mL once and 1 mL/kg per day for 60 days, i.p.) or rotenone plus erdosteine, respectively. Rotenone treatment without erdosteine increased xanthine oxidase (XO) enzyme activity and also increased lipid peroxidation in liver tissue P < 0.05). The rats treated with rotenone plus erdosteine produced a significant decrease in lipid peroxidation and XO activities in comparison with rotenone group PB / 0.05). Erdosteine treatment with rotenone led to an increase in catalase (CAT) and superoxide dismutase (SOD) activities in comparison with the rotenone group PB / 0.05). There was no significant difference in nitric oxide (NO) level between groups. There were negative correlations between CAT activity and malondialdehyde (MDA) level (r= -0.934, P <0.05) with between CAT and SOD activities (r= -0.714, P <0.05), and a positive correlation between SOD activity and MDA level (r= 0.828, P <0.05) in rotenone group. In the rotenone plus erdosteine group, there was a negative correlation between XO activity and NO level in liver tissue (r= -0.833, P -0.05). In the light of these findings, erdosteine may be a protective agent for rotenone-induced liver oxidative injury in rats.

Download Full-text

Hepatoprotective Effect of Sarvakalp Kwath Against Carbon Tetrachloride Induced Hepatic Injury in Albino Rats

Biomedical & Pharmacology Journal ◽

10.13005/bpj/538 ◽

2014 ◽

Vol 7 (2) ◽

pp. 659-663 ◽

Cited By ~ 1

Author(s):

A. Yadav ◽

S. Kumar

Keyword(s):

Carbon Tetrachloride ◽

Hepatic Injury ◽

Hepatoprotective Effect ◽

Albino Rats

Download Full-text

Effect of dietary antioxidants and phenobarbital pretreatment on microsomal lipid peroxidation and activation by carbon tetrachloride

Life Sciences ◽

10.1016/0024-3205(76)90250-2 ◽

1976 ◽

Vol 19 (8) ◽

pp. 1151-1160 ◽

Cited By ~ 12

Author(s):

Steve L. Taylor ◽

Al L. Tappel

Keyword(s):

Lipid Peroxidation ◽

Carbon Tetrachloride ◽

Dietary Antioxidants ◽

Microsomal Lipid Peroxidation

Download Full-text

Hepatoprotective effect of hydroethanol extracts of Allium sativum and Carica papaya on CCl4- induced liver damage in rats

Nigerian Journal of Pure and Applied Sciences ◽

10.48198/njpas/20.a13 ◽

2020 ◽

pp. 3639-3649

Author(s):

I. O. Okoro

Keyword(s):

Lipid Peroxidation ◽

Allium Sativum ◽

Carica Papaya ◽

Serum Alkaline Phosphatase ◽

Synergistic Effects ◽

Substantial Reduction ◽

Negative Control ◽

Control Group ◽

Albino Rats ◽

Therapeutic Benefits

Plant-based products, believed to be less toxic have been employed as hepatoprotective agents in recent times. Polyherbal therapy is reported to have therapeutic benefits over single herb therapy. Allium sativum and Carica papaya are two known plants used traditionally for the treatment of liver related diseases. Thus, in this study, the hepatoprotective and antioxidant potential of hydroethanol extracts of Allium sativum cloves (Garlic) and Carica papaya (Pawpaw) leafs (singly and combined) against CCl4-induced toxicity in rats was evaluated. Thirty male albino rats used were classed into six (groups A- F) groups of five rats each and treated (orally) for 14 days. Group A- rats + distilled water (negative control); Group B- rats + CCl4 in olive oil (positive control); Group C- rats + 200 mg/ kg-day combined extract of A. sativum and C. papaya (1: 1 ratio); Group D- rats + 200 mg/ kg-day extract of A. sativum; Group E- rats + 200 mg/ kg-day extract of C. papaya; Group F- rats + 100 mg/kg bw of silymarin. CCl4 induce hepatotoxicity was characterized by an upsurge (P<0.05) in serum Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST) and Alanine Transaminase (ALT) activities, and total bilirubin. Also, CCl4 administration led to a substantial reduction (P<0.05) of reduced glutathione (GSH), Glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) activities and an increase (P<0.05) in lipid peroxidation (MDA) in the liver tissue. However, treatment with hydroethanol extract of A. sativum and C. papaya prevented the CCl4 induced lipid peroxidation and returned the altered marker enzymes of serum and antioxidant enzymes level to near normal. The extracts of both plants demonstrated significant antioxidant and hepatoprotective activity as reflected by the results. Comparatively, the combined extract gave the most promising antioxidant and hepatoprotective potential, which may be due to positive synergistic effects of both plants over either plant used singly, thereby vindicating the use of polyherbal remedy over a single herb.

Download Full-text