scholarly journals Effect of Grape Seed Oil on Chronic Carbon Tetrachloride-Induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats

2019 ◽  
pp. 1-10
Author(s):  
Ayhan Atasever ◽  
Ahmet Alpay ◽  
Gorkem Ekebas ◽  
Duygu Yaman Gram
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Caspase 3 ◽  
Seed Oil ◽  
Antioxidant Properties ◽  
Elevated Serum ◽  
Grape Seed ◽  
Control Group ◽  
Albino Rats ◽  
Grape Seed Oil

Aims: The present study was designed to evaluate the hepatoprotective activity of grape seed oil (GSO) on liver lesions induced by carbon tetrachloride (CCl4) in rats. The effects on hepatic injury were investigated by measuring serum levels of ALT, triglyceride, total protein, total cholesterol and liver levels of MDA. Furthermore, caspase -3, -8 and -9 activities in cellular apoptosis were determined. Place and Duration of Study: Faculty of Veterinary Medicine, Department of Pathology, Erciyes University, Kayseri, between November 2017 and September 2018. Methodology: In this study 40 male Wistar albino rats were divided into four groups including 10 animals in each. Control group administered with 0.9% NaCl. The second group was administered with 4 mL/kg GSO for twelve weeks. Third group were given CCl4 (0.2 mL/kg) twice for 8-weeks. Fourth group was administered with 4 mL/kg GSO, for 12 weeks and also given CCl4 (0.2 mL/kg) twice for 8 weeks, starting from the 5th week. Results: Histopathological examination of CCl4 group showed intense macro and micro vesicular steatosis in hepatocytes, necrosis, and lymphocytes rich mononuclear cell infiltration in portal area and mild portal fibrosis in the parenchyma. The grape seed oil applications have partially normalized the altered histological changes and the activity of caspase -3, -8 and -9. Administration of GSO led to a decline in the activities of ALT and MDA levels while this treatment elevated serum triglycerides levels which are not significantly important. Conclusion: The results indicate that the antioxidant properties of GSO have not ameliorative effect in either the histopathological lesions or biochemical parameters against CCl4-induced hepatotoxicity in rats. Also, it has been concluded that duration‐dependent further research results are needed to determine the effects of grape seed oil in high doses which can give the best results without side effects.

Efficiency of Grape (Vitis vinifera) Seed Oil on Nonspecific Immune Response and Histopathological Effects in Common Carp Cyprinus carpio Challenged with Pseudomonas aeruginosa

2021 ◽  
Vol 5 (1) ◽  
pp. 130-140
Author(s):  
Israa Al-Atbee
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cyprinus Carpio ◽  
Phagocytic Activity ◽  
Seed Oil ◽  
Grape Seed ◽  
Lysozyme Activity ◽  
Control Group ◽  
Treatment Groups ◽  
Grape Seed Oil ◽  
Histopathological Effects

The present study was carried out to assess the effect of grape seed oil supplemented diet on non- specific immunity of Cyprinus carpio against the pathogenic bacteria Pseudomonas aeruginosa. Fish weights 41.69±2.25 g and total length 13.65±1.24 cm were randomly distributed into four treatments (two replications for each treatment). Fish groups were fed four dissimilar diets up to 40 days. The first group (T1) was fed with 0.2% grape seed oil, the second group (T2) was fed supplemented with 0.5% grape seed oil, the third group (T3) was supplemented with 1% grape seed oil and the forth group (T4) was served as the control group which fed basal diet without supplementing with grape seed oil. Forty-six fishes in all treatment groups were challenged intramuscularly with P. aeruginosa on day 41. The fishes in experimental groups were challenged intramuscularly with 0.2 ml P. aeruginosa at a concentration of 108 CFU/ml and after challenge (14 days), the different parameters were determined including nitroblue tetrazolium % (NBT), myeloperoxidases % (MPO), phagocytic activity (%) and serum lysozyme activity (U/min). The results indicated that T2 group had significant increased (p<0.05) in NBT activity, MPO activity, phagocytic activity and lysozyme activity compared with control group and to other treatment groups, followed by T1 and T3, respectively. In addition, T2 showed highest resistance to challenge P. aeruginosa compared with other groups. T3 and T4 groups showed a decreased performance in all non-specific immune parameters. There were histopathological effects in liver, showing a focal region of lymphocyte aggregation (T3), and hemorrhage into the hepatic vein with infiltration of inflammatory cells (T4). Therefore, these results indicated that 0.5% grape seed oil (T2) as additive fed could be used as prophylactic in common carp culture to enhance the protection against any possible infection by P. aeruginosa.

scholarly journals Effect of Flax Seed Oil on Acute Carbon Tetrachloride-induced Hepatic Injury and Determination of Hepatic Apoptosis in Rats

2019 ◽  
pp. 1-10
Author(s):  
Gorkem Ekebas ◽  
Ayhan Atasever ◽  
Duygu Yaman Gram
Keyword(s):  
Carbon Tetrachloride ◽  
Total Cholesterol ◽  
Total Protein ◽  
Caspase 3 ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Treated Group ◽  
Flaxseed Oil ◽  
Albino Rats ◽  
Intraperitoneal Dose

Aims: The present study was designed to evaluate the hepatoprotective activity of flaxseed oil (FSO) on liver lesions induced by carbon tetrachloride (CCl4) in rats by measurement of caspase 3, 8 and 9 activities in cellular apoptosis, ALT activities, triglyceride, total protein, total cholesterol and liver MDA levels. Place and Duration of Study: Faculty of Veterinary Medicine, Department of Pathology, Erciyes University, Kayseri, between June 2017 and July 2018. Methodology: In this study 32 male Wistar albino rats were divided into four groups of 8 animals in each. The first group was identified as the control and received an intraperitoneal 0.9% NaCl and the second group was given per os at dose of 4 ml/kg FSO for 4 weeks. The third group received an intraperitoneal dose of 1.0 ml/kg CCl4 twice in the first week. The fourth group received an intraperitoneal dose of 1.0 ml/kg CCl4 twice in the first week and simultaneously 4 ml/kg FSO by gavage for 4 weeks. Results: Histopathological examination of CCl4 group showed intense macro and micro vesicular steatosis in hepatocytes, necrosis, lymphocytes rich mononuclear cell infiltration in portal area and parenchyma. The flaxseed oil application did not ameliorate the histological changes induced by CCl4, however reduced the activity of caspase 3, 8 and 9 by a limited number. CCl4 administration produced significantly elevated levels of serum ALT activity, total cholesterol, triglyceride and liver MDA levels, and these increases were not normalized with FSO treatment. In addition, decreased serum total protein levels in CCl4 treated group were ameliorated by FSO application. Conclusion: The results indicate that the antioxidant properties of FSO do not have an ameliorative effect in either the histopathological lesions or biochemical parameters against CCl4-induced hepatotoxicity in rats. In addition, it was concluded that duration‐dependent further research results are needed to determine the effects of flaxseed oil in high doses that can give the best results without side effects.

scholarly journals Comparing the Effect of Foot Massage with Grape Seed Oil and Sweet Almond Oil on Physiological Leg Edema in Primigravidae: A Randomized Clinical Trial

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Maryam Navaee ◽  
Marzieh Rakhshkhorshid
Keyword(s):  
Clinical Trial ◽  
Pregnant Women ◽  
Randomized Clinical Trial ◽  
Seed Oil ◽  
Grape Seed ◽  
Control Group ◽  
Almond Oil ◽  
Grape Seed Oil ◽  
Leg Edema ◽  
Sweet Almond

Background. Leg edema is a prevalent problem in pregnancy causing activity restrictions for pregnant women. This study was performed to compare the effect of foot massage using grape seed oil and sweet almond oil on physiological leg edema. Methods. A randomized clinical trial was conducted on 90 primigravidae referred to public health centres of Zahedan, Iran. The participants’ gestational age was 30–40 weeks. The study was conducted from August 2016 to November 2017. The participants were randomly assigned to three groups (massage with grape seed oil, massage with sweet almond oil, and without intervention). After determining the extent of leg edema, foot massages were done for 20 minutes within 5 days in the two intervention groups. Then, foot circumferences were measured on day 5 after the intervention. Foot circumferences for the control group were measured on days 1 and 5. A nonelastic tape measure was used to measure the circumferences. To analyse the data, SPSS 21 software and statistical tests including one-way ANOVA, Tukey’s test, and paired t-test were used. Results. The results from this study showed a significant difference in the mean score change of foot circumferences between groups (P=0.001). According to the results of Tukey’s test, mean score changes of foot circumferences of both intervention groups were significantly different those of the control group. However, this difference was not significant between the two intervention groups (P=0.865). Conclusion. The findings of this study confirmed the effectiveness of foot massage using grape seed and sweet almond oils to reduce pregnancy physiological edema. Therefore, foot massage with appropriate oils can be used as a useful technique by trained midwives in prenatal care centres or at pregnant women houses. This trial is registered with IRCT2015072723370N1.

Carbon Tetrachloride-Induced Hepatotoxicity: Protective Effect of 'Rocket' Eruca sativa L. in Rats

2010 ◽  
Vol 38 (01) ◽  
pp. 75-88 ◽  
Author(s):  
Saleh Alqasoumi
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Liver Tissue ◽  
Hepatic Injury ◽  
Liver Toxicity ◽  
Elevated Serum ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Eruca Sativa ◽  
Protein Sulfhydryl

The hepatoprotective and antioxidant effect of an ethanolic extract of 'Rocket' Eruca sativa L. (EER), on liver injury induced by carbon tetrachloride ( CCl4 ) was investigated. Wistar albino rats were administered 250 and 500 mg/kg body weight extract orally for 10 consecutive days. Marker enzymes GOT, GPT, ALP, GGT and bilirubin were estimated in serum. Whereas, non-protein sulfhydryl (NP-SH), total protein (TP) and malondialdehyde (MDA) were estimated in liver tissue as markers for oxidative stress. Histopathological assessment was also done on liver tissue. CCl4 induced liver poisoning in all treated animals was evident by elevated serum GOT, GPT, ALP, GGT and bilirubin levels. Induction of oxidative stress in the liver tissue by CCl4 was evidenced by a fall in the levels of NP-SH and TP; and an increased level of MDA concentration. EER administration for 10 days prevented the CCl4 induced hepatic injury and oxidative stress. Furthermore, the extract also reduced the pentobarbital-induced prolongation of sleeping time in mice. The ability of rocket extract to protect the liver toxicity in rats was further confirmed by histological findings in the liver tissue. In conclusion, it was observed that Eruca sativa L. extract protects the liver against CCl4 induced hepatic injury through its potent antioxidant activity in rats.

The Hepatoprotective Role of Warburgia salutaris and Iso-Mukaadial Acetate on Carbon tetrachloride Intoxicated Rats Model

2021 ◽  
Vol 17 ◽  
Author(s):  
Gideon Ayeni ◽  
Mthokozisi Blessing Cedric Simelane ◽  
Shahidul Islam ◽  
Ofentse Jacob Pooe
Keyword(s):  
Carbon Tetrachloride ◽  
Hepatic Injury ◽  
Antioxidant Properties ◽  
Hepatic Necrosis ◽  
Protective Role ◽  
Dependent Manner ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Isolated Compound

Background: Medicinal plants together with their isolated bioactive compounds are known for their antioxidant properties which constitute therapeutic agents that are routinely employed in the treatment of liver diseases. Aims of the Study: The current study sought to explore the protective role of Warburgia salutaris and its isolated compound, iso-mukaadial acetate against carbon tetrachloride (CCl4)-induced hepatic injury. Methods: Thirty-five male Sprague Dawley rats were divided into seven groups of five animals each and injected with CCl4 to induce hepatic injury. Results: Treatment with the crude extract of W. salutaris and of iso-mukaadial acetate significantly reduced the levels of alkaline phosphatase, alanine and aspartate aminotransaminases, total bilirubin and malondialdehyde in a dose dependent manner, when compared to untreated groups. Liver histology revealed a reduction in hepatic necrosis and inflammation. Conclusion: The current investigation has demonstrated that W. salutaris extract and iso-mukaadial acetate could mitigate the acute liver injury inflicted by a hepatotoxic inducer in rats.

Hypolipidemic effect of prepared Polyherbal formulations in Wistar albino rats

2021 ◽  
pp. 4314-4320
Author(s):  
Ranjan Kumar Giri ◽  
Sunil Kumar Kanungo ◽  
Saroj Kumar Patro ◽  
Minaketan Sahoo ◽  
Dibya Sundar Panda
Keyword(s):  
Elevated Serum ◽  
Density Lipoprotein ◽  
Lipid Lowering ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Study ◽  
Oral Glucose ◽  
Hypolipidemic Effect ◽  
Standard Drug ◽  
Wistar Albino Rats

Lipid lowering effect of polyherbal formulations using eight different plants was evaluated in triton and diet induced hyperlipidemic models of wistar albino rats. Formulations such as Tablet, Syrup and Suspension inhibited the elevation in serum cholesterol and triglyceride levels on Triton WR 1339 administration rats. The formulations at the same dose level significantly attenuated the elevated serum total cholesterol and triglycerides with an increase in high-density lipoprotein cholesterol in high-fat diet-induced hyperlipidemic rats. The standard drug Niacin showed slightly better effects. The treatment with herbal formulations produced 30-35 percentage improvement in oral glucose tolerance. Similarly all the formulations also reduced the elevated C-reactive protein which is a marker of Hyperlipidemia. In histopathological study it was found that treatment of polyherbal formulation significantly reduced the plaque size in aorta compared with HFD treated control group. The outcome of the study reveals the lipid lowering activity of polyherbal formulations in dyslipidaemic conditions by interfering with the biosynthesis of cholesterol and utilization of lipids.

scholarly journals AMELIORATIVE EFFECTS OF GRAPE SEED OIL ON CHROMIUM-INDUCED NEPHROTOXICITY AND OXIDATIVESTRESS IN RATS

2020 ◽  
Vol 57 (3) ◽  
Author(s):  
Sahar Hassan Orabi ◽  
Sherif Mohamed Shawky
Keyword(s):  
Dna Damage ◽  
Hexavalent Chromium ◽  
Seed Oil ◽  
Potassium Dichromate ◽  
Serum Levels ◽  
Grape Seed ◽  
Renal Tissue ◽  
Male Rats ◽  
Group I ◽  
Grape Seed Oil

The current study focused on investigating the renoprotective effects of grape seed oil (GSO) against hexavalent chromium (Cr (VI))-induced nephrotoxicity. A total of 40 male rats were randomly divided into four groups: group I served as the control group, group II received 1000 mg/L potassium dichromate (353.5 mg/L Cr(VI)) in drinking water for 12 weeks, group III received 3.7 g/kg body weight/day GSO orally for 12 weeks, and group IV received GSO together with potassium dichromate for 12 weeks. Cr(VI) significantly increased serum levels of urea, creatinine, potassium and glucose. In addition, Cr(VI) increased MDA levels and induced renal tissue damage and DNA damage. On the other hand, Cr(VI) decreased serum levels of sodium and antioxidant defence system [reduced glutathione (GSH) and catalase (CAT)]. However, treatment with GSO prevented elevation levels of serum urea, creatinine, potassium and glucose. In addition, GSO enhanced sodium level, renal tissue antioxidant defense system due to its curative effect ameliorated particularly oxidative stress, renal tissue and DNA damage. In conclusion, these results demonstrate that GSO is a promising nephroprotective agent against Cr(VI)-induced nephrotoxicity.Key words: grape seed oil; hexavalent chromium; nephrotoxicity; DNA damage BLAŽILNI UČINKI OLJA GROZDNIH PEŠK PRI TOKSIČNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, POVZROČEN S KROMOM Povzetek: Študija je bila osredotočena na proučevanje zaščitnih učinkov olja grozdnih pešk (GSO) pri toksični obremenitvi ledvic, povzročeni s heksavalentnim kromom (Cr (VI)). Štirideset samcev podgan je bilo naključno razdeljenih v štiri skupine: skupina I - kontrolna skupina, skupina II, ki je v pitni vodi 12 tednov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI)), skupina III, ki je peroralno 12 tednov prejemala 3,7 g/kg telesne mase/dan GSO ter skupina IV, ki je 12 tednov prejemala GSO skupaj s kalijevim dikromatom. Cr(VI) je znatno zvišal serumske ravni sečnine, kreatinina, kalija in glukoze v serumu. Poleg tega je Cr(VI) zvišal raven MDA in povzročil poškodbe ledvičnega tkiva in poškodbe DNK. Po drugi strani je Cr(VI) znižal serumsko raven natrija in antioksidativnega obrambnega sistema, zmanjšal raven glutationske peroksidaze in katalaze. Dodajanje GSO poskusnim živalim je preprečilo zvišanje ravni sečnine v serumu, kreatinina, kalija, natrija in glukoze. Poleg tega je GSO izboljšal obrambni sistem antioksidantov ledvičnega tkiva. Zaradi svojega zdravilnega učinka je izboljšal zlasti oksidativni stres, poškodbe ledvičnega tkiva in DNK. Rezultati kažejo, da je GSO obetavno zaščitno sredstvo za ledvica pri toksični obremenitvi, povzročeni s Cr(VI).Ključne besede: olje grozdnih pešk; heksavalentni krom; nefrotoksičnost; poškodba DNK

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