Cleavage of the angiotensin II type 1 receptor and nuclear accumulation of the cytoplasmic carboxy-terminal fragment
Our published studies show that the distribution of the ANG II type 1 (AT1) receptor (AT1R), expressed as a enhanced yellow fluorescent fusion (YFP) protein (AT1R/EYFP), is altered upon cellular treatment with ANG II or coexpression with intracellular ANG II. AT1R accumulates in nuclei of cells only in the presence of ANG II. Several transmembrane receptors are known to accumulate in nuclei, some as holoreceptors and others as cleaved receptor products. The present study was designed to determine whether the AT1R is cleaved before nuclear transport. A plasmid encoding a rat AT1R labeled at the amino terminus with enhanced cyan fluorescent protein (CFP) and at the carboxy terminus with EYFP was employed. Image analyses of this protein in COS-7 cells, CCF-STTG1 glial cells, and A10 vascular smooth muscle cells show the two fluorescent moieties to be largely spatially colocalized in untreated cells. ANG II treatment, however, leads to a separation of the fluorescent moieties with yellow fluorescence accumulating in more than 30% of cellular nuclei. Immunoblot analyses of extracts and conditioned media from transfected cells indicate that the CFP domain fused to the extracellular amino-terminal AT1R domain is cleaved from the membrane and that the YFP domain, together with the intracellular cytoplasmic carboxy terminus of the AT1R, is also cleaved from the membrane-bound receptor. The carboxy terminus of the AT1R is essential for cleavage; cleavage does not occur in protein deleted with respect to this region. Overexpressed native AT1R (nonfusion) is also cleaved; the intracellular 6-kDa cytoplasmic domain product accumulates to a significantly higher level with ANG II treatment.