Exercise restores decreased physical activity levels and increases markers of autophagy and oxidative capacity in myostatin/activin-blocked mdx mice

2013 ◽  
Vol 305 (2) ◽  
pp. E171-E182 ◽  
Author(s):  
Juha J. Hulmi ◽  
Bernardo M. Oliveira ◽  
Mika Silvennoinen ◽  
Willem M. H. Hoogaars ◽  
Arja Pasternack ◽  
...  
Keyword(s):  
Physical Activity ◽  
Body Mass ◽  
Wheel Running ◽  
Oxidative Capacity ◽  
Voluntary Exercise ◽  
Muscle Size ◽  
Mdx Mice ◽  
Activity Levels ◽  
Muscle Oxidative Capacity ◽  
Running Activity

The importance of adequate levels of muscle size and function and physical activity is widely recognized. Myostatin/activin blocking increases skeletal muscle mass but may decrease muscle oxidative capacity and can thus be hypothesized to affect voluntary physical activity. Soluble activin receptor IIB (sActRIIB-Fc) was produced to block myostatin/activins. Modestly dystrophic mdx mice were injected with sActRIIB-Fc or PBS with or without voluntary wheel running exercise for 7 wk. Healthy mice served as controls. Running for 7 wk attenuated the sActRIIB-Fc-induced increase in body mass by decreasing fat mass. Running also enhanced/restored the markers of muscle oxidative capacity and autophagy in mdx mice to or above the levels of healthy mice. Voluntary running activity was decreased by sActRIIB-Fc during the first 3–4 wk correlating with increased body mass. Home cage physical activity of mice, quantified from the force plate signal, was decreased by sActRIIB-Fc the whole 7-wk treatment in sedentary mice. To understand what happens during the first weeks after sActRIIB-Fc administration, when mice are less active, healthy mice were injected with sActRIIB-Fc or PBS for 2 wk. During the sActRIIB-Fc-induced rapid 2-wk muscle growth period, oxidative capacity and autophagy were reduced, which may possibly explain the decreased running activity. These results show that increased muscle size and decreased markers of oxidative capacity and autophagy during the first weeks of myostatin/activin blocking are associated with decreased voluntary activity levels. Voluntary exercise in dystrophic mice enhances the markers of oxidative capacity and autophagy to or above the levels of healthy mice.

Provision of a voluntary exercise environment enhances running activity and prevents obesity in Snark-deficient mice

2009 ◽  
Vol 296 (5) ◽  
pp. E1013-E1021 ◽  
Author(s):  
Noriko Ichinoseki-Sekine ◽  
Hisashi Naito ◽  
Katsuya Tsuchihara ◽  
Ikumi Kobayashi ◽  
Yuji Ogura ◽  
...  
Keyword(s):  
Wheel Running ◽  
Acute Effect ◽  
Voluntary Exercise ◽  
Activity Levels ◽  
Term Experiment ◽  
Running Activity ◽  
Voluntary Running ◽  
Short Term Experiment ◽  
Sedentary Conditions ◽  
Deficient Mice

The present study was performed to investigate the involvement of SNARK in physical activity levels in mice. To examine the acute effect of SNARK deficiency on voluntary running, Snark-deficient mice ( Snark+/−: n = 16) and their wild-type counterparts ( Snark+/+: n = 16) were assigned to sedentary or exercise (1 wk voluntary wheel running) groups. In addition, to clarify the differences in voluntary running activity and its effect between genotypes, mice ( Snark+/+: n = 16; Snark+/−: n = 16) were also kept in individual cages with/without a running wheel for 5 mo. Unexpectedly, in both voluntary running experiments, running distances were increased in Snark+/− mice compared with Snark+/+ mice. Under sedentary conditions, body and white adipose tissue weights were increased significantly in Snark+/− mice. However, no significant differences were observed between the two genotypes under exercise conditions, and the values were significantly less than those under sedentary conditions in the long-term experiment. In the short-term experiment, serum interleukin-6 level in exercised Snark+/+ mice was the same as that in sedentary Snark+/+ mice, whereas that in sedentary Snark+/− mice was significantly lower than in the other groups. In contrast, serum leptin level was reduced significantly in exercised Snark+/− mice compared with sedentary Snark+/− mice. The results of this study demonstrated that exposure to an environment that allows voluntary exercise promotes increased running activity and prevents obesity in Snark-deficient mice.

Skeletal muscle oxidative capacity in young and older women and men

2000 ◽  
Vol 89 (3) ◽  
pp. 1072-1078 ◽  
Author(s):  
Jane A. Kent-Braun ◽  
Alexander V. Ng
Keyword(s):  
Physical Activity ◽  
Skeletal Muscle ◽  
Recovery Time ◽  
Tibialis Anterior Muscle ◽  
Oxidative Capacity ◽  
Resonance Spectroscopy ◽  
Activity Levels ◽  
Muscle Oxidative Capacity ◽  
Age Related ◽  
Postexercise Recovery

It has been suggested that a decline in skeletal muscle oxidative capacity is a general consequence of aging in humans. However, previous studies have not always controlled for the effects of varying levels of physical activity on muscle oxidative capacity. To test the hypothesis that, when matched for comparable habitual physical activity levels, there would be no age-related decline in the oxidative capacity of a locomotor muscle, the postexercise recovery time of phosphocreatine was compared in the tibialis anterior muscle of young [ n = 19; 33.8 ± 4.8 (SD) yr] and older [ n = 18; 75.5 ± 4.5 yr] healthy women and men of similar, relatively low, activity levels. The intramuscular metabolic measurements were accomplished by using phosphorus magnetic resonance spectroscopy. The results indicate that there was no age effect on the postexercise recovery time of phosphocreatine recovery, thus supporting the stated hypothesis. These data suggest that there is no requisite decline in skeletal muscle oxidative capacity with aging in humans, at least through the seventh decade.

Voluntary exercise decreases progression of muscular dystrophy in diaphragm of mdx mice

1994 ◽  
Vol 77 (4) ◽  
pp. 1736-1741 ◽  
Author(s):  
E. E. Dupont-Versteegden ◽  
R. J. McCarter ◽  
M. S. Katz
Keyword(s):  
Muscular Dystrophy ◽  
Muscle Function ◽  
Wheel Running ◽  
Control Level ◽  
Contractile Properties ◽  
Voluntary Exercise ◽  
Mdx Mice ◽  
Running Activity ◽  
Tension Generation

Effects of voluntary wheel running on contractile properties of diaphragm (DIA) and soleus (SOL) of dystrophic (mdx) and control (C57BL/10SNJ) mice were evaluated. In particular, we tested the hypothesis that daily voluntary running is not deleterious to muscle function in mdx mice. Both groups of mice ran extensively (control mice approximately 7 km/day, mdx mice approximately 5 km/day). Exercise increased maximal specific tetanus tension of mdx DIA from 1.02 +/- 0.04 to 1.33 +/- 0.06 kg/cm2 but did not restore it to the control level (2.55 +/- 0.17 kg/cm2). Maximal tetanus tension of sedentary mdx SOL (2.41 +/- 0.17 kg/cm2) was reduced compared with control (3.10 +/- 0.15 kg/cm2) and was not altered by running activity. Optimal length was significantly lower in DIA of mdx mice, and exercise did not change this. Fatigability and contractile properties of muscles measured in vitro were not altered by running activity with the exception of increased contraction time in mdx DIA. In conclusion, extensive wheel running is not deleterious to muscle function in mdx mice contrary to predictions of the “work overload” theory of muscular dystrophy. Rather, this exercise is beneficial for active tension generation of mdx DIA, the muscle most closely resembling muscles of patients with Duchenne muscular dystrophy.

scholarly journals Player Tracking Data Analytics as a Tool for Physical Performance Management in Football: A Case Study from Chelsea Football Club Academy

Sports ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 130 ◽  
Author(s):  
Varuna De Silva ◽  
Mike Caine ◽  
James Skinner ◽  
Safak Dogan ◽  
Ahmet Kondoz ◽  
...  
Keyword(s):  
Physical Activity ◽  
High Speed ◽  
Activity Level ◽  
Activity Levels ◽  
Gradual Change ◽  
Distribution Analysis ◽  
Tracking Data ◽  
Running Activity ◽  
Player Tracking ◽  
Movement Tracking

Background: Global positioning system (GPS) based player movement tracking data are widely used by professional football (soccer) clubs and academies to provide insight into activity demands during training and competitive matches. However, the use of movement tracking data to inform the design of training programmes is still an open research question. Objectives: The objective of this study is to analyse player tracking data to understand activity level differences between training and match sessions, with respect to different playing positions. Methods: This study analyses the per-session summary of historical movement data collected through GPS tracking to profile high-speed running activity as well as distance covered during training sessions as a whole and competitive matches. We utilise 20,913 data points collected from 53 football players aged between 18 and 23 at an elite football academy across four full seasons (2014–2018). Through ANOVA analysis and probability distribution analysis, we compare the activity demands, measured by the number of high-speed runs, the amount of high-speed distance, and distance covered by players in key playing positions, such as Central Midfielders, Full Backs, and Centre Forwards. Results and Implications: While there are significant positional differences in physical activity demands during competitive matches, the physical activity levels during training sessions do not show positional variations. In matches, the Centre Forwards face the highest demand for High Speed Runs (HSRs), compared to Central Midfielders and Full Backs. However, on average the Central Midfielders tend to cover more distance than Centre Forwards and Full Backs. An increase in high-speed work demand in matches and training over the past four seasons, also shown by a gradual change in the extreme values of high-speed running activity, was also found. This large-scale, longitudinal study makes an important contribution to the literature, providing novel insights from an elite performance environment about the relationship between player activity levels during training and match play, and how these vary by playing position.

Beneficial effects of voluntary wheel running on the properties of dystrophic mouse muscle

1996 ◽  
Vol 80 (2) ◽  
pp. 670-679 ◽  
Author(s):  
A. Hayes ◽  
D. A. Williams
Keyword(s):  
Skeletal Muscles ◽  
Wheel Running ◽  
Voluntary Exercise ◽  
Mdx Mice ◽  
Free Access ◽  
Type I ◽  
Fiber Types ◽  
Force Output ◽  
Mouse Muscle ◽  
Beneficial Effects

Effects of voluntary exercise on the isometric contractile, fatigue, and histochemical properties of hindlimb dystrophic (mdx and 129ReJ dy/dy) skeletal muscles were investigated. Mice were allowed free access to a voluntary running wheel at 4 wk of age for a duration of 16 (mdx) or 5 (dy/dy) wk. Running performance of mdx mice (approximately 4 km/day at 1.6 km/h) was inferior to normal mice (approximately 6.5 km/day at 2.1 km/h). However, exercise improved the force output (approximately 15%) and the fatigue resistance of both C57BL/10 and mdx soleus muscles. These changes coincided with increased proportions of smaller type I fibers and decreased proportions of larger type IIa fibers in the mdx soleus. The extensor digitorum longus of mdx, but not of normal, mice also exhibited improved resistance to fatigue and conversion towards oxidative fiber types. The dy/dy animals were capable of exercising, yet ran significantly less than normal animals (approximately 0.5 km/day). Despite this, running increased the force output of the plantaris muscle (approximately 50%). Taken together, the results showed that exercise can have beneficial effects on dystrophic skeletal muscles.

scholarly journals Objectively measured physical activity levels in physical education classes and body mass index (Niveles de actividad física medida objetivamente en las clases de educación física y el índice de masa grasa)

Retos ◽  
2016 ◽  
pp. 271-274
Author(s):  
Manuela Costa ◽  
Tânia Oliveira ◽  
Jorge Mota ◽  
Maria Paula Santos ◽  
José Carlos Ribeiro
Keyword(s):  
Physical Activity ◽  
Body Mass Index ◽  
Public Schools ◽  
Physical Education ◽  
Body Mass ◽  
Vigorous Physical Activity ◽  
The Body ◽  
Activity Levels ◽  
Relative Contribution ◽  
Objectively Measured

Objective: The objective of this study was twofold. First, analyze physical activity (PA) levels during physical education (PE) with different durations (45 and 90 minutes) according to student’s obesity status. Secondly, we examine the relative contribution of 45 and 90 minutes PE (45PE and 90PE) for the compliance of the daily PA recommendations according to the body mass index (BMI). Methods: Four public schools were analyzed. The sample comprised 472 youngsters (266 girls) aged between 10 and 18 years old. PA was assessed using an Actigraph accelerometer. The participants were categorized as non-overweight (NOW) and overweight/obese (OW) according to the sex-adjusted BMI. Results: The proportion of Moderate and Vigorous Physical Activity (MVPA) was lower than the 50% recommended by guidelines regardless the PE duration. Our data showed that only 26% of NOW and 13% of OW in the 45PE achieved the recommended levels while 17% of NOW and 11% of OW achieved the recommendation in 90PE. Overall, the 90PE had a higher absolute contribution for daily MVPA recommendations compliance than 45PE. Conclusion: During PE classes youngsters spent a reduced amount of time in MVPA, independently of their weight status.Resumen. Objetivo: Este estudio tiene un doble objetivo. En primero lugar, analizar los niveles de la actividad física durante la educación física con diferentes duraciones (45 y 90 minutos) de acuerdo con el estado de la obesidad de los alumnos. En segundo lugar, se analiza la contribución relativa de 45 y 90 minutos de la educación física para el cumplimiento de las recomendaciones diarias de actividad física según el índice de masa grasa. Métodos: cuatro escuelas públicas fueran analizadas. La muestra fue de 472 jóvenes (266 chicas) con edades entre los 10 y 18 años. La actividad física fue medida utilizando un acelerómetro Actigraph. Los participantes fueran clasificados como sin sobrepeso y con sobrepeso/obesidad de acuerdo con el índice de masa grasa ajustado al género. Resultados: La proporción de la actividad física moderada y vigorosa fue inferior al 50% recomendado por las recomendaciones independiente de la duración de la clase de educación física. Nuestros datos muestran que solo unos 26% de los niños sin sobrepeso y unos 13% de niñoss con sobrepeso/obesidad llegaron al los niveles recomendados en las clases de 45 min, mientras el 17% de los jóvenes sin sobrepeso y el 11% con sobrepeso/obesidad han logrado las recomendaciones en las clases de 90 min. En general, las clases de 90 min tienen una mayor contribución para cumplimiento de las recomendaciones diarias de actividad física moderada a vigorosa do que las clases de 45 min. Conclusión: Durante las clases de educación física los jóvenes tuvieran una cantidad reducida de tiempo en actividad física moderada a vigorosa, independiente de su estado de peso.

scholarly journals Low Physical Activity Levels Are Linked to Early Hypertension Risk in College-Going Young Adults

Healthcare ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1258
Author(s):  
Kalyana Chakravarthy Bairapareddy ◽  
Mariam Mhd Salem Kamcheh ◽  
Ranim Jihad Itani ◽  
Mirna Mohamed ◽  
Heba Ayman Eid Abdellatif Zahran ◽  
...  
Keyword(s):  
Physical Activity ◽  
Body Mass Index ◽  
Young Adults ◽  
Waist Circumference ◽  
Body Mass ◽  
Activity Levels ◽  
Hypertension Risk ◽  
College Going ◽  
Physical Activity Levels ◽  
Weekly Physical Activity

Background: Sedentary behaviour and physical inactivity along with body mass are identified as critical determinants of vascular health along with body mass in young adults. However, the relationship between potential physical health and anthropometric variables with high blood Eid pressure remain unexplored in young adults from the United Arab Emirates region. Methodology: We administered a cross-sectional study in young adults assessing their self-reported physical activity levels, anthropometric variables (body mass index and waist circumference) and ambulatory blood pressure. The associations among potential physical health, anthropometric variables and high blood pressure were analysed through logistic regression after necessary transformation. Results: Of 354 participants (176 males, 178 females), we found 17.79% (n = 63) had higher mean arterial pressure. Males (n = 40; 22.73%) had higher risk of hypertension than females (n = 12.92%). Weekly physical activity levels (β = −0.001; p = 0.002), age (β = −0.168; p = 0.005) and gender (β = −0.709; p = 0.028) were found to be more strongly associated with hypertension risk than the body mass index (β = 0.093; p = 0.075), waist circumference (β = 0.013; p = 0.588) and the weekly sitting time (β = 0.000; p = 0.319) of the individuals. Conclusions: Lower physical activity was associated with hypertension risk compared to other modifiable risk factors such as waist circumference, body mass index and sedentary time in college-going young adults. Public health measures should continue to emphasise optimisation of weekly physical activity levels to mitigate vascular health risks at educational institution levels.

Genetic analysis of daily physical activity using a mouse chromosome substitution strain

2009 ◽  
Vol 39 (1) ◽  
pp. 47-55 ◽  
Author(s):  
He S. Yang ◽  
Martha H. Vitaterna ◽  
Aaron D. Laposky ◽  
Kazuhiro Shimomura ◽  
Fred W. Turek
Keyword(s):  
Physical Activity ◽  
Physical Activity Level ◽  
Wheel Running ◽  
Daily Physical Activity ◽  
Activity Level ◽  
Constant Darkness ◽  
Circadian Period ◽  
Chromosome 13 ◽  
Running Activity ◽  
Wheel Running Activity

There is considerable evidence for a genetic basis underlying individual differences in spontaneous physical activity in humans and animals. Previous publications indicate that the physical activity level and pattern vary among inbred strains of mice and identified a genomic region on chromosome 13 as quantitative trait loci (QTL) for physical activity. To confirm and further characterize the role of chromosome 13 in regulating daily physical activity level and pattern, we conducted a comprehensive phenotypic study in the chromosome 13 substitution strain (CSS-13) in which the individual chromosome 13 from the A/J strain was substituted into an otherwise complete C57BL/6J (B6) genome. The B6 and A/J parental strains exhibited pronounced differences in daily physical activity, sleep-wake structure, circadian period and body weight. Here we report that a single A/J chromosome 13 in the context of a B6 genetic background conferred a profound reduction in both total cage activity and wheel-running activity under a 14:10-h light-dark cycle, as well as in constant darkness, compared with B6 controls. Additionally, CSS-13 mice differed from B6 controls in the diurnal distribution of activity and the day-to-day variability in activity onset. We further performed a linkage analysis and mapped a significant QTL on chromosome 13 regulating the daily wheel running activity level in mice. Taken together, our findings indicate a QTL on chromosome 13 with dramatic and specific effects on daily voluntary physical activity, but not on circadian period, sleep, or other aspects of activity that are different between B6 and A/J strains.

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