Fibrinogen titer as an indicator of physiologic stability

1963 ◽  
Vol 204 (1) ◽  
pp. 42-44 ◽  
Author(s):  
J. M. McKenzie ◽  
D. R. Celander ◽  
M. M. Guest
Keyword(s):  
Environmental Stress ◽  
Tissue Damage ◽  
The Other ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Low Intensity ◽  
Electrical Stimuli

A semimicro method was developed and used to determine the levels of fibrinogen in two groups of dogs. One group was obtained from the dog pound and their plasma fibrinogen levels were determined immediately. The other group was kept in the departmental animal quarters under conditions essentially free of environmental stress for 45 days before determining the plasma fibrinogen concentration. The concentration of fibrinogen was found to be significantly lower in the group which was housed in the departmental quarters. However, when representative individuals from the latter group were subjected to intermittent electrical stimuli of low intensity for periods of 3–11 days the plasma fibrinogen increased significantly. It thus appears that the concentration of plasma fibrinogen is directly related to the degree of stress to which animals have been subjected and the stress initiating the response need not cause overt tissue damage.

Some Factors Influencing Metabolism and Distribution of Fibrinogen in Man and Rabbits

1977 ◽  
Vol 37 (02) ◽  
pp. 243-252
Author(s):  
Yi-Hsiang Chen ◽  
E. B Reeve
Keyword(s):  
Plasma Fibrinogen ◽  
Synthesis Rate ◽  
Fibrinogen Concentration ◽  
Fractional Catabolic Rate ◽  
System Parameters ◽  
Catabolic Rate ◽  
Simultaneous Decrease ◽  
Normal Males ◽  
Healthy Females ◽  
Made In

SummaryTo shed some light on the homeostatic regulation of plasma fibrinogen, metabolic studies were made in healthy females, and in normal, thyroidectomized, and thyroxine-treated rabbits. In females, compared with normal males, plasma fibrinogen concentration, plasma and interstitial fibrinogen decreased consequent to an increased fractional catabolic rate and a normal fibrinogen synthesis rate. The interstitial/plasma fibrinogen ratio remained unchanged. In normal rabbits, with increasing body weight fractional catabolic rate and catabolic rate decreased, while fibrinogen concentration and plasma fibrinogen remained constant owing to a simultaneous decrease in fibrinogen synthesis. In addition, fractional transcapillary transfer rate and transcapillary flux also decreased resulting in a shrinkage of interstitial fibrinogen. Thyroidectomy and thyroxine-injection markedly altered fibrinogen metabolism: thyroid hormone accelerated fibrinogen catabolism but also stimulated synthesis. The net result was an increase in plasma fibrinogen and fibrinogen concentration. The interstitial/plasma fibrinogen ratio decreased in thyroxine-treated, and increased in thyroidectomized animals. This study defines the variations of the fibrinogen system parameters in these physiologic and pathologic conditions, and illustrates some patterns of alterations in fibrinogen metabolism.

Factors Regulating Plasma Protein Synthesis IV. Influence of Fragments D and E on Plasma Fibrinogen Concentration

1974 ◽  
Vol 31 (03) ◽  
pp. 395-402 ◽  
Author(s):  
V Bocci ◽  
T Conti ◽  
M Muscettola ◽  
A Pacini ◽  
G. P Pessina
Keyword(s):  
Protein Synthesis ◽  
Plasma Protein ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration

SummaryRabbit fibrinogen digest products (FDP) have been separated by Pevikon block electrophoresis yielding fragments D, E and other unidentified FDP.The fragments were injected into rabbits. Surprisingly, as little as 4.3 mg of fragment D elicited a significant increase in plasma fibrinogen concentration 24 hr after injection. The stimulating activity of fragment D is at least 10-fold higher than that of fragment E.

Effects of smoking and abstention from smoking on fibrinogen synthesis in humans

2001 ◽  
Vol 100 (4) ◽  
pp. 459-465 ◽  
Author(s):  
Kirsty A. HUNTER ◽  
Peter J. GARLICK ◽  
Iain BROOM ◽  
Susan E. ANDERSON ◽  
Margaret A. McNURLAN
Keyword(s):  
Risk Factors ◽  
Significant Risk ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Primary Role ◽  
Absolute Rate ◽  
Albumin Synthesis ◽  
Plasma Albumin ◽  
Concomitant Reduction ◽  
Metabolic Mechanism

Cigarette smoking and hyperfibrinogenaemia are both significant risk factors for the development of cardiovascular disease. Two studies are described here which aimed to establish the metabolic mechanism responsible for the raised plasma fibrinogen concentration observed in smokers. Chronic smokers had a significantly elevated absolute rate of fibrinogen synthesis (ASR) compared with non-smokers (22.7±1.3 mg/kg per day versus 16.0±1.3 mg/kg per day; means±S.E.M., P < 0.01), with plasma levels of fibrinogen significantly correlated with fibrinogen synthesis (r = 0.65, P = 0.04). Unlike fibrinogen, plasma albumin concentrations were lower in smokers than in non-smokers (45±0.4 versus 47±0.7 g/l, P < 0.05), but there was no difference in rates of albumin synthesis between the two groups. Two weeks cessation from smoking by previously chronic smokers was associated with a rapid and marked fall in plasma fibrinogen concentration (from 3.06±0.11 g/l to 2.49±0.14 g/l, P < 0.001), and a significant reduction in ASR (a 33% reduction, from 24.1±1.7 to 16.1±1.0 mg/kg per day, P < 0.001). These studies suggest a primary role for increased synthesis in producing the hyperfibrinogenaemia associated with smoking. Moreover, abstention from smoking for a period of only 2 weeks induces a significant decrease in the rate of fibrinogen synthesis by the liver, with a concomitant reduction in the plasma fibrinogen concentration.

Fibrinogen measurement in cardiac surgery with cardiopulmonary bypass: Analysis of repeatability and agreement of Clauss method within and between six different laboratories

2014 ◽  
Vol 112 (07) ◽  
pp. 109-117 ◽  
Author(s):  
Ekaterina Baryshnikova ◽  
Armando Tripodi ◽  
Christoph J. Schlimp ◽  
Herbert Schöchl ◽  
Janne Cadamuro ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Cardiopulmonary Bypass ◽  
Plasma Fibrinogen ◽  
Detection Methods ◽  
Fibrinogen Concentration ◽  
Plasma Samples ◽  
Limits Of Agreement ◽  
Study Objective ◽  
Standard Operating ◽  
Mean Differences

SummaryPlasma fibrinogen concentration is important for coagulopathy assessment, and is most commonly measured using the Clauss method. Several factors, including device type and reagent, have been shown to affect results. The study objective was to evaluate performance and repeatability of the Clauss method and to assess differences between measurements performed during and after cardiopulmonary bypass (CPB), by testing plasma samples from patients undergoing cardiac surgery with CPB. Samples were collected from 30 patients before surgery, approximately 20 minutes before weaning from CPB, and 5 minutes after CPB and protamine. Fibrinogen concentration was determined using the Clauss method at six quality-controlled specialised laboratories, according to accredited standard operating procedures. Regarding within-centre agreement for Clauss measurement, mean differences between duplicate measurements were between 0.00 g/l and 0.15 g/l, with intervals for 95% limits of agreement for mean Bland-Altman differences up to 1.3 g/l. Regarding between-centre agreement, some mean differences between pairs of centres were above 0.5 g/l. Differences of up to ∼2 g/l were observed with individual samples. Increased variability was observed between centres, with inter-class correlation values below 0.5 suggesting only fair agreement. There were no significant differences in fibrinogen concentration before weaning from CPB and after CPB for most centres and methods. In conclusion, considerable differences exist between Clauss-based plasma fibrinogen measured using different detection methods. Nevertheless, the similarity between measurements shortly before weaning from CPB and after CPB within centres suggests that on-pump measurements could provide an early estimation of fibrinogen deficit after CPB and thus guidance for haemostatic therapy.

scholarly journals Regionální konvergence v příjmech v rámci odvětví české ekonomiky

2020 ◽  
Author(s):  
Pavel Zdražil
Keyword(s):  
Regional Disparities ◽  
The Other ◽  
Hours Worked ◽  
Crisis Period ◽  
Low Intensity ◽  
Sectoral Structure ◽  
Other Hand ◽  
Convergence Process ◽  
Sigma Convergence ◽  
Industrial Groups

The aim of this contribution is to assess the development of regional disparities in income within the sectors of Czech economy. Based on the average compensations of employees per hours worked, the sectoral structure is assessed at the level of 6 individual industries and 5 aggregated industrial groups. The research is carried out at the level of self-governing regions in 1995-2018. The sigma convergence approach is proceed to analyse the disparity processes. The analysis showed that individual sectors have very different positions in the economy in terms of both the share and dynamics of changes. Moreover, in the pre-crisis period (before 2008) the level and dynamics of variability were higher in most sectors. The results suggest that regional disparities in income are converging in most sectors; however, disparities are stable at the level of the whole economy. This development is a result of absence of convergence or divergence processes in sectors with large and growing shares on the structure of economy. On the other hand, the share of the converging sectors is stable or rather declining, while the convergence tendencies proceed with a very low intensity. Higher intensity of convergence process is achieved only by very minor sectors of economy.

scholarly journals Elevation of plasma fibrinogen in silent myocardial ischaemia.

1999 ◽  
Vol 46 (4) ◽  
pp. 985-989
Author(s):  
A Grzywacz ◽  
P Psuja ◽  
M Zozulińska ◽  
W Elikowski ◽  
K Zawilska
Keyword(s):  
Myocardial Ischaemia ◽  
Severe Disease ◽  
Stable Condition ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Activity Levels ◽  
Silent Myocardial Ischaemia ◽  
Group A ◽  
Group B ◽  
Pai 1

High plasma levels of fibrinogen and plasminogen activator inhibitor (PAI-1) are reported to be correlated with coronary heart disease. Therefore the level of fibrinogen concentration in plasma was examined and verified for the possible correlation with the previously explored PAI-1 antigen and PAI-1 activity in the pathogenesis of premature atherosclerosis (Grzywacz et al., 1998, Blood Coagul Fibrinol. 9, 245-249). Examination included only men, aged 33-46 years, who were in a stable condition for at least six months after the acute event. They were divided into two subgroups: group A (n = 14) with and group B (n = 15) without ischaemic changes in 24 h Holter electrocardiogram. The number of involved vessels visible on the coronarography picture was similar in both groups. In the patients of group A the mean level of fibrinogen (3.92 vs 3.23 g/l, P < 0.05) was higher than in the controls (n = 15). No statistically differences were found between group B and control healthy subjects in any of the parameters measured. There were no correlation between fibrinogen concentration and PAI-1 antigen and activity levels, which were elevated in both groups of patients according to our previous study. Our results indicate that elevated levels of plasma fibrinogen and PAI-1 appeared in the group of patients with more severe disease, as revealed by silent myocardial ischaemia.

scholarly journals Epithelial Conduction in Hydromedusae

1968 ◽  
Vol 52 (3) ◽  
pp. 600-621 ◽  
Author(s):  
G. O. Mackie ◽  
L. M. Passano
Keyword(s):  
Nervous System ◽  
Spontaneous Activity ◽  
Nervous Activity ◽  
The Other ◽  
Refractory Periods ◽  
Spread Of Excitation ◽  
Composite Events ◽  
Waves Propagation ◽  
Excess Mg ◽  
Electrical Stimuli

Sarsia, Euphysa, and other hydromedusae have been studied by electrophysiological techniques and are found to have nonnervous conducting epithelia resembling those described earlier for siphonophores. Simple, nonmuscular epithelia fire singly or repetitively following brief electrical stimuli. The pulses recorded with suction electrodes are biphasic, initially positive, and show amplitudes of 0.75–2.0 mv, durations of 5–15 msec, and velocities of 15–35 cm/sec with short refractory periods. In the swimming muscle (myoepithelium) 2.0–4.0 mv composite events lasting 150–300 msec are associated with contraction waves. Propagation in nonnervous epithelia is typically all-or-none, nondecremental, and unpolarized. The subumbrellar endoderm lamella conducts independently of the adjacent ectoderm. The lower regions of the tentacles do not show propagated epithelial events. The spread of excitation in conducting epithelia and associated effector responses are described. Examples are given of interaction between events seemingly conducted in the nervous system and those in nonnervous epithelia. Either system may excite the other. Spontaneous activity, however, appears to originate in the nervous system. Conduction in nonnervous tissues is unaffected by excess Mg++ in concentrations suppressing presumed nervous activity, although this may not be a wholly adequate criterion for distinguishing components of the two systems. Evidence from old work by Romanes is considered in the light of these findings and the general significance of epithelial conduction is discussed.

Suppression of fibrin(ogen)-driven pathologies through controlled knockdown by lipid nanoparticle delivery of siRNA

Blood ◽  
2021 ◽  
Author(s):  
Lih Jiin Juang ◽  
Woosuk Steve Hur ◽  
Lakmali Munasinghage Silva ◽  
Amy W Strilchuk ◽  
Brenton Francisco ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Metastatic Potential ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Nanoparticle Delivery ◽  
Lipid Nanoparticle ◽  
Cytokine Levels ◽  
Endotoxemia Model ◽  
Peritonitis Model ◽  
Deficient Mice

Fibrinogen plays a pathologic role in multiple diseases. It contributes to thrombosis and modifies inflammatory and immune responses, supported by studies in mice expressing fibrinogen variants with altered function or with a germline fibrinogen deficiency. However, therapeutic strategies to safely and effectively tailor plasma fibrinogen concentration are lacking. Here, we developed a strategy to tune fibrinogen expression by administering lipid nanoparticle (LNP)-encapsulated siRNA targeting the fibrinogen α chain (siFga). Three distinct LNP-siFga reagents reduced both hepatic Fga mRNA and fibrinogen levels in platelets and plasma, with plasma levels decreased to 42%, 16% and 4% of normal within one-week of administration. Using the most potent siFga, circulating fibrinogen was controllably decreased to 32%, 14%, and 5% of baseline with a 0.5, 1, and 2 mg/kg dose, respectively. Whole blood from mice treated with siFga formed clots with significantly decreased clot strength ex vivo, but siFga treatment did not compromise hemostasis following saphenous vein puncture or tail transection. In an endotoxemia model, siFga suppressed the acute phase response and decreased plasma fibrinogen, D-dimer, and proinflammatory cytokine levels. In a sterile peritonitis model, siFga restored normal macrophage migration in plasminogen-deficient mice. Finally, treatment of mice with siFga decreased the metastatic potential of tumour cells in a manner comparable to that observed in fibrinogen-deficient mice. The results indicate that siFga causes robust and controllable depletion of fibrinogen and provide the proof-of-concept that this strategy can modulate the pleiotropic effects of fibrinogen in relevant disease models.

Terrorism and Democracy

2021 ◽  
Vol 20 (3) ◽  
pp. 344-379
Author(s):  
Andrey Korotayev ◽  
Ilya Vaskin ◽  
Daniil Romanov
Keyword(s):  
Positive Relationship ◽  
The Other ◽  
Terrorist Attacks ◽  
Democratic Regime ◽  
Political Regimes ◽  
Powerful Factor ◽  
Terrorist Activity ◽  
Low Intensity ◽  
High Level ◽  
The Relationship

Abstract This article proposes a new explanation of the positive correlation between democracy and terrorism detected in many previous studies. It is shown that this might be accounted for by the fact that factional democracies are subjected to more terrorist attacks than the other political regimes. A positive relationship between the democratic regime and the level of terrorist activity can be obtained due to the inclusion of factional democracies in the sample of democratic states. If factional democracies are excluded from the sample, the relationship between the level of terrorist activity and the democratic regime is negative. The analysis allows to maintain that factional democracy is a rather powerful factor of a high level of terrorist activity, while non-factional democracy turns out to be rather a statistically significant predictor of a relatively low intensity of terrorist attacks.

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