Detrimental effects of reloading recovery on force, shortening velocity, and power of soleus muscles from hindlimb-unloaded rats

2008 ◽  
Vol 295 (5) ◽  
pp. R1585-R1592 ◽  
Author(s):  
J. J. Widrick ◽  
G. F. Maddalozzo ◽  
H. Hu ◽  
J. C. Herron ◽  
U. T. Iwaniec ◽  
...  

To better understand how atrophied muscles recover from prolonged nonweight-bearing, we studied soleus muscles (in vitro at optimal length) from female rats subjected to normal weight bearing (WB), 15 days of hindlimb unloading (HU), or 15 days HU followed by 9 days of weight bearing reloading (HU-R). HU reduced peak tetanic force (Po), increased maximal shortening velocity (Vmax), and lowered peak power/muscle volume. Nine days of reloading failed to improve Po, while depressing Vmax and intrinsic power below WB levels. These functional changes appeared intracellular in origin as HU-induced reductions in soleus mass, fiber cross-sectional area, and physiological cross-sectional area were partially or completely restored by reloading. We calculated that HU-induced reductions in soleus fiber length were of sufficient magnitude to overextend sarcomeres onto the descending limb of their length-tension relationship upon the resumption of WB activity. In conclusion, the force, shortening velocity, and power deficits observed after 9 days of reloading are consistent with contraction-induced damage to the soleus. HU-induced reductions in fiber length indicate that sarcomere hyperextension upon the resumption of weight-bearing activity may be an important mechanism underlying this response.

1996 ◽  
Vol 80 (3) ◽  
pp. 981-987 ◽  
Author(s):  
J. J. Widrick ◽  
J. J. Bangart ◽  
M. Karhanek ◽  
R. H. Fitts

This study examined the effectiveness of intermittent weight bearing (IWB) as a countermeasure to non-weight-bearing (NWB)-induced alterations in soleus type I fiber force (in mN), tension (Po; force per fiber cross-sectional area in kN/m-2), and maximal unloaded shortening velocity (Vo, in fiber lengths/s). Adult rats were assigned to one of the following groups: normal weight bearing (WB), 14 days of hindlimb NWB (NWB group), and 14 days of hindlimb NWB with IWB treatments (IWB group). The IWB treatment consisted of four 10-min periods of standing WB each day. Single, chemically permeabilized soleus fiber segments were mounted between a force transducer and position motor and were studied at maximal Ca2+ activation, after which type I fiber myosin heavy-chain composition was confirmed by sodium dodecyl sufate-polyacrylamide gel electrophoresis. NWB resulted in a loss in relative soleus mass (-45%), with type I fibers displaying reductions in diameter (-28%) and peak isometric force (-55%) and an increase in Vo (+33%). In addition, NWB induced a 16% reduction in type I fiber Po, a 41% reduction in type I fiber peak elastic modulus [Eo, defined as (delta force/delta length) x (fiber length/fiber cross-sectional area] and a significant increase in the Po/Eo ratio. In contrast to NWB, IWB reduced the loss of relative soleus mass (by 22%) and attenuated alterations in type I fiber diameter (by 36%), peak force (by 29%), and Vo (by 48%) but had no significant effect on Po, Eo, or Po/Eo. These results indicate that a modest restoration of WB activity during 14 days of NWB is sufficient to attenuate type I fiber atrophy and to partially restore type I peak isometric force and Vo to WB levels. However, the NWB-induced reductions in Po and Eo, which we hypothesize to be due to a decline in the number and stiffness of cross bridges, respectively, are considerably less responsive to this countermeasure treatment.


1997 ◽  
Vol 272 (1) ◽  
pp. R34-R42 ◽  
Author(s):  
J. J. Widrick ◽  
J. G. Romatowski ◽  
M. Karhanek ◽  
R. H. Fitts

It is well known that skeletal muscle intrinsic maximal shortening velocity is inversely related to species body mass. However, there is uncertainty regarding the relationship between the contractile properties of muscle fibers obtained from commonly studied laboratory animals and those obtained from humans. In this study we determined the contractile properties of single chemically skinned fibers prepared from rat, rhesus monkey, and human soleus and gastrocnemius muscle samples under identical experimental conditions. All fibers used for analysis expressed type I myosin heavy chain as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Allometric coefficients for type I fibers from each muscle indicated that there was little change in peak tension (force/fiber cross-sectional area) across species. In contrast, both soleus and gastrocnemius type I fiber maximal unloaded shortening velocity (Vo), the y-intercept of the force-velocity relationship (Vmax), peak power per unit fiber length, and peak power normalized for fiber length and cross-sectional area were all inversely related to species body mass. The present allometric coefficients for soleus fiber Vo (-0.18) and Vmax (-0.11) are in good agreement with published values for soleus fibers obtained from common laboratory and domesticated mammals. Taken together, these observations suggest that the Vo of slow fibers from quadrupeds and humans scale similarly and can be described by the same quantitative relationships. These findings have implications in the design and interpretation of experiments, especially those that use small laboratory mammals as a model of human muscle function.


2021 ◽  
Vol 10 (12) ◽  
pp. 2721
Author(s):  
Nobuto Nakanishi ◽  
Shigeaki Inoue ◽  
Rie Tsutsumi ◽  
Yusuke Akimoto ◽  
Yuko Ono ◽  
...  

Ultrasound has become widely used as a means to measure the rectus femoris muscle in the acute and chronic phases of critical illness. Despite its noninvasiveness and accessibility, its accuracy highly depends on the skills of the technician. However, few ultrasound phantoms for the confirmation of its accuracy or to improve technical skills exist. In this study, the authors created a novel phantom model and used it for investigating the accuracy of measurements and for training. Study 1 investigated how various conditions affect ultrasound measurements such as thickness, cross-sectional area, and echogenicity. Study 2 investigated if the phantom can be used for the training of various health care providers in vitro and in vivo. Study 1 showed that thickness, cross-sectional area, and echogenicity were affected by probe compression strength, probe angle, phantom compression, and varying equipment. Study 2 in vitro showed that using the phantom for training improved the accuracy of the measurements taken within the phantom, and Study 2 in vivo showed the phantom training had a short-term effect on improving the measurement accuracy in a human volunteer. The new ultrasound phantom model revealed that various conditions affected ultrasound measurements, and phantom training improved the measurement accuracy.


2002 ◽  
Vol 7 (2) ◽  
pp. 247-251 ◽  
Author(s):  
Masahiko Noguchi ◽  
Toshiya Kitaura ◽  
Kazuya Ikoma ◽  
Yoshiaki Kusaka

1997 ◽  
Vol 82 (3) ◽  
pp. 954-958 ◽  
Author(s):  
R. W. Mitchell ◽  
E. Rühlmann ◽  
H. Magnussen ◽  
N. M. Muñoz ◽  
A. R. Leff ◽  
...  

Mitchell, R. W., E. Rühlmann, H. Magnussen, N. M. Muñoz, A. R. Leff, and K. F. Rabe. Conservation of bronchiolar wall area during constriction and dilation of human airways. J. Appl. Physiol. 82(3): 954–958, 1997.—We assessed the effect of smooth muscle contraction and relaxation on airway lumen subtended by the internal perimeter ( A i) and total cross-sectional area ( A o) of human bronchial explants in the absence of the potential lung tethering forces of alveolar tissue to test the hypothesis that bronchoconstriction results in a comparable change of A iand A o. Luminal area (i.e., A i) and A owere measured by using computerized videomicrometry, and bronchial wall area was calculated accordingly. Images on videotape were captured; areas were outlined, and data were expressed as internal pixel number by using imaging software. Bronchial rings were dissected in 1.0- to 1.5-mm sections from macroscopically unaffected areas of lungs from patients undergoing resection for carcinoma, placed in microplate wells containing buffered saline, and allowed to equilibrate for 1 h. Baseline, A o[5.21 ± 0.354 (SE) mm2], and A i(0.604 ± 0.057 mm2) were measured before contraction of the airway smooth muscle (ASM) with carbachol. Mean A inarrowed by 0.257 ± 0.052 mm2in response to 10 μM carbachol ( P = 0.001 vs. baseline). Similarly, A onarrowed by 0.272 ± 0.110 mm2in response to carbachol ( P = 0.038 vs. baseline; P = 0.849 vs. change in A i). Similar parallel changes in cross-sectional area for A iand A owere observed for relaxation of ASM from inherent tone of other bronchial rings in response to 10 μM isoproterenol. We demonstrate a unique characteristic of human ASM; i.e., both luminal and total cross-sectional area of human airways change similarly on contraction and relaxation in vitro, resulting in a conservation of bronchiolar wall area with bronchoconstriction and dilation.


2010 ◽  
Vol 298 (1) ◽  
pp. C149-C162 ◽  
Author(s):  
Nadège Zanou ◽  
Georges Shapovalov ◽  
Magali Louis ◽  
Nicolas Tajeddine ◽  
Chiara Gallo ◽  
...  

Skeletal muscle contraction is reputed not to depend on extracellular Ca2+. Indeed, stricto sensu , excitation-contraction coupling does not necessitate entry of Ca2+. However, we previously observed that, during sustained activity (repeated contractions), entry of Ca2+is needed to maintain force production. In the present study, we evaluated the possible involvement of the canonical transient receptor potential (TRPC)1 ion channel in this entry of Ca2+and investigated its possible role in muscle function. Patch-clamp experiments reveal the presence of a small-conductance channel (13 pS) that is completely lost in adult fibers from TRPC1−/−mice. The influx of Ca2+through TRPC1 channels represents a minor part of the entry of Ca2+into muscle fibers at rest, and the activity of the channel is not store dependent. The lack of TRPC1 does not affect intracellular Ca2+concentration ([Ca2+]i) transients reached during a single isometric contraction. However, the involvement of TRPC1-related Ca2+entry is clearly emphasized in muscle fatigue. Indeed, muscles from TRPC1−/−mice stimulated repeatedly progressively display lower [Ca2+]itransients than those observed in TRPC1+/+fibers, and they also present an accentuated progressive loss of force. Interestingly, muscles from TRPC1−/−mice display a smaller fiber cross-sectional area, generate less force per cross-sectional area, and contain less myofibrillar proteins than their controls. They do not present other signs of myopathy. In agreement with in vitro experiments, TRPC1−/−mice present an important decrease of endurance of physical activity. We conclude that TRPC1 ion channels modulate the entry of Ca2+during repeated contractions and help muscles to maintain their force during sustained repeated contractions.


2001 ◽  
Vol 137 (3) ◽  
pp. 337-349 ◽  
Author(s):  
N. AHMAD ◽  
D. WILMAN

Dried lucerne (Medicago sativa), dried Italian ryegrass (Lolium multiflorum) and wheat (Triticum aestivum) straw, in the latter case supplemented with soyabean meal, were each fed to cattle, sheep and rabbits in each of 2 years. In both years, plant parts of the three diets were tested for in vitro digestibility, in both milled and chopped (1 cm lengths) form, and for cell wall content (as NDF). In the first year, the plant parts were analysed for lignin and both the plant parts and the faeces were examined microscopically for the proportions of thick-walled, thin-walled and epidermal cells in cross-sectional area and for the thickness of the cell walls.The plant parts with the lowest proportion of thick-walled cells in cross-sectional area (0·05) were the lucerne leaflets and those with the highest proportion of thick-walled cells (0·68) were the stems of wheat straw. The cell walls of the thick-walled tissues were thinnest (0·7–0·8 μm) in Italian ryegrass leaf blades and sheaths. Within each cell type for the whole crop, the order of cell wall thickness was wheat straw > lucerne > Italian ryegrass. In vitro digestibility of DM was lower (by 0·031–0·085 g digestible DM/g total DM) in chopped than in milled stems of lucerne, ryegrass and wheat and in leaf sheaths of wheat. This suggests incomplete and/or delayed access of rumen microorganisms to some of the cell wall in chopped material in vitro and probably, therefore, also in chewed material in vivo. The concentrations of NDF and lignin in both ryegrass and wheat were in the order leaf blades < leaf sheaths < stems.The lucerne crops were more mature than the ryegrass crops and there was no consistent difference between lucerne and ryegrass in intake of DM or intake of NDF. The intake of wheat straw DM was 0·52 that of lucerne and ryegrass, whereas the intake of straw NDF was 0·89 that of lucerne or ryegrass NDF. Intake of both DM and NDF in relation to metabolic body weight was highest (87–93 g DM and 45–48 g NDF/kg W0·75) with cattle on lucerne and ryegrass and rabbits on ryegrass and lowest (33–34 g DM and 29–30 g NDF/kg W0·75) with sheep and rabbits on straw. The output of faeces/kg W0·75 was particularly high (38–41 g DM and 30–32 g NDF) from rabbits fed lucerne or ryegrass. Digestibility of DM was highest (0·726–0·732 g/g) with cattle and sheep fed ryegrass, followed by cattle and sheep fed lucerne and sheep fed straw. Digestibility of NDF was highest (0·708–0·752 g digestible NDF/g total NDF) with cattle and sheep fed ryegrass and sheep fed straw. Digestibility of NDF with rabbits was lower than with cattle or sheep, but was higher than might have been expected, in a small, hind-gut fermenter, with ryegrass (0·339 g/g) and straw (0·492 g/g).The proportion of thin-walled cells was much lower in the faeces than in the diets, but there was an appreciable proportion (0·10–0·27) of these cells in the cross-sectional area of faecal particles. The cell walls of all cell types were thinner in the faeces than in the diets, e.g. those of the thick-walled cells were thinner by 0·35 μm in lucerne, by 0·11 μm in Italian ryegrass and by 0·41 μm in wheat straw. The faeces from rabbits had higher proportions of thick-walled and epidermal plant cells in cross-sectional area, and a lower proportion of thin-walled cells, than the faeces from cattle and sheep.


Heart ◽  
2020 ◽  
Vol 106 (15) ◽  
pp. 1176-1182
Author(s):  
Timothy C Tan ◽  
Maria Carmo Pereira Nunes ◽  
Mark Handschumacher ◽  
Octavio Pontes-Neto ◽  
Yong-Hyun Park ◽  
...  

ObjectiveCardioembolic (CE) stroke carries significant morbidity and mortality. Left atrial (LA) size has been associated with CE risk. We hypothesised that differential LA remodelling impacts on pathophysiological mechanism of major CE strokes.MethodsA cohort of consecutive patients hospitalised with ischaemic stroke, classified into CE versus non-CE strokes using the Causative Classification System for Ischaemic Stroke were enrolled. LA shape and remodelling was characterised by assessing differences in maximal LA cross-sectional area (LA-CSA) in a cohort of 40 prospectively recruited patients with ischaemic stroke using three-dimensional (3D) echocardiography. Flow velocity profiles were measured in spherical versus ellipsoidal in vitro models to determine if LA shape influences flow dynamics. Two-dimensional (2D) LA-CSA was subsequently derived from standard echocardiographic views and compared with 3D LA-CSA.ResultsA total of 1023 patients with ischaemic stroke were included, 230 (22.5%) of them were classified as major CE. The mean age was 68±16 years, and 464 (45%) were women. The 2D calculated LA-CSA correlated strongly with the LA-CSA measured by 3D in both end-systole and end-diastole. In vitro flow models showed shape-related differences in mid-level flow velocity profiles. Increased LA-CSA was associated with major CE stroke (adjusted relative risk 1.10, 95% CI 1.04 to 1.16; p<0.001), independent of age, gender, atrial fibrillation, left ventricular ejection fraction and CHA2DS2-VASc score. Specifically, the inclusion of LA-CSA in a model with traditional risk factors for CE stroke resulted in significant improvement in model performance with the net reclassification improvement of 0.346 (95% CI 0.189 to 0.501; p=0.00001) and the integrated discrimination improvement of 0.013 (95% CI 0.003 to 0.024; p=0.0119).ConclusionsLA-CSA is a marker of adverse LA shape associated with CE stroke, reflecting importance of differential LA remodelling, not simply LA size, in the mechanism of CE risk.


1988 ◽  
Vol 65 (3) ◽  
pp. 1231-1237 ◽  
Author(s):  
E. O. Hauschka ◽  
R. R. Roy ◽  
V. R. Edgerton

The morphological and histochemical properties of the rat soleus were studied after 1 wk of hindlimb suspension, one model that removes the weight-bearing function of the hindlimbs. To examine the effectiveness of weight support activity in maintaining soleus mass, fiber size, and succinate dehydrogenase (SDH) activity, the hindlimbs of adult male Sprague-Dawley rats were suspended (HS) and half of these rats were walked on a treadmill for 40 min/day (10 min every 6 h) at 5 m/min and a 19 degree grade (HS-WS). Significant reductions in soleus mass and fiber size were found after 1 wk of HS. Weight support activity decreased the atrophic response by approximately 50%. In the alkaline myofibrillar adenosine triphosphatase (ATPase) dark-staining fibers, SDH activity was higher in the HS than control rats, whereas it was similar to control in the HS-WS rats. Total SDH activity (SDH activity X cross-sectional area) in fibers staining lightly for ATPase in HS and HS-WS rats was lower than in control rats, whereas in the darkly stained ATPase fibers it was similar among the three groups. No changes were observed in fiber type percentages after 1 wk of HS or HS-WS. The results suggest that short-duration, daily weight support activity can ameliorate, but not prevent, soleus atrophy induced by HS. Furthermore, fiber cross-sectional area is more responsive to periodic weight support in dark than light ATPase fibers. These results also demonstrate that muscle fiber atrophy need not be associated with a loss in SDH activity.


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