Mechanical properties of human patellar tendon at the hierarchical levels of tendon and fibril

2012 ◽  
Vol 112 (3) ◽  
pp. 419-426 ◽  
Author(s):  
René B. Svensson ◽  
Philip Hansen ◽  
Tue Hassenkam ◽  
Bjarki T. Haraldsson ◽  
Per Aagaard ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Young’S Modulus ◽  
Patellar Tendon ◽  
Young's Modulus ◽  
Hierarchical Structures ◽  
Collagen Fibrils ◽  
Cross Sectional ◽  
Hierarchical Levels

Tendons are strong hierarchical structures, but how tensile forces are transmitted between different levels remains incompletely understood. Collagen fibrils are thought to be primary determinants of whole tendon properties, and therefore we hypothesized that the whole human patellar tendon and its distinct collagen fibrils would display similar mechanical properties. Human patellar tendons ( n = 5) were mechanically tested in vivo by ultrasonography. Biopsies were obtained from each tendon, and individual collagen fibrils were dissected and tested mechanically by atomic force microscopy. The Young's modulus was 2.0 ± 0.5 GPa, and the toe region reached 3.3 ± 1.9% strain in whole patellar tendons. Based on dry cross-sectional area, the Young's modulus of isolated collagen fibrils was 2.8 ± 0.3 GPa, and the toe region reached 0.86 ± 0.08% strain. The measured fibril modulus was insufficient to account for the modulus of the tendon in vivo when fibril content in the tendon was accounted for. Thus, our original hypothesis was not supported, although the in vitro fibril modulus corresponded well with reported in vitro tendon values. This correspondence together with the fibril modulus not being greater than that of tendon supports that fibrillar rather than interfibrillar properties govern the subfailure tendon response, making the fibrillar level a meaningful target of intervention. The lower modulus found in vitro suggests a possible adverse effect of removing the tissue from its natural environment. In addition to the primary work comparing the two hierarchical levels, we also verified the existence of viscoelastic behavior in isolated human collagen fibrils.

Mechanical Properties and Microarchitecture of Nanoporous Hydroxyapatite Bioceramic Nanoparticle Coatings on Ti and TiN

2006 ◽  
Vol 975 ◽  
Author(s):  
Andrei Stanishevsky ◽  
Shafiul Chowdhury ◽  
Nathaniel Greenstein ◽  
Helene Yockell-Lelievre ◽  
Jari Koskinen
Keyword(s):  
Mechanical Properties ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Biological Response ◽  
Dip Coating ◽  
Nano Crystalline ◽  
Ceramic Manufacturing ◽  
Nanoparticle Coatings

ABSTRACTThe hydroxyapatite (HA) based bioceramic materials are usually prepared at high sintering temperatures to attain suitable mechanical properties. The sintering process usually results in a material which is compositionally and morphologically different from nonstoichiometric nano-crystalline HA phase of hard tissue. At the same time, HA particulates used as precursors in ceramic manufacturing are often very similar to the natural HA nanocrystals. It has been shown that synthetic nanoparticle HA (nanoHA) based materials improve the biological response in vitro and in vivo, but the information on mechanical properties of these materials is scarce.In this work we studied the HA nanoparticle (10 – 80 nm mean size) coatings with 30 – 70% porosity prepared by a dip-coating technique on Ti and TiN substrates. It has been found that the mechanical properties of HA nanoparticle coatings are strongly influenced by the initial size, morphology, and surface treatment of nanoparticles. The nanoindentation Young's modulus and hardness of as–deposited nanoHA coatings were in the range of 2.5 – 6.9 GPa and 80 – 230 MPa, respectively. The coatings were stable after annealing up to at least 600 °C, reaching the Young's modulus up to 23 GPa and hardness up to 540 MPa, as well as in simulated body fluids.

Effects of Cyclic Stress on the Mechanical Properties of Cultured Collagen Fascicles from the Rabbit Patellar Tendon

2003 ◽  
Vol 125 (6) ◽  
pp. 893-901 ◽  
Author(s):  
Ei Yamamoto ◽  
Susumu Tokura ◽  
Kozaburo Hayashi
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Patellar Tendon ◽  
Control Level ◽  
Quadratic Function ◽  
Peak Stress ◽  
Cyclic Stress ◽  
Original Strength

Effects of cyclic stress on the mechanical properties of collagen fascicles were studied by in vitro tissue culture experiments. Collagen fascicles (approximately 300 μm in diameter) obtained from the rabbit patellar tendon were applied cyclic load at 4 Hz for one hour per day during culture period for one or two weeks, and then their mechanical properties were determined using a micro-tensile tester. There was a statistically significant correlation between tensile strength and applied peak stress in the range of 0 to 5 MPa, and the relation was expressed by a quadratic function. The maximum strength (19.4 MPa) was obtained at the applied peak stress of 1.8 MPa. The tensile strength of fascicles were within a range of control values, if they were cultured under peak stresses between 1.1 and 2.6 MPa. Similar results were also observed in the tangent modulus, which was maintained at control level under applied peak stresses between 0.9 and 2.8 MPa. The stress of 0.9 to 1.1 MPa is equivalent to approximately 40% of the in vivo peak stress which is developed in the intact rabbit patellar tendon by running, whereas that of 2.6 to 2.8 MPa corresponds to approximately 120% of the in vivo peak stress. Therefore, the fascicles cultured under applied peak stresses of lower than 40% and higher than 120% of the in vivo peak stress do not keep the original strength and modulus. These results indicate that the mechanical properties of cultured collagen fascicles strongly depend upon the magnitude of the stress applied during culture, which are similar to our previous results observed in stress-shielded and overstressed patellar tendons in vivo.

Effects of cross-sessional area and aspect ratio coupled with orientation on mechanical properties and deformation behavior of Cu nanowires

2021 ◽  
Author(s):  
Hui Cao ◽  
Wenke Chen ◽  
Zhiyuan Rui ◽  
Changfeng Yan
Keyword(s):  
Mechanical Properties ◽  
Aspect Ratio ◽  
Yield Stress ◽  
Young’S Modulus ◽  
Young's Modulus ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Cu Nanowires ◽  
The Cross

Abstract Metal nanomaterials exhibit excellent mechanical properties compared with corresponding bulk materials and have potential applications in various areas. Despite a number of studies of the size effect on Cu nanowires mechanical properties with square cross-sectional, investigations of them in rectangular cross-sectional with various sizes at constant volume are rare, and lack of multifactor coupling effect on mechanical properties and quantitative investigation. In this work, the dependence of mechanical properties and deformation mechanisms of Cu nanowires/nanoplates under tension on cross-sessional area, aspect ratio of cross-sectional coupled with orientation were investigated using molecular dynamics simulations and the semi-empirical expressions related to mechanical properties were proposed. The simulation results show that the Young’s modulus and the yield stress sharply increase with the aspect ratio except for the <110>{110}{001} Cu nanowires/nanoplates at the same cross-sectional area. And the Young’s modulus increases while the yield stress decreases with the cross-sectional area of Cu nanowires. However, both of them increase with the cross-sectional area of Cu nanoplates. Besides, the Young’s modulus increases with the cross-sectional area at all the orientations. The yield stress shows a mildly downward trend except for the <111> Cu nanowires with increased cross-sectional area. For the Cu nanowires with a small cross-sectional area, the surface force increases with the aspect ratio. In contrast, it decreases with the aspect ratio increase at a large cross-sectional area. At the cross-sectional area of 13.068 nm2, the surface force decreases with the aspect ratio of the <110> Cu nanowires while it increases at other orientations. The surface force is a linearly decreasing function of the cross-sectional area at different orientations. Quantitative studies show that Young’s modulus and yield stress to the aspect ratio of the Cu nanowires satisfy exponent relationship. In addition, the main deformation mechanism of Cu nanowires is the nucleation and propagation of partial dislocations while it is the twinning-dominated reorientation for Cu nanoplates.

scholarly journals Comparative investigations of in vitro and in vivo bioactivity of titanium vs. Ti–24Nb–4Zr–8Sn alloy before and after sandblasting and acid etching

RSC Advances ◽  
2020 ◽  
Vol 10 (40) ◽  
pp. 23582-23591
Author(s):  
Xin Liu ◽  
Yumei Niu ◽  
Weili Xie ◽  
Daqing Wei ◽  
Qing Du
Keyword(s):  
Young’S Modulus ◽  
Young's Modulus ◽  
Stress Shielding ◽  
Acid Etching ◽  
Implant Material ◽  
Before And After ◽  
New Type

To avoid the failure of clinical surgery due to “stress shielding” and the loosening of an implant, a new type of alloy, Ti–24Nb–4Zr–8Sn (TNZS), with a low Young's modulus acted as a new implant material in this work.

Role of pH on stability and mechanical properties of gelatin films

2012 ◽  
Vol 27 (1) ◽  
pp. 67-77 ◽  
Author(s):  
Michela Gioffrè ◽  
Paola Torricelli ◽  
Silvia Panzavolta ◽  
Katia Rubini ◽  
Adriana Bigi
Keyword(s):  
Mechanical Properties ◽  
Young’S Modulus ◽  
Triple Helix ◽  
Young's Modulus ◽  
In Vitro Tests ◽  
Diffraction Reflection ◽  
X Ray Diffraction ◽  
Solution Ph ◽  
Gelatin Films

The effect of the film-forming solution pH on the triple-helix content, thermal stability, and mechanical properties of gelatin films was investigated. The films were prepared from solutions at different pHs of type A pigskin gelatin, and their mechanical characteristics were determined. At pHs higher than 9 and lower than 5, Young’s modulus, E, and the stress at break, σb, of the films decreased significantly. Cross-linking with genipin reduced deformation at break, ϵb, and increased Young’s modulus. The intensity of the 1.1-nm X-ray diffraction reflection and the denaturation enthalpy decreased at these pHs, indicating that the triple helix reduced. Preliminary in vitro tests on the cross-linked samples indicated good cell proliferation and viability.

Effects of Static Stress on the Mechanical Properties of Cultured Collagen Fascicles From the Rabbit Patellar Tendon

2001 ◽  
Vol 124 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Ei Yamamoto ◽  
Wataru Iwanaga ◽  
Hiroshi Miyazaki ◽  
Kozaburo Hayashi
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Applied Stress ◽  
Patellar Tendon ◽  
Control Level ◽  
Peak Stress ◽  
Static Stress ◽  
Tangent Modulus

In-vitro tissue culture experiments were performed to study the effects of static stress on the mechanical properties of collagen fascicles obtained from the rabbit patellar tendon. After collagen fascicles having the diameter of approximately 300 μm were cultured for 1 and 2 wk under static stress between 0 and 3 MPa, their mechanical properties and crimp morphology were determined using a micro-tensile tester and a light microscope, respectively. The tensile strength and tangent modulus of the fascicles were significantly decreased by culture under no load compared to control fascicles. A statistically significant correlation, which was described by a quadratic curve, was observed between applied stress and tensile strength. The maximum tensile strength (16.7 MPa) was obtained at the applied stress of 1.2 MPa; the strength was within a range of control values. There was a similar correlation between applied stress and tangent modulus, and the modulus was maintained at control level under 1.3 MPa stress. The stress of 1.2 to 1.3 MPa is equivalent to approximately 50 percent of the peak stress developed in the intact rabbit patellar tendon by running. Strain at failure of cultured collagen fascicles was negatively correlated with applied stress, and that at 1.2 to 1.3 MPa stress was almost the same as the control value. Crimp morphology in the fascicles cultured under about 1.2 MPa stress was similar to that in control fascicles. These results indicate that cultured collagen fascicles change the mechanical properties and structure in response to static tensile stress. In addition, their mechanical properties and structure are maintained at control level if the static stress of 50 percent of in-vivo peak stress is applied.

scholarly journals Modifications of the mechanical properties of in vivo tissue-engineered vascular grafts by chemical treatments for a short duration

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248346
Author(s):  
Tomoya Inoue ◽  
Keiichi Kanda ◽  
Masashi Yamanami ◽  
Daisuke Kami ◽  
Satoshi Gojo ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Young’S Modulus ◽  
Short Duration ◽  
Young's Modulus ◽  
Vascular Grafts ◽  
Ultimate Strain ◽  
Burst Pressure ◽  
Chemical Treatments ◽  
Retention Strength

In vivo tissue-engineered vascular grafts constructed in the subcutaneous spaces of graft recipients have functioned well clinically. Because the formation of vascular graft tissues depends on several recipient conditions, chemical pretreatments, such as dehydration by ethanol (ET) or crosslinking by glutaraldehyde (GA), have been attempted to improve the initial mechanical durability of the tissues. Here, we compared the effects of short-duration (10 min) chemical treatments on the mechanical properties of tissues. Tubular tissues (internal diameter, 5 mm) constructed in the subcutaneous tissues of beagle dogs (4 weeks, n = 3), were classified into three groups: raw tissue without any treatment (RAW), tissue dehydrated with 70% ET (ET), and tissue crosslinked with 0.6% GA (GA). Five mechanical parameters were measured: burst pressure, suture retention strength, ultimate tensile strength (UTS), ultimate strain (%), and Young’s modulus. The tissues were also autologously re-embedded into the subcutaneous spaces of the same dogs for 4 weeks (n = 2) for the evaluation of histological responses. The burst pressure of the RAW group (1275.9 ± 254.0 mm Hg) was significantly lower than those of ET (2115.1 ± 262.2 mm Hg, p = 0.0298) and GA (2570.5 ± 282.6 mm Hg, p = 0.0017) groups. Suture retention strength, UTS or the ultimate strain did not differ significantly among the groups. Young’s modulus of the ET group was the highest (RAW: 5.41 ± 1.16 MPa, ET: 12.28 ± 2.55 MPa, GA: 7.65 ± 1.18 MPa, p = 0.0185). No significant inflammatory tissue response or evidence of residual chemical toxicity was observed in samples implanted subcutaneously for four weeks. Therefore, short-duration ET and GA treatment might improve surgical handling and the mechanical properties of in vivo tissue-engineered vascular tissues to produce ideal grafts in terms of mechanical properties without interfering with histological responses.

scholarly journals Measurements of Young's Modulus of Elasticity of the Canine Aorta with Ultrasound

1979 ◽  
Vol 1 (4) ◽  
pp. 356-367 ◽  
Author(s):  
D.J. Hughes ◽  
C.F. Babbs ◽  
L.A. Geddes ◽  
J.D. Bourland
Keyword(s):  
Young’S Modulus ◽  
Modulus Of Elasticity ◽  
Young's Modulus ◽  
Parietal Pleura ◽  
Young’S Modulus Of Elasticity ◽  
Blood Pressures ◽  
Canine Aorta

We have developed an ultrasonic technique for determining the dynamic Young's modulus of elasticity (E) of the canine aorta in vivo. Young's modulus was measured in the descending thoracic aorta (DTA) and the abdominal aorta (AA) of 12 dogs over a range of mean blood pressures from 40 – 200 mm Hg. The vessels were excised and dynamic moduli were determined in vitro post-mortem from pressure-volume curves. The data so obtained were compared to the in vivo values. In vivo and in vitro moduli increased exponentially with mean distending pressure (P). The equation of best fit for these data was of the form E = R0 exp(aP). E0 and a depend on the site of measurement (AA or DTA) and upon the particular animal. In vivo and in vitro moduli were not significantly different in the AA (AA: in vivo E0 = 667 ± 382 mm Hg, a = 0.017 ± 0.004 mm Hg-1 in vitro E0 = 888 ± 367, a = 0.016 ± 0.002). However, in vivo moduli exceeded in vitro moduli in the DTA. (DTA: in vivo E0 = 687 ± 241, a = 0.016 ± 0.004 in vitro E0 = 349 ± 64, a = 0.018 ± 0.003). The increased stiffness of the DTA compared to the AA in vivo may be due to the in situ tethering of the aorta to the spine by the parietal pleura.

Structural instability and mechanical properties of MoS2toroidal nanostructures

2015 ◽  
Vol 17 (48) ◽  
pp. 32425-32435 ◽  
Author(s):  
Jianyang Wu ◽  
Gaosheng Nie ◽  
Jun Xu ◽  
Jianying He ◽  
Qingchi Xu ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Molecular Dynamics ◽  
Tensile Strength ◽  
Young’S Modulus ◽  
Structural Stability ◽  
Md Simulation ◽  
Young's Modulus ◽  
Hierarchical Structures ◽  
Structural Instability ◽  
High Tensile Strength

Classic molecular dynamics (MD) simulation of hypothetical MoS2NT nanorings and their woven hierarchical structures shows a strong dimension-dependent structural stability, and reveals that the hierarchical structures with 4-in-1 weaves exhibit high tensile strength and Young's modulus.

Sign in / Sign up

Export Citation Format

Share Document