scholarly journals Phytochemicals and Antioxidant Capacity fromNypa fruticansWurmb. Fruit

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Nagendra Prasad ◽  
Bao Yang ◽  
Kin Weng Kong ◽  
Hock Eng Khoo ◽  
Jian Sun ◽  
...  

Nypa fruticansWurmb. is one of the important underutilized fruit of Malaysia, which lacks scientific attention. Total phenolics, flavonoid content, and antioxidant capacities from endosperm extracts ofNypa fruticans(unripe and ripe fruits) were evaluated. Endosperm extract of unripe fruits (EEU) exhibited the highest phenolics (135.6 ± 4.5 mg GAE/g), flavonoid content (68.6 ± 3.1 RE/g), and antioxidant capacity. Free radical scavenging capacity of EEU as assessed by 2-2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radicals showed inhibitory activity of78±1.2% and85±2.6%, respectively. Beta carotene bleaching coefficient of EEU was higher (2550±123), when compared to endosperm extract of ripe fruits (1729±172). Additionally, EEU exhibited high antioxidant capacity by phosphomolybdenum method and ferric reducing antioxidant power values. Eight phenolic compounds fromNypa fruticansendosperm extracts were identified and quantified by ultra-high-performance liquid chromatography. Chlorogenic acid, protocatechuic acid, and kaempferol were the major phenolic compounds. Thus this fruit could be used as a potential source of natural antioxidant.

Author(s):  
Nur Fatimah Lasano ◽  
Asmah Rahmat ◽  
Nurul Shazini Ramli ◽  
Mohd Fadzelly Abu Bakar

Objective: This study aimed to evaluate the effect of oven and microwave drying on total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity of unfermented and fermented tea developed from Strobilanthes crispus leaves.Methods: TPC and TFC were estimated using a spectrophotometric method, while antioxidant capacity was determined using ferric reducing antioxidant power assay and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Camellia sinensis (tea plant), that is, used for the production of all varieties of commercial tea and fresh S. crispus leaves were served as controls in this study.Results: The highest antioxidant activity and TPC were observed in S. crispus tea developed from microwave-dried leaves, while the highest TFC was observed in oven-dried tea. Unfermented S. crispus tea showed significantly higher values (p<0.05) for antioxidant activity, TPC, and TFC as compared to fermented S. crispus tea. A strong and moderate correlation was observed between antioxidant activity and TPC as well as TFC values.Conclusion: The present work clearly showed that S. crispus tea developed from microwave-dried leaves able to preserve the polyphenols and hence contribute to excellent antioxidant capacity. Incorporation of unfermented S. crispus tea in the diet can be a good source of natural antioxidant.


Botanica ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 76-87
Author(s):  
Aziza Lfitat ◽  
Hind Zejli ◽  
Abdelkamel Bousselham ◽  
Yassine El Atki ◽  
Badiaa Lyoussi ◽  
...  

AbstractWe conducted this study to determine and compare the content of phenolic compounds and flavonoids in the argan and olive leaves as well as their antioxidant capacity in aqueous, methanolic, and ethyl acetate extracted fractions. In vitro antioxidant activity was evaluated in comparison with synthetic antioxidants by assessing DPPH• radical scavenging capacity, ferric reducing antioxidant power, scavenging ability by inhibiting the β-carotene/linoleic acid emulsion oxidation, and by the ABTS radical scavenging activity assay. Total phenolic content in argan samples ranged from 221.69 ± 2.07 to 1.32 ± 0.01 mg GAE/g DW and in olive samples from 144.61 ± 0.82 to 1.21 ± 0.02 mg GAE/g DW. Total flavonoids content in argan samples varied from 267.37 ± 1.12 to 25.48 ± 0.02 mg QE/g DW, while in olives from 96.06 ± 0.78 to 10.63 ± 0.05 mg QE/g DW. In vitro antioxidant studies strongly confirmed the antioxidant potency of argan and olive leaves and their richness in secondary metabolites that are effective in free radicals scavenging and metal chelating capacities, indicating their antioxidant power.


2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Marieta Hristozkova ◽  
Liliana Gigova ◽  
Maria Geneva ◽  
Ira Stancheva ◽  
Ivanina Vasileva ◽  
...  

Abstract Mycorrhizal fungi, algae and cyanobacteria are some of the most important soil microorganisms and major components of a sustainable soil-plant system. This study presents for the first time evidence of the impact of green alga and cyanobacterium solely and in combination with arbuscular mycorrhizal fungi (AMF) on plant-antioxidant capacity. In order to provide a better understanding of the impact of AMF and soil microalgae on Ocimum basilicum L. performance, changes in the pattern and activity of the main antioxidant enzymes (AOEs), esterases and non-enzymatic antioxidants including phenols, flavonoids, ascorbate, and α-tocopherols were evaluated. The targeted inoculation of O. basilicum with AMF or algae (alone and in combination) enhanced the antioxidant capacity of the plants and the degree of stimulation varied depending on the treatment. Plants in symbiosis with AMF exhibited the highest antioxidant potential as was indicated by the enhanced functions of all studied leaf AOEs: 1.5-, 2- and more than 10-fold rises of superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione reductase (GR), respectively. The greatest increase in the total esterase activity and concentration of phenols, flavonoids and ascorbate was marked in the plants with simultaneous inoculation of mycorrhizal fungi and the green algae. 2,2-diphenyl-1-pycril-hydrazyl (DPPH) free radical scavenging method and ferric reducing antioxidant power (FRAP) assay proved the increased plant antioxidant capacity after co-colonization of green algae and mycorrhizae.


Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1276
Author(s):  
Ding-Tao Wu ◽  
Xi-Rui Nie ◽  
Dan-Dan Shen ◽  
Hong-Yi Li ◽  
Li Zhao ◽  
...  

In this study, the phenolic profiles and bioactivities of five representative cultivars of okra collected in China were investigated. Noticeable variations of phenolic compounds and their bioactivities were observed among these different cultivars of okra. The contents of total flavonoids (TFC) in “Shuiguo”, “Kalong 8”, “Kalong 3”, “Wufu”, and “Royal red” ranged from 1.75 to 3.39 mg RE/g DW, of which “Shuiguo” showed the highest TFC. Moreover, five individual phenolic compounds were found in okra by high performance liquid chromatography analysis, including isoquercitrin, protocatechuic acid, quercetin-3-O-gentiobioside, quercetin, and rutin, while isoquercitrin and quercetin-3-O-gentiobioside were detected as the main phenolic compounds in okra. Moreover, all tested okra exhibited significant antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity, 2,2’-azino-bis (3-ethylenzthiazoline-6-sulphonic acid) radical scavenging capacity, and ferric reducing antioxidant power) and inhibitory effects on digestive enzymes (lipase, α-glucosidase, and α-amylase). Indeed, “Shuiguo” exhibited much better antioxidant activities and inhibitory activities on digestive enzymes, which might be attributed to its high TFC. Results suggested that okra, especially “Shuiguo”, could be developed as natural antioxidants and inhibitors against hyperlipidemia and hyperglycemia in the fields of functional foods and pharmaceuticals, which could meet the increasing demand for high-quality okra with health-promoting properties in China.


2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Gordana Zdunic ◽  
Dejan Godjevac ◽  
Katarina Savikin ◽  
Silvana Petrovic

A comparative analysis of the phenolic compounds in the 70% EtOH extracts of Hypericum acutum, H. androsaemum, H. barbatum, H. hirsutum, H. maculatum, and H. richeri has been carried out using high-performance liquid chromatography coupled with photodiode array UV detection and high resolution TOF mass spectrometry. Quercetin, astilbin, I3, II8-biapigenin, orientin, 2”- O-acetylorientin, three phenolcarboxylic acids, and eight flavonols 3- O-glycosides were identified in the extracts on the basis of their on-line UV spectra, accurate mass spectral data, and in comparison of retention times with those from the standards. Fingerprint analysis of the extracts revealed significant differences in the qualitative and quantitative chemical composition of the studied species. Antioxidant assays with various reaction mechanisms were used including ferric reducing antioxidant power (FRAP) assay, DPPH, ABTS, superoxide anion radical scavenging capacity and inhibition of liposome peroxidation induced by Fe2+. The most potent were extracts of H. acutum and H. maculatum indicating this Hypericum species interesting for further research aimed as a potentially new source of biologically active compounds.


2013 ◽  
Vol 76 (7) ◽  
pp. 1218-1225 ◽  
Author(s):  
YOLANDA RUIZ-NAVAJAS ◽  
MANUEL VIUDA-MARTOS ◽  
ESTHER SENDRA ◽  
JOSÉ A. PEREZ-ALVAREZ ◽  
JUANA FERNÁNDEZ-LÓPEZ

The aim of this work was to determine (i) the antioxidant capacity of Thymus moroderi, Thymus piperella, Santolina chamaecyparissus, and Sideritis angustifolia essential oils (EOs) by means of four different antioxidant tests (the 2,2′-diphenyl-1-picrylhydrazyl radical scavenging method, the ferrous ion–chelating ability assay, the ferric reducing antioxidant power test, and the thiobarbituric acid reactive species test) and (ii) the antifungal activity against molds and yeast of these EOs by the agar dilution and the microdilution methods. T. piperella EO showed the lowest 50% inhibitory concentration by the 2,2′-diphenyl-1-picrylhydrazyl radical scavenging method (9.30 mg/ml) and by the thiobarbituric acid reactive species test (6.30 mg/ml) and the highest value by the ferric reducing antioxidant power test (2.64 Trolox equivalent antioxidant capacity), while S. chamaecyparissus showed the lowest 50% inhibitory concentration in the ferrous ion–chelating ability assay (3.94 mg/ml). All EOs had a substantial inhibitory effect on all assayed yeast strains. S. angustifolia EO had the lowest MICs (2.5 μl/ml) for the yeasts Saccharomyces cerevisiae, Debaryomyces hansenii, Rhodotorula mucilaginosa, and Pichia carsonii. As regards antifungal activity, S. angustifolia EO at high concentrations was the most effective EO in reducing the growth of Alternaria alternata, Penicillium chrysogenum, and Mucor racemosus, while S. chamaecyparissus was the best inhibitor of the molds Aspergillus flavus and Mucor circinelloides. The results obtained in this study suggest the possibility of using these essential oils as natural antioxidant food preservatives.


Author(s):  
Ciliana Flórez-Montes ◽  
Óscar Marino Mosquera-Martínez ◽  
Andrés Felipe Rojas-González

Currently, the use of agro-industrial waste represents a viable alternative for obtaining valuable compounds that, in the case of biorefineries, is an opportunity for improvement. In this context, the aim of our study was to determine the flavonoid content and the antioxidant capacity of 30 byproducts from fruit processing in Colombia and propose possible applications. We analyzed the antioxidant capacity of the ethanolic extracts from each byproduct using the ferric reducing antioxidant power (FRAP) and the reducing power of antioxidant activity (RPAA) assays, as well as the oxygen radical absorbance capacity (ORAC) test. We found that tree tomato peels and stem and peach peels had a high flavonoid content, with values greater than 8,271.82 ± 702.70 μg quercetin per gram of dry sample. We also found that mango (22,676.57 ± 759.71 μg TE1/g Sdb, 3,692.38 ± 92.67 μg GAE/g Sdb), soursop (22,117.13 ± 754.94 μg TE1/g Sdb, 4,858.79 ± 156.71 μg GAE/g Sdb, 14,713.39 ± 757.95 μg TE2/g Sdb), grape (17,027.85 ± 765.11 μg TE1/g Sdb, 13,395.15 ± 659.31 μg TE2/g Sdb), peach peels (17,910.21 ± 1,424.33 μg TE2/g Sdb) and seeds (4,316.46 ± 112.00 μg GAE/g Sdb, 20,093.32 ± 1,317.93 μg TE2/g Sdb), and grape stalk (3,552.26 ± 31.63 μg GAE/g Sdb) showed a high antioxidant capacity in the different tests performed. Our results demonstrate that fruit byproducts have potential use in the pharmaceutical,  ood, and cosmetic industry due to  heir flavonoids content and their high antioxidant capacity.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Sathsara T. Deyalage ◽  
Indira Wickramasinghe ◽  
Nimesha Amarasinghe ◽  
Gayan Thilakarathna

Green leafy vegetables (GLVs) are abundant in bioactive compounds and constitute a crucial part of a balanced diet. Sri Lankan green leafy vegetables which are edible and available for consumption have not been thoroughly investigated, whilst their consumption can deflate the risk of arising several degenerative diseases, such as cancer and cardiovascular diseases. The present study was aimed at evaluating the antioxidant capacities of the leafy vegetables, Gymnema lactiferum (Kurignan), Wattakaka volubilis (Aguna), and Argyreia populifolia (Girithilla), with different thermal treatments (70°C, 120°C, and 170°C) which are used in domestic cooking processes. Heat treatments significantly affect the antioxidant capacity and polyphenolic content of most vegetables analyzed, either increasing or decreasing the concentration of these compounds. In the presence of thermal treatments, Gymnema lactiferum (14.52-20.28 mg GAE/g DW) and Wattakaka volubilis (19.75-27.13 mg GAE/g DW) showed a significantly higher ( p < 0.05 ) total phenolic content. The temperature treatment did not alter the ABTS radical scavenging activity in Gymnema lactiferum. In contrast, an increment of ferric reducing antioxidant power (41.26-54.85 mg TE/g DW) and DPPH radical scavenging activity (0.11-0.26 mg TE/g DW) was observed. Wattakaka volubilis treated at 170°C appeared to have a significantly higher amount ( 104.93 ± 1.41   mg TE/g DW) of ferric reducing antioxidant power compared to its raw form. All cooking processes with their corresponding thermal treatments caused a significantly lower ( p < 0 ) amount of antioxidant capacity in Argyreia populifolia.


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Ruth Edwige Kemadjou Dibacto ◽  
Boris Ronald Tonou Tchuente ◽  
Maxwell Wandji Nguedjo ◽  
Yves Martial Tongue Tientcheu ◽  
Emilienne Carine Nyobe ◽  
...  

Fruit peels are increasingly being used as functional foods nowadays. Peelings of twelve varieties of Persea americana fruits consumed in Cameroon were investigated for their phenolic compounds (polyphenols and flavonoids) using three solvents systems, water, ethanol: water (50 : 50 v / v ), and ethanol, and antioxidant activity using total antioxidant capacity (TAC), ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging methods. Total polyphenol, flavonoids, and antioxidant potential of the peels significantly varied with P. americana variety and also with the extraction solvents in the order ethanol > ethanol: water > water. Total phenolic content varied from 2407 (Fuerte florid) to 673 (Semil) mg GAE/g DM, respectively, while flavonoids varied from 986 to 119 mg QE/g DM for Fuerte florid and Hickson varieties, respectively. TAC, respectively, varied between 132.87 and 126.85 mg AAE/g DM with Hass and Semil varieties, respectively. The highest DPPH scavenging capacity was recorded for the ethanolic extract with Lula (86.33%) and the least for the aqueous extract with the Semil (30.11%) variety. With FRAP, the highest capacity was obtained with hydroethanolic extract of Fuerte florid (0.43 mg AAE/g DM) and the least for aqueous extract with the Semil (0.269 mg AAE/g DM) variety. In conclusion, varieties of avocado peels are a good source of antioxidants. Solvent extraction significantly affected the concentration of bioactive compounds but not the potency of the antioxidants. A weakly positive correlation but not significant between the quantity of polyphenol, flavonoid, and antioxidant capacity of avocado peelings was obtained in this study.


2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1985625 ◽  
Author(s):  
Ady Giordano ◽  
Gonzalo Fuentes-Barros ◽  
Sebastián Castro-Saavedra ◽  
Antonia González-Cooper ◽  
Cristian Suárez-Rozas ◽  
...  

Cryptocarya alba is an important tree species in the Chilean sclerophyllous forest. Its leaves and bark are used in traditional medicine to treat liver diseases and rheumatism. Analyses of the essential oil (EO) show serious discrepancies, and information on other constituents is limited. The aerial biomass of individual trees from 3 wild populations, some old trees, and farmed saplings were analyzed ( n = 132). The EO profiles were studied by gas chromatography/mass spectrometry (GC/MS). The alkaloidal and polyphenol compositions were determined by ultra-high-performance liquid chromatography (UHPLC)/MS-MS. The total polyphenol content, the total flavonoid content, and the antioxidant capacity (diphenylpicrylhydrazyl, azinobisethylbenzothiazolinesulfonic acid, and ferric reducing antioxidant power: DPPH, ABTS, and FRAP respectively) were determined by standard methods. Significant differences were found at the individual and population levels in the contents of polyphenols, total flavonoids, antioxidant capacity, and specific alkaloids for leaves, bark, and wood. Farmed saplings grown under less light showed higher concentrations of higenamine, N-methylcoclaurine, N-methyllaurotetanine, and isocorydine, while those receiving more light were richer in laurolitsine, boldine, coclaurine, catechin, quercetin, epicatechin, quercitrin, and procyanidins. Important variations were found according to the season, age of the tree and of the leaves. The EO composition also varied considerably. These results support the idea that the natural variability of medicinal species is an important subject for study.


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