scholarly journals Evaluation of Green Manure Amendments for the Management of Fusarium Basal Rot (Fusarium oxysporumf.sp.cepae) on Shallot

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Assefa Sintayehu ◽  
Seid Ahmed ◽  
Chemeda Fininsa ◽  
P. K. Sakhuja
Keyword(s):  
Green Manure ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Limiting Factors ◽  
Lepidium Sativum ◽  
Ethiopian Mustard ◽  
Basal Rot ◽  
Fusarium Basal Rot ◽  
Manure Amendments

Shallot (Allium cepaL. var.ascalonicum) is the most traditional vegetable crop in Ethiopia. Shallot is susceptible to a number of diseases that reduce yield and quality, among which fusarium basal rot (FBR) caused byFusarium oxysporumf.sp.cepae (Foc)is one of the most important yield limiting factors in Ethiopia. The present study was designed to evaluate the effectiveness ofBrassicacrops for the management of shallot FBR on shallot. The experiments were carried out at Debre Zeit Agricultural Research Center using cabbage (Brassica oleracea), garden cress (Lepidium sativum), Ethiopia mustard (B. carinata), and rapeseed (B. napus). The evaluations were done underin vitroand greenhouse conditions. Underin vitrotest condition it was confirmed that extracts of Ethiopian mustard and rapeseed showed higher inhibition on the growth ofFocpathogen compared to control. Data on seedling emergence, plant height, plant stand, disease incidence, severity, cull bulbs, and bulb weight were collected in greenhouse experiment. The green manure amendments of rapeseed and Ethiopian mustard significantly reduced disease incidence by 21% and 30% and disease severity by 23% and 29%, respectively. However the plant emergency was not significantly different among treatments in greenhouse test. These results indicated that Ethiopian mustard and rapeseed crops have potential as green manure for the management of FBR disease of shallot crop.

​Effect of Nutrients on Wilt in Chickpea

2021 ◽  
Author(s):  
B.D.S. Nathawat ◽  
O.P. Sharma ◽  
Manju Kumari ◽  
Hansraj Shivran
Keyword(s):  
Fusarium Oxysporum ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Weather Conditions ◽  
Limiting Factors ◽  
Grain Legume ◽  
Nitrogen Levels ◽  
Chickpea Cultivar ◽  
Phosphorus Application ◽  
Wilt Incidence

Background: Chickpea (Cicer arietinum L.) contributes 18% of the global production of grain legume and serves as an important source of dietary protein. Fusarium wilt, caused by soil borne fungus Fusarium oxysporum f. sp. ciceri appears to be the most devastating diseases of chickpea throughout the world. It is one of the important limiting factors of chickpea production in India. The disease causes substantial yield losses which may reach even 100 per cent under favorable weather conditions. Methods: Pot culture experiments were conducted at Rajasthan Agricultural Research Institute, Durgapura, Jaipur during rabi 2014-15 and 2015-16 to study the effect of nutrients on wilt caused by Fusarium oxysporum f. sp. ciceri (Padwick) in chickpea (Cicer arirtinum). In the first experiment, the treatments comprised of four nitrogen levels-viz. 0, 15, 20 and 25 kg/ha and four phosphorus levels- viz. 0, 30, 40 and 50 kg/ha tried in sixteen treatment combinations. Surface sterilized seeds of chickpea cultivar L-550 were sown in each pot. After sowing, 400 ml water was supplied to each pot; there after watering was done at an interval of 6 days. In second experiment, the treatments comprised of 7 micronutrients viz. zinc, manganese, calcium, copper, cobalt, iron and nickel used at the rate of 10mg/kg of soil. Chickpea cultivar L-550 was used as a test crop. Result: Disease incidence was recorded periodically commencing 15 days after sowing and was continued up to 55 days after sowing. Levels of nitrogen application had increased wilt incidence successively in both the years. Whereas, a reverse trend was observed with the application of phosphorus, incidence of wilt was decreased with increasing levels of phosphorus. Application of zinc and calcium reduced the wilt incidence significantly over check. Zinc was found to be most effective in minimizing the disease incidence.

scholarly journals IN VITRO AND FIELD PERFORMANCE OF SOME SEED TREATING FUNGICIDAL GROUPS ON GERMINATION, DISEASE INCIDENCE AND YIELD CONTRIBUTING CHARACTERS OF CHICKPEA (BARI Chola-10)

2020 ◽  
Vol 4 (1) ◽  
pp. 45-50
Author(s):  
Md. Shahriar kobir ◽  
Md. Hafijur Rahman ◽  
Pradip Hajong ◽  
Md. Harun-Or- Rashid
Keyword(s):  
Grain Yield ◽  
Seed Treatment ◽  
Agricultural Research ◽  
Disease Incidence ◽  
Field Performance ◽  
Experimental Treatment ◽  
Research Station ◽  
Vigor Index ◽  
Group Treatments

Low productivity of chickpea in Bangladesh is obtained due to different disease incidence and seed treatment by fungicides can minimize the disease severity. A laboratory and field experiment was conducted at Regional Agricultural Research Station, Jashore, Bangladesh to evaluate the effect of some seed treating fungicidal groups on germination, soil-borne as well as seed-borne disease incidence and yield contributing characters of chickpea (BARI Chola-10). In laboratory the experiment was conducted in CR design and in field condition the experiment was conducted in RCB design with three replications in both conditions. Five fungicides namely Provax 200 WP (Carboxin +Thiram), Secure 600 WG (Fenamidone + Mancozeb), Rovral 50 WP (Iprodione), Bavistin 50 WP (Carbendazim), Captan 50 WP along with control was maintained as experimental treatment. The fungicidal group treatments showed significant variation among the parameters except days to mature. The highest germination (92%), plumule length (31.22 mm), radicle length (12.26 mm), Vigor index (4015.5), Percent disease reduction over control (84%), plant height (63.67 cm), plants m- 2 (31), pods plant-1 (81), grain yield (2062.7 kg ha-1) and the lowest days to 50 % flowering (65), disease incidence (3.33%) was found when seeds were treated with fungicide Provax 200 WP (Carboxin +Thiram). To reduce the soil-borne as well as seed-borne disease incidence and to increase grain yield of chickpea, seed treatment with provax 200 WP should be followed.

scholarly journals Onion Trait Heritability and Response from Selection

2006 ◽  
Vol 131 (5) ◽  
pp. 646-650 ◽  
Author(s):  
Christopher S. Cramer
Keyword(s):  
Recurrent Selection ◽  
Disease Incidence ◽  
Heritability Estimate ◽  
Selection Response ◽  
Response Analysis ◽  
Realized Heritability ◽  
Basal Rot ◽  
Fusarium Basal Rot ◽  
Sib Families ◽  
Heritability Estimates

Realized heritability estimates of bolting percentage, pink root and fusarium basal rot severities and incidences, and percentage of single-centered bulbs were estimated for half-sib families of an intermediate-day, open-pollinated onion (Allium cepa L.) population using selection response analysis. Half-sib families were selected based upon an index that equally weighted bolting percentage, pink root and fusarium basal rot severities and incidences, percentage of single-centered bulbs, and bulb quality. Families were subjected to one cycle of half-sib family recurrent selection. Pink root and fusarium basal rot severity was reduced by 17% and 7%, respectively, with realized heritability estimates of 1.28 and 0.65, respectively. More progress for pink root severity was made than was selected. Disease incidence was reduced by 18% and 12%, respectively, with heritability estimates of 0.65 and 0.60, respectively. Very little progress was made for the percentage of single-centered bulbs and this was reflected in a heritability estimate of 0.17. Selection based upon multiple characters at the same time may reduce the effectiveness of making improvements in a single trait. However even with low to moderate heritability, improvements were made, and suggest that further improvements can be made through selection.

scholarly journals Fusarium basal rot: profile of an increasingly important disease in Allium spp.

2021 ◽  
Author(s):  
Dung Le ◽  
Kris Audenaert ◽  
Geert Haesaert
Keyword(s):  
Disease Management ◽  
Cultural Practices ◽  
Current Knowledge ◽  
Management Strategies ◽  
Future Directions ◽  
Basal Rot ◽  
Major Constraint ◽  
Fusarium Basal Rot ◽  
Chemical Pesticides ◽  
Important Disease

AbstractFusarium basal rot (FBR) is a soil-borne disease that affects Allium species worldwide. Although FBR has long been recognized as a major constraint to the production of economically important Allium species, information that could support disease management remains scattered. In this review, the current knowledge on the causal agents, symptomology and epidemiology, impact, and management strategies of FBR is synthesized. We highlight that FPR is associated with different complexes of several Fusarium species, of which Fusarium oxysporum and F. proliferatum are the most prevalent. These pathogenic complexes vary in composition and virulence, depending on sites and hosts, which can be challenging for disease management. Research to improve disease management using chemical pesticides, resistance cultivars, biocontrol agents, and cultural practices has achieved both promising results and limitations. Finally, research needs and future directions are proposed for the development of effective FBR management strategies.

scholarly journals Development of a Fluorescent Tool for Studying Legionella bozemanae Intracellular Infection

2021 ◽  
Vol 9 (2) ◽  
pp. 379
Author(s):  
Breanne M. Head ◽  
Christopher I. Graham ◽  
Teassa MacMartin ◽  
Yoav Keynan ◽  
Ann Karen C. Brassinga
Keyword(s):  
Growth Kinetics ◽  
Legionella Pneumophila ◽  
Fluorescent Protein ◽  
Disease Incidence ◽  
Acanthamoeba Castellanii ◽  
Infection Model ◽  
Intracellular Infection ◽  
Green Fluorescent

Legionnaires’ disease incidence is on the rise, with the majority of cases attributed to the intracellular pathogen, Legionella pneumophila. Nominally a parasite of protozoa, L. pneumophila can also infect alveolar macrophages when bacteria-laden aerosols enter the lungs of immunocompromised individuals. L. pneumophila pathogenesis has been well characterized; however, little is known about the >25 different Legionella spp. that can cause disease in humans. Here, we report for the first time a study demonstrating the intracellular infection of an L. bozemanae clinical isolate using approaches previously established for L. pneumophila investigations. Specifically, we report on the modification and use of a green fluorescent protein (GFP)-expressing plasmid as a tool to monitor the L. bozemanae presence in the Acanthamoeba castellanii protozoan infection model. As comparative controls, L. pneumophila strains were also transformed with the GFP-expressing plasmid. In vitro and in vivo growth kinetics of the Legionella parental and GFP-expressing strains were conducted followed by confocal microscopy. Results suggest that the metabolic burden imposed by GFP expression did not impact cell viability, as growth kinetics were similar between the GFP-expressing Legionella spp. and their parental strains. This study demonstrates that the use of a GFP-expressing plasmid can serve as a viable approach for investigating Legionella non-pneumophila spp. in real time.

scholarly journals Using Commercial Compost as Control Measures against Cucumber Root-Rot Disease

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Kamel Kamal Sabet ◽  
Magdy Mohamed Saber ◽  
Mohamed Adel-Aziz El-Naggar ◽  
Nehal Samy El-Mougy ◽  
Hatem Mohamed El-Deeb ◽  
...  
Keyword(s):  
Root Rot ◽  
Control Measure ◽  
Disease Incidence ◽  
Pathogenic Fungi ◽  
Pythium Ultimum ◽  
Control Measures ◽  
Infested Soil ◽  
Fungal Isolates ◽  
Cucumber Root

Five commercial composts were evaluated to suppress the root-rot pathogens (Fusarium solani (Mart.) App. and Wr, Pythium ultimum Trow, Rhizoctonia solani Kuhn, and Sclerotium rolfsii Sacc.) of cucumber plants under in vitro and greenhouse conditions. In vitro tests showed that all tested unautoclaved and unfiltrated composts water extracts (CWEs) had inhibitor effect against pathogenic fungi, compared to autoclaved and filtrated ones. Also, the inhibitor effects of 40 bacteria and 15 fungi isolated from composts were tested against the mycelial growth of cucumber root-rot pathogens. Twenty two bacteria and twelve fungal isolates had antagonistic effect against root-rot pathogens. The antagonistic fungal isolates were identified as 6 isolates belong to the genus Aspergillus spp., 5 isolates belong to the genus Penicillium spp. and one isolate belong to the genus Chaetomium spp. Under greenhouse conditions, the obtained results in pot experiment using artificial infested soil with cucumber root-rot pathogens showed that the compost amended soil reduced the percentage of disease incidence, pathogenic fungi population, and improved the cucumber vegetative parameters as shoot length, root length, fresh weight, and dry weight. These results suggested that composts are consequently considered as control measure against cucumber root-rot pathogens.

A Brief Look at Current Usda Work on Pests and Diseases

2021 ◽  
Vol 32 (4) ◽  
pp. 176-177
Keyword(s):  
Green Manure ◽  
Agricultural Research ◽  
Washington State ◽  
Water Filtration ◽  
White Mustard ◽  
Pests And Diseases ◽  
Natural Substances ◽  
University Scientists ◽  
Agricultural Research Service ◽  
Research Service

If a farmer has nematode problems or has too many weeds or fungal attacks a simple solution is to spread some mustard on them. Agricultural Research Service and university scientists are experimenting with mustards as an alternative to fighting crop pests chemically. The system biofumigates pests with stands of white mustard, brown mustard, and rapeseed.. Biofumigation refers to natural substances plants release while decomposing that make surrounding soils toxic to some weeds, nematodes, and fungi. The experiments, in Washington State, dovetail with increasing grower interest in mustard crops for pest control and as "green manure" meaning it can be disked into soil to improve tilth, organic matter, aeration, and water filtration. Despite such benefits, there is still much to learn about how mustards control pests and under what conditions they work best.

IMMU-53. STING-ING GLIOBLASTOMA WITH SPHERICAL NUCLEIC ACIDS

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi104-vi105
Author(s):  
Akanksha Mahajan ◽  
Lisa Hurley ◽  
Serena Tommasini-Ghelfi ◽  
Corey Dussold ◽  
Alexander Stegh ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Surface ◽  
Biological Properties ◽  
Innate Immune ◽  
Limiting Factors ◽  
Nucleic Acid Delivery ◽  
Sting Pathway ◽  
Spherical Nucleic Acids

Abstract The Stimulator of Interferon Genes (STING) pathway represents a major innate immune sensing mechanism for tumor-derived DNA. Modified cyclic dinucleotides (CDNs) that mimic the endogenous STING ligand cGAMP are currently being explored in patients with solid tumors that are amenable to intratumoral delivery. Inadequate bioavailability and insufficient lipophilicity are limiting factors for clinical CDN development, in particular when consideration is given to systemic administration approaches. We have shown that the formulation of oligonucleotides into Spherical Nucleic Acid (SNA) nanostructures, i.e.,the presentation of oligonucleotides at high density on the surface of nanoparticle cores, lead to biochemical and biological properties that are radically different from those of linear oligonucleotides. First-generation brain-penetrant siRNA-based SNAs (NCT03020017, recurrent GBM) have recently completed early clinical trials. Here, we report the development of a STING-agonistic immunotherapy by targeting cGAS, the sensor of cytosolic dsDNA upstream of STING, with SNAs presenting dsDNA at high surface density. The strategy of using SNAs exploits the ability of cGAS to raise STING responses by delivering dsDNA and inducing the catalytic production of endogenous CDNs. SNA nanostructures carrying a 45bp IFN-simulating dsDNA oligonucleotide, the most commonly used and widely characterized cGAS activator, potently activated the cGAS-STING pathway in vitro and in vivo. In a poorly immunogenic and highly aggressive syngeneic mouse glioma model, in which tumours were well-established, only one dose of intranasal treatment with STING-SNAs decelerated tumour growth, improved survival and importantly, was well-tolerated. Our use of SNAs addresses the challenges of nucleic acid delivery to intracranial tumor sites via intranasal route, exploits the binding of dsDNA molecules on the SNA surface to enhance the formation of a dimeric cGAS:DNA complex and establishes cGAS-agonistic SNAs as a novel class of immune-stimulatory modalities for triggering innate immune responses against tumor.

scholarly journals FORMULATION AND EVALUATION OF FLURBIPROFEN FAST DISINTEGRATING TABLETS USING NATURAL SUPERDISINTEGRANTS

2016 ◽  
Vol 9 (6) ◽  
pp. 247 ◽  
Author(s):  
Mohammed Sarfaraz ◽  
Surendra Kumar Sharma
Keyword(s):  
Drug Release ◽  
Angle Of Repose ◽  
Lepidium Sativum ◽  
Disintegration Time ◽  
Natural Sources ◽  
Weight Variation ◽  
Wetting Time ◽  
Tapped Density ◽  
Fast Disintegrating Tablets

ABSTRACTObjective: The main objective of this research was to formulate Fast disintegrating tablets of Flurbiprofen incorporating superdisintegrants, isolated from natural sources like Plantago ovata (PO) seeds, Lepidium sativum (LS) seeds and agar-agar.Methods: Superdisintegrants were isolated from their natural sources using reported methods. Swelling index and hydration capacity was determined for the natural superdisintegrants to know their disintegration capacity. The tablet formulations were designed using isolated natural superdisintegrants. The powder blends were evaluated for pre-compressional parameters like angle of repose, bulk density, tapped density, carr’s index, and hausner’s ratio. Fast disintegrating tablets were prepared by direct compression method. The compressed tablets were characterized for post compression parameters.Results: All formulations had hardness, friability, weight variation and drug content within the pharmacopoeial limits. The wetting time was 84 to 254 sec, in vitro disintegration time was between 59.2 to 221 sec, and in-vitro drug release was as low as 11.80% (LS1) to a maximum of 98.99% (PO4) after 4 min of study. Among all, optimized formulation was PO4, as it showed good wetting time (84 sec), fastest disintegration time (59.2 sec), dispersion time (135 sec) and drug release of 98.99.% within 4 min.Conclusion: Flurbiprofen FDT’s were successfully developed using isolated natural disintegrants. The natural disintegrants isolated showed promising results and can prove as effective alternative for synthetic disintegrants.

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