scholarly journals Protective Effect of Ethanolic Extract ofTabernaemontana divaricata(L.) R. Br. against DEN and Fe NTA Induced Liver Necrosis in Wistar Albino Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kannappan Poornima ◽  
Palanisamy Chella Perumal ◽  
Velliyur Kanniappan Gopalakrishnan
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatic Necrosis ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Protective Effects ◽  
Liver Necrosis ◽  
Standard Drug ◽  
Tabernaemontana Divaricata ◽  
Wistar Albino Rats

This study is an attempt to evaluate the hepatoprotective activity ofTabernaemontana divaricataagainst DEN and Fe NTA induced liver necrosis in rats. Ethanolic extract of the whole plant ofTabernaemontana divaricataat doses of 200 and 400 mg/kg body weight and 5-fluorouracil (standard drug) was orally administered to male Wistar Albino rats once daily for 24 weeks, simultaneously treated with the carcinogen DEN and Fe NTA. In simultaneously treated animals, the plant extract significantly decreased the levels of uric acid, bilirubin, AST, ALT, and ALP in serum and increased the levels of liver marker enzymes in liver. Treatment with the extracts resulted in a significant increase in the levels of antioxidants accompanied by a marked reduction in the levels of malondialdehyde when compared to DEN and Fe NTA treated group. When compared with 200 mg/kg bw rats, 400 mg/kg bw rats and 5-fluorouracil treated rats showed better results in all the parameters. The histopathological studies confirmed the protective effects of extract against DEN and Fe NTA induced liver necrosis. Thus, it could be concluded that the use ofTabernaemontana divaricataextract in the treatment of carcinogen induced hepatic necrosis.

Assessment of hepatoprotective activity of coded plant (222) leaf ethanolic extract against carbon tetrachloride-induced hepatotoxicity in Wistar rats – Part IV

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Krishnakumar N M
Keyword(s):  
Carbon Tetrachloride ◽  
Biochemical Parameters ◽  
Subcutaneous Administration ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Albino Rats ◽  
Hepatic Enzymes ◽  
Standard Drug

The present study was designed to assess the possible hepatoprotective activity of the leaf ethanolic extract of coded plant (Code No. 222**) against carbon tetrachloride (CCl4)-induced hepatic injury in Wistar albino rats. The animals were divided into different groups and treated with 222 leaf ethanolic extract at different concentrations for five days. Silymarin, the known hepatoprotective standard compound (100 mg/kg) was administered for five days. Hepatotoxicity was induced by the subcutaneous administration of a single dose of CCl4: Olive oil (2 mL/kg) on days 2 and 3. The administration of CCl4 resulted in marked increase in serum hepatic enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and serum bilirubin levels. CCl4 intoxication also resulted in a significant (P=0.05) increase in malondialdehyde (MDA), which is a common marker of lipid peroxidation. The other biochemical parameters such as cholesterol, triglycerides, creatinine, urea and uric acid levels were also increased significantly (P=0.05) compared to normal control group. Changes in serum hepatic enzymes, biochemical parameters and MDA levels induced by CCl4 were reversed by the leaf ethanolic extract of 222 (125 mg/kg) dose. The standard drug silymarin treated group also reversed CCl4-induced changes in biomarkers of liver function and MDA levels. Histopathological studies of the liver samples confirmed the hepatoprotective property of the coded drug 222. It was seen that histopathological damage induced by CCl4 were improved in rat liver, treated with 222 extract. The results of the present study suggested that coded plant (222) leaf ethanolic extract may be used as a hepatoprotective agent against toxic effects caused carbon tetrachloride in the liver.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA FRUIT AGAINST ETHANOL INDUCED HEPATOTOXICITY IN RATS

2017 ◽  
Vol 10 (11) ◽  
pp. 55
Author(s):  
Balakrishna Vuyyala ◽  
Lakshmi Thakkalapally
Keyword(s):  
Ethanolic Extract ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Standard Group ◽  
Terminalia Chebula ◽  
Fruit Extract ◽  
Treatment Protocols ◽  
Standard Drug ◽  
Antioxidant Parameters

  Objective: The purpose of this study was to evaluate the effect of Terminalia chebula fruit extract on liver antioxidant enzymes in ethanol-induced hepatotoxicity in rats.Method: Rats were divided into six different groups each having six. Group 1 served as a control, Group 2 received 40% ethanol (2 ml/100 g, oral), in sterile water, Groups 4, 5, and 6 served as extract treatment groups and received 50, 100, and 200 mg/kg, orally, ethanolic fruit extract of T. chebula (TCE) and Group 3 served as standard group and received silymarin 25 mg/kg orally. All the treatment protocols followed 21 days, and after which rats were sacrificed, the liver was taken for antioxidant and histological studies, respectively.Results: The ethanol-treated group rats (G2) showed variable decrease in antioxidant parameter (catalase, glutathione, and glutathione reductase) levels. Administration of ethanolic TCE significantly prevented ethanol-induced elevation in the levels of malondialdehyde lipid peroxidation and decreased antioxidant parameters in experimental groups of rats. The effect of extract was compared with a standard drug, silymarin. The changes in antioxidant parameters were supported by histological profile.Conclusion: It is concluded that the ethanolic fruit TCE protects against ethanol-induced oxidative liver injury in rats.

scholarly journals Ocimum Sanctum Ameliorates Valproic Acid – Induced Hepatotoxicity

2020 ◽  
Author(s):  
Muhammad Omer Iqbal ◽  
Qurratulain Rahim ◽  
Hania Mehboob Khan ◽  
Imran Akhter ◽  
Faiza Naeem ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Normal Saline ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Ocimum Sanctum ◽  
Albino Rats ◽  
Reduced Pressure ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Different Doses

Abstract OBJECTIVE: Evaluation of hepatoprotective potentials of Ocimum sanctum against Valproic-acid-induced-hepatotoxicity in Wistar-albino-rats. METHOD: 70% of ethanolic extract of Ocimum sanctum was prepared under reduced pressure of rotary evaporator. Wistar albino rats were used as the experimental model and rats were divided into four groups (six animals each). The normal group received normal saline and group 2, 3 and 4 was injected valproic acid (500mg/kg) for four consecutive days respectively. Group 1 and 2 received normal saline throughout the period of study about 21 days while group 3 and 4 received different doses of extract of OS i.e. 200mg/kg and 300mg/kg. Through retro-orbital blood samples were collected on alternative days such as 0,7,21. By using one-way ANOVA, data was analyzed. Hepatotoxicity induced by valproic acid at the dosage of (500mg/kg) resulted in significant elevation in weight of animals and serum hepatic enzymes level of ALAT, ASAT, ALP and increase in the serum bilirubin. RESULTS: OS at different doses (200mg/kg and 300mg/kg) considered statistically significant (p ≤ 0.05) against all parameters. OS cause a significant reduction in weight of animals and serum enzymes biomarkers i.e. (ALAT, ASAT and ALP) including bilirubin content. OS may prove its hepatoprotective activity by increase a significant level of protein albumin. CONCLUSION: antioxidant activity of OS and secondary metabolites such as flavonoids depicts hepatoprotective nature against valproic-acid-induced-hepatotoxicity.

scholarly journals Protective effects of vitamin E on central nervous system in streptozotocin-induced diabetic rats

2009 ◽  
Vol 32 (5) ◽  
pp. 314 ◽  
Author(s):  
Sibel Canbaz Kabay ◽  
Hilmi Ozden ◽  
Gul Guven ◽  
M Cengiz Ustuner ◽  
Irfan Degirmenci ◽  
...  
Keyword(s):  
Vitamin E ◽  
Treated Group ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Albino Rats ◽  
Protective Effects ◽  
Neuroprotective Effects ◽  
Wistar Albino Rats ◽  
Group B ◽  
Group D

Objective: To evaluate the histopathological and antioxidant effects of vitamin E (VE) treatment on brain tissue in streptozotocin (STZ)-induced diabetic rats. Methods: Thirty two male Wistar albino rats were used. The study comprised four groups of 8 rats: Group A - untreated group, group B - diabetic group, group C - VE and group D - diabetic plus VE. In the diabetic groups, diabetes was induced by a single intraperitoneal injection of 65 mg/kg STZ. Vitamin E was given 50 mg/kg/day i.p. for three weeks. Concentrations of glucose, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were detected in the haemolysate. Results: Glucose concentrations were increased in the blood of the STZ-treated rats compared with those in the diabetic groups (group B and D). The MDA concentrations in the brain from diabetic rats increased, whereas the GPx, SOD, CAT concentrations decreased. Treatment with VE returned concentrations of MDA, GPx, SOD and CAT toward control values. The MDA concentration in the diabetic group (20.65±2.24 nmol/mg Hb) was decreased compared with the VE treated group (15.54±1.32 nmol/mg Hb). There were no pathological differences between untreated and VE treated rats’ brains. Neuronal ischemic damages were determined in STZ-induced diabetic rats. Ischemic neuronal alterations in group B (diabetic) had more damage than group D (diabetic + VE). Conclusion: The study revealed neuroprotective effects of VE on ischemic damage in diabetic central neuronal cells, caused by diabetic oxidative stress.

scholarly journals EVALUATION OF HEPATOPROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF ROOT OF PUNICA GRANATUM IN RATS

2017 ◽  
Vol 10 (7) ◽  
pp. 159
Author(s):  
Bushra Hasan Khan ◽  
Farida Ahmad ◽  
Jameel Ahmad ◽  
Syed Mobashir Yunus
Keyword(s):  
Liver Injury ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Total Serum ◽  
Physical Parameters ◽  
Standard Drug

Objective: To evaluate the hepatoprotective effect of ethanolic extract of the root (REE) of Punica granatum.Methods: This study was conducted on adult albino Wistar rats of either sex weighing 150-200 g. Animals were divided into five groups (n=5). Liver injury was produced by carbon tetrachloride (CCl4) 1 ml/kg dissolved in olive oil (1:1) given intraperitoneally on day 1 and day 4 of the study duration of 14 days. Silymarin (50 mg/kg/d) orally was used as standard drug. Test groups received an REE of P. granatum (REE) at doses of 200 and 400 mg/kg/day orally along with CCl4. On the 15th day, all animals were sacrificed, and blood was collected. Liver was sent for histopathological examination. The hepatoprotective effect of REE was evaluated by assessment of physical parameters, histopathological examination and biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum bilirubin.Results: The administration of REE of P. granatum at doses of 200 and 400 mg/kg/day orally, exhibited a highly significant decrease in the rise of mean serum AST, ALT, ALP, and total bilirubin as compared to CCl4 treated group (p<0.001). Histopathological examination of the liver also suggested hepatoprotective effect of REE of P. granatum by restoration of hepatic architecture toward normal. Decrease in the extent of centrilobular necrosis was observed in REE (200 and 400 mg/kg/day) treated rats when compared to CCl4 treated group.Conclusion: This study demonstrated hepatoprotective activity of REE of P. granatum against CCl4 induced liver injury in rats.

GASTROPROTECTIVE AND ULCER HEALING ACTIVITY OF ETHANOLIC EXTRACT OF ADIANTUM LUNULATUM

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (03) ◽  
pp. 68-73
Author(s):  
M. N Deshpande ◽  
N Balekar ◽  
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
Monsoon Season ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Bleeding Disorders ◽  
Standard Drug ◽  
Pylorus Ligation ◽  
Healing Property ◽  
Wistar Albino Rats

Adiantum lunulatum is a fern found in monsoon season in Western Ghats, which is being used traditionally for many ailments such as bronchitis, cough, erysipelas and bleeding disorders. Aim of the present study was to investigate traditional claim of treating bleeding disorder and ulcer healing property of this plant. In the current study, ethanolic extract of adiantum lunulatum (500mg/kg) was evaluated for its efficacy in treating gastric ulcer in wistar albino rats. Ulcers were induced by different ulcer inducing models such as pylorus ligation induced ulcer, ethanol induced ulcer, cold restrain induced ulcer and aspirin induced ulcer. Omeprazole was used as a standard drug at a dose of 20mg/kg. Adiantum lunulatum showed a significant reduction in gastric ulcer, which was significantly comparable to standard drug omeprazole.

scholarly journals EVALUATION OF ANTIUROLITHIATIC PROPERTY OF ETHANOLIC EXTRACT OF FENNEL SEEDS IN MALE WISTAR ALBINO RATS

2017 ◽  
Vol 10 (8) ◽  
pp. 313
Author(s):  
Basvaraj Poojar ◽  
Balaji Ommurugan ◽  
Shalini Adiga ◽  
Huban Thomas
Keyword(s):  
Ethylene Glycol ◽  
Stone Formation ◽  
Ethanolic Extract ◽  
Control Group ◽  
Albino Rats ◽  
Foeniculum Vulgare ◽  
Standard Drug ◽  
Wistar Albino Rats ◽  
Treatment Of Urolithiasis ◽  
Different Doses

Objective: Few studies have explored the diuretic property of fennel (Foeniculum vulgare). Hence, the aim of this study was to evaluate the antiurolithiatic property of ethanolic extract of fennel seeds in male Wistar albino rats.Methods: Prophylactic and curative urolithiasis models were used with 5 groups of 6 rats in each model. Ethanolic extract of fennel seeds in three doses 100, 200, and 300 mg/kg was used. Cystone 750 mg/kg was used as a standard drug. All drugs were administered orally. Zinc discs were surgically implanted in the bladder in all rats. After recovery, rats in the prophylactic model received three different doses of ethanolic extract of fennel seeds along with 1% ethylene glycol for 2 weeks whereas the rats in the other model received 1% ethylene glycol for 2 weeks followed by an ethanolic extract of fennel seeds in three doses for the next 2 weeks. Both models had a control group receiving 1% ethylene glycol. At the end of study period, rats were sacrificed and vesical calculi collected, weighed, and statistically evaluated using one-way ANOVA.Results: In both the models, all three doses of an extract of fennel seeds were effective in reducing stone formation as compared to control group with p<0.05. In both the models, all three test doses were comparable with cystone, but 300 mg/kg extract in prophylactic showed significance (p <0.05) when compared to standard.Conclusion: Fennel seeds can be used prophylactically as well as curatively in the treatment of urolithiasis. However, further studies and clinical trials are warranted to explore this property.

scholarly journals Effect of Adhatoda zeylanica Ethanolic Extract on Attenuated Kidney in Streptozotocin-Induced Diabetic Rats

2021 ◽  
Author(s):  
Prima Swetha D’souza ◽  
Rajendra Holla ◽  
Gangadhara Swamy
Keyword(s):  
Serum Creatinine ◽  
Ethanolic Extract ◽  
Tissue Homogenate ◽  
Albino Rats ◽  
Standard Drug ◽  
Once Daily ◽  
Wistar Albino Rats ◽  
Group B ◽  
Histopathological Studies ◽  
Group D

Abstract Objective The present study was aimed to evaluate the effect of ethanolic extract of Adhatoda zeylanica (EAZ) leaves on streptozotocin (STZ)-induced diabetes mellitus (DM) and its renal complications in male Wistar albino rats. Materials and Methods Adult male Wistar albino rats were randomly selected from a colony, divided into four groups, namely, A, B, C, and D, with each having six rats (n = 6) and each weighing between 200 and 250 g. Group A served as control and received only water per oral (p.o.). Group B, C, and D animals received a single dose of STZ at 45 mg/kg body weight (kbw) intraperitoneal administration (i.p.) on day 1 and observed for fasting blood glucose (FBG) to induce DM for next 72 hours. After the DM was induced, group B served as DM control, group C received the standard drug glibenclamide (GL) at 5 mg/kbw p.o. once daily, and group D received EAZ of 500 mg/kbw p.o. once daily for 35 days. After the observation period, the animals were euthanized, serum creatinine and blood urea, antioxidants in the kidney tissue homogenate, and histopathological studies were assessed to know the ameliorative effect of the test drugs. Results Renal parameters, such as serum creatinine, blood urea, antioxidants activities, in group D were nearer to the control when compared with groups B and C. Histopathological studies revealed that there was minimal renal damage in group D when compared with groups B and C. Conclusion Administration of ethanolic EAZ showed significant ameliorative effects on the FBG, biochemical, oxidative, and histopathological parameters on kidney tissues treated with STZ to induce DM.

scholarly journals HEPATOPROTECTIVE ACIVITY OF THE ETHANOLIC EXTRACT OF DOG FRUIT RIND (Pithecellobium lobatum Benth.)

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
Ruqiah Ganda Putri Panjaitan ◽  
Ely Savitri ◽  
Titin Titin
Keyword(s):  
Body Weight ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Alanine Transaminase ◽  
Albino Rats ◽  
Aspartate Transaminase ◽  
Wistar Albino Rats ◽  
Fruit Rind

The present study was conducted to investigate the hepatoprotective activity of ethanolic extract of dog fruit rind (Pithecellobium lobatum benth.) against carbon tetrachloride (CCl4) induced hepatotoxicity in rats. Male Wistar albino rats were randomly divided into four groups and administered orally with 50 mg/200 g body weight of dog fruit rind extract (K1), 100 mg/200 g body weight (K2) of dog fruit rind extract, 5 mg/200 g body weight of silymarin (K3/positive control), and 0.4 mL/200 g body weight of distilled water (K4/negative control), for seven days The levels of alanine transaminase (ALT) of each K1; K2; K3; and K4 were 143.40±83.75 U/L, 94.80±93.77 U/L, 130.20±58.54 U/L and 147.25±107.97 U/L, respectively, while the aspartate transaminase (AST) levels were 304.20±128.67 U/L; 213.20±88.93 U/L; 333.00±128.31 U/L; and 239.25 ± 94.90 U/L, respectively (P>0.05). Group K2 showed better histological pattern than other groups with 60% of mild and 40% of moderate liver damage. Our findings revealed the hepatoprotective activity of the ethanolic extract of dog fruit rind.

scholarly journals study Study of the Hepatoprotective Activity of Polyherbal Formulation on Alcohol Induced Hepatotoxicity

2019 ◽  
Vol 9 (6-s) ◽  
pp. 16-20
Author(s):  
ZALAK CHIRAG SHAH ◽  
Archana Paranjape ◽  
Hardik Soni ◽  
Snigdha Das Mandal ◽  
Janki Patel
Keyword(s):  
Liver Weight ◽  
Sleep Time ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Group Iii ◽  
Polyherbal Formulation ◽  
Group I ◽  
Group Ii ◽  
Wistar Albino Rats

Many traditional systems of medicines employ herbal drugs for the hepatoprotection. Aim of the study was designed to evaluate the hepatoprotective potential of polyherbal formulation against alcohol induced hepatotoxicity in wistar albino rats. Group I animals were treated with 1% CMC for 18 days. Group II, III and IV animals were treated with 1% CMC, polyherbal formulation 180mg/kg/day and silymarin 100mg/kg/day respectively for 18 days and then orally administration with ethanol 3.76 g/kg/day simultaneously for 18 days. After 24 hours of last dosing, the blood was obtained through retro-orbital plexus under light anaesthesia and the animals were sacrificed.  Hepatoprotective potential was assessed by various biochemical parameters such as AST, ALT, ALP, LDH, bilirubin, cholesterol, TG and thiopentone sodium induced sleep time. Group III rats showed significant (p<0.01) decrease in AST, ALT, ALP, LDH, bilirubin, cholesterol, TG, liver weight(wt.) and relative liver wt. levels while significant (p<0.01) increase in TP levels as compared to group II rats. Hepatoprotective potential of polyherbal formulation 180mg/kg/day was comparable to that of standard drug silymarin 100mg/kg/day. Results of the study were well supported by histopathological observations. This study confirms that polyherbal formulation possesses hepatoprotective potential comparable to that of standard drug silymarin as it exhibited comparable protective potential against PCM induced hepatotoxicity in albino rats. Keywords: Polyherbal formulation, Hepatoprotective potential, Alcohol, Hepatotoxicity, Silymarin

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