scholarly journals Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

2014 ◽  
Vol 2014 ◽  
pp. 1-20 ◽  
Author(s):  
Priyamvada Jain ◽  
Babina Chakma ◽  
Sanjukta Patra ◽  
Pranab Goswami
Keyword(s):  
Lactate Dehydrogenase ◽  
Malaria Control ◽  
Rapid Detection ◽  
Diagnostic Techniques ◽  
Current Status ◽  
Drug Resistant ◽  
Detection Techniques ◽  
Reliable Detection ◽  
Proper Diagnosis ◽  
Potential Biomarkers

Malaria has been responsible for the highest mortality in most malaria endemic countries. Even after decades of malaria control campaigns, it still persists as a disease of high mortality due to improper diagnosis and rapidly evolving drug resistant malarial parasites. For efficient and economical malaria management, WHO recommends that all malaria suspected patients should receive proper diagnosis before administering drugs. It is thus imperative to develop fast, economical, and accurate techniques for diagnosis of malaria. In this regard an in-depth knowledge on malaria biomarkers is important to identify an appropriate biorecognition element and utilize it prudently to develop a reliable detection technique for diagnosis of the disease. Among the various biomarkers, plasmodial lactate dehydrogenase and histidine-rich protein II (HRP II) have received increasing attention for developing rapid and reliable detection techniques for malaria. The widely used rapid detection tests (RDTs) for malaria succumb to many drawbacks which promotes exploration of more efficient economical detection techniques. This paper provides an overview on the current status of malaria biomarkers, along with their potential utilization for developing different malaria diagnostic techniques and advanced biosensors.

scholarly journals Advanced and futuristic approaches for breast cancer diagnosis

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Jayanti Mishra ◽  
Bhumika Kumar ◽  
Monika Targhotra ◽  
P. K. Sahoo
Keyword(s):  
Breast Cancer ◽  
Early Diagnosis ◽  
Cancer Diagnosis ◽  
Breast Cancer Diagnosis ◽  
Diagnostic Techniques ◽  
Main Body ◽  
Detection Techniques ◽  
Initial Stage ◽  
Common Causes ◽  
Delay In Treatment

Abstract Background Breast cancer is the most frequent cancer and one of the most common causes of death in women, impacting almost 2 million women each year. Tenacity or perseverance of breast cancer in women is very high these days with an extensive increasing rate of 3 to 5% every year. Along with hurdles faced during treatment of breast tumor, one of the crucial causes of delay in treatment is invasive and poor diagnostic techniques for breast cancer hence the early diagnosis of breast tumors will help us to improve its management and treatment in the initial stage. Main body Present review aims to explore diagnostic techniques for breast cancer that are currently being used, recent advancements that aids in prior detection and evaluation and are extensively focused on techniques that are going to be future of breast cancer detection with better efficiency and lesser pain to patients so that it helps to a physician to prevent delay in treatment of cancer. Here, we have discussed mammography and its advanced forms that are the need of current era, techniques involving radiation such as radionuclide methods, the potential of nanotechnology by using nanoparticle in breast cancer, and how the new inventions such as breath biopsy, and X-ray diffraction of hair can simply use as a prominent method in breast cancer early and easy detection tool. Conclusion It is observed significantly that advancement in detection techniques is helping in early diagnosis of breast cancer; however, we have to also focus on techniques that will improve the future of cancer diagnosis in like optical imaging and HER2 testing.

scholarly journals Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

2021 ◽  
Vol 13 (2) ◽  
pp. 518-539
Author(s):  
Peuli Nath ◽  
Md Alamgir Kabir ◽  
Somaiyeh Khoubafarin Doust ◽  
Aniruddha Ray
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Point Of Care ◽  
Diagnostic Techniques ◽  
Nucleic Acid Amplification ◽  
Physical Contact ◽  
Detection Sensitivity ◽  
Detection Techniques ◽  
Advantages And Disadvantages ◽  
Simplex Virus

Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

scholarly journals Optical Detection and Eradication of Dysplastic Barrett's Esophagus

2003 ◽  
Vol 2 (4) ◽  
pp. 289-302 ◽  
Author(s):  
Louis-Michel Wong Kee Song ◽  
Kenneth K. Wang
Keyword(s):  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Sampling Error ◽  
Imaging Techniques ◽  
Standard Of Care ◽  
Diagnostic Techniques ◽  
Endoscopic Surveillance ◽  
Current Status ◽  
Delay In Diagnosis ◽  
Multiple Biopsies

Dysplastic Barrett's esophagus is a condition that offers multiple diagnostic and therapeutic challenges. The diagnosis of dysplasia within Barrett's esophagus currently relies on periodic endoscopic surveillance with multiple biopsies, a methodology limited by random sampling error, inconsistent histopathologic interpretation and delay in diagnosis. Optical spectroscopic and imaging techniques have the potential to identify dysplastic or early neoplastic lesions in real-time. These diagnostic modalities are needed to enhance the endoscopic surveillance of Barrett's esophagus in the future as well as help to define lesions for endoscopic therapy. Esophagectomy has been the standard of care for Barrett's esophagus with high-grade dysplasia although it is a procedure associated with significant morbidity and mortality. Minimally invasive endoscopic ablative therapies are attractive and less morbid alternatives to esophagectomy, with promising results obtained from the use of light-activated drugs (i.e., photodynamic therapy). The combination of novel optical diagnostic techniques and therapies will provide the endoscopist with much needed tools that can considerably enhance the management of patients with Barrett's esophagus. This article reviews the current status and future prospects of optical-based modalities for diagnosis and therapy of dysplastic Barrett's esophagus.

Study on Techniques for Rapidly Testing the Composition of Concrete Mixture

2013 ◽  
Vol 838-841 ◽  
pp. 30-35
Author(s):  
Xiao Bin Sun ◽  
Tian Ling Du ◽  
Bo Tian
Keyword(s):  
Quality Control ◽  
Laboratory Test ◽  
Rapid Detection ◽  
Measurement Accuracy ◽  
Fresh Concrete ◽  
Concrete Mixture ◽  
Quality Problem ◽  
Detection Techniques ◽  
Concrete Quality ◽  
Practical Engineering

With regards to the quality problem of concrete that cant be found out earlier in the quality control, this paper discussed the principle to rapidly detect composition of concrete mixture and analyzed the measurement accuracy of water checker for fresh concrete through laboratory test. Experiments showed that the error in unit cement and unit water measured by instruments is relatively small and able to meet the accuracy requirements. And, the application of rapid detection techniques to the concrete quality control in practical engineering has achieved good results.

scholarly journals Rapid Detection and Evaluation of Clinical Characteristics of Emerging Multiple-Drug-Resistant Gram-Negative Rods Carrying the Metallo-β-Lactamase GeneblaIMP

1998 ◽  
Vol 42 (8) ◽  
pp. 2006-2011 ◽  
Author(s):  
Yoichi Hirakata ◽  
Koichi Izumikawa ◽  
Toshiyuki Yamaguchi ◽  
Hiromu Takemura ◽  
Hironori Tanaka ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Rapid Detection ◽  
Clinical Characteristics ◽  
The Other ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Gram Negative ◽  
Multiple Drug Resistant ◽  
Tract Infections

Gram-negative rods (GNR) carrying the transferable carbapenem resistance gene blaIMP, includingPseudomonas aeruginosa and Serratia marcescens, have been isolated from more than 20 hospitals in Japan. Although the emergence of such multiple-drug-resistant bacteria is of utmost clinical concern, little information in regard to the distribution ofblaIMP-positive GNR in hospitals and the clinical characteristics of infected patients is available. To address this, a system for the rapid detection of theblaIMP gene with a simple DNA preparation and by enzymatic detection of PCR products was developed. A total of 933 ceftazidime-resistant strains of GNR isolated between 1991 and 1996 at Nagasaki University Hospital, Nagasaki, Japan, were screened for theblaIMP gene; 80 isolates were positive, including 53 P. aeruginosa isolates, 13 other glucose-nonfermenting bacteria, 13 S. marcescens isolates, and 1 Citrobacter freundii isolate. Most of the patients from whom blaIMP-positive organisms were isolated had malignant diseases (53.8%). The organisms caused urinary tract infections, pneumonia, or other infections in 46.3% of the patients, while they were just colonizing the other patients evaluated. It was possible that blaIMP-positive P. aeruginosa strains contributed to the death of four patients, while the other infections caused by GNR carryingblaIMP were not lethal. DNA fingerprinting analysis by pulsed-field gel electrophoresis suggested the cross transmission of strains within the hospital. The isolates were ceftazidime resistant and were frequently resistant to other antibiotics. Although no particular means of pathogenesis ofblaIMP-positive GNR is evident at present, the rapid detection of such strains is necessary to help with infection control practices for the prevention of their dissemination and the transmission of the resistance gene to other pathogenic bacteria.

scholarly journals Current status of major foodborne and waterborne viral infections and their prevention strategies

2021 ◽  
Vol 7 (3) ◽  
pp. 227-241
Author(s):  
Ahmet Gökhan Coşkun ◽  
Ayşegül Demircioğlu ◽  
Seran Temelli ◽  
Ayşegül Eyigör
Keyword(s):  
Viral Infections ◽  
Hemorrhagic Fever ◽  
Diagnostic Techniques ◽  
Control Measures ◽  
Current Status ◽  
Waterborne Diseases ◽  
Antiviral Compounds ◽  
Current Prevalence ◽  
Haccp System ◽  
Waterborne Viruses

Advances in diagnostic techniques and their widespread use for infectious agents revealed the considerably high current prevalence of viral agents in foodborne and waterborne diseases. Foodborne and waterborne viruses are indicated to cause not only gastroenteritis and hepatitis but also neurological disorders, respiratory tract diseases, myocarditis, glomerulonephritis and hemorrhagic fever, with a particularly high mortality rate in infants/children and in individuals with immune deficiency. Additionally, due to their resistance to environmental conditions and food processes compared to other microorganisms, elimination of these viruses by heat and high pressure applications, natural antiviral compounds, UV applications and conventional cleaning-disinfection remains difficult even inadequate. In protection from viral infections, vaccine applications together with GMP, GHP and HACCP system approaches in production seem to be the most effective approaches to ensure the minimization of viruses in food environment and in public. In this review article, up-to-date information is presented on the general characteristics and the diseases caused by enterotropic viruses; NoV, AstV, RoV, AdV and hepatotropic viruses; HAV and HEV, with a particularly high worldwide prevalence, as well as their epidemiology, prevention and their control measures.

scholarly journals Prevalence of Staphylococcus aureus and Methicillin-resistant S. aureus in Adult Hospitalized Patients in “Mother Theresa” Hospital Center in Albania

2020 ◽  
Vol 8 (A) ◽  
pp. 297-302
Author(s):  
Blerta Kika ◽  
Erjona Abazaj ◽  
Oltiana Petri ◽  
Andi Koraqi
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Latex Agglutination ◽  
Hospitalized Patients ◽  
Hospital Environment ◽  
Drug Resistant ◽  
Methicillin Resistant ◽  
Clinical Specimens ◽  
Microbial Identification ◽  
Hospital Center

AIM: The aim of this study was to evaluate the prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in clinical specimens hospitalized to “Mother Theresa” Hospital Center for 2 years. METHODS: We isolated and identified S. aureus on 356 clinical specimens using standard tests. Furthermore, for further accurate microbial identification, we have to use the VITEK® 2 system. The samples were tested to detect the presence of MRSA by a slide latex agglutination kit for the rapid detection of PBP2. RESULTS: The overall prevalence of S. aureus in patients was 34.2%. The prevalence of MRSA was 20.5% of cases. Of the MRSA isolates identified in this study, 28% were susceptible to antibiotics, 24% demonstrated intermediate resistance, and 48% were multi-drug resistant with resistance to nineteen antibiotics involved in the examination. In addition, seven of the 25 MRSA cases showed 100% resistance to norfloxacin, imipenem, meropenem, levofloxacin, etc. CONCLUSIONS: The rate of S. aureus in hospitalized patients on this study was 34.2% and the MRSA 20.5%. These results indicated that this type of infection is a significant concern for health services and patients included. A screening of all hospitalized cases can lead to reduce the incidence of this infection in the hospital environment.

scholarly journals The Current Status of Drug-resistant Aspergillus

2016 ◽  
Vol 57 (3) ◽  
pp. J103-J112 ◽  
Author(s):  
Masato Tashiro ◽  
Koichi Izumikawa
Keyword(s):  
Current Status ◽  
Drug Resistant
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