scholarly journals Evaluation of Biological Activities of Chemically Synthesized Silver Nanoparticles

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Ashraf A. Mostafa ◽  
Shaban R. M. Sayed ◽  
Essam N. Solkamy ◽  
Mujeeb Khan ◽  
Mohammed Rafi Shaik ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biological Activities ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
X Ray ◽  
Transmission Electron ◽  
Antiseptic Agent ◽  
Synthesized Materials ◽  
Strong Antimicrobial Activity

Silver nanoparticles were synthesized by the earlier reported methods. The synthesized nanoparticles were characterized using ultraviolet-visible spectrophotometry (UV/Vis), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), and X-ray powder diffraction (XRD). The synthesized materials were also evaluated for their antibacterial activity against Gram positive and Gram negative bacterial strains. TEM micrograph showed the spherical morphology of AgNPs with size range of 40–60 nm.The synthesized nanoparticles showed a strong antimicrobial activity and their effect depends upon bacterial strain as AgNPs exhibited greater inhibition zone forPseudomonas aeruginosa(19.1 mm) followed byStaphylococcus aureus(14.8 mm) andS. pyogenes(13.6 mm) while the least activity was observed forSalmonella typhi(12.5 mm) at concentration of 5 µg/disc. The minimum inhibitory concentration (MIC) of AgNPs againstS. aureuswas 2.5 µg/disc and less than 2.5 µg/disc forP. aeruginosa. These results suggested that AgNPs can be used as an effective antiseptic agent for infectious control in medical field.

scholarly journals Biophysical and Characterizations of Silver Nanoparticles used as Salmonella typhi Detector

2014 ◽  
Vol 2 (4) ◽  
pp. 510-515
Author(s):  
Hala Moustafa Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Combined Action ◽  
Hog Plum ◽  
Transmission Electronmicroscopy

The present study mainly focuses of combined action of Nepali hog plum as well as citrate synthesized silver nanoparticles (AgNPs) and Amikacin, as an antibiotic. The synergistic actions of citrate stabilized silver nanoparticles (AgNPs with chem) were compared with that of Nepali hog plum Choerospondia saxillaris (Lapsi) synthesized silver nanoparticles (AgNPs with plant), together with action of antibiotic onselected bacterial strains of Salmonella typhi. The synthesized AgNPs were characterized through UV-Vis spectroscopy, Transmission electronmicroscopy and X-ray diffraction technique. The size of the synthesized silver nanoparticles was measured by Transmission Electron Microscope (TEM) and X-ray diffraction (XRD).DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11127 Int J Appl Sci Biotechnol, Vol. 2(4): 510-515 

scholarly journals Eco Friendly Approach for Synthesis, Characterization and Biological Activities of Milk Protein Stabilized Silver Nanoparticles

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1418 ◽  
Author(s):  
Sadanand Pandey ◽  
Corli De Klerk ◽  
Joonwoo Kim ◽  
Misook Kang ◽  
Elvis Fosso-Kankeu
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Milk Protein ◽  
Biological Activities ◽  
Salmonella Typhi ◽  
Aspergillus Ochraceus ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Ultrahigh Temperature ◽  
And Staphylococcus Aureus

Today, the overall occurrence of re-emerging and rising illnesses has been a serious load on economies as well as public health. Here, we describe a simple, nontoxic and eco-friendly method for the synthesis of milk protein (MP)-stabilized silver nanoparticles (MP-s-AgNPs) using ultrahigh-temperature full cream milk. Highly stable AgNPs were prepared with a fair control over their size, without using any reducing or stabilizing agent, and their formation was attributed to the presence of the MP casein. Ag+ ion reduction was possibly caused by the MPs. The synthesized MP-s-AgNPs were characterized in detail by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy and dynamic light scattering. MP-s-AgNPs showed inhibitory activity against both Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative microorganisms (Salmonella typhi and Escherichia coli). Moreover, MP-s-AgNPs were found to be more toxic to bacteria than to fungi (Aspergillus fumigatus, Aspergillus ochraceus and Penicillium chrysogenum).

scholarly journals Synthesis and Catalytic and Biological Activities of Silver and Copper Nanoparticles Using Cassia occidentalis

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Manjul Gondwal ◽  
Geeta Joshi nee Pant
Keyword(s):  
Electron Microscopy ◽  
Metal Nanoparticles ◽  
Copper Nanoparticles ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Salmonella Typhi ◽  
Cassia Occidentalis ◽  
X Ray ◽  
Transmission Electron

An ecofriendly and green method for the synthesis of silver and copper nanoparticles has developed using aqueous leaves extract of Cassia occidentalis. The formation of AgNPs and CuNPs was monitored by measuring the UV-Vis spectra. The morphology and crystalline phase of the metal nanoparticles were determined using transmission electron microscopy (TEM) and scanning electron microscopy (SEM) with X-ray energy dispersive spectrophotometer (EDX) and X-ray diffraction (XRD). The synthesized metal nanoparticles were generally found to be spherical and oval in shape. The AgNPs and CuNPs showed highly potent antibacterial activity against Escherichia coli and Salmonella typhi bacteria, respectively. The CuNPs showed higher radical scavenging activity than AgNPs. The AgNPs showed lower haemolysis (1.7%) exhibiting lesser toxicity as compared to CuNPs. The CuNPs have better catalytic ability for the reduction of 4-nitrophenol and 2-nitrophenol as compared to AgNPs.

scholarly journals PRELIMINARY STUDIES ON ISOLATION AND SCREENING OF ANTIBIOTIC PRODUCING SYMBIOTIC BACTERIA FROM STARFISH (Protoreaster nodosus) COLLECTED FROM COASTAL AREA TAKALAR REGENCY, INDONESIA

2021 ◽  
Vol 9 (2) ◽  
pp. 183-188
Author(s):  
Muhammad Danial Fajri ◽  
◽  
Subehan Lallo ◽  
Sartini Sartini ◽  
◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Coastal Area ◽  
Biological Activities ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Symbiotic Bacteria ◽  
Bacterial Strains ◽  
E Coli ◽  
Sterile Seawater

Marine organisms are well known for the availability of bioactive compounds which have various biological activities including antibacterial activity. Likewise, their symbiotic bacteria can also produce compounds that have similar activities. The purpose of this study was to isolate and screen the symbiotic bacteria from starfish (Protoreaster nodosus) collected from coastal area Takalar Regency, South Sulawesi, Indonesia. Isolation was carried out by the pour plate method using nutrient agar medium dissolved in sterile seawater. The isolated symbiotic bacteria were purified by using the quadrant method. The pure isolate was culture through submerged fermentation using nutrient broth media enriched with 1% yeast extract and sterile seawater for 7 days. The selected symbiont bacterial isolates were tested for their antibacterial activity against gram-positive and gram-negative bacteria using disc diffusion assays. The results of fermentation were separated from the biomasses and tested for antibacterial activity against Staphylococcus aureus (S. aureus, ATCC 25923), Bacillus subtilis (B. subtilis, ATCC 6633), Salmonella typhi (S. typhi, NCTC 786), and Escherichia coli (E. coli, ATCC 25923). The results of study revealed that four symbiotic bacteria (SB 1T, SB 2T, SB 3T, and SB 4T) were successfully isolated. All the SB isolates have good antibacterial activity against all tested bacterial strains with an average diameter of inhibition zone larger than 11 mm. Among all isolates, isolate SB 4T showed a remarkable size of zones growth inhibition (> 15 mm) against all tested bacterial strains. Thus, the symbiotic bacteria isolated from P. nodosus in this study have a promising broad-spectrum antibacterial activity.

scholarly journals Biosynthesis of Silver Nanoparticles by Aspergillus terreus: Characterization, Optimization, and Biological Activities

2021 ◽  
Vol 9 ◽  
Author(s):  
Walid A. Lotfy ◽  
Basma M. Alkersh ◽  
Soraya A. Sabry ◽  
Hanan A. Ghozlan
Keyword(s):  
Silver Nanoparticles ◽  
Aspergillus Terreus ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antagonistic Activity ◽  
Metallic Silver ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron

In this study, mycelial filtrate of Aspergillus terreus BA6 was used to reduce AgNO3 to form silver nanoparticles (AgNPs). The effect of seven independent variables on the diameter of AgNPs was studied by applying design of experiments (DOE). At optimal conditions, the diameter of AgNPs was reduced by approximately 26.7% compared to the basal culture condition and AgNO3 concentration was found to be the most significant factor affecting the diameter of AgNPs. A. terreus nano-Ag was characterized using UV-visible spectroscopy, transmission electron microscopy, energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Zeta potential. The maximum UV absorption was obtained at 420 nm and the microscopic results showed particles with narrow size distribution ranging from 7 to 23 nm. XRD pattern of AgNPs revealed four diffraction peaks of metallic silver and the EDX spectrum showed a strong signal attributed to Ag nano-crystals. AgNPs mycofabricated by A. terreus showed potent minimum inhibitory concentration (MIC) and broad minimum bactericidal/fungicidal concentration (MBC/MFC) against 12 reference microorganisms. The MIC and MBC/MFC values of AgNPs were 0.312 to 1.25 μg/ml and 0.625 to 10 μg/ml, respectively. Nevertheless, AgNPs did not demonstrate any antagonistic activity against Coxsackie B virus. The in vitro cytotoxicity of the mycosynthesized AgNPs showed significant antitumor activity against adenocarcinoma epithelial cells from human breast cancer (Mcf-7) cell line with an inhibitory concentration (IC50) of 87.5 μg/ml.

scholarly journals Characterization and Antibacterial Response of Silver Nanoparticles Biosynthesized Using an Ethanolic Extract of Coccinia indica Leaves

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 97
Author(s):  
Suresh V. Chinni ◽  
Subash C. B. Gopinath ◽  
Periasamy Anbu ◽  
Neeraj Kumar Fuloria ◽  
Shivkanya Fuloria ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Zeta Potential ◽  
Ethanolic Extract ◽  
Spherical Shape ◽  
Bacterial Strains ◽  
X Ray ◽  
Coccinia Indica ◽  
Antibacterial Potential

The present study was planned to characterize and analyze the antimicrobial activity of silver nanoparticles (AgNP) biosynthesized using a Coccinia indica leaf (CIL) ethanolic extract. The present study included the preparation of CIL ethanolic extract using the maceration process, which was further used for AgNP biosynthesis by silver nitrate reduction. Biosynthetic AgNPs were characterized using UV–Visible spectrometry, zeta potential analysis, transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and energy-dispersive X-ray (EDX) spectrometry. The biogenic AgNP and CIL extracts were further investigated against different bacterial strains for their antimicrobial activity. The surface plasmon resonance (SPR) signal at 425 nm confirmed AgNP formation. The SEM and TEM data revealed the spherical shape of biogenic AgNPs and size in the range of 8 to 48 nm. The EDX results verified the presence of Ag. The AgNPs displayed a zeta potential of −55.46 mV, suggesting mild AgNP stability. Compared to Gram-positive bacteria, the biogenic AgNPs demonstrated high antibacterial potential against Gram-negative bacteria. Based on the results, the current study concluded that AgNPs based on CIL extract have strong antibacterial potential, and it established that AgNP biosynthesis using CIL ethanol extract is an effective process.

scholarly journals Silver Nanoparticles from Oregano Leaves’ Extracts as Antimicrobial Components for Non-Infected Hydrogel Contact Lenses

2021 ◽  
Vol 22 (7) ◽  
pp. 3539
Author(s):  
Anastasia Meretoudi ◽  
Christina N. Banti ◽  
Panagiotis K. Raptis ◽  
Christina Papachristodoulou ◽  
Nikolaos Kourkoumelis ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Contact Lenses ◽  
Attenuated Total Reflection ◽  
Bacterial Strains ◽  
Scanning Calorimetry ◽  
X Ray ◽  
E Coli ◽  
Polymer Hydrogels ◽  
20 Nm ◽  
And Staphylococcus Aureus

The oregano leaves’ extract (ORLE) was used for the formation of silver nanoparticles (AgNPs(ORLE)). ORLE and AgNPs(ORLE) (2 mg/mL) were dispersed in polymer hydrogels to give the pHEMA@ORLE_2 and pHEMA@AgNPs(ORLE)_2 using hydroxyethyl–methacrylate (HEMA). The materials were characterized by X-ray fluorescence (XRF) spectroscopy, X-ray powder diffraction analysis (XRPD), thermogravimetric differential thermal analysis (TG-DTA), derivative thermogravimetry/differential scanning calorimetry (DTG/DSC), ultraviolet (UV-Vis), and attenuated total reflection mode (ATR-FTIR) spectroscopies in solid state and UV–Vis in solution. The crystallite size value, analyzed with XRPD, was determined at 20 nm. The antimicrobial activity of the materials was investigated against Gram-negative bacterial strains Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). The Gram-positive ones of the genus of Staphylococcus epidermidis (S. epidermidis) and Staphylococcus aureus (S. aureus) are known to be involved in microbial keratitis by the means of inhibitory zone (IZ), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC). The IZs, which developed upon incubation of P. aeruginosa, E. coli, S. epidermidis, and S. aureus with paper discs soaked in 2 mg/mL of AgNPs(ORLE), were 11.7 ± 0.7, 13.5 ± 1.9, 12.7 ± 1.7, and 14.3 ± 1.7 mm. When the same dose of ORLE was administrated, the IZs were 10.2 ± 0.7, 9.2 ± 0.5, 9.0 ± 0.0, and 9.0 ± 0.0 mm. The percent of bacterial viability when they were incubated over the polymeric hydrogel discs of pHEMA@AgNPs(ORLE)_2 was interestingly low (66.5, 88.3, 77.7, and 59.6%, respectively, against of P. aeruginosa, E. coli, S. epidermidis, and S. aureus) and those of pHEMA@ORLE_2 were 89.3, 88.1, 92.8, and 84.6%, respectively. Consequently, pHEMA@AgNPs(ORLE)_2 could be an efficient candidate toward the development of non-infectious contact lenses.

Optimization of Green Synthesis Approach of Silver Nanoparticles Using Indonesian Wild Honey

2021 ◽  
Vol 891 ◽  
pp. 111-115
Author(s):  
Maradhana Agung Marsudi ◽  
Farah Fitria Sari ◽  
Pandu Mauliddin Wicaksono ◽  
Adinda Asmoro ◽  
Arif Basuki ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Synthesis Method ◽  
X Ray Diffraction ◽  
X Ray ◽  
Particle Count ◽  
Transmission Electron ◽  
Nitrate Precursor ◽  
Synthesis Approach

In this work, silver nanoparticles have been successfully synthesized using simple and environmentally friendly ‘green synthesis’ method using Indonesian wild honey as mediator. Particle count and size can be optimized by varying the silver nitrate precursor and honey concentration, with the help of sodium hydroxide as pH regulator. Based on X-ray diffraction (XRD) result, crystalline structure of Ag has been confirmed in sample with impurities from AgCl. Based on dynamic light scattering (DLS) and transmission electron microscopy (TEM) results, it was found that the smallest average particles size of AgNPs (117.5 nm from DLS and 11.1 nm from TEM) was obtained at sample with 5% w/v of honey and 0.5 mM of AgNO3.

Synthesis of Nanosized HMS Mesoporous Molecular Sieve in an Ionic Liquid-Water Mixture

2011 ◽  
Vol 236-238 ◽  
pp. 2110-2113
Author(s):  
Hong Liu ◽  
Meng Yang Wang ◽  
Wei Ran Cao
Keyword(s):  
Electron Microscopy ◽  
Ionic Liquid ◽  
Sol Gel ◽  
Mesoporous Molecular Sieve ◽  
Nano Particles ◽  
Water Mixture ◽  
X Ray ◽  
Transmission Electron ◽  
Adsorption Desorption ◽  
Synthesized Materials

The hexagonal mesoporous silica (HMS) nano-particles were prepared in mixture of 1-butyl-3-methyl-imidazolium tetrafluoroborate (BMIM+BF4-) ionic liquid and water by a sol-gel method. The structure and morphology of obtained materials were characterized by X-ray powder diffraction (XRD), N2adsorption-desorption, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and dynamic light scattering (DLS). The influence of the amount of BMIM+BF4-was investigated. It was shown that the synthesized materials have discrete and uniform spherical morphology with the size in the range of 68-177 nm (obtained from DLS measurements), and the particle size of HMS can be controlled by varying the amount of BMIM+BF4-.

scholarly journals SILVER NANOPARTICLES FROM MEDICINALLY IMPORTANT EUPHORBIA CYATHOPHORA EXTRACT: BIOSYNTHESIS, CHARACTERIZATION, AND ANTICANCER ACTIVITY.

2018 ◽  
Vol 11 (2) ◽  
pp. 154
Author(s):  
Robert Lotha ◽  
Aravind Sivasubramanian ◽  
Meenakshi Sundaram Muthuraman
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Cell Line ◽  
Aqueous Extract ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Transmission Electron ◽  
The Stability ◽  
Ht 29 ◽  
Scanning Electron

Objective: The present study was aimed at the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Euphorbia cyathophora leavesand testing their anticancer potential using HT-29 cell line model.Methods: Green synthesis of silver nanoparticles was obtained with the aqueous extract of E. cyathophora. The synthesized nanoparticles wereconfirmed initially by ultraviolet-visible spectroscopy. Further, scanning electron microscopy, transmission electron microscopy, and X-Ray diffractionstudies also ensured the presence of silver nanoparticles. Zeta potential studies revealed the stability of the silver nanoparticles.Results: Antioxidant and anticancer studies of the nanoparticles against HT-29 cell line exhibited remarkable results.Conclusion: This ensures that the synthesized nanoparticles play an important role in medicinal biology.

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