scholarly journals A Fatal Case of Necrotizing Fasciitis Caused by a Highly VirulentEscherichia coliStrain

Author(s):  
Sadjia Bekal ◽  
André Vincent ◽  
Alex Lin ◽  
Josée Harel ◽  
Jean-Charles Côté ◽  
...  

Necrotizing fasciitis is a serious disease characterized by the necrosis of the subcutaneous tissues and fascia.E. colias the etiologic agent of necrotizing fasciitis is a rare occurrence. A 66-year-old woman underwent total abdominal hysterectomy with bilateral salpingo-oophorectomy. She rapidly developed necrotizing fasciitis which led to her death 68 hours following surgery. AnE. colistrain was isolated from blood and fascia cultures. DNA microarray revealed the presence of 20 virulence genes.

2021 ◽  
Author(s):  
Zhen Zhu ◽  
Mingze Cao ◽  
Weiwei Wang ◽  
Qiqi Zhu ◽  
Chengye Wang ◽  
...  

Abstract Background: Avian pathogenic E. coli (APEC) is the etiologic agent of serious colibacillosis and causes extensive economic losses. To examine the genetic background of APEC, we characterized the serotypes, virulence genes, phylogenetic classification and MLST of 392 APEC and 586 AFEC strains isolated from infected chickens. Results: The results showed that the most predominant serotypes were O78 (13.47%), O2 (9.16%), O18 (5.39%), O20 (4.42%) and O25 (4.09%). The major serotypes O78 (13.47%) and O2 (9.16%) were significantly higher in the APEC isolates than in the AFEC isolates. Among the 16 analyzed virulence-associated genes (VAGs), iroN (100%), ompT (100%), fimC (92.46%), iss (77.91%) and irp2 (71.98%) were the most frequently identified. Over half (54.85%) of the strains possessed > 8–13 VAGs, and 85.23% of the strains carried iroN-ompT-fimC-iss/irp2 VAG patterns. According to the phylogenetic analysis, phylogroups A (32.11%) and B2 (31.36%) proved to be the most prevalent phylogenetic groups in the AFEC and APEC isolates, respectively. The strains that belonged to phylogroup B2 were associated with more VAGs. Based on MLST, 46 STs belonging to 15 different clonal complexes were identified, and 4 were novel. ST88 (10.67%) was found to be the most dominant ST, and it possessed at least 9 VAGs and belonged to phylogroups B2 or D. Furthermore, the isolates belonging to B2-O78/O2-ST88 were the most likely APEC isolates to be associated with epidemics, and they carried more VAGs than the other strains. Conclusions: Our findings have enriched our knowledge of the molecular characteristics of APEC isolates from chickens, which will be important for the prevention and control of avian colibacillosis.


Author(s):  
Michelle Fonseca ◽  
Ashwini Desai ◽  
Deepali Kapote ◽  
Swati Gawai ◽  
Anam Syed

Uterine inversion is prolapse of the uterine fundus and corpus turned inside out towards or through the cervix. The non-puerperal type of uterine inversion is a quite rare occurrence and it accounts for only 17% of all uterine inversion cases. We present a case of 45 year old multiparous female who presented to us with polymenorrhagia with fibroid polyp. Ultrasonography confirmed the clinical findings however Intraoperatively examination under anaesthesia revealed uterine inversion. On table we proceeded with Haultains method of uterine reposition followed by total abdominal hysterectomy. Patient recovered well and thus case was successfully managed.


2012 ◽  
Vol 18 (1) ◽  
pp. 39-42 ◽  
Author(s):  
Jahanara Rahman ◽  
Nasreen Sultana ◽  
Munir Hasan ◽  
Hosne Ara Begum

A cross-sectional descriptive study was carried out in Obstetrics and Gynecology department, unit I of Dhaka National Medical College Hospital between June 2010 and May 2011. The objectives of the study were to find out the predisposing factors and to identify the organism causing post-operative wound infection. Out of 530 patients who underwent major abdominal surgery, 40 developed wound infection and were included in this study. Mean age of the population was 29.73 SD± 94, ranges 18 to 60 years, all were female, 75% of them were literate and 55% respondents came from middle class and affluent society. Sixty percent population was multiparous. Anaemia and raised blood sugar was found in 70% and 27% of the population respectively. The respondents under went caesarean section (71%), total abdominal hysterectomy (27%) and laparotomy (2%). Wound infection appeared within 5th day, 6th to 10th day and beyond 10th day of operation in 22.5%, 52.5% and 25% cases respectively. Wound discharge was serosanguinous in 50% cases. Bloody, purulent and no discharge was found in 10%, 30% and 10% wound respectively. Growth of Staphylococcus was noticed in 30% culture wound swab. Besides this, E coli, Pseudomonas, Klebsiella, Mycobacterium tuberculosis and other Coliform bacilli was found in 20%, 5%, 5%, 2.5% and 2.5% of growth. In 35% cases no growth of organism took place. DOI: http://dx.doi.org/10.3329/jdnmch.v18i1.12239 J. Dhaka National Med. Coll. Hos. 2012; 18 (01): 39-42


2020 ◽  
Vol 6 (6) ◽  
pp. 268-270
Author(s):  
Rajasri G Yaliwal ◽  
◽  
Girija Hanjagi ◽  
Shobha Shiragur ◽  
Shivakumar Pujeri ◽  
...  

Incarcerated prolapse in association with bladder calculi is a rare occurrence. Table sugar and salt, which are both hygroscopic agents, have been used to reduce incarcerated bowel prolapse. Table sugar and salt was used in the following case of incarcerated procidencia with vescical calculi. Cystotomy with bladder stone removal was done by abdominal route. Total abdominal hysterectomy with bilateral salpingoopherectomy was done with pelvic floor repair and vault suspension.


2021 ◽  
Vol 22 (11) ◽  
pp. 5905
Author(s):  
Olivia M. Grünzweil ◽  
Lauren Palmer ◽  
Adriana Cabal ◽  
Michael P. Szostak ◽  
Werner Ruppitsch ◽  
...  

Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene blaCMY (n = 51) was the predominant β-lactamase gene. In addition, blaTEM-1 (n = 38), blaSHV-33 (n = 8), blaCTX-M-15 (n = 7), blaOXA-1 (n = 7), blaSHV-11 (n = 3), and blaDHA-1 (n = 2) were detected. The most prevalent non-β-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.


2021 ◽  
Vol 14 (2) ◽  
pp. e238945
Author(s):  
Olga Triantafyllidou ◽  
Stavroula Kastora ◽  
Irini Messini ◽  
Dimitrios Kalampokis

Subinvolution of placental sites (SPSs) is a rare but severe cause of secondary postpartum haemorrhage (PPH). SPS is characterised by the abnormal persistence of large, dilated, superficially modified spiral arteries in the absence of retained products of conception. It is an important cause of morbidity and mortality of young women. In this study, we present a case of secondary PPH in a young woman after uncomplicated caesarean delivery who was deemed clinically unstable, and finally, underwent emergent total abdominal hysterectomy. We reviewed the literature with an emphasis on the pathophysiology of this situation. Treatment of patients with SPS includes conservative medical therapy, hysterectomy and fertility-sparing percutaneous embolotherapy.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pimlapas Leekitcharoenphon ◽  
Markus Hans Kristofer Johansson ◽  
Patrick Munk ◽  
Burkhard Malorny ◽  
Magdalena Skarżyńska ◽  
...  

AbstractThe emergence of antimicrobial resistance (AMR) is one of the biggest health threats globally. In addition, the use of antimicrobial drugs in humans and livestock is considered an important driver of antimicrobial resistance. The commensal microbiota, and especially the intestinal microbiota, has been shown to have an important role in the emergence of AMR. Mobile genetic elements (MGEs) also play a central role in facilitating the acquisition and spread of AMR genes. We isolated Escherichia coli (n = 627) from fecal samples in respectively 25 poultry, 28 swine, and 15 veal calf herds from 6 European countries to investigate the phylogeny of E. coli at country, animal host and farm levels. Furthermore, we examine the evolution of AMR in E. coli genomes including an association with virulence genes, plasmids and MGEs. We compared the abundance metrics retrieved from metagenomic sequencing and whole genome sequenced of E. coli isolates from the same fecal samples and farms. The E. coli isolates in this study indicated no clonality or clustering based on country of origin and genetic markers; AMR, and MGEs. Nonetheless, mobile genetic elements play a role in the acquisition of AMR and virulence genes. Additionally, an abundance of AMR was agreeable between metagenomic and whole genome sequencing analysis for several AMR classes in poultry fecal samples suggesting that metagenomics could be used as an indicator for surveillance of AMR in E. coli isolates and vice versa.


Author(s):  
Sunanda N.

Although leiomyomas are the most common pelvic tumors presenting in the reproductive age group, cervical fibroids are rare accounting for 2% of all uterine fibroids. We report a case of 40 year old lady presenting with a firm, non-tender mass of 22-24 weeks size pregnant uterus with restricted mobility. Laparotomy showed a large mass arising from the anterior lip of cervix, with a small uterus pushed posteriorly. Enucleation followed by total abdominal hysterectomy was done. Large cervical fibroids are rare, presenting with surgical difficulties. Careful dissection by expert hands is needed in the management of such cases. 


Sign in / Sign up

Export Citation Format

Share Document