scholarly journals Histological and Molecular Characterization of Grape Early Ripening Bud Mutant

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Da-Long Guo ◽  
Yi-He Yu ◽  
Fei-Fei Xi ◽  
Yan-Yan Shi ◽  
Guo-Hai Zhang

An early ripening bud mutant was analyzed based on the histological, SSR, and methylation-sensitive amplified polymorphism (MSAP) analysis and a layer-specific approach was used to investigate the differentiation between the bud mutant and its parent. The results showed that the thickness of leaf spongy tissue of mutant (MT) is larger than that of wild type (WT) and the differences are significant. The mean size of cell layer L2 was increased in the mutant and the difference is significant. The genetic background of bud mutant revealed by SSR analysis is highly uniform to its parent; just the variations from VVS2 SSR marker were detected in MT. The total methylation ratio of MT is lower than that of the corresponding WT. The outside methylation ratio in MT is much less than that in WT; the average inner methylation ratio in MT is larger than that in WT. The early ripening bud mutant has certain proportion demethylation in cell layer L2. All the results suggested that cell layer L2 of the early ripening bud mutant has changed from the WT. This study provided the basis for a better understanding of the characteristic features of the early ripening bud mutant in grape.

Author(s):  
Tashatai Prasertpol ◽  
Waree Tiyaboonchai

Objective: This study aimed to develop the nanostructured lipid carriers (NLCs) for repairing the hair split-end and to compare the influence of NLCs charges on hair binding effects. Methods: NLCs was prepared by a high-pressure homogenization technique. The difference solid lipids were selected to obtain the negatively charged NLCs (N-NLCs) and positively charged NLCs (P-NLCs). The physical characterizations of both NLCs were examined. The NLCs cream was prepared by mixing NLCs into a cream base. Then, the hair split-end binding effects of the NLCs cream were assessed. The physical stability of both NLCs were investigated by kept at room temperature for 6 mo. Results: The N-NLCs and P-NLCs were successfully prepared with a zeta potential of -44.4 mV and 32.3 mV, respectively. They were a spherical shape with a similar mean size of ~150-160 nm. The hair split-end evaluation showed that both NLCs illustrated similar hair split-end binding time of more than 3 d, suggesting no extra benefit from the positively charged of P-NLCs. On the contrary, the cream base and commercial product showed a short binding time of <8 h. Furthermore, after 6-mo storage, N-NLCs demonstrated good physical stability without particle aggregation, while P-NLCs exhibited a phase separation. Conclusion: The developed NLCs is a novel alternative for the leave-on product, which would be highly benefits for hair split-end repairing.


Author(s):  
Fawzan Galib Abdul Karim Bawahab ◽  
Elvan Yuniarti ◽  
Edi Kurniawan

Abstrak. Pada penelitian ini, telah dilakukan analisa karakterisasi pada teknologi Direct Sequence Spread Spectrum dan Frequency Hopping Spread Spectrum, sebagai salah satu teknik multiple-access pada sistem komunikasi. Karakterisasi dilakukan untuk mencari bagaimana cara meningkatkan keoptimalan kedua sistem tersebut, dalam mengatasi masalah interferensi dengan sistem dan channel yang sama. Dan juga untuk menentukan veriabel apa yang mempengaruhi keoptimalan kedua sistem tersebut. Karakterisasi dilakukan dengan menentukan variabel-variabel yang mempengaruhi keoptimalan keduanya. Hasil dari karakterisasi, diketahui variabel-variabel yang mempengaruhi kemampuan sistem DSSS yaitu nilai frekuensi spreading (). Sedangkan untuk sistem FHSS yaitu nilai frekuensi spreading ( dan ) dan selisih antara frekuensi hopping data dengan frekuensi hopping interferensi . Kata Kunci: BER, DSSS, FHSS, Interference, Spread spectrum. Abstract. In this study, characterization of Direct Sequence Spread Spectrum and Frequency Hopping Spread Spectrum technologies have been done, as one of the multiple-access techniques in communication systems. Characterization is done to find out how to improve the ability of the two systems, in solving interference problems with the same system and channel. And also to determine what veriabel affects the ability of the two systems. Characterization is done by determining the variables that affect the ability of both. The results of the characterization, known variables that affect the ability of the DSSS system are the spreading frequency value (). As for the FHSS system, the spreading frequency value ( and ) and the difference between frequency hopping data with frequency hopping interference .


1984 ◽  
Vol 49 (2) ◽  
pp. 410-420
Author(s):  
Eva Hillerová ◽  
Miroslav Zdražil

Reversible adsorption of heptane and benzene on model and industrial hydrodesulphurization molybdena catalysts has been studied by elution chromatographic method at 150 °C. An increase in the adsorption of heptane on sulphidation of adsorbents was small for Al2O3 and great for MoO3. Supported catalysts behaved as mixture of Al2O3 and MoO3.The portion of surface which can be transformed by sulphidation into MoS2 ranged from 0 to 65% for individual commercial catalysts, as determined from the change in heptane adsorption after sulphidation of a given sample. The polarity of catalysts, including their acidity, was estimated from the difference between adsorption of benzene and heptane. The polarity of model and industrial catalysts in oxidic form was similar to that of alumina in most cases. The decrease in the polarity after sulphidation of the adsorbents was small for Al2O3 and great for MoO3. The decrease in polarity resulting from sulphidation of supported catalysts was relatively small, since the reaction of MoO3 monolayer with hydrogen sulphide leads to partial reformation of the alumina surface. The acidity of supported sulphided hydrodesulphurization catalysts has been shown by this method to be comparable with the acidity of the support itself.


2004 ◽  
Vol 82 (8) ◽  
pp. 1294-1303 ◽  
Author(s):  
Vanessa Renée Little ◽  
Keith Vaughan

1-Methylpiperazine was coupled with a series of diazonium salts to afford the 1-methyl-4-[2-aryl-1-diazenyl]piperazines (2), a new series of triazenes, which have been characterized by 1H and 13C NMR spectroscopy, IR spectroscopy, and elemental analysis. Assignment of the chemical shifts to specific protons and carbons in the piperazine ring was facilitated by comparison with the chemical shifts in the model compounds piperazine and 1-methylpiperazine and by a HETCOR experiment with the p-tolyl derivative (2i). A DEPT experiment with 1-methylpiperazine (6) was necessary to distinguish the methyl and methylene groups in 6, and a HETCOR spectrum of 6 enabled the correlation of proton and carbon chemical shifts. Line broadening of the signals from the ring methylene protons is attributed to restricted rotation around the N2-N3 bond of the triazene moiety in 2. The second series of triazenes, the ethyl 4-[2-phenyl-1-diazenyl]-1-piperazinecarboxylates (3), have been prepared by similar diazonium coupling to ethyl 1-piperazinecarboxylate and were similarly characterized. The chemical shifts of the piperazine ring protons are much closer together in series 3 than in series 2, resulting in distortion of the multiplets for these methylenes. It was noticed that the difference between these chemical shifts in 3 exhibited a linear free energy relationship with the Hammett substituent constants for the substituents in the aryl ring. Key words: triazene, piperazine, diazonium coupling, NMR, HETCOR, linear free energy relationship.


Morphology ◽  
2021 ◽  
Author(s):  
Rossella Varvara ◽  
Gabriella Lapesa ◽  
Sebastian Padó

AbstractWe present the results of a large-scale corpus-based comparison of two German event nominalization patterns: deverbal nouns in -ung (e.g., die Evaluierung, ‘the evaluation’) and nominal infinitives (e.g., das Evaluieren, ‘the evaluating’). Among the many available event nominalization patterns for German, we selected these two because they are both highly productive and challenging from the semantic point of view. Both patterns are known to keep a tight relation with the event denoted by the base verb, but with different nuances. Our study targets a better understanding of the differences in their semantic import.The key notion of our comparison is that of semantic transparency, and we propose a usage-based characterization of the relationship between derived nominals and their bases. Using methods from distributional semantics, we bring to bear two concrete measures of transparency which highlight different nuances: the first one, cosine, detects nominalizations which are semantically similar to their bases; the second one, distributional inclusion, detects nominalizations which are used in a subset of the contexts of the base verb. We find that only the inclusion measure helps in characterizing the difference between the two types of nominalizations, in relation with the traditionally considered variable of relative frequency (Hay, 2001). Finally, the distributional analysis allows us to frame our comparison in the broader coordinates of the inflection vs. derivation cline.


2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Vladimir Yu. Osipov ◽  
Fedor M. Shakhov ◽  
Kirill V. Bogdanov ◽  
Kazuyuki Takai ◽  
Takuya Hayashi ◽  
...  

Abstract We demonstrate a high-pressure, high-temperature sintering technique to form nitrogen-vacancy-nitrogen centres in nanodiamonds. Polycrystalline diamond nanoparticle precursors, with mean size of 25 nm, are produced by the shock wave from an explosion. These nanoparticles are sintered in the presence of ethanol, at a pressure of 7 GPa and temperature of 1300 °C, to produce substantially larger (3–4 times) diamond crystallites. The recorded spectral properties demonstrate the improved crystalline quality. The types of defects present are also observed to change; the characteristic spectral features of nitrogen-vacancy and silicon-vacancy centres present for the precursor material disappear. Two new characteristic features appear: (1) paramagnetic substitutional nitrogen (P1 centres with spin ½) with an electron paramagnetic resonance characteristic triplet hyperfine structure due to the I = 1 magnetic moment of the nitrogen nuclear spin and (2) the green spectral photoluminescence signature of the nitrogen-vacancy-nitrogen centres. This production method is a strong alternative to conventional high-energy particle beam irradiation. It can be used to easily produce purely green fluorescing nanodiamonds with advantageous properties for optical biolabelling applications.


2021 ◽  
Vol 11 (13) ◽  
pp. 5924
Author(s):  
Elisa Levi ◽  
Simona Sgarbi ◽  
Edoardo Alessio Piana

From a circular economy perspective, the acoustic characterization of steelwork by-products is a topic worth investigating, especially because little or no literature can be found on this subject. The possibility to reuse and add value to a large amount of this kind of waste material can lead to significant economic and environmental benefits. Once properly analyzed and optimized, these by-products can become a valuable alternative to conventional materials for noise control applications. The main acoustic properties of these materials can be investigated by means of a four-microphone impedance tube. Through an inverse technique, it is then possible to derive some non-acoustic properties of interest, useful to physically characterize the structure of the materials. The inverse method adopted in this paper is founded on the Johnson–Champoux–Allard model and uses a standard minimization procedure based on the difference between the sound absorption coefficients obtained experimentally and predicted by the Johnson–Champoux–Allard model. The results obtained are consistent with other literature data for similar materials. The knowledge of the physical parameters retrieved applying this technique (porosity, airflow resistivity, tortuosity, viscous and thermal characteristic length) is fundamental for the acoustic optimization of the porous materials in the case of future applications.


2008 ◽  
Vol 23 (27n30) ◽  
pp. 2512-2515 ◽  
Author(s):  
HIDEKO NAGAHIRO ◽  
DAISUKE JIDO ◽  
SATORU HIRENZAKI

We investigate the properties of η-nucleus interaction by postulating the N*(1535) dominance for η-N system. We evaluate the N*(1535) properties in the nuclear medium using two kinds of chiral models, and find that these two models provide qualitatively different η-nucleus optical potentials reflecting the quite distinct properties of N*(1535) in these chiral models. Especially, in the chiral doublet model, we can expect to have the level crossing between η and N*(1535)-hole which is expected to provide the characteristic features for the optical potential and the formation spectra. We find also that the difference of these models can be seen in the formation cross sections of the η mesic nuclei with (π+, p ) reaction expected to be performed at J-PARC project.


Author(s):  
R. Puente ◽  
G. Paniagua ◽  
T. Verstraete

A multi-objective optimization procedure is applied to the 3D design of a transonic turbine vane row, considering efficiency and stator outlet pressure distortion, which is directly related to induced rotor forcing. The characteristic features that define different individuals along the Pareto Front are described, analyzing the differences between high efficiency airfoils and low interaction. Pressure distortion is assessed by means of a model that requires only of the computation the steady flow field in the domain of the stator. The reduction of aerodynamic rotor forcing is checked via unsteady multistage aerodynamic computations. A well known loss prediction method is used to drive the efficiency of one optimization run, while CFD analysis is used for another, in order to assess the reliability of both methods. In both cases, the decomposition of total losses is performed to quantify the influence on efficiency of reducing rotor forcing. Results show that when striving for efficiency, the rotor is affected by few, but intense shocks. On the other hand, when the objective is the minimization of distortion, multiple shocks will appear.


2003 ◽  
Vol 58 (5-6) ◽  
pp. 363-372 ◽  
Author(s):  
Y. Elerman ◽  
H. Kara ◽  
A. Elmali

The synthesis and characterization of [Cu2(L1)(3,5 prz)] (L1=1,3-Bis(2-hydroxy-3,5-chlorosalicylideneamino) propan-2-ol) 1 and of [Cu2(L2)(3,5 prz)] (L2=1,3-Bis(2-hydroxy-bromosalicylideneamino) propan-2-ol) 2 are reported. The compounds were studied by elemental analysis, infrared and electronic spectra. The structure of the Cu2(L1)(3,5 prz)] complex was determined by x-ray diffraction. The magnetochemical characteristics of these compounds were determined by temperaturedependent magnetic susceptibility measurements, revealing their antiferromagnetic coupling. The superexchange coupling constants are 210 cm−1 for 1 and 440 cm−1 for 2. The difference in the magnitude of the coupling constants was explained by the metal-ligand orbital overlaps and confirmed by ab-initio restricted Hartree-Fock (RHF) calculations. In order to determine the nature of the frontier orbitals, Extended Hückel Molecular Orbital (EHMO) calculations are also reported.


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