scholarly journals Low-Intensity Ultrasound Combined with Hematoporphyrin Monomethyl Ether in the Treatment of Experimental Periodontitis in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Deshu Zhuang ◽  
Zongshan Ji ◽  
Liangjia Bi ◽  
Xiaochun Wang ◽  
Qi Zhou ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Alveolar Bone ◽  
Ultrasound Irradiation ◽  
Monomethyl Ether ◽  
Control Groups ◽  
Hypodermic Injection ◽  
Low Intensity ◽  
Significant Difference ◽  
Low Intensity Ultrasound ◽  
Hematoporphyrin Monomethyl Ether

Objectives. This study aims to evaluate the efficacy of hematoporphyrin monomethyl ether- (HMME-) mediated sonodynamic therapy (SDT) on experimental periodontal disease in rats. Methods. Periodontal disease was induced by submerging ligatures at the first maxillary molar subgingival region in forty-eight male SD rats. After 30 days, the ligatures were removed. The rats were randomly allocated into four groups; the experimental SDT group was treated through hypodermic injection of 40 μg/mL HMME and 3 W/cm2 low-intensity ultrasound irradiation (1 MHz, 600 s). Those in control groups received 40 μg/mL HMME alone (control 1 group) or 3 W/cm2 ultrasound irradiation alone (control 2 group) or were subjected to neither HMME nor ultrasound (control 3 group). After 10 days of treatment, all rats were euthanized, the maxilla was obtained for histological examination, and the alveolar bone level was evaluated by histometric analysis. Results. The control groups showed more bone loss (P<0.05) after 10 days of treatment than the SDT group. There is no significant difference among the control groups (P>0.05). Conclusions. HMME mediated SDT was an effective therapy of experimental periodontal tissue in rats.

Effects of Low Intensity Ultrasound Stimulation on the Proliferation of Alveolar Bone Marrow Stem Cells

2009 ◽  
Author(s):  
Eung Tae Lee ◽  
Ki Taek Lim ◽  
Chong Su Cho ◽  
Jang Ho Kim ◽  
Hyun Mok Son ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Alveolar Bone ◽  
Bone Marrow Stem Cells ◽  
Low Intensity ◽  
Ultrasound Stimulation ◽  
Low Intensity Ultrasound

scholarly journals The pore size of chitosan-Aloe vera scaffold and its effect on VEGF expressions and woven alveolar bone healing of tooth extraction of Cavia cobaya

2020 ◽  
Vol 53 (3) ◽  
pp. 115
Author(s):  
S. Sularsih
Keyword(s):  
Treatment Group ◽  
Pore Size ◽  
Bone Healing ◽  
Tooth Extraction ◽  
Alveolar Bone ◽  
Histopathological Examination ◽  
Aloe Vera ◽  
Control Groups ◽  
Treatment Groups ◽  
Significant Difference

Background: Pore size of scaffolds affects cellular activity, stimulates angiogenetic factors of vascular endothelial growth factor (VEGF), synthesises new blood vessels to regulate migration and proliferation, and accelerates alveolar bone healing of tooth extraction. Purpose: This study aims to analyse the pore size of chitosan-Aloe vera scaffold and its effects on VEGF expression and woven alveolar bone healing of tooth extraction of Cavia cobaya. Methods: 36 male Cavia cobaya, aged 3-3.5 months were divided into six groups: negative control groups (without scaffold), positive control groups (chitosan scaffold), and treatment groups (chitosan-Aloe vera scaffold) on 7- and 14-day observations. Histopathological examination was performed to account the woven alveolar bone areas, and immunohistochemical examination was conducted to examine VEGF expressions on endothelial cells. Data was analysed using a one-way analysis of variance (ANOVA) and least significant difference (LSD) test (p<0.05). Scaffold pore size examination was performed with scanning electron microscope (SEM) with 250x and 500x magnification. Results: Chitosan-Aloe vera scaffold was found to have open pore interconnectivity, the largest pore size was 138.9 μm, while the smallest was 110.5 μm and average pore size was 134.85 μm. The highest expression of VEGF was observed in the treatment group on days 7 (11.5 ±1.39)  and 14 (15.28±1.78), while the largest woven alveolar bone was observed in the treatment group on days 7(17.83±1.47) and 14 (37.67±3.65). Statistically, there was a significant difference between control groups and the treatment groups (p=0.000; p<0.05). Conclusion: Chitosan-Aloe vera scaffold has pore characteristics increasing VEGF expressions and woven alveolar bone areas.

scholarly journals EFFECTIVENESS OF KATUK LEAVES ETHANOL EXTRACT AMOUNT OF OSTEOBLAST AND OSTEOCLAST ON ALVEOLAR BONE IN ORTHODONTIC TREATMENT Research On Alveolar Bone Of Guinea Pigs

2021 ◽  
Vol 1 (Volume 1 No 1) ◽  
pp. 38-48
Author(s):  
Hillda Herawati ◽  
Fahrauk Paramayudh ◽  
Rudi Satria Darwis ◽  
Sarah Syarifah
Keyword(s):  
Guinea Pigs ◽  
Orthodontic Treatment ◽  
Alveolar Bone ◽  
Ethanol Extract ◽  
Control Group ◽  
Control Groups ◽  
Significant Difference ◽  
Bone Remodelling Process ◽  
Treatment Research ◽  
And Control

The optimal achievement of orthodontic treatment is determined by a bone remodelling process involving osteoblast, osteoclasts, and the estrogen hormone. Estrogen deficiency can increase osteoclast age and decrease osteoblast, resulting in an imbalance between osteoclasts and osteoblasts. One natural alternative that can replace the role of the hormone estrogen is phytoestrogens. Sauropus androgynus (L.) Merr (katuk) is a phytoestrogen that contains isoflavones with many similarities with estrogens. This research aims to determine the effectiveness of the various doses of ethanol extract of katuk leaves orally on the number of osteoblasts and osteoclasts. This research was conducted using experimental laboratory methods using 24 female Guinea pigs divided into a control group and three groups with various doses of 39.15 mg/BW, 78.3 mg/BW, and 156.5 mg/BW. The observations made in this test were the number of osteoblasts and osteoclasts on the alveolar guinea pig on day 14 and analysed using the one way ANOVA test (p <0.05). All guinea pigs have applied a rubber separator to the left incisor and given a dose according to the group, and after 14th days, histological preparations were made. The results showed that the highest number of osteoblasts was at a dose of 78.3 mg/BW, and the lowest number of osteoclasts was at a dose of 39.15 mg/BW with values of 15.03 ± 2.27 and 1.73 ± 0.56, respectively. Statistically, the number of osteoblasts between the treatment and control groups significantly differed (p = 0.04), while the number of osteoclasts between the treatment and control groups had no significant difference (p = 0.228). This study concludes that katuk leaves extract has effectiveness in increasing the number of osteoblasts in orthodontic treatment, while the decrease in osteoclasts is not statistically proven.

scholarly journals A Synergistic Bactericidal Effect of Low-Frequency and Low-Intensity Ultrasound Combined with Levofloxacin-Loaded PLGA Nanoparticles on M. Smegmatis in Macrophages

2020 ◽  
Author(s):  
Shuang Xie ◽  
Gangjing Li ◽  
Yuru Hou ◽  
Min Yang ◽  
Fahui Li ◽  
...  
Keyword(s):  
Cell Structure ◽  
Low Frequency ◽  
Ultrasound Irradiation ◽  
Average Diameter ◽  
Bactericidal Effect ◽  
Plga Nanoparticles ◽  
Low Intensity ◽  
Low Intensity Ultrasound ◽  
Underlying Mechanisms

Abstract Purpose: Tuberculosis (TB) is a highly infectious disease caused by Mycobacterium tuberculosis (Mtb), which often parasites in macrophages. This study is performed to investigate the bactericidal effect and underlying mechanisms of low-frequency and low-intensity ultrasound (LFLIU) combined with levofloxacin-loaded PLGA nanoparticles (LEV-NPs) on M. smegmatis (a surrogate of Mtb) in macrophages.Methods and results: The LEV-NPs were prepared using a double emulsification method. The average diameter, zeta potential, polydispersity index, morphology, and drug release efficiency in vitro of the LEV-NPs were investigated. M. smegmatis in macrophages was treated using the LEV-NPs combined with 42 kHz ultrasound irradiation at an intensity of 0.13 W/cm2 for 10 min. The results showed that ultrasound significantly promoted the phagocytosis of nanoparticles by macrophages (p < 0.05). In addition, further ultrasound combined with the LEV-NPs promoted the production of reactive oxygen species (ROS) in macrophage , and the apoptosis rate of the macrophages was significantly higher than that of the control (p < 0.05). The transmission electronic microscope showed that the cell wall of M. smegmatis was ruptured, the cell structure was incomplete, and the bacteria received severe damage in the ultrasound combined with the LEV-NPs group. Activity assays showed that ultrasound combined with the LEV-NPs exhibited a 10-fold higher antibacterial activity against M. smegmatis residing inside macrophages compared with the free drug.Conclusion: These data demonstrated that ultrasound combined with LEV-NPs has great potential as a therapeutic agent for TB.

scholarly journals Effect of Obesity and/or Ligature-induced Periodontitis on Aortic Wall Thickness in Wistar Rats.

2020 ◽  
Vol 33 (1) ◽  
pp. 50-55
Author(s):  
Andressa Moreira ◽  
Alessandra Nicolini ◽  
Eduardo Gaio ◽  
Fernanda Visioli ◽  
Cassiano Rösing ◽  
...  
Keyword(s):  
Body Weight ◽  
Periodontal Disease ◽  
Wall Thickness ◽  
Wistar Rats ◽  
Aortic Wall ◽  
Alveolar Bone ◽  
Cafeteria Diet ◽  
Control Group ◽  
Significant Difference ◽  
Aortic Wall Thickness

The purpose of this study was to evaluate aortic wall thickness after periodontal disease and/or obesity induction in a Wistar rat model.Sixty male Wistar rats were randomly divided into four groups: control (CT), periodontal disease (PD), obesity (OB), and obesity plus periodontal disease (OB+PD). Groups OB and OB+PD received cafeteria diet for 17 weeks. After they had acquired obesity (week 12), periodontal disease was induced by placing a silk ligature on the maxillary right second molar of groups PD and OB+PD. During the experimental period, body weight and Lee index were assessed. Mean alveolar bone loss (ABL) was evaluated, and aortas were prepared for histometric analysis of the aortic wall by ImageJ software. Body weight and Lee index increased in rats exposed to cafeteria diet. Mean ABL was higher in Groups PD and OB+PD than in control and OB (p<0.05). ABL was 18% higher in Group OB+PD than in Group PD, with statistically significant difference (p<0.001). Aortas were thicker in Groups OB and OB+PD than in control and PD groups, respectively (2.31mm ± 0.28 and 2.33 ± 0.29 vs. 2.18 ± 0.26 and 2.14 ± 0.27). Group OB differed significantly from the control group (p=0.036), and OB+PD and OB differed significantly from PD (p=0.004 and p= 0.001, respectively). Obesity alters aortic wall thickness in Wistar rats. However, the presence of periodontal disease did not affect the aortic wall thickness under the conditions of the present study.

Outcome of periodontal–orthodontic treatment in subjects with periodontal disease. Part II: a CBCT study of alveolar bone level changes

2019 ◽  
Vol 41 (6) ◽  
pp. 565-574 ◽  
Author(s):  
Eglė Zasčiurinskienė ◽  
Henrik Lund ◽  
Rune Lindsten ◽  
Henrik Jansson ◽  
Krister Bjerklin
Keyword(s):  
Periodontal Disease ◽  
Orthodontic Treatment ◽  
Alveolar Bone ◽  
Test Group ◽  
Periodontal Treatment ◽  
Control Group ◽  
Computed Tomography Images ◽  
Significant Difference ◽  
Bone Level ◽  
Maxillary Incisors

Abstract Aim To examine alveolar bone level (ABL) changes before (T1) and after (T2) orthodontic treatment (OT) in subjects with periodontal disease. Methods The study included 50 subjects with periodontal disease. All patients received subgingival debridement following baseline examination. Control group patients received final periodontal treatment before the start of OT. For the test group patients final periodontal treatment was performed simultaneous to OT. OT was performed with a straight-wire appliance. Micro-implants or temporary crowns on implants were used for posterior anchorage when needed. ABL measurements of 3821 tooth surfaces were performed on cone beam computed tomography images. Results No difference was observed between mean ABL at T1 and T2. ABL remained unchanged on 69 per cent of surfaces. A mean of 15.6 (SD 7.4) per cent of surfaces experienced ABL gain, and a mean of 15.1 (SD 7.5) per cent was found with ABL loss. Small significant median ABL difference was observed on mesial and distal surfaces (P < 0.001). A significant difference was found between median ABL changes on mesial/distal in comparison to buccal/lingual surfaces (P < 0.01). Significantly more buccal (17.9 %) and lingual (18.5 %) surfaces experienced ABL loss when compared with mesial (11.3 %) and distal (12.0 %) surfaces (P < 0.001). Significant difference was found in the median ABL change of intruded (0.5 (IQR 0.94) mm) and non-intruded (−0.4 (IQR 1.07) mm) maxillary incisors (P = 0.04). Significant median ABL gain was found on the lingual surface of maxillary incisors, which were retroclined more than 8.6 degree and intruded more than 1.6 mm. Conclusions ABL changes after periodontal–orthodontic treatment in patients with periodontal disease were small. ABL gain was more observed on mesial and distal surfaces and ABL loss on buccal and lingual surfaces. Larger orthodontic movements of maxillary incisors influenced ABL gain.

scholarly journals Synergistic bactericidal effect of low-frequency and low-intensity ultrasound combined with levofloxacin-loaded nanoparticles on M. smegmatis in macrophages

2020 ◽  
Author(s):  
Shuang Xie ◽  
Gangjing Li ◽  
Yuru Hou ◽  
Min Yang ◽  
Fahui Li ◽  
...  
Keyword(s):  
Cell Structure ◽  
Low Frequency ◽  
Ultrasound Irradiation ◽  
Average Diameter ◽  
Bactericidal Effect ◽  
In Vitro Drug Release ◽  
Low Intensity ◽  
Low Intensity Ultrasound ◽  
Underlying Mechanisms

Abstract Purpose Tuberculosis is a highly infectious disease caused by Mycobacterium tuberculosis (Mtb) which often parasites in macrophages. The present study was to investigate the bactericidal effect and underlying mechanisms of low-frequency and low-intensity ultrasound (LFLIU) combined with levofloxacin-loaded nanoparticles (LEV-NPs) on M. smegmatis (a surrogate of Mtb )in macrophages. Methods and results LEV-NPs were prepared by a double emulsification method. The characterization, such as average diameter, zeta potential, polydispersity index and morphology, and in-vitro drug release efficiency of LEV-NPs were investigated. M. smegmatis in macrophages was treated by LEV-NPs combined with 42 kHz ultrasound irradiation at an intensity of 0.13 W/cm 2 for 10 min. The results showed ultrasound could significantly promote phagocytosis of nanoparticles by macrophages ( p <0.05), further ultrasound combined with LEV-NPs could promote the production of macrophage ROS, and the apoptosis rate of macrophages was significantly higher than that of the control ( p <0.05). Transmission electronic microscope showed M. smegmatis cell wall was ruptured, the cell structure was incomplete, and the bacteria received severe damage in the ultrasound combined with the LEV-NPs group. Activity assays showed that ultrasound combined with LEV-NPs exhibited 10-fold higher antibacterial activity against M. smegmatis residing inside macrophages compared with free drug. Conclusion Our data demonstrate that ultrasound combined with LEV-NPs have a great potential to therapy of tuberculosis.

scholarly journals Comparison of albumin, total protein, globulin and enzyme activity Creatine Phospho Kinase (CPK) in the Gingival Crevicular Fluid of patients with chronic periodontitis before and after treatment

2018 ◽  
Vol 6 (5) ◽  
Author(s):  
Ali Banihashem Rad ◽  
Shade Saghafi ◽  
Eshagh Hashemi ◽  
Ershad Aghasizadeh ◽  
Fathollah Ebrahimi ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Total Protein ◽  
Alveolar Bone ◽  
Gingival Crevicular Fluid ◽  
Phase 1 ◽  
Immunological Response ◽  
Treatment Phase ◽  
Significant Difference

Introduction: Periodontal disease is an inflammatory disease of the supporting tissues of the teeth caused by certain microorganisms and leads to progressive destruction of the periodontal ligament, alveolar bone with pocket formation or the recession, or both. Periodontal disease is usually based on clinical parameters diagnosed and recorded. Other advanced diagnostic techniques of periodontal disease is evaluation of host response, which includes the study of specific or non-specific mediators such as albumin, globulin and creatine phosphokinase enzyme by biochemical methods or immunological response that as part of the individual periodontal infections are known. Methods: The study population consisted of 30 patients. After observing isolation by cotton rolls, 50 to 100 ml GCF via 30 paper point and wetting with a height of 5 mm of the area with more involvement in terms of standard of patients with moderate periodontitis referred to Periodontics ward of Dental School in Mashhad pre-treatment phase 1 (scaling and root planning) and 4 weeks after the treatment is provided. Then changes in albumin, total protein, globulin and CPK enzyme activity in the GCF using biochemical kits measured. Result: In our study, mean CPK in patients with chronic periodontitis before treatment was further after treatment but not significantly different, but the average amount of albumin, total protein and globulin chronic periodontitis patients before treatment lesser than after treatment but there was no significant difference either. Conclusion: In this study, we showed that albumin, total protein and globulin was significantly increased after treatment of periodontitis patients.

scholarly journals A synergistic bactericidal effect of low-frequency and low-intensity ultrasound combined with levofloxacin-loaded PLGA nanoparticles on M. smegmatis in macrophages

2020 ◽  
Author(s):  
Shuang Xie ◽  
Gangjing Li ◽  
Yuru Hou ◽  
Min Yang ◽  
Fahui Li ◽  
...  
Keyword(s):  
Cell Structure ◽  
Low Frequency ◽  
Ultrasound Irradiation ◽  
Average Diameter ◽  
Bactericidal Effect ◽  
Plga Nanoparticles ◽  
Low Intensity ◽  
Low Intensity Ultrasound ◽  
Underlying Mechanisms

Abstract Purpose: Tuberculosis (TB) is a highly infectious disease caused by Mycobacterium tuberculosis (Mtb), which often parasites in macrophages. This study is performed to investigate the bactericidal effect and underlying mechanisms of low-frequency and low-intensity ultrasound (LFLIU) combined with levofloxacin-loaded PLGA nanoparticles (LEV-NPs) on M. smegmatis (a surrogate of Mtb) in macrophages.Methods and results: The LEV-NPs were prepared using a double emulsification method. The average diameter, zeta potential, polydispersity index, morphology, and drug release efficiency in vitro of the LEV-NPs were investigated. M. smegmatis in macrophages was treated using the LEV-NPs combined with 42 kHz ultrasound irradiation at an intensity of 0.13 W/cm2 for 10 min. The results showed that ultrasound significantly promoted the phagocytosis of nanoparticles by macrophages (p < 0.05). In addition, further ultrasound combined with the LEV-NPs promoted the production of reactive oxygen species (ROS) in macrophage, and the apoptosis rate of the macrophages was significantly higher than that of the control (p < 0.05). The transmission electronic microscope showed that the cell wall of M. smegmatis was ruptured, the cell structure was incomplete, and the bacteria received severe damage in the ultrasound combined with the LEV-NPs group. Activity assays showed that ultrasound combined with the LEV-NPs exhibited a 10-fold higher antibacterial activity against M. smegmatis residing inside macrophages compared with the free drug.Conclusion: These data demonstrated that ultrasound combined with LEV-NPs has great potential as a therapeutic agent for TB.

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